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The High Mobility Group Protein Abf2p Influences The Level Of Yeast Mitochondrial DNA Recombination Intermediates In Vivo.
D. MacAlpine, P. Perlman, R. Butow
Published 1998 · Medicine, Biology
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Abf2p is a high mobility group (HMG) protein found in yeast mitochondria that is required for the maintenance of wild-type (rho+) mtDNA in cells grown on fermentable carbon sources, and for efficient recombination of mtDNA markers in crosses. Here, we show by two-dimensional gel electrophoresis that Abf2p promotes or stabilizes Holliday recombination junction intermediates in rho+ mtDNA in vivo but does not influence the high levels of recombination intermediates readily detected in the mtDNA of petite mutants (rho-). mtDNA recombination junctions are not observed in rho+ mtDNA of wild-type cells but are elevated to detectable levels in cells with a null allele of the MGT1 gene (Deltamgt1), which codes for a mitochondrial cruciform-cutting endonuclease. The level of recombination intermediates in rho+ mtDNA of Deltamgt1 cells is decreased about 10-fold if those cells contain a null allele of the ABF2 gene. Overproduction of Abf2p by >/= 10-fold in wild-type rho+ cells, which leads to mtDNA instability, results in a dramatic increase in mtDNA recombination intermediates. Specific mutations in the two Abf2p HMG boxes required for DNA binding diminishes these responses. We conclude that Abf2p functions in the recombination of rho+ mtDNA.
This paper references
Nonhistone proteins HMG1 and HMG2 change the DNA helical structure.
K. Javaherian (1978)
What determines whether chromosomes segregate reductionally or equationally in meiosis?
G. Simchen (1993)
A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria.
J. F. Diffley (1991)
Type I elements mediate replication fork pausing at conserved upstream sites in the Tetrahymena thermophila ribosomal DNA minichromosome.
D. MacAlpine (1997)
Binding of histones H1 and H5 and their globular domains to four-way junction DNA.
P. Varga-Weisz (1994)
Trans action and the var1 determinant region on yeast mitochondrial DNA. Specific labeling of mitochondrial translation products in zygotes.
I. C. Lopez (1981)
HIV-1 cDNA Integration: Requirement of HMG I(Y) Protein for Function of Preintegration Complexes In Vitro
C. Farnet (1997)
Analysis of mitochondrial DNA nucleoids in wild-type and a mutant strain of Saccharomyces cerevisiae that lacks the mitochondrial HMG box protein Abf2p.
S. M. Newman (1996)
Holliday Junctions Accumulate in Replication Mutants via a RecA Homolog-Independent Mechanism
H. Zou (1997)
Structural analysis of mitochondrial DNA molecules from fungi and plants using moving pictures and pulsed-field gel electrophoresis.
A. Bendich (1996)
Addition of a 29 residue carboxyl-terminal tail converts a simple HMG box-containing protein into a transcriptional activator.
D. J. Dairaghi (1995)
Two mutations in the HMG‐box with very different structural consequences provide insights into the nature of binding to four‐way junction DNA.
S. Teo (1995)
The localization of replication origins on ARS plasmids in S. cerevisiae
B. Brewer (1987)
Functions of the high mobility group protein, Abf2p, in mitochondrial DNA segregation, recombination and copy number in Saccharomyces cerevisiae.
O. Zelenaya-Troitskaya (1998)
Identification and characterization of yeast mutants and the gene for a cruciform cutting endonuclease.
S. Kleff (1992)
Homologous recombination‐dependent initiation of DNA replication from DNA damage‐inducible origins in Escherichia coli.
T. Asai (1993)
A role for recombination junctions in the segregation of mitochondrial DNA in yeast
D. Lockshon (1995)
Rolling circle replication of DNA in yeast mitochondria.
R. Maleszka (1991)
Stimulation of V(D)J cleavage by high mobility group proteins
D. V. van Gent (1997)
Tetrazolium overlay technique for population studies of respiration deficiency in yeast.
M. Ogur (1957)
Distinct roles for two purified factors in transcription of Xenopus mitochondrial DNA.
I. Antoshechkin (1995)
Recombination associated with replication of malarial mitochondrial DNA.
P. Preiser (1996)
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
M. Parisi (1991)
The in vivo replication origin of the yeast 2μm plasmid
J. Huberman (1987)
DNA Looping by Saccharomyces cerevisiae High Mobility Group Proteins NHP6A/B
T. Paull (1995)
A test of the transcription model for biased inheritance of yeast mitochondrial DNA.
H. E. Lorimer (1995)
Two-dimensional gel analysis of rolling circle replication in the presence and absence of bacteriophage T4 primase.
K. D. Belanger (1996)
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Heidi M Blank (2008)
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R. Butow (1999)
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T. Sedman (2005)
Mitochondria-nucleus network for genome stability.
Aneta Kaniak-Golik (2015)
Packaging of single DNA molecules by the yeast mitochondrial protein Abf2p.
L. Brewer (2003)
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C. Birky (2001)
Homologous Strand Exchange and DNA Helicase Activities in Plant Mitochondria
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S. Kuusk (2006)
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