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Extracellular Matrix And Hormones Transcriptionally Regulate Bovine Beta-casein 5' Sequences In Stably Transfected Mouse Mammary Cells.

C. Schmidhauser, M. Bissell, C. A. Myers, G. Casperson
Published 1990 · Biology, Medicine

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Milk protein regulation involves synergistic action of lactogenic hormones and extracellular matrix (ECM). It is well established that substratum has a dramatic effect on morphology and function of mammary cells. The molecular mechanisms that regulate the ECM- and hormone-dependent gene expression, however, have not been resolved. To address this question, a subpopulation (designated CID 9) of the mouse mammary epithelial cell strain COMMA-1D has been developed in which more than 35% of the cells express beta-casein, form alveoli-like structures when plated onto a reconstituted basement membrane, and secrete beta-casein unidirectionally into a lumen. These cells were stably transfected with a series of chloramphenicol acetyltransferase (CAT) fusion genes to study transcriptional regulation of the bovine beta-casein gene. The expression of CAT in these lines demonstrated a striking matrix and hormone dependency (greater than 150-fold induction in some cases). This regulation occurred primarily at the transcriptional level and was dependent on the length of the 5' flanking region of the beta-casein promotor. Both matrix and hormonal control of transcription occurred within at least the first 1790 base pairs upstream and/or 42 base pairs downstream of the transcriptional initiation site. The ECM effect was independent of glucocorticoid stimulation. However, prolactin was essential and hydrocortisone further increased CAT expression. Endogenous beta-casein expression in these lines was similar to that of the parent CID 9 cells. Our data indicate the existence of matrix-dependent elements that regulate transcription.
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This paper is referenced by
Integrin-dependent induction of early growth response genes in capillary endothelial cells.
L. Dike (1996)
10.1242/jcs.00656
ECM-induced gap junctional communication enhances mammary epithelial cell differentiation
M. El-Sabban (2003)
10.1016/S0248-4900(99)80068-9
The importance of intermediate filaments in the adaptation of tissues to mechanical stress: evidence from gene knockout studies.
M. Galou (1997)
10.1093/ICB/37.2.195
Gene Regulation by Thyroid Hormone During Amphibian Metamorphosis: Implications on the Role of Cell-Cell and Cell-Extracellular Matrix Interactions
M. Stolow (1997)
10.1007/0-306-46832-8_17
Synergistic and antagonistic interactions of transcription factors in the regulation of milk protein gene expression. Mechanisms of cross-talk between signalling pathways.
W. Doppler (2000)
Anti-inflammatory bio-activities in water extract of Centaurea ainetensis
R. Talhouk (2007)
10.1126/science.7531366
Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix
N. Boudreau (1995)
10.5772/50777
Regulation of Mammary Development as It Relates to Changes in Milk Production Efficiency
E. Wall (2012)
10.1146/ANNUREV.CELLBIO.22.010305.104315
Of extracellular matrix, scaffolds, and signaling: tissue architecture regulates development, homeostasis, and cancer.
C. Nelson (2006)
10.1128/MCB.18.4.2184
Characterization of BCE-1, a Transcriptional Enhancer Regulated by Prolactin and Extracellular Matrix and Modulated by the State of Histone Acetylation
C. Myers (1998)
Mammary gland morphogenesis is inhibited in transgenic mice that overexpress cell surface beta1,4-galactosyltransferase.
H. Hathaway (1996)
10.1242/jcs.00855
A role for the cytoskeleton in prolactin-dependent mammary epithelial cell differentiation
Ghada S Zoubiane (2004)
10.1007/978-1-4615-4295-7_13
Establishment of Mouse Mammary Cell Lines
D. Medina (2000)
10.1016/S0739-7240(01)00110-2
Partial purification and characterization of proteins with growth promoting activities from ovine mammary gland secretions.
R. Talhouk (2001)
10.1016/B978-0-08-092599-8.50012-2
The Actions of Steroid Hormones on Animal Cells in Culture: Mechanisms and Applications
P. Darbre (1994)
10.1016/S1874-6020(96)80010-3
The cytoskeleton in the biology of bone cells
A. Lomri (1996)
10.1091/MBC.10.9.2817
Division of Labor among the α6β4 Integrin, β1 Integrins, and an E3 Laminin Receptor to Signal Morphogenesis and β-Casein Expression in Mammary Epithelial Cells
J. Muschler (1999)
10.1210/ENDO.141.8.7607
Laminin inhibits estrogen action in human breast cancer cells.
T. L. Woodward (2000)
10.1080/0265203031000138231
Functional consequence of exposure to dieldrin on mammary development and function
C. Tarraf (2003)
10.1074/jbc.M610316200
Extracellular Matrix-regulated Gene Expression Requires Cooperation of SWI/SNF and Transcription Factors*
R. Xu (2007)
10.1083/JCB.118.5.1271
Coordinated expression of extracellular matrix-degrading proteinases and their inhibitors regulates mammary epithelial function during involution
R. Talhouk (1992)
Down-regulation of tenascin expression by antiprogestins during terminal differentiation of rat mammary tumors.
G. Vollmer (1992)
Tissue structure, nuclear organization, and gene expression in normal and malignant breast.
M. Bissell (1999)
REGULATION OF CYTOSOLIC NADP + - DEPENDENT ISOCITRATE DEHYDROGENASE EXPRESSION
W. Liu (2006)
10.1016/B978-0-12-397175-3.00046-6
Lactation and Its Hormonal Control
S. Anderson (2015)
10.1007/978-94-011-2354-9_12
Functional interplay between extracellular matrix and extracellular matrix-degrading proteinases in the mammary gland: a coordinate system for regulating mammary epithelial function
R. Talhouk (1992)
10.1016/0960-0760(93)90053-Y
The activated mammary gland specific nuclear factor (MGF) enhances in vitro transcription of the β-casein gene promoter
B. Happ (1993)
10.1002/(SICI)1097-4652(199704)171:1<11::AID-JCP2>3.0.CO;2-K
Separated human breast epithelial and myoepithelial cells have different growth factor requirements in vitro but can reconstitute normal breast lobuloalveolar structure
J. Gomm (1997)
10.3920/978-90-8686-537-6
Indicators of milk and beef quality.
J. Hocquette (2005)
10.1271/bbb.70.93
Analysis of Tissue-Specific Expression of Human Type II Collagen cDNA Driven by Different Sizes of the Upstream Region of the β-Casein Promoter
K. Naruse (2006)
10.1002/(SICI)1097-4644(1998)72:30/31+<250::AID-JCB31>3.0.CO;2-3
Communication between the cell membrane and the nucleus: Role of protein compartmentalization
S. Lelièvre (1998)
10.1007/978-1-4615-3770-0_13
Collagen and Other Matrix Glycoproteins in Embryogenesis
E. Hay (1991)
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