Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Hepatic And Skeletal Muscle Mitochondrial Toxicity Of Chitosan Oligosaccharides Of Normal And Diabetic Rats

J. Teodoro, A. Gomes, A. Varela, F. Duarte, A. Rolo, C. Palmeira
Published 2016 · Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Abstract Diabetes and associated conditions are now considered a worldwide epidemic, with increasing costs and burdens with no cure yet developed. The chitin-derived glucosamine biopolymer chitosan has shown promising results when supplied to diabetic patients. However, no study has investigated the possible toxic side effects of chitosan treatments, in particular when regarding the most important bioenergetic organelle, mitochondria. As such, we aimed to understand if supplementation of chitosan to the diet of normal and diabetic rats could compromise mitochondrial function on two of the major organs involved in diabetes, obesity, and metabolic regulation, the liver and skeletal muscle. We supplemented the drinking water of normal Wistar and diabetic Goto–Kakizaki rats with 0.5% chitosan for 6 weeks. We show here that, in terms of hepatic bioenergetics, chitosan was relatively inert and had no major side effects. However, regarding skeletal muscle bioenergetics, chitosan significantly affected various bioenergetic parameters. As such, we conclude that chitosan, at the tested doses, is relatively safe for treatment of diabetic situations. Nonetheless, the potential for adverse toxicological side effects appears to be present, which might be relevant if higher doses are utilized.
This paper references
10.1152/ajpendo.00103.2012
Progression of type 2 diabetes in GK rats affects muscle and liver mitochondria differently: pronounced reduction of complex II flux is observed in liver only.
W. Jørgensen (2012)
10.1248/BPB.25.188
Antidiabetic action of low molecular weight chitosan in genetically obese diabetic KK-Ay mice.
K. Hayashi (2002)
10.1093/TOXSCI/57.1.177
Bile acids affect liver mitochondrial bioenergetics: possible relevance for cholestasis therapy.
A. Rolo (2000)
10.1073/pnas.1032913100
Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: Potential role of PGC1 and NRF1
M. Patti (2003)
Research Papers : Antibacterial Effect of Chitooligosaccharides with Different Molecular Weights Prepared Using Membrane Bioreactor
S. K. Kim (2000)
10.1002/9780470110423.CH3
Determination of the activity of succinate, NADH, choline, and alpha-glycerophosphate dehydrogenases.
T. Singer (1974)
10.1248/BPB.26.1100
Antidiabetic effects of chitosan oligosaccharides in neonatal streptozotocin-induced noninsulin-dependent diabetes mellitus in rats.
Hyean-Woo Lee (2003)
Mechanisms of disease:Molecular and metabolic mechanisms of insulin resistance and beta-cell failure in type 2 diabetes
DM Muoio (2008)
10.1016/S0076-6879(78)53021-8
Mitochondrial cytochrome c: preparation and activity of native and chemically modified cytochromes c.
D. Brautigan (1978)
10.6133/APJCN.2007.16.S1.33
Antimicrobial effect of chitooligosaccharides produced by chitosanase from Pseudomonas CUY8.
Yan Wang (2007)
10.1248/BPB.23.1458
Low molecular weight chitosan prevents the progression of low dose streptozotocin-induced slowly progressive diabetes mellitus in mice.
Y. Kondo (2000)
10.1006/TAAP.1996.8099
Benzoquinone inhibits the voltage-dependent induction of the mitochondrial permeability transition caused by redox-cycling naphthoquinones.
C. A. Palmeira (1997)
10.1016/0003-2697(87)90150-3
A very fast ion-pair reversed-phase HPLC method for the separation of the most significant nucleotides and their degradation products in human red blood cells.
V. Stocchi (1987)
10.1021/jf100662r
Chitosan reduces gluconeogenesis and increases glucose uptake in skeletal muscle in streptozotocin-induced diabetic rats.
S. Liu (2010)
10.1007/BF01868720
Membrane potential of mitochondria measured with an electrode sensitive to tetraphenyl phosphonium and relationship between proton electrochemical potential and phosphorylation potential in steady state
N. Kamo (2005)
10.1016/0003-2697(91)90546-6
Isolation of skeletal muscle mitochondria from hamsters using an ionic medium containing ethylenediaminetetraacetic acid and nagarse.
S. Bhattacharya (1991)
10.1016/j.lfs.2007.03.016
An in vitro cellular analysis of the radical scavenging efficacy of chitooligosaccharides.
Eresha Mendis (2007)
10.1038/nrm2391
Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes
A. Guilherme (2008)
10.1201/9781420038798.CH17
The Nutrient Paradox: Oxidative Stress in Pancreatic beta-Cells
L. Fridlyand (2005)
10.1271/bbb.64.965
Effect of Dietary Chitosans with Different Viscosity on Plasma Lipids and Lipid Peroxidation in Rats Fed on A Diet Enriched with Cholesterol
M. Chiang (2000)
10.1006/TAAP.1994.1138
Interactions of herbicides 2,4-D and dinoseb with liver mitochondrial bioenergetics.
C. Palmeira (1994)
10.1007/S11892-005-0006-3
Mitochondrial dysfunction and type 2 diabetes
R. Parish (2005)
Antitumor activity of chitosan oligosaccharides produced in ultrafiltration membrane reactor system
Y. Jeon (2002)
10.1080/14786410701371215
In vitro antioxidative activities of three marine oligosaccharides
P. Wang (2007)
Bioenergetics and the formation
V Adam-Vizi (2006)
10.1016/s0021-9258(18)57021-6
Determination of serum proteins by means of the biuret reaction.
A. Gornall (1949)
10.3748/WJG.V13.I5.725
Antidiabetic effects of chitooligosaccharides on pancreatic islet cells in streptozotocin-induced diabetic rats.
B. Liu (2007)
10.1016/j.diabres.2013.11.002
Global estimates of diabetes prevalence for 2013 and projections for 2035.
L. Guariguata (2014)
10.2183/PJAB1945.51.80
Spontaneous Diabetes Produced by Selective Breeding of Normal Wistar Rats
Y. Goto (1975)
Mitochondrial dysfunction
C Biochem 192344–9. Bonnard (2008)
10.1016/J.TIPS.2006.10.005
Bioenergetics and the formation of mitochondrial reactive oxygen species.
V. Ádám-Vizi (2006)
10.1016/j.fct.2007.12.012
A comparative study on hypoglycemic and hypocholesterolemic effects of high and low molecular weight chitosan in streptozotocin-induced diabetic rats.
Hsien-Tsung Yao (2008)
10.1016/j.taap.2008.08.005
Indirubin-3'-oxime impairs mitochondrial oxidative phosphorylation and prevents mitochondrial permeability transition induction.
A. Varela (2008)
10.3746/jkfn.2004.33.2.236
Antimicrobial Effect of Chitosan and Chitooligosaccharides against Bacterial Diseases of Cultured Flounder
양병규 (2004)
10.1620/TJEM.137.453
Impaired insulin secretion in the spontaneous diabetes rats.
K. Kimura (1982)
10.1016/0076-6879(67)10010-4
[7] Mitochondrial respiratory control and the polarographic measurement of ADP : O ratios
R. Estabrook (1967)
Mitochondrial respiratory control and the polarographic measurement of ADP
RW Estabrook (1967)
10.1038/ng1180
PGC-1α-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes
V. Mootha (2003)
10.1172/JCI32601
Mitochondrial dysfunction results from oxidative stress in the skeletal muscle of diet-induced insulin-resistant mice.
C. Bonnard (2008)
10.1016/J.BBAGEN.2004.11.009
Depolymerized products of chitosan as potent inhibitors of tumor-induced angiogenesis.
K. H. Harish Prashanth (2005)
10.1023/B:PHAM.0000016249.52831.a5
Uptake and Cytotoxicity of Chitosan Molecules and Nanoparticles: Effects of Molecular Weight and Degree of Deacetylation
M. Huang (2004)
10.1038/414813a
Biochemistry and molecular cell biology of diabetic complications
M. Brownlee (2001)
10.1016/j.mito.2013.09.002
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation.
J. Teodoro (2013)
10.1056/NEJMOA031314
Impaired mitochondrial activity in the insulin-resistant offspring of patients with type 2 diabetes.
K. Petersen (2004)
10.2337/DIABETES.51.10.2944
Dysfunction of mitochondria in human skeletal muscle in type 2 diabetes.
D. Kelley (2002)
10.1007/978-3-642-67530-0_6
Preparation and Assay of Animal Mitochondria and Submitochondrial Vesicles
P. Gazzotti (1979)
10.3177/JNSV.48.379
Plasma lipoprotein cholesterol in rats fed a diet enriched in chitosan and cholesterol.
Hsien-Tsung Yao (2002)
10.1016/J.TAAP.2006.01.003
Diabetes and mitochondrial function: role of hyperglycemia and oxidative stress.
A. Rolo (2006)
Potential Immuno-Stimulating Effect of Antitumoral Fraction of Chitosan Oligosaccharides
전유진 (2001)
10.1016/J.FOODCHEM.2007.08.044
Hypocholesterolaemic effects of different chitosan samples in vitro and in vivo
Jingna Liu (2008)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar