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Specific Localization Of A Plant Cell Wall Glycine-rich Protein In Protoxylem Cells Of The Vascular System.

B. Keller, M. Templeton, C. Lamb
Published 1989 · Biology, Medicine

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An antibody against glycine-rich protein 1.8 of bean (Phaseolus vulgaris L.) was used for immunogold/silver localization of the protein in different organs of the plant. In hypocotyls, ovaries, and seed coats, the protein was found specifically in xylem cells of the vascular tissue. In hypocotyls, only protoxylem cells were labeled with the antibody, which indicates a remarkable cell-type specificity for accumulation of this cell wall protein. In mature hypocotyls, the protein was restricted to the same subset of xylem cells but was no longer detected on tissue prints, where a positive antibody reaction depends on the transfer of soluble material from plant tissue to the nitrocellulose filter. This indicates that the glycine-rich protein is insolubilized in the cell wall during development. In longitudinal sections of tracheary elements of young hypocotyls and seed coats, the antibody stained a pattern very similar to that of the lignified secondary thickenings of the cell wall, which suggests a close functional relationship between glycine-rich protein and lignin deposition during cell wall biogenesis in protoxylem cells.

This paper is referenced by
Tissue and subcellular immunolocalisation of enzymes of lignin synthesis in differentiating and wounded hypocotyl tissue of French bean (Phaseolus vulgaris L.)
C. Smith (2004)
Molecular Responses to Water-Deficit Stress in Woody Plants
M. Dias (1999)
Molecular Cloning and Characterization of cDNAs Associated with Tracheary Element Differentiation in
T. Demura (1993)
Constitutive and inducible type 1 ribosome‐inactivating proteins (RIPs) in elderberry (Sambucus nigra L.)
F. M. de Benito (1998)
A maize glycine-rich protein is synthesized in the lateral root cap and accumulates in the mucilage.
T. Matsuyama (1999)
Specific interaction of the tomato bZIP transcription factor VSF-1 with a non-palindromic DNA sequence that controls vascular gene expression
C. Ringli (2004)
Control of Lignin Biosynthesis
J. H. Christensen (2000)
Molecular and morphological aspects of lodging resistance in spring wheat (Triticum aestivum L.)
Urs Zuber (1994)
The glycine-rich cell wall proteins of higher plants.
C. M. Condit (1990)
Apical and lateral shoot apex-specific expression is conferred by promoter of the seed storage protein β-phaseolin gene
P. Sen (2005)
Root-specific expression of genes for novel glycine-rich proteins cloned by use of an antiserum against xylem sap proteins of cucumber.
C. Sakuta (1998)
Tissue-Specific Expression of Cell Wall Proteins in Developing Soybean Tissues.
Z. Ye (1991)
Cell Wall Biosynthesis and Its Regulation
K. Iiyama (1993)
Plasma Membrane-Cell-Wall Adhesion and its Role in Response to Biotic and Abiotic Stresses
S. E. Wyatt (1995)
Immunocytochemical characterization of early-developing flax fiber cell walls
C. Andème-Onzighi (2005)
A glycine-rich protein that facilitates exine formation during tomato pollen development
Kenneth J. McNeil (2009)
Structure and Expression of Genes Coding for Structural Proteins of the Plant Cell
Matilde (2007)
Increasing cellulose production and transgenic plant growth in forest tree species
Tang Wei (2008)
Vascular expression of the grp1.8 promoter is controlled by three specific regulatory elements and one unspecific activating sequence
B. Keller (2004)
A Nove 1 Extracellular Matrix Protein from Tomato Associated with Lignified Secondary Cell WalIs
C. Domingo (2002)
Sec14-like proteins, PATELLIN1 and PATELLIN2, are essential for procambial pattern establishment in Arabidopsis thaliana
Elze Rackaityte (2013)
The Pennsylvania State University
Michelle K. Ludlow (2000)
Localization of repetitive proline-rich proteins in the extracellular matrix of pea root nodules
D. J. Sherrier (2005)
Patterns of soybean proline-rich protein gene expression.
R. Wyatt (1992)
A novel approach to facilitate accessibility of cellulose and hemicellulose: characterization of hybrid poplar transformed with a tyrosine rich peptide gene A Final Report Submitted to The Southeastern Sun Grant Center
H. Liang (2009)
Investigating Biochemical and Developmental Dependencies of Lignification with a Click-Compatible Monolignol Analog in Arabidopsis thaliana Stems
J. Pandey (2016)
Protoxylem: the deposition of a network containing glycine-rich cell wall proteins starts in the cell corners in close association with the pectins of the middle lamella
U. Ryser (2002)
Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose
Jaana Kuuskeri (2016)
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Mi Kwon (1999)
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R. Zhong (2009)
Differential gene expression in Phytophthora infestans during pathogenesis on potato
C. Pieterse (1993)
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Erin D. Scully (2013)
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