Online citations, reference lists, and bibliographies.
← Back to Search

Sphingosine 1-Phosphate And Platelet-derived Growth Factor (PDGF) Act Via PDGFβ Receptor-Sphingosine 1-Phosphate Receptor Complexes In Airway Smooth Muscle Cells*

C. Waters, B. Sambi, K. Kong, D. Thompson, S. Pitson, S. Pyne, N. Pyne
Published 2003 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Platelet-derived growth factor (PDGF) and sphingosine 1-phosphate (S1P) act via PDGFβ receptor-S1P1 receptor complexes in airway smooth muscle cells to promote mitogenic signaling. Several lines of evidence support this conclusion. First, both receptors were co-immunoprecipitated from cell lysates with specific anti-S1P1 antibodies, indicating that they form a complex. Second, treatment of airway smooth muscle cells with PDGF stimulated the phosphorylation of p42/p44 MAPK, and this phosphorylated p42/p44 MAPK associates with the PDGFβ receptor-S1P1 receptor complex. Third, treatment of cells with antisense S1P1receptor plasmid construct reduced the PDGF- and S1P-dependent activation of p42/p44 MAPK. Fourth, S1P and/or PDGF induced the formation of endocytic vesicles containing both PDGFβ receptors and S1P1 receptors, which was required for activation of the p42/p44 MAPK pathway. PDGF does not induce the release of S1P, suggesting the absence of a sequential mechanism. However, sphingosine kinase 1 is constitutively exported from cells and supports activation of p42/p44 MAPK by exogenous sphingosine. Thus, the presentation of sphingosine from other cell types and its conversion to S1P by the kinase exported from airway smooth muscle cells might enable S1P to act with PDGF on the PDGFβ receptor-S1P1 receptor complex to induce biological responses in vivo. These data provide further evidence for a novel mechanism for G-protein-coupled receptor and receptor tyrosine kinase signal integration that is distinct from the transactivation of receptor tyrosine kinases by G-protein-coupled receptor agonists and/or sequential release and action of S1P in response to PDGF.
This paper references
10.1016/s0021-9258(19)38849-0
An abundant transcript induced in differentiating human endothelial cells encodes a polypeptide with structural similarities to G-protein-coupled receptors.
T. Hla (1990)
10.1016/0006-2952(93)90132-G
Bradykinin stimulates phospholipase D in primary cultures of guinea-pig tracheal smooth muscle.
S. Pyne (1993)
10.1006/BBRC.1993.1163
Molecular cloning of a novel putative G protein-coupled receptor expressed in the cardiovascular system.
H. Okazaki (1993)
10.1074/JBC.270.28.16495
Gβγ Subunits Mediate Mitogen-activated Protein Kinase Activation by the Tyrosine Kinase Insulin-like Growth Factor 1 Receptor (*)
L. Luttrell (1995)
10.1074/JBC.270.42.24631
Gα12 and Gα13 Stimulate Rho-dependent Stress Fiber Formation and Focal Adhesion Assembly (*)
A. Buhl (1995)
10.1074/JBC.271.19.11272
The Inducible G Protein-coupled Receptor edg-1 Signals via the G/Mitogen-activated Protein Kinase Pathway (*)
Menq-Jer Lee (1996)
10.1126/SCIENCE.277.5323.242
Pericyte loss and microaneurysm formation in PDGF-B-deficient mice.
P. Lindahl (1997)
10.1006/GENO.1998.5491
EDG6, a novel G-protein-coupled receptor related to receptors for bioactive lysophospholipids, is specifically expressed in lymphoid tissue.
M. Gräler (1998)
10.1126/SCIENCE.279.5356.1552
Sphingosine-1-phosphate as a ligand for the G protein-coupled receptor EDG-1.
M. J. Lee (1998)
10.1074/jbc.274.34.23940
Comparison of Intrinsic Activities of the Putative Sphingosine 1-Phosphate Receptor Subtypes to Regulate Several Signaling Pathways in Their cDNA-transfected Chinese Hamster Ovary Cells*
J. Kon (1999)
10.1006/mcne.1999.0776
Molecular Cloning, Tissue-Specific Expression, and Chromosomal Localization of a Novel Nerve Growth Factor-Regulated G-Protein-Coupled Receptor, nrg-1
M. Glickman (1999)
10.1074/jbc.274.3.1185
Src-mediated Tyrosine Phosphorylation of Dynamin Is Required for β2-Adrenergic Receptor Internalization and Mitogen-activated Protein Kinase Signaling*
S. Ahn (1999)
10.1016/S0898-6568(99)00005-4
Assessment of the extracellular and intracellular actions of sphingosine 1-phosphate by using the p42/p44 mitogen-activated protein kinase cascade as a model.
D. Tolan (1999)
10.1042/0264-6021:3370171
Platelet-derived-growth-factor stimulation of the p42/p44 mitogen-activated protein kinase pathway in airway smooth muscle: role of pertussis-toxin-sensitive G-proteins, c-Src tyrosine kinases and phosphoinositide 3-kinase.
A. Conway (1999)
10.1074/jbc.274.27.18997
Differential Pharmacological Properties and Signal Transduction of the Sphingosine 1-Phosphate Receptors EDG-1, EDG-3, and EDG-5*
N. Ancellin (1999)
Role of PDGF-B and PDGFR-beta in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse.
M. Hellström (1999)
10.1042/0264-6021:3380643
Sphingosine 1-phosphate stimulation of the p42/p44 mitogen-activated protein kinase pathway in airway smooth muscle. Role of endothelial differentiation gene 1, c-Src tyrosine kinase and phosphoinositide 3-kinase.
S. Rakhit (1999)
10.1074/jbc.274.39.27351
Differential Coupling of the Sphingosine 1-Phosphate Receptors Edg-1, Edg-3, and H218/Edg-5 to the Gi, Gq, and G12 Families of Heterotrimeric G Proteins*
R. Windh (1999)
10.1074/jbc.274.8.4626
Sphingosine 1-Phosphate-induced Cell Rounding and Neurite Retraction Are Mediated by the G Protein-coupled Receptor H218*
J. V. Van Brocklyn (1999)
10.1074/JBC.275.4.2255
Association of heterotrimeric G(i) with the insulin-like growth factor-I receptor. Release of G(betagamma) subunits upon receptor activation.
H. Hallak (2000)
10.1074/jbc.M006176200
Expression of a Catalytically Inactive Sphingosine Kinase Mutant Blocks Agonist-induced Sphingosine Kinase Activation
Stuart M. Pitson (2000)
10.1074/JBC.275.19.14281
Characterization of a Novel Sphingosine 1-Phosphate Receptor, Edg-8*
D. Im (2000)
10.1074/JBC.275.13.9572
The β2-Adrenergic Receptor Mediates Extracellular Signal-regulated Kinase Activation via Assembly of a Multi-receptor Complex with the Epidermal Growth Factor Receptor*
Stuart Maudsley (2000)
10.1124/MOL.58.2.413
The platelet-derived growth factor receptor stimulation of p42/p44 mitogen-activated protein kinase in airway smooth muscle involves a G-protein-mediated tyrosine phosphorylation of Gab1.
S. Rakhit (2000)
10.1042/0264-6021:3490385
Sphingosine 1-phosphate signalling in mammalian cells
S. Pyne (2000)
10.1006/BBRC.2000.2162
Edg-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway.
Y. Yamazaki (2000)
10.1074/JBC.M102771200
Tethering of the platelet-derived growth factor beta receptor to G-protein-coupled receptors. A novel platform for integrative signaling by these receptor classes in mammalian cells.
F. Alderton (2001)
10.1124/MOL.60.1.63
Nerve growth factor stimulation of p42/p44 mitogen-activated protein kinase in PC12 cells: role of G(i/o), G protein-coupled receptor kinase 2, beta-arrestin I, and endocytic processing.
S. Rakhit (2001)
10.1126/SCIENCE.1057559
Role of the Sphingosine-1-Phosphate Receptor EDG-1 in PDGF-Induced Cell Motility
J. P. Hobson (2001)
10.1074/JBC.M102841200
Extracellular Export of Sphingosine Kinase-1 Enzyme
N. Ancellin (2002)
10.1124/pr.54.2.265
International Union of Pharmacology. XXXIV. Lysophospholipid Receptor Nomenclature
J. Chun (2002)
10.1074/jbc.M204431200
Phosphorylation of the Platelet-derived Growth Factor Receptor-β and Epidermal Growth Factor Receptor by G Protein-coupled Receptor Kinase-2
N. Freedman (2002)
10.1016/S1388-1981(02)00146-4
Sphingosine 1-phosphate signalling and termination at lipid phosphate receptors.
S. Pyne (2002)
10.1074/JBC.M110647200
Dual Regulation of EDG1/S1P1 Receptor Phosphorylation and Internalization by Protein Kinase C and G-protein-coupled Receptor Kinase 2*
K. Watterson (2002)



This paper is referenced by
10.1016/j.cellsig.2021.109929
Lipid rafts as platforms for sphingosine 1-phosphate metabolism and signalling.
Chiara D'Aprile (2021)
10.1161/CIRCULATIONAHA.119.042354
Phosphoinositide 3-Kinase–Regulated Pericyte Maturation Governs Vascular Remodeling
Ana M. Figueiredo (2020)
10.3389/fphar.2020.598007
S1PR2 Inhibition Attenuates Allergic Asthma Possibly by Regulating Autophagy
Hanye Liu (2020)
10.3389/fphar.2020.529962
Coix Seed Oil Exerts an Anti–Triple-Negative Breast Cancer Effect by Disrupting miR-205/S1PR1 Axis
Ting Fang (2020)
10.1007/s40291-019-00401-5
S1PR1 as a Novel Promising Therapeutic Target in Cancer Therapy
Narges Rostami (2019)
10.3389/fphar.2018.01369
β-Arrestin Based Receptor Signaling Paradigms: Potential Therapeutic Targets for Complex Age-Related Disorders
J. van Gastel (2018)
10.1042/BSR20181612
S1P1 receptor phosphorylation, internalization, and interaction with Rab proteins: effects of sphingosine 1-phosphate, FTY720-P, phorbol esters, and paroxetine
Juan Carlos Martínez-Morales (2018)
10.4252/wjsc.v10.i9.119
Regulatory role of sphingosine kinase and sphingosine-1-phosphate receptor signaling in progenitor/stem cells
M. L. Ng (2018)
Investigation of Intracellular Mechanisms of Sphingosine-1-Phosphate (S1P)-Induced Contractions in Detrusor Smooth Muscle of Rats having Cyclophosphamide-Induced Cystitis
Irfan Anjum (2017)
10.1016/j.cellsig.2017.03.002
Mechanisms of sphingosine 1-phosphate receptor signalling in cancer.
Sathya Narayanan Patmanathan (2017)
10.3390/molecules22030344
Sphingosine 1-Phosphate Receptor 1 Signaling in Mammalian Cells
N. Pyne (2017)
10.1152/ajpcell.00059.2016
PDGF induces SphK1 expression via Egr-1 to promote pulmonary artery smooth muscle cell proliferation.
J. R. Sysol (2016)
Regulation of Cell Signaling Pathways PDGF induces SphK 1 expression via Egr-1 to promote pulmonary artery smooth muscle cell proliferation
J. Sysol (2016)
10.1016/j.jmb.2016.08.002
Regulation, Signaling, and Physiological Functions of G-Proteins.
Viktoriya Syrovatkina (2016)
10.1158/1078-0432.CCR-15-2618
The Sphingosine-1-Phosphate Modulator FTY720 Targets Multiple Myeloma via the CXCR4/CXCL12 Pathway
K. Beider (2016)
10.1007/978-1-4939-2196-6
Dopamine Receptor Technologies
M. Tiberi (2015)
10.1007/978-1-4939-2196-6_12
Transactivation of Receptor Tyrosine Kinases by Dopamine Receptors
Jeff S. Kruk (2015)
10.1016/j.steroids.2015.10.009
Sphingosine-1-phosphate receptor 1 transmits estrogens’ effects in endothelial cells
O. Sukocheva (2015)
10.1165/rcmb.2014-0294TR
Platelet-derived growth factor signaling in the lung. From lung development and disease to clinical studies.
Nina Noskovičová (2015)
10.1007/s00210-015-1159-5
FTY720 and two novel butterfly derivatives exert a general anti-inflammatory potential by reducing immune cell adhesion to endothelial cells through activation of S1P3 and phosphoinositide 3-kinase
Faik Imeri (2015)
10.3389/fphar.2015.00040
Modulation of cellular signaling by herpesvirus-encoded G protein-coupled receptors
S. D. de Munnik (2015)
10.3390/ijms151119700
Cell-Surface Receptors Transactivation Mediated by G Protein-Coupled Receptors
F. Cattaneo (2014)
10.1164/rccm.201401-0121OC
The sphingosine kinase 1/sphingosine-1-phosphate pathway in pulmonary arterial hypertension.
J. Chen (2014)
10.1016/j.scr.2013.08.013
Sphingosine-1-phosphate-induced Flk-1 transactivation stimulates mouse embryonic stem cell proliferation through S1P1/S1P3-dependent β-arrestin/c-Src pathways.
J. Ryu (2014)
10.1016/j.cellsig.2012.09.021
5-HT(1A) receptors transactivate the platelet-derived growth factor receptor type beta in neuronal cells.
Jeff S. Kruk (2013)
10.1002/9781118531426.CH14
Sphingosine 1‐Phosphate (S1P) Signaling in Cardiovascular Physiology and Disease
B. Levkau (2013)
10.1016/j.bbalip.2012.07.022
Sphingosine-1-phosphate: a Janus-faced mediator of fibrotic diseases.
S. Schwalm (2013)
10.1371/journal.pone.0073426
Functional Cross Talk between CXCR4 and PDGFR on Glioblastoma Cells Is Essential for Migration
M. Sciaccaluga (2013)
10.3390/biom3020303
Sphingosine 1-Phosphate and Cancer: Lessons from Thyroid Cancer Cells
K. Törnquist (2013)
10.1002/9781118531426.CH9
Sphingosine Kinases: Biochemistry, Regulation, and Roles
M. Pitman (2013)
10.2147/RRBC.S28430
A novel G-protein-coupled receptor-signaling platform and its targeted translation in human disease
Samar Abdulkhalek (2013)
10.1007/978-3-7091-1511-4_8
Cardiovascular effects of sphingosine-1-phosphate (S1P).
B. Levkau (2013)
See more
Semantic Scholar Logo Some data provided by SemanticScholar