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An Ethylene-induced CDNA Encoding A Lipase Expressed At The Onset Of Senescence.
Y. Hong, T. W. Wang, K. Hudak, F. Schade, C. D. Froese, J. Thompson
Published 2000 · Medicine, Biology
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A cDNA clone encoding a lipase (lipolytic acyl hydrolase) expressed at the onset of petal senescence has been isolated by screening a cDNA expression library prepared from carnation flowers (Dianthus caryophyllus). The cDNA contains the lipase consensus sequence, ITFAGHSLGA, and encodes a 447-amino acid polypeptide with a calculated molecular mass of 50.2 kDa that appears to be a cytosolic protein. Over-expression of the clone in Escherichia coli yielded a protein of the expected molecular weight that proved capable of deesterifying fatty acids from p-nitrophenylpalmitate, tri-linolein, soybean phospholipid, and Tween in both in vitro and in situ assays of enzyme activity. The abundance of the lipase mRNA increases just as carnation flowers begin to senesce, and expression of the gene is also induced by treatment with ethylene. Southern blot analyses of carnation genomic DNA have indicated that the lipase is a single copy gene. The lipase gene is also expressed in carnation leaves and is up-regulated when the leaves are treated with ethylene. Deesterification of membrane lipids and ensuing loss of membrane structural integrity are well established early events of plant senescence, and the expression pattern of this lipase gene together with the lipolytic activity of its cognate protein indicate that it plays a fundamentally central role in mediating the onset of senescence.
This paper references
An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
C. Puissant (1990)
Senescence and the Fluidity of Rose Petal Membranes : RELATIONSHIP TO PHOSPHOLIPID METABOLISM.
A. Borochov (1982)
Evidence for early changes in membrane structure during post-harvest development of cut carnation (Dianthus caryophyllus L.) flowers.
G. Paliyath (1990)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
Acceleration of membrane senescence in cut carnation flowers by treatment with ethylene.
J. Thompson (1982)
Lipase in the Lipid Bodies of Corn Scutella during Seedling Growth.
Y. H. Lin (1983)
A simple and sensitive colorimetric method for the determination of long-chain free fatty acids in subcellular organelles.
M. Nixon (1979)
Subcellular Localization of Secondary Lipid Metabolites Including Fragrance Volatiles in Carnation Petals
K. Hudak (1997)
Induction of an extracellular esterase from Candida albicans and some of its properties.
R. Tsuboi (1996)
Senescence in cut carnation flowers: Temporal and physiological relationships among water status, ethylene, abscisic acid and membrane permeability
J. Eze (1986)
Membrane Fluidity in Senescing Apples: Effects of Temperature and Calcium,,
G. Paliyath (1984)
Distinct cis-acting sequences are required for the germination and sugar responses of the cucumber isocitrate lyase gene.
L. De Bellis (1997)
Flotation of lipid‐protein particles containing triacylglycerol and phospholipid from the cytosol of carnation petals
K. Hudak (1996)
THE ROLE OF FREE RADICALS IN SENESCENCE AND WOUNDING
J. Thompson (1987)
Ethylene formation from 1-aminocyclopropane-1-carboxylic acid by microsomal membranes from senescing carnation flowers
S. Mayak (2004)
Involvement of Calcium and Calmodulin in Membrane Deterioration during Senescence of Pea Foliage.
Y. Y. Leshem (1984)
Lipid phase separation mediates binding of porcine pancreatic phospholipase A2 to its substrate.
E. Goormaghtigh (1981)
Lipid Breakdown in Smooth Microsomal Membranes from Bean Cotyledons Alters Membrane Proteins and Induces Proteolysis
C. L. Duxbury (1991)
Senescence-dependent increase in the permeability of liposomes prepared from bean cotyledon membranes.
R. F. Barber (1980)
Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media
G. Pencreac'h (1996)
Nonsedimentable microvesicles from senescing bean cotyledons contain gel phase-forming phospholipid degradation products.
K. Yao (1991)
Common induction and regulation of biphenyl, xylene/toluene, and salicylate catabolism in Pseudomonas paucimobilis.
K. Furukawa (1983)
Lipid metabolism during plant senescence.
J. Thompson (1998)
Membrane deterioration in senescing carnation flowers : coordinated effects of phospholipid degradation and the action of membranous lipoxygenase.
M. Fobel (1987)
Microviscosity parameters and protein mobility in biological membranes.
M. Shinitzky (1976)
Hormone-sensitive lipase--a multipurpose enzyme in lipid metabolism.
S. J. Yeaman (1990)
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Marianne T Hopkins (2007)
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Rui Miao (2019)
Expression and Regulation of Senescence-related Genes in Carnation Flowers with Low Ethylene Production during Senescence
K. Tanase (2013)
Characterization of plasma membrane domains enriched in lipid metabolites.
E. Madey (2001)
A Gene Encoding an Acyl Hydrolase Is Involved in Leaf Senescence in Arabidopsis Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010422.
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Ozone-induced ethylene production is dependent on salicylic acid, and both salicylic acid and ethylene act in concert to regulate ozone-induced cell death.
M. V. Rao (2002)
Mechanism of Senescence in Carnation Flowers
S. Satoh (2005)
Integrated Signaling in Flower Senescence
S. Tripathi (2007)
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E. Skipper (2010)
Plant phospholipase A: advances in molecular biology, biochemistry, and cellular function
Guanqun Chen (2013)
Postharvest Physiology of Cut Carnation Flowers
A. Ebrahimzadeh (2008)
Genômica funcional da interação cacaueiro (Theobroma cacao L.) x Moniliophthora perniciosa por meio do sistema modelo Micro-Tom (Solanum lycopersicum L)
D. Scotton (2012)
Lipases: Sources, Characteristics and application in Food industry
Farzad Mardani kataki (2016)
sPLA2 and PLA1: Secretory Phospholipase A2 and Phospholipase A1 in Plants
H. Kim (2014)
Flower senescence: some molecular aspects
Waseem Shahri (2013)
Functional Analysis of Arabidopsis Lipase Genes
A. K. Padham (2003)
Molecular Mechanisms of Phosphorus Metabolism and Transport during Leaf Senescence
Kyla A. Stigter (2015)
An insight into plant lipase research - challenges encountered.
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M. Leverentz (2002)
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M. V. Rao (2001)
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