Online citations, reference lists, and bibliographies.

A RAPID HILIC METHOD FOR ANALYSIS OF DIAZEPAM AND ESTAZOLAM IN HUMAN PLASMA

Q. Yuan, Zhao-Jiang Liao, F. Wang, Huilian Zhang, Y. Guo, R. Li
Published 2012 · Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
A rapid method based on hydrophilic interaction chromatography (HILIC) using UV detection was developed for analysis of diazepam and estazolam in human plasma. The method utilized a Kromasil KR100-5SIL column (250 mm × 4.6 mm I.D., 5 µm) with a mobile phase consisting of acetonitrile-sodium formate buffer (10 mM, pH = 5.5) (90:10, v/v) at a flow rate of 1.0 mL/min. The detection wavelength was 254 nm. The chromatographic run time was less than 5 min per injection. All the experiments were performed at room temperature. The isolation of the analytes from plasma samples was achieved by solid phase extraction (SPE) using Oasis HLB cartridges. The standard curve range was 25–5000 ng/mL (r2 > 0.99) for diazepam and estazolam using 0.2 mL of human plasma. The limit of quantification (LOQ) and limit of detection (LOD) for both compounds were 25 and 10 ng/mL, respectively. Recoveries of the analytes ranging from 70.7% to 88.5% with intra- and inter-day precision (RSD) less than 11% were obtained at the concentrations above the LOQ. The proposed method is rapid, simple, accurate, and its mobile phase is compatible for mass spectrometry, proving an alternative method for benzodiazepine drug monitoring in forensic and clinical toxicology as well as pharmacokinetic studies.
This paper references
10.1016/j.jchromb.2005.07.040
Determination of lorazepam in plasma from children by high-performance liquid chromatography with UV detection.
S. Muchohi (2005)
10.1002/jssc.200700630
The advantages of ESI-MS detection in conjunction with HILIC mode separations: Fundamentals and applications.
Hien P. Nguyen (2008)
10.1016/0378-4347(95)00412-2
Determination of 1,4-benzodiazepines by high-performance liquid chromatography-electrospray tandem mass spectrometry.
M. Kleinschnitz (1996)
10.1007/S11426-010-4009-9
Simultaneous determination of five anti-epilepsy drugs in human plasma using liquid chromatography-mass spectrometry
Qinhua Chen (2010)
10.1002/jssc.200890032
Hydrophilic interaction chromatography.
M. Lämmerhofer (2008)
10.1016/j.talanta.2007.11.060
An improved HPLC method for rapid quantitation of diazepam and its major metabolites in human plasma.
Mohammadreza Rouini (2008)
10.1093/JAT/25.4.250
HPLC with simultaneous UV and reductive electrochemical detection at the hanging mercury drop electrode: a highly sensitive and selective tool for the determination of benzodiazepines in forensic samples.
M. Wilhelm (2001)
10.1016/J.JCHROMB.2004.03.079
Quantitative analysis of 33 benzodiazepines, metabolites and benzodiazepine-like substances in whole blood by liquid chromatography-(tandem) mass spectrometry.
B. Smink (2004)
10.1016/j.aca.2009.10.004
A review of current trends and advances in modern bio-analytical methods: chromatography and sample preparation.
L. Nováková (2009)
10.1097/00000433-199612000-00012
Sudden death and benzodiazepines.
O. Drummer (1996)
Hydrophillic Interaction Chromatography and Its Applications in the Separation of Basic Drugs
R. P. Li (2006)
10.1016/S0378-4347(97)00537-9
Methods for the measurement of benzodiazepines in biological samples.
O. Drummer (1998)
10.1080/10826070802019574
Development and Validation of an HPLC Method for the Determination of Six 1,4‐Benzodiazepines in Pharmaceuticals and Human Biological Fluids
M. N. Uddin (2008)
10.1016/0731-7085(92)80019-J
Biopharmacological data and high-performance liquid chromatographic analysis of 1,4-benzodiazepines in biological fluids: a review.
L. A. Berrueta (1992)
10.1016/S0731-7085(99)00013-8
A modified simple and rapid reversed phase liquid chromatographic method for quantification of diazepam and nordiazepam in plasma.
J. Atta-Politou (1999)
10.1016/S0003-2670(03)00304-0
Simple method for the determination of benzodiazepines in human body fluids by high-performance liquid chromatography–mass spectrometry
X. Lee (2003)
10.1016/S0140-6736(05)61365-7
Association of road-traffic accidents with benzodiazepine use
T. MacDonald (1998)
10.1016/S0731-7085(03)00426-6
Direct injection HPLC method for the determination of selected benzodiazepines in plasma using a Hisep column.
C. Pistos (2003)
10.1016/J.JCHROMB.2003.08.026
Bioanalytical liquid chromatography tandem mass spectrometry methods on underivatized silica columns with aqueous/organic mobile phases.
W. Naidong (2003)
10.1016/S0731-7085(02)00520-4
Determination of hypnotic benzodiazepines (alprazolam, estazolam, and midazolam) and their metabolites in rat hair and plasma by reversed-phase liquid-chromatography with electrospray ionization mass spectrometry.
T. Toyo’oka (2003)
Methods of Qualitative and Quantitative Analysis for Diazepam and Chlordiazepoxide in Cases Samples
(2002)
Abuse liability of benzodiazepines.
J. Woods (1987)
10.1373/CLINCHEM.2005.065631
Liquid chromatography-tandem mass spectrometry for detection of low concentrations of 21 benzodiazepines, metabolites, and analogs in urine: method with forensic applications.
Ó. Quintela (2006)
10.1016/S0731-7085(00)00325-3
Simultaneous determination of benzodiazepines in whole blood or serum by HPLC/DAD with a semi-micro column.
A. El Mahjoub (2000)
Simple Method for the Determination of Benzodiazepines in Human Body Fluids by HighPerformance Liquid Chromatography–Mass Spectrometry
X. P. Lee (2003)
10.1016/0378-4347(96)00121-1
Simultaneous determination of twelve benzodiazepines in human serum using a new reversed-phase chromatographic column on a 2-microns porous microspherical silica gel.
E. Tanaka (1996)
10.1002/JMS.590
Bromazepam determination in human plasma by high-performance liquid chromatography coupled to tandem mass spectrometry: a highly sensitive and specific tool for bioequivalence studies.
T. L. Laurito (2004)
10.1002/JSSC.200600365
Development of a validated HPLC method for the determination of four 1,4-benzodiazepines in human biological fluids.
V. Samanidou (2007)
10.1016/S0731-7085(01)00467-8
Analysis of certain tranquilizers in biological fluids.
M. Hefnawy (2002)
10.1002/jssc.200900692
Recent advances in application of hydrophilic interaction chromatography for quantitative bioanalysis.
W. Jian (2010)
10.1016/J.CHROMA.2003.08.068
Monitoring of drugs and metabolites in whole blood by restricted-access solid-phase microextraction coupled to liquid chromatography-mass spectrometry.
M. Walles (2004)
10.1021/AC9813540
High-throughput bioanalytical LC/MS/MS determination of benzodiazepines in human urine: 1000 samples per 12 hours.
J. Zweigenbaum (1999)
10.1016/S0378-4347(01)00284-5
High-performance liquid chromatographic determination of diazepam in plasma of children with severe malaria.
S. N. Muchoh (2001)
10.1016/j.talanta.2009.06.074
Simultaneous analysis of diazepam and its metabolites in rat plasma and brain tissue by HPLC-UV and SPE.
Laura Mercolini (2009)
10.1016/J.JPBA.2004.07.020
Development of a rapid RP-HPLC method for the determination of clonazepam in human plasma.
I. Bareš (2004)
10.1016/j.jchromb.2009.05.008
Rapid and quantitative screening method for 43 benzodiazepines and their metabolites, zolpidem and zopiclone in human plasma by liquid chromatography/mass spectrometry with a small particle column.
T. Ishida (2009)
10.1016/S0731-7085(01)00461-7
Direct LC analysis of five benzodiazepines in human urine and plasma using an ADS restricted access extraction column.
W. Mullett (2001)
10.1016/S0731-7085(01)00404-6
The simultaneous determination of diazepam and its three metabolites in dog plasma by high-performance liquid chromatography with mass spectroscopy detection.
Z. Liu (2001)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar