Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Microfluidic Accumulation Assay Probes Attachment Of Biofilm Forming Diatom Cells
Kim A. Nolte, Jana Schwarze, A. Rosenhahn
Published 2017 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Abstract Testing of fouling release (FR) technologies is of great relevance for discovery of the next generation of protective marine coatings. In this paper, an accumulation assay to test diatom interaction under laminar flow with the model organism Navicula perminuta is introduced. Using time lapse microscopy with large area sampling allows determination of the accumulation kinetics of the diatom on three model surfaces with different surface properties at different wall shear stresses. The hydrodynamic conditions within the flow cell are described and a suitable shear stress range to perform accumulation experiments is identified at which statistically significant discrimination of surfaces is possible. The observed trends compare well to published adhesion preferences of N. perminuta. Also, previously determined trends of critical wall shear stresses required for cell removal from the same set of functionalized interfaces shows consistent trends. Initial attachment mediated by extracellular polymeric substances (EPS) present outside the diatoms leads to the conclusion that the FR potential of the tested coating candidates can be deducted from dynamic accumulation experiments under well-defined hydrodynamic conditions. As well as testing new coating candidates for their FR properties, monitoring of the adhesion process under flow provides additional information on the mechanism and geometry of attachment and the population kinetics.
This paper references
Effect of Substratum Surface Chemistry and Surface Energy on Attachment of Marine Bacteria and Algal Spores
L. Ista (2004)
Combinatorial materials research applied to the development of new surface coatings V. Application of a spinning water-jet for the semi-high throughput assessment of the attachment strength of marine fouling algae
F. Cassé (2007)
Settlement and adhesion of algal cells to hexa(ethylene glycol)-containing self-assembled monolayers with systematically changed wetting properties
S. Schilp (2007)
Attachment and detachment of bacteria on surfaces with tunable and switchable wettability
L. Ista (2010)
Direct Observation of Reversible Biomolecule Switching Controlled By Electrical Stimulus
Alice Pranzetti (2014)
Mini review: Biomimetic models and bioinspired surfaces for fouling control
A. J. Scardino (2011)
A theoretical model study of the influence of fluid stresses on a cell adhering to a microchannel wall.
D. Gaver (1998)
Effect of substratum
RH Simons (2004)
Effects of coating roughness and biofouling on ship resistance and powering
M. Schultz (2007)
Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channels.
E. Young (2007)
An electrically reversible switchable surface to control and study early bacterial adhesion dynamics in real-time.
Alice Pranzetti (2013)
Use of Self-Assembled Monolayers of Different Wettabilities To Study Surface Selection and Primary Adhesion Processes of Green Algal (Enteromorpha) Zoospores
M. Callow (2000)
Comparison of Velocity Profiles for Different Flow Chamber Designs Used in Studies of Microbial Adhesion to Surfaces
D. P. Bakker (2003)
MinireviewThe first kiss: establishment and control of initial adhesion by raphid diatoms.DIATOMS
R Wetherbee (1998)
The testing and evaluation of non-toxic antifouling coatings.
G. Swain (1996)
A QUANTITATIVE METHOD TO STUDY CO-ADHESION OF MICROORGANISMS IN A PARALLEL-PLATE FLOW CHAMBER - BASIC PRINCIPLES OF THE ANALYSIS
H. Vandermei (1994)
In situ measurements
KZ Hunsucker (2016)
Settlement of Ulva zoospores on patterned fluorinated and PEGylated monolayer surfaces.
J. Finlay (2008)
Adhesion of façade coating colonisers, as mediated by physico-chemical properties
Hélène Barberousse (2007)
The lift on a small sphere in a slow shear flow
P. Saffman (1965)
Enhanced adherence of human adult endothelial cells to plasma discharge modified polyethylene terephthalate.
K. Pratt (1989)
Trends in the development of environmentally friendly fouling-resistant marine coatings.
J. Callow (2011)
Adhesion and motility of fouling diatoms on a silicone elastomer
R. Holland (2004)
Bacterial biofilms: from the Natural environment to infectious diseases
L. Hall-Stoodley (2004)
Microfluidic Assay to Quantify the Adhesion of Marine Bacteria
M. P. Arpa-Sancet (2012)
Engineered antifouling microtopographies: the role of Reynolds number in a model that predicts attachment of zoospores of Ulva and cells of Cobetia marina
Chelsea M Magin (2010)
Conditioning of self-assembled monolayers at two static immersion test sites along the east coast of Florida and its effect on early fouling development
I. Thomé (2014)
Adhesion of marine fouling organisms on hydrophilic and amphiphilic polysaccharides.
S. Bauer (2013)
Diatom adhesive mucilage contains distinct supramolecular assemblies of a single modular protein.
T. Dugdale (2006)
A turbulent channel flow apparatus for the determination of the adhesion strength of microfouling organisms
M. Schultz (2000)
Isolation and biochemical
MT Buhmann (2014)
Attachment strength is a key factor in the selection of surfaces by barnacle cyprids (Balanus amphitrite) during settlement
N. Aldred (2010)
Adsorption and Desorption
S. Morrison (1990)
Attachment of bacteria
LK Ista (1996)
Properties which influence marine fouling resistance in polymers containing silicon and fluorine
R. F. Brady (1999)
The development of foul-release coatings for seagoing vessels
C. Anderson (2003)
A quantitative method to study co-adhesion of microorganisms in a parallel plate flow chamber. II: Analysis of the kinetics of co-adhesion
R. Bos (1995)
Deposition efficiency and reversibility of bacterial adhesion under flow
J. M. Meinders (1995)
Advanced nanostructures for the control of biofouling: The FP6 EU Integrated Project AMBIO
Axel Rosenhahna (2008)
Model Organic Surfaces to Probe Marine Bacterial Adhesion Kinetics by Surface Plasmon Resonance
Alice Pranzetti (2012)
Development-Length Requirements for Fully Developed Laminar Pipe Flow of Inelastic Non-Newtonian Liquids
R. Poole (2007)
Diatom community structure on in-service cruise ship hulls
K. Hunsucker (2014)
MINIREVIEW—THE FIRST KISS: ESTABLISHMENT AND CONTROL OF INITIAL ADHESION BY RAPHID DIATOMS
R. Wetherbee (1998)
Coatings Based on Side-chain Ether-linked Poly(ethylene glycol) and Fluorocarbon Polymers for the Control of Marine Biofouling
J. Youngblood (2003)
Holographic microscopy provides new insights into the settlement of zoospores of the green alga Ulva linza on cationic oligopeptide surfaces
S. M. Vater (2015)
The biology of biofouling
PJ jcis.1995.9960. Molino (2008)
Flow conditions in the vicinity of microstructured interfaces studied by holography and implications for the assembly of artificial actin networks.
S. Weisse (2011)
Static vs dynamic settlement and adhesion of diatoms to ship hull coatings
K. Zargiel (2014)
Diatom community structure on commercially available ship hull coatings
K. Zargiel (2011)
Cell adhesion to a series of hydrophilic-hydrophobic copolymers studied with a spinning disc apparatus.
T. Horbett (1988)
A novel biofilm channel for evaluating the adhesion of diatoms to non-biocidal coatings
J. Finlay (2013)
The characterization, replication and testing of dermal denticles of Scyliorhinus canicula for physical mechanisms of biofouling prevention.
T. Sullivan (2011)
Experimental and computational analysis of a novel flow channel to assess the adhesion strength of sessile marine organisms. Interface Focus
S Dimartino (2015)
The influence of surface lubricity on the adhesion of Navicula perminuta and Ulva linza to alkanethiol self-assembled monolayers
J. Bowen (2006)
Determination of bacterial collision efficiencies in a rotating disk system
R. E. Martin (1991)
A fracture mechanical analysis of fouling release from nontoxic antifouling coatings
R. F. Brady (2001)
Quantification of the adhesion strength of fibroblast cells on ethylene glycol terminated self-assembled monolayers by a microfluidic shear force assay.
C. Christophis (2010)
Economic impact of biofouling on a naval surface ship
M. Schultz (2011)
Experimental and computational analysis of a novel flow channel to assess the adhesion strength of sessile marine organisms
S. Dimartino (2015)
Novel whole cell adhesion assays of three isolates of the fouling diatom Amphora coffeaeformis reveal diverse responses to surfaces of different wettability
Oliver M Hodson (2012)
Attachment of bacteria to model solid surfaces: oligo(ethylene glycol) surfaces inhibit bacterial attachment.
L. Ista (1996)
Impact of diatomaceous biofilms on the frictional drag of fouling-release coatings
M. Schultz (2015)
Microfluidic detachment assay to probe the adhesion strength of diatoms
M. Alles (2015)
Adhesion of bacteria and diatoms to surfaces in the sea : a review
Keith E. Cooksey (1995)
Hyperbranched fluoropolymer and linear poly(ethylene glycol) based amphiphilic crosslinked networks as efficient antifouling coatings: An insight into the surface compositions, topographies, and morphologies
C. Gudipati (2004)
Influence of substratum wettability on the strength of adhesion of human fibroblasts.
T. V. van Kooten (1992)
Evaluation of Hydrodynamic Drag on Experimental Fouling-release Surfaces, using Rotating Disks
E. Holm (2004)
Biological succession on silicone fouling‐release surfaces: Long‐term exposure tests in the harbour of ischia, Italy
A. Terlizzi (2000)
Differences between healthy hematopoietic progenitors and leukemia cells with respect to CD44 mediated rolling versus adherence behavior on hyaluronic acid coated surfaces.
M. Hanke (2014)
Adsorption and desorption of colloidal particles on glass in a parallel plate flow chamber—Influence of ionic strength and shear rate
J. M. Meinders (1994)
Three Models to Relate Detachment of Low Form Fouling at Laboratory and Ship Scale
M. Schultz (2003)
Conditioning of surfaces by macromolecules and its implication for the settlement of zoospores of the green alga Ulva linza
I. Thome (2012)
Isolation and biochemical characterization of underwater adhesives from diatoms
N. Poulsen (2014)
Adhesion Strength of Settled Spores of the Green Alga Enteromorpha
J. Finlay (2002)
The Ship Hull Fouling Penalty
R. Townsin (2003)
An New Avenue to Nonfouling Materials
S. Chen (2008)
In situ measurements of diatom adhesion to silicone-based ship hull coatings
Kelli Z Hunsucker (2015)
Development and use of a parallel-plate flow chamber for studying cellular adhesion to solid surfaces.
T. V. van Kooten (1992)
Influence of electric fields and pH on biofilm structure as related to the bioelectric effect.
P. Stoodley (1997)
The biology of biofouling diatoms and their role in the development of microbial slimes
P. Molino (2008)
REAL-TIME ENUMERATION OF ADHERING MICROORGANISMS IN A PARALLEL PLATE FLOW CELL USING AUTOMATED IMAGE-ANALYSIS
J. Sjollema (1989)
The antifouling and fouling-release performance of hyperbranched fluoropolymer (HBFP)-poly(ethylene glycol) (PEG) composite coatings evaluated by adsorption of biomacromolecules and the green fouling alga Ulva.
C. Gudipati (2005)
The role of "inert" surface chemistry in marine biofouling prevention.
A. Rosenhahn (2010)
This paper is referenced by
Fouling-Release Properties of Dendritic Polyglycerols against Marine Diatoms.
Robin Wanka (2018)
Thermoregeneration of Plastrons on Superhydrophobic Coatings for Sustained Antifouling Properties
Tomer Simovich (2020)
Microfluidic bioanalytical flow cells for biofilm studies: a review.
M. Pousti (2018)
A new method for evaluation of antifouling activity of molecules against microalgal biofilms using confocal laser scanning microscopy-microfluidic flow-cells
Tiffany Le Norcy (2019)
Hydrodynamics and surface properties influence biofilm proliferation.
M. Krsmanovic (2020)
A Novel Method to Reveal a Ureolytic Biofilm Attachment and In Situ Growth Monitoring by Electrochemical Impedance Spectroscopy
María de la Concepción Aldana Romero (2020)
Dynamic field testing of coating chemistry candidates by a rotating disk system
Kim A. Nolte (2018)
Effects of crosslink density in zwitterionic hydrogel coatings on their antifouling performance and susceptibility to silt uptake
J. Koc (2020)