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Ultraviolet-C Overexposure Induces Programmed Cell Death In Arabidopsis, Which Is Mediated By Caspase-like Activities And Which Can Be Suppressed By Caspase Inhibitors, P35 And Defender Against Apoptotic Death*
A. Danon, V. Rotari, A. Gordon, N. Mailhac, P. Gallois
Published 2004 · Medicine, Biology
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Plants, animals, and several branches of unicellular eukaryotes use programmed cell death (PCD) for defense or developmental mechanisms. This argues for a common ancestral apoptotic system in eukaryotes. However, at the molecular level, very few regulatory proteins or protein domains have been identified as conserved across all eukaryotic PCD forms. A very important goal is to determine which molecular components may be used in the execution of PCD in plants, which have been conserved during evolution, and which are plant-specific. Using Arabidopsis thaliana, we have shown that UV radiation can induce apoptosis-like changes at the cellular level and that a UV experimental system is relevant to the study of PCD in plants. We report here that UV induction of PCD required light and that a protease cleaving the caspase substrate Asp-Glu-Val-Asp (DEVDase activity) was induced within 30 min and peaked at 1 h. This DEVDase appears to be related to animal caspases at the biochemical level, being insensitive to broad-range cysteine protease inhibitors. In addition, caspase-1 and caspase-3 inhibitors and the pan-caspase inhibitor p35 were able to suppress DNA fragmentation and cell death. These results suggest that a YVADase activity and an inducible DEVDase activity possibly mediate DNA fragmentation during plant PCD induced by UV overexposure. We also report that At-DAD1 and At-DAD2, the two A. thaliana homologs of Defender against Apoptotic Death-1, could suppress the onset of DNA fragmentation in A. thaliana, supporting an involvement of the endoplasmic reticulum in this form of the plant PCD pathway.
This paper references
Abnormalities of developmental cell death in Dad1‐deficient mice
K. Nishii (1999)
Caspase structure, proteolytic substrates, and function during apoptotic cell death
Dw Nicholson (1999)
EDS5, an Essential Component of Salicylic Acid–Dependent Signaling for Disease Resistance in Arabidopsis, Is a Member of the MATE Transporter Family Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010376.
C. Nawrath (2002)
UV-induced Programmed Cell Death
H. Matsumura (2003)
BAX and BAK Regulation of Endoplasmic Reticulum Ca2+: A Control Point for Apoptosis
L. Scorrano (2003)
Macromolecular biophysics of the plant cell wall: Concepts and methodology
M. Jarvis (2000)
Transient and specific expression of a cysteine endopeptidase associated with autolysis during differentiation of Zinnia mesophyll cells into tracheary elements.
A. Minami (1995)
H. Stennicke (2000)
Overexpression of BiP in Tobacco Alleviates Endoplasmic Reticulum Stress
N. Leborgne-Castel (1999)
An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutant.
P. Gallois (1997)
Evidence for Agrobacterium-induced apoptosis in maize cells.
G. Hansen (2000)
Knockout of Arabidopsis accelerated-cell-death11 encoding a sphingosine transfer protein causes activation of programmed cell death and defense.
P. Brodersen (2002)
Cytochrome c release and caspase activation during menadione‐induced apoptosis in plants
Y. Sun (1999)
 Interleukin-1β converting enzyme
Nancy Athornberry (1994)
DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase.
D. Kelleher (1997)
Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β
T. Nakagawa (2000)
dad‐1, an endogenous programmed cell death suppressor in Caenorhabditis elegans and vertebrates.
A. Sugimoto (1995)
Light-Dependent Death of Maize lls1 Cells Is Mediated by Mature Chloroplasts1
J. Gray (2002)
Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.
T. Nakashima (1993)
UV‐C radiation induces apoptotic‐like changes in Arabidopsis thaliana
A. Danon (1998)
Die and let live - programmed cell death in plants.
E. Lam (1999)
Higher plants and UV-B radiation: balancing damage, repair and acclimation
M. Jansen (1998)
Caspase-like protease involvement in the control of plant cell death
Eric Lam (2004)
Molecular mechanisms of UV‐induced apoptosis
D. Kulms (2000)
Electroporation of tobacco leaf protoplasts using plasmid DNA or total genomic DNA.
P. Gallois (1995)
Deletion of Dad1 in mice induces an apoptosis‐associated embryonic death
J. L. Brewster (2000)
Programmed Cell Death in Plants.
R. Pennell (1997)
Vacuolar processing enzyme is up-regulated in the lytic vacuoles of vegetative tissues during senescence and under various stressed conditions.
T. Kinoshita (1999)
Isochorismate synthase is required to synthesize salicylic acid for plant defence
M. Wildermuth (2001)
Signal transduction in the plant immune response.
J. McDowell (2000)
A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family.
T. Makishima (2000)
Legumain Forms from Plants and Animals Differ in Their Specificity
V. Rotari (2001)
Do Plant Caspases Exist?
E. Woltering (2002)
Expression of the antiapoptotic baculovirus p35 gene in tomato blocks programmed cell death and provides broad-spectrum resistance to disease
J. E. Lincoln (2002)
Molecular characterization of two plant BI-1 homologues which suppress Bax-induced apoptosis in human 293 cells
N. Bolduc (2002)
A matrix metalloproteinase gene is expressed at the boundary of senescence and programmed cell death in cucumber.
V. Delorme (2000)
The Involvement of Cysteine Proteases and Protease Inhibitor Genes in the Regulation of Programmed Cell Death in Plants
M. Solomon (1999)
Identification of paracaspases and metacaspases: two ancient families of caspase-like proteins, one of which plays a key role in MALT lymphoma.
A. Uren (2000)
The presence and subcellular localization of caspase 3‐like proteinases in plant cells
H. Korthout (2000)
Use of the lacZ reporter gene as an internal control for GUS activity in microprojectile bombarded plant tissue
G. Hull (1996)
Evolutionally conserved plant homologue of the Bax Inhibitor‐1 (BI‐1) gene capable of suppressing Bax‐induced cell death in yeast 1
M. Kawai (1999)
Apoptotic suppression by baculovirus P35 involves cleavage by and inhibition of a virus-induced CED-3/ICE-like protease.
J. Bertin (1996)
Caspases and programmed cell death in the hypersensitive response of plants to pathogens
Olga María del Pozo (1998)
Signal transduction in response to excess light: getting out of the chloroplast.
P. Mullineaux (2002)
Multiple mediators of plant programmed cell death: Interplay of conserved cell death mechanisms and plant‐specific regulators
F. Hoeberichts (2003)
Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1 (AtBI-1)
M. Kawai-yamada (2001)
Fumonisin B1–Induced Cell Death in Arabidopsis Protoplasts Requires Jasmonate-, Ethylene-, and Salicylate-Dependent Signaling Pathways
T. Asai (2000)
AtBI-1, a plant homologue of Bax inhibitor-1, suppresses Bax-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge.
P. Sánchez (2000)
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
D. Nicholson (1995)
Inhibition of ICE family proteases by baculovirus antiapoptotic protein p35.
N. Bump (1995)
Induction and expression of seed-specific promoters in Arabidopsis embryo-defective mutants.
M. Devic (1996)
Involvement of poly(ADP‐ribose) polymerase and activation of caspase‐3‐like protease in heat shock‐induced apoptosis in tobacco suspension cells
R. H. Tian (2000)
Overexpression of Bax inhibitor suppresses the fungal elicitor-induced cell death in rice (Oryza sativa L) cells.
H. Matsumura (2003)
This paper is referenced by
Mitochondria-Associated Hexokinases Play a Role in the Control of Programmed Cell Death in Nicotiana benthamiana[W]
M. Kim (2006)
UV-B overexposure induces programmed cell death in a BY-2 tobacco cell line
D. Lytvyn (2010)
The lace plant: a novel model system to study plant proteases during developmental programmed cell death in vivo.
Christina E. N. Lord (2012)
Winter wheat cells subjected to freezing temperature undergo death process with features of programmed cell death
I. V. Lyubushkina (2013)
The Application of DVDMS as a Sensitizing Agent for Sono-/Photo-Therapy
Bingjie Mai (2020)
UV Radiation-Induced Damage at Molecular Level
Swati Sen Mandi (2016)
Caspase-like proteases regulate aluminum-induced programmed cell death in peanut
Shaochang Yao (2016)
Subcellular Localization of Arabidopsis 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase1
Pablo Leivar (2005)
Sorting out the role of reactive oxygen species during plant programmed cell death induced by ultraviolet-C overexposure
Caiji Gao (2008)
Arabidopsis Ribonucleotide Reductases Are Critical for Cell Cycle Progression, DNA Damage Repair, and Plant Development[W][OA]
C. Wang (2006)
Molecular Mechanisms Underlying Vascular Development
Jae-Hoon Jung (2008)
N-glycan production in the endoplasmic reticulum of plants.
R. J. Pattison (2009)
Identification of a new Bombyx mori nucleopolyhedrovirus and analysis of its bro gene family
Jing-bo Zhou (2012)
Identification and characterisation of Arabidopsis ER accessory proteins
Alison C. Ferguson (2012)
Mechanism and Interaction of Nanoparticle-Induced Programmed Cell Death in Plants
Fatma Yanik (2018)
Non-apoptotic programmed cell death with paraptotic-like features in bleomycin-treated plant cells is suppressed by inhibition of ATM/ATR pathways or NtE2F overexpression.
O. Smetana (2012)
Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae
Chang-Jin Park (2017)
Signaling Mechanisms Regulating Diverse Plant Cell Responses to UVB Radiation
G. Ya. Fraikin (2018)
Inhibition of cathepsin B by caspase-3 inhibitors blocks programmed cell death in Arabidopsis
Y. Ge (2016)
Hydrogen peroxide accelerated the lignification process of bamboo shoots by activating the phenylpropanoid pathway and programmed cell death in postharvest storage
D. Li (2019)
Bax Inhibitor-1, a Conserved Cell Death Suppressor, Is a Key Molecular Switch Downstream from a Variety of Biotic and Abiotic Stress Signals in Plants
N. Watanabe (2009)
Death of a tapetum : A programme of developmental altruism.
R. Parish (2010)
Native cell-death genes as candidates for developing wilt resistance in transgenic banana plants
S. Ghag (2014)
Programmed cell death: genes involved in signaling, regulation, and execution in plants and animals
Gaolathe Rantong (2015)
The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment
O. Sztatelman (2015)
ATR and MKP1 play distinct roles in response to UV-B stress in Arabidopsis.
Marina A González Besteiro (2013)
Controlled cell death, plant survival and development
Eric Lam (2004)
Proteins implicated in mediating self-incompatibility-induced alterations to the actin cytoskeleton of Papaver pollen.
N. S. Poulter (2011)
Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives
Arun Shanker and B. Venkateswarlu (2011)
DEVDase activity is induced in potato leaves during Phytophthora infestans infection.
María Belén González Fernández (2012)
Hydrogen peroxide promotes programmed cell death and salicylic acid accumulation during the induced production of sesquiterpenes in cultured cell suspensions of Aquilaria sinensis.
Juan Liu (2015)
Overexpression of Tomato Homolog of Glycolate/Glycerate Transporter Gene PLGG1/AtLrgB Leads to Reduced Chlorophyll Biosynthesis
Xia Kang (2016)See more