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Bax-induced Cell Death In Tobacco Is Similar To The Hypersensitive Response.
C. Lacomme, S. Santa Cruz
Published 1999 · Medicine, Biology
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Bax, a death-promoting member of the Bcl-2 family of proteins, triggered cell death when expressed in plants from a tobacco mosaic virus vector. Analysis of Bax deletion mutants demonstrated a requirement for the BH1 and BH3 domains in promoting rapid cell death, whereas deletion of the carboxyl-terminal transmembrane domain completely abolished the lethality of Bax in plants. The phenotype of cell death induced by Bax closely resembled the hypersensitive response induced by wild-type tobacco mosaic virus in tobacco plants carrying the N gene. The cell death-promoting function of Bax in plants correlated with accumulation of the defense-related protein PR1, suggesting Bax activated an endogenous cell-death program in plants. In support of this view, both N gene- and Bax-mediated cell death was blocked by okadaic acid, an inhibitor of protein phosphatase activity. The ability of Bax to induce cell death and a defense reaction in plants suggests that some features of animal and plant cell death processes may be shared.
This paper references
The Involvement of Protein Phosphatases in the Activation of ICE/CED-3 Protease, Intracellular Acidification, DNA Digestion, and Apoptosis*
S. J. Morana (1996)
Caspases and programmed cell death in the hypersensitive response of plants to pathogens
Olga María del Pozo (1998)
Heterodimerization-independent Functions of Cell Death Regulatory Proteins Bax and Bcl-2 in Yeast and Mammalian Cells*
H. Zha (1997)
Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance.
H. Padgett (1993)
Lacomme and Santa Cruz Proc
S. Chaudhuri B. Manon (1999)
Programmed Cell Death in Plants.
R. Pennell (1997)
Release of cytochrome c and decrease of cytochrome c oxidase in Bax‐expressing yeast cells, and prevention of these effects by coexpression of Bcl‐xL
S. Manon (1997)
The proto-oncogene Bcl-2 and its role in regulating apoptosis
G. Kroemer (1997)
Lesion growth and virus localization in leaves of Nicotiana tabacum cv. Xanthi nc. after inoculation with tobacco mosaic virus and incubation alternately at 22°C and 32°C
E. A. Weststeijn (1981)
Role of CED-4 in the activation of CED-3
A. Chinnaiyan (1997)
Pathogen-induced programmed cell death in tobacco.
R. Mittler (1997)
The BH3 domain of Bax is sufficient for interaction of Bax with itself and with other family members and it is required for induction of apoptosis.
M. Simonen (1997)
The NB-ARC domain: a novel signalling motif shared by plant resistance gene products and regulators of cell death in animals
E. A. Biezen (1998)
Regulation of cell death protease caspase-9 by phosphorylation.
M. H. Cardone (1998)
The product of the tobacco mosaic virus resistance gene N: Similarity to toll and the interleukin-1 receptor
S. Whitham (1994)
Inhibition of Programmed Cell Death in Tobacco Plants during a Pathogen-Induced Hypersensitive Response at Low Oxygen Pressure.
R. Mittler (1996)
Bcl-2 and Bax function independently to regulate cell death
C. Knudson (1997)
Structure-function comparisons of the proapoptotic protein Bax in yeast and mammalian cells.
H. Zha (1996)
Programmed cell death in plant disease: the purpose and promise of cellular suicide.
D. Gilchrist (1998)
Assembly and movement of a plant virus carrying a green fluorescent protein overcoat.
S. S. Cruz (1996)
New aspects of the mechanism of DNA fragmentation in apoptosis.
P. Walker (1997)
Serine/threonine protein phosphatase is required for tobacco mosaic virus-mediated programmed cell death.
D. Dunigan (1995)
Jellyfish green fluorescent protein as a reporter for virus infections.
D. Baulcombe (1995)
The N gene of tobacco confers resistance to tobacco mosaic virus in transgenic tomato.
S. Whitham (1996)
Programmed Cell Death in Animal Development
M. D. Jacobson (1997)
Cleavage of Nuclear DNA into Oligonucleosomal Fragments during Cell Death Induced by Fungal Infection or by Abiotic Treatments.
D. Ryerson (1996)
Calcium-mediated apoptosis in a plant hypersensitive disease resistance response
A. Levine (1996)
Systemic expression of a bacterial gene by a tobacco mosaic virus-based vector.
J. Donson (1991)
Virus‐mediated delivery of the green fluorescent protein to the endoplasmic reticulum of plant cells
P. Boevink (1996)
Apoptosis: A Functional Paradigm for Programmed Plant Cell Death Induced by a Host-Selective Phytotoxin and Invoked during Development.
H. Wang (1996)
Proapoptotic Protein Bax Heterodimerizes with Bcl-2 and Homodimerizes with Bax via a Novel Domain (BH3) Distinct from BH1 and BH2 (*)
H. Zha (1996)
Regulation of Protein Phosphatase 2A Activity by Caspase-3 during Apoptosis*
M. Santoro (1998)
Okadaic acid, a protein phosphatase inhibitor, blocks calcium changes, gene expression, and cell death induced by gibberellin in wheat aleurone cells.
A. Kuo (1996)
Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism
T. Haystead (1989)
Mitochondria and apoptosis.
D. Green (1998)
Lacomme and Santa Cruz Proc
R. Mittler (1999)
Primary structure of the Aequorea victoria green-fluorescent protein.
D. Prasher (1992)
PROGRAMMED CELL DEATH IN PLANT-PATHOGEN INTERACTIONS.
J. Greenberg (1997)
Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death
Zoltán N. Oltval (1993)
VIRUS-HOST INTERACTIONS: Induction of Chlorotic and Necrotic Responses in Plants by Tobamoviruses
J. Culver (1991)
Pathogenesis-related proteins in plants
U. Pfitzner (1999)
The hypersensitive reaction in plants to pathogens: a resistance phenomenon.
R. Goodman (1994)
Apoptotic Pathways The Roads to Ruin
D. Green (1998)
This paper is referenced by
Cell Death in Plant Disease
D. Pontier (2004)
Effect of mitochondria-targeted antioxidant SkQ1 on programmed cell death induced by viral proteins in tobacco plants
A. D. Solovieva (2013)
Identification of Novel Pepper Genes Involved in Bax- or INF1-Mediated Cell Death Responses by High-Throughput Virus-Induced Gene Silencing
Jeong Hee Lee (2013)
Host-selective toxins and avirulence determinants: what's in a name?
T. Wolpert (2002)
Identification and characterisation of genes which underpin non-host disease resistance in Arabidopsis
Jeong-Gu Kang (2007)
Programmed Cell Death as a Response to High Light, UV and Drought Stress in Plants
Weronika Wituszyńska (2013)
Reconstitution and structure of a plant NLR resistosome conferring immunity
Jizong Wang (2019)
Analysis of the N gene hypersensitive response induced by a fluorescently tagged tobacco mosaic virus.
K. Wright (2000)
The voltage-dependent anion channel (VDAC): function in intracellular signalling, cell life and cell death.
V. Shoshan-Barmatz (2006)
Tobacco rattle virus-A pathogen Based Vector System as a Tool for Functional Genomics
Sahil Mehta (2018)
Mitochondrion and Chloroplast Regulation of Plant Programmed Cell Death
Theresa J. Reape (2015)
Functional characterization of calcium-dependent protein kinase (CPK) 2 gene from oilseed rape (Brassica napus L.) in regulating reactive oxygen species signaling and cell death control.
Weihuan Wang (2018)
Reassessing apoptosis in plants
M. Dickman (2017)
Arabidopsis AtSPL 14 , a plant-specific SBP-domain transcription factor , participates in plant development and sensitivity to fumonisin B 1
Julie M Stone (2017)
Salicylic acid (SA)-dependent gene activation can be uncoupled from cell death-mediated gene activation: the SA-inducible NIMIN-1 and NIMIN-2 promoters, unlike the PR-1a promoter, do not respond to cell death signals in tobacco.
I. Glocova (2005)
Does the plant mitochondrion integrate cellular stress and regulate programmed cell death?
A. Jones (2000)
Predicating the Effector Proteins Secreted by Puccinia triticina Through Transcriptomic Analysis and Multiple Prediction Approaches
Y. Zhang (2020)
How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection
S. Kale (2011)
Relationship between programmed cell death and the cell cycle in the tobacco BY-2 cell line
C. Orchard (2004)
A translationally controlled tumor protein negatively regulates the hypersensitive response in Nicotiana benthamiana.
Meenu Gupta (2013)
The mitochondrial permeability transition pore (PTP) - an example of multiple molecular exaptation?
A. Vianello (2012)
Identification et analyse fonctionnelle des effecteurs tardifs impliqués dans la colonisation systémique du colza par Leptosphaeria maculans
Julie Gervais (2017)
Death of a tapetum : A programme of developmental altruism.
R. Parish (2010)
Receptor-Like Kinases BAK1 and SOBIR1 Are Required for Necrotizing Activity of a Novel Group of Sclerotinia sclerotiorum Necrosis-Inducing Effectors
Shirin Seifbarghi (2020)
Dissection of Arabidopsis Bax Inhibitor-1 Suppressing Bax–, Hydrogen Peroxide–, and Salicylic Acid–Induced Cell Death Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.014613.
M. Kawai-yamada (2004)
Yeast mutants resistant to Bax lethality reveal distinct vacuolar and mitochondrial alterations
S. Belhocine (2004)
Soil-borne wheat mosaic virus (SBWMV) 37 kDa protein rescues cell-to-cell and long-distance movement of an immobile tobacco mosaic virus mutant in Nicotiana benthamiana, a non-host of SBWMV
C. Zhang (2005)
A matter of life and death - polyamine metabolism during zygotic embryogenesis of pine
J. Vuosku (2011)
Transcriptional Programming and Functional Interactions within the Phytophthora sojae RXLR Effector Repertoire[C][W][OA]
Qunqing Wang (2011)
A Global Microarray Expression Profile of Grapevine miRNAs Isolated from Cell Suspensions Pre-Treated with Apoptosis Activators
Vladimír Řepka (2012)
Redox regulation in plant programmed cell death.
M. D. de Pinto (2012)
The tomato Arp2/3 complex is required for resistance to the powdery mildew fungus Oidium neolycopersici.
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