← Back to Search
A Novel Glycosaminoglycan-binding Protein Is The Vertebrate Homologue Of The Cell Cycle Control Protein, Cdc37 (*)
N. Grammatikakis, A. Grammatikakis, M. Yoneda, Q. Yu, S. Banerjee, B. Toole
Published 1995 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Using a monoclonal antibody, IVd4, that recognizes a novel group of hyaluronan-binding proteins, we have immunoscreened a cDNA library constructed from embryonic chick heart muscle mRNA. One of the cDNAs isolated from the library encodes a 29.3-kDa protein homologous to Cdc37, an essential cell cycle regulatory factor previously characterized genetically in yeast and Drosophila; this is the first vertebrate CDC37 gene to be cloned to date. We also present evidence for the existence of a second chick isoform that is identical to the 29.3-kDa protein over the first 175 amino acids but is entirely different at the carboxyl terminus and lacks the IVd4 epitope. The avian Cdc37 binds hyaluronan, chondroitin sulfate and heparin in vitro, and both isoforms contain glycosaminoglycan-binding motifs previously described in several hyaluronan-binding proteins. These findings suggest a role for glycosaminoglycans in cell division control.
This paper references
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
M. Frohman (1988)
Cloning and primary structure of neurocan, a developmentally regulated, aggregating chondroitin sulfate proteoglycan of brain.
U. Rauch (1992)
Hyaluronic acid modulates proliferation of mouse dermal fibroblasts in culture.
M. Yoneda (1988)
Improved tools for biological sequence comparison.
W. Pearson (1988)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
R. Dale (1985)
Correlations between heparan sulfate metabolism and hepatoma growth
M. Ishihara (1989)
CD44 and its interaction with extracellular matrix.
J. Lesley (1993)
Monoclonal antibody to chick embryo hyaluronan-binding protein: changes in distribution of binding protein during early brain development.
S. Banerjee (1991)
Hyaluronate receptors: key players in growth, differentiation, migration and tumor progression.
L. Sherman (1994)
BEHAB, a new member of the proteoglycan tandem repeat family of hyaluronan-binding proteins that is restricted to the brain [published erratum appears in J Cell Biol 1997 Apr 21;137(2):521]
D. Jaworski (1994)
Chondroitin sulfate proteoglycan (PG-M-like proteoglycan) is involved in the binding of hyaluronic acid to cellular fibronectin.
M. Yamagata (1986)
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Andrew P. Feinberg (1983)
CD44: the hyaluronan receptor.
C. Underhill (1992)
Transport of heparan sulfate into the nuclei of hepatocytes.
M. Ishihara (1986)
Heparin inhibits c-fos and c-myc mRNA expression in vascular smooth muscle cells.
L. Pukac (1990)
Hyaluronan‐binding proteins in development, tissue homeostasis, and disease
C. Knudson (1993)
Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase
M. A. Simon (1991)
Multiple domains of the large fibroblast proteoglycan, versican.
D. Zimmermann (1989)
Hyaluronan-binding protein in endothelial cell morphogenesis
S. Banerjee (1992)
Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products.
J. Ferguson (1986)
Binding of hyaluronate to the surface of cultured cells
C. Underhill (1979)
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
H. Towbin (1979)
Nuclear localization of glycosaminoglycans in rat ovarian granulosa cells.
D. R. Hiscock (1994)
Post-translational protein modification and expression of ankyrin-binding site(s) in GP85 (Pgp-1/CD44) and its biosynthetic precursors during T-lymphoma membrane biosynthesis.
V. Lokeshwar (1991)
Hyaluronate‐binding protein of simian virus 40‐transformed 3T3 cells: Membrane distribution and reconstitution into lipid vesicles
G. Chi-Rosso (1987)
Interaction of cartilage proteoglycans with hyaluronic acid.
V. Hascall (1977)
Trans-repressor activity of nuclear glycosaminoglycans on Fos and Jun/AP-1 oncoprotein-mediated transcription
S. J. Busch (1992)
Effects of detergent solubilization on the hyaluronate-binding protein from membranes of simian virus 40-transformed 3T3 cells.
C. Underhill (1983)
Cell Biology of Extracellular Matrix
B. Toole (1981)
Molecular Modeling of Protein‐Glycosaminoglycan Interactions
A. Cardin (1989)
Identification of a common hyaluronan binding motif in the hyaluronan binding proteins RHAMM, CD44 and link protein.
B. Yang (1994)
A unique heparan sulfate in the nuclei of hepatocytes: structural changes with the growth state of the cells
N. Fedarko (1986)
A heparin binding protein whose expression increases during differentiation of embryonal carcinoma cells to parietal endoderm cells: cDNA cloning and sequence analysis.
T. Furukawa (1990)
Biotinylated hyaluronic acid: a new tool for probing hyaluronate-receptor interactions.
T. Pouyani (1994)
Identification of a novel heparin binding domain in RHAMM and evidence that it modifies HA mediated locomotion of ras‐transformed cells
B. Yang (1994)
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
M. Kozak (1987)
Limb Development and Regeneration
S. Bryant (1987)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
Increased hyaluronate synthesis is required for fibroblast detachment and mitosis.
M. Brecht (1986)
Immunoelectron microscopic localization of hyaluronic acid-binding region and link protein epitopes in brain
J. A. Ripellino (1989)
The role of hyaluronan‐binding protein in assembly of pericellular matrices
Q. Yu (1992)
Hyaluronate binding properties of versican.
R. LeBaron (1992)
Molecular cloning and characterization of onchocystatin, a cysteine proteinase inhibitor of Onchocerca volvulus.
S. Lustigman (1992)
Localization and characterization of a heparin binding domain peptide of human von Willebrand factor.
M. Sobel (1992)
Isolation of a large aggregating proteoglycan from human brain.
G. Perides (1992)
Requirement of heparan sulfate for bFGF-mediated fibroblast growth and myoblast differentiation
A. Rapraeger (1991)
Expression of p60v-src in Saccharomyces cerevisiae results in elevation of p34CDC28 kinase activity and release of the dependence of DNA replication on mitosis.
F. Boschelli (1993)
An analysis of vertebrate mRNA sequences: intimations of translational control
M. Kozak (1991)
Control of cell division in hepatoma cells by exogenous heparan sulfate proteoglycan
N. Fedarko (1989)
A comparison of three heparin-binding serine proteinase inhibitors.
C. W. Pratt (1992)
Mutations in Hsp83 and cdc37 impair signaling by the sevenless receptor tyrosine kinase in Drosophila
T. Cutforth (1994)
The selection of S. cerevisiae mutants defective in the start event of cell division.
S. I. Reed (1980)
Antibody‐induced activation of the hyaluronan receptor function of CD44 requires multivalent binding by antibody
J. Lesley (1993)
Light and electron microscopic studies on the localization of hyaluronic acid in developing rat cerebellum
J. A. Ripellino (1988)
The tandemly repeated sequences of cartilage link protein contain the sites for interaction with hyaluronic acid
P. Goetinck (1987)
Proteoglycans and Hyaluronan in Morphogenesis and Differentiation
B. Toole (1991)
Isolation and identification of glycosaminoglycans associated with purified nuclei from rat liver.
K. Furukawa (1977)
This paper is referenced by
Cdc37: Implications in Regulation of Kinases and Proteins Linked to Neurodegenerative and Other Diseases
Malathi Narayan (2016)
Seeing Cell Biology with the Eyes of Physics
C. Ventura (2017)
Regulated expression of keratan sulphate and peanut agglutinin binding sites during organogenesis in the developing chick
F. Hemming (1998)
A Role for HAPLN1 During Phenotypic Modulation of Human Lung Fibroblasts In Vitro
S. Evanko (2020)
Identification of a nuclear variant of MGEA5, a cytoplasmic hyaluronidase and a beta-N-acetylglucosaminidase.
N. Comtesse (2001)
Aktivierung von humanen glatten Gefäßmuskelzellen durch Hyaluronsäure-Synthase 3: Bedeutung für die Pathogenese der Atherosklerose
B. Rabausch (2008)
Organization of the Chick CDC37 Gene*
L. Huang (1998)
Signaling Properties of Hyaluronan Receptors*
E. Turley (2002)
A model for the cytoplasmic trafficking of signalling proteins involving the hsp90-binding immunophilins and p50cdc37.
W. Pratt (1999)
Functional analysis of S.pombe Cdc37
E. Turnbull (2005)
Glypican and Biglycan in the Nuclei of Neurons and Glioma Cells: Presence of Functional Nuclear Localization Signals and Dynamic Changes in Glypican During the Cell Cycle
Y. Liang (1997)
THE PRESENCE AND PROCESSING OF INTRACELLULAR HYALURONAN IN PROLIFERATING CELLS
S. Evanko (2002)
Visualisation of hyaluronan and hyaluronan-binding proteins within ovine vertebral cartilages using biotinylated aggrecan G1-link complex and biotinylated hyaluronan oligosaccharides
J. Melrose (2002)
Glycoconjugate Profiles of Insect Spermatogenesis: Lectin Cytochemical Analysis of the Cricket, Gryllus bimaculatus
A. Suzuki (1997)
CD44-mediated uptake and degradation of hyaluronan.
W. Knudson (2002)
Golgi localization and dynamics of hyaluronan binding protein 1 (HABP1/p32/C1QBP) during the cell cycle
A. Sengupta (2005)
Physical Interaction of Mammalian CDC37 with CDK4*
K. Dai (1996)
Hyaluronan Bound to CD44 on Keratinocytes Is Displaced by Hyaluronan Decasaccharides and Not Hexasaccharides*
R. Tammi (1998)
EXAMINATION OF EXTRACELLULAR MATRIX COMPONENTS DURING HUMAN TOOTH DEVELOPMENT
S. Felszeghy (2006)
Hsp90 Regulates p50 cdc37 Function during the Biogenesis of the Active Conformation of the Heme-regulated eIF2α Kinase*
J. Shao (2001)
Lactate stimulates fibroblast expression of hyaluronan and CD44: the Warburg effect revisited.
R. Stern (2002)
Hyaluronan: from extracellular glue to pericellular cue
B. Toole (2004)
The endothelial glycocalyx: composition, functions, and visualization
S. Reitsma (2007)
Hyaluronan and the Aggregating Proteoglycans
T. Wight (2011)
p50 cdc37 Binds Directly to the Catalytic Domain of Raf as Well as to a Site on hsp90 That Is Topologically Adjacent to the Tetratricopeptide Repeat Binding Site*
A. Silverstein (1998)
MINI REVIEW Hyaluronan promotes the malignant phenotype
B. Toole (2002)
REAC technology and hyaluron synthase 2, an interesting network to slow down stem cell senescence
M. Maioli (2016)
CD44: a validated target for improved delivery of cancer therapeutics
S. Ghosh (2012)
Development and validation of H11B2C2 monoclonal antibody-reactive hyaluronic acid binding protein: overexpression of HABP during human tumor progression
Rajeev K Boregowda (2012)
Silencing of hyaluronan synthase 2 suppresses the malignant phenotype of invasive breast cancer cells
Y. Li (2007)
Hyaluronan-binding Proteins: Tying Up the Giant*
A. J. Day (2002)
Hyaluronan fragments: an information-rich system.
R. Stern (2006)See more