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Genotoxicity Of Engineered Nanomaterials: A Critical Review
L. Gonzalez, D. Lison, M. Kirsch-Volders
Published 2008 · Computer Science
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In view of the fast-growing industrial applications of engineered nanomaterials (ENMs), the evaluation of their genotoxic potential and of their mode of action is a necessity to conduct adequate hazard/risk assessment and to produce safer and sustainable ENMs. This review aims at: (i) Providing an evaluation of in vitro and in vivo genotoxicity data available for ENM, and (ii) proposing minimal criteria for conducting nano-genotoxicity assays. The possible modes of action of ENM (i.e., generation of reactive oxygen species (ROS) and mechanical interference with cellular components) and the potential cellular targets are discussed. The available studies are evaluated on the basis of specific quality criteria after categorisation according to ENMs type/size investigated. No definitive conclusion can be drawn concerning the genotoxic activity of ENMs, essentially because of the limited number of data, incomplete physico-chemical characterization of ENMs examined and shortcomings in experimental approaches. This evaluation revealed gaps to be considered in future studies (e.g., one-sided approach focusing mainly on ROS as mode of action) and the need to develop adequate positive controls for genotoxicity assays when conducted with nanomaterials.
This paper references
Toxicity of luminescent silica nanoparticles to living cells.
Y. Jin (2007)
Physicochemical mechanism of the interaction between cobalt metal and carbide particles to generate toxic activated oxygen species.
D. Lison (1995)
Oxyradicals and DNA damage.
L. Marnett (2000)
Indication for thresholds of chromosome non-disjunction versus chromosome lagging induced by spindle inhibitors in vitro in human lymphocytes.
A. Elhajouji (1997)
Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells.
Jia-Ran Gurr (2005)
Chromosome nondisjunction yields tetraploid rather than aneuploid cells in human cell lines
Q. Shi (2005)
An investigation of the photo-clastogenic potential of ultrafine titanium dioxide particles.
Elizabeth Theogaraj (2007)
Nuclear pore complex is able to transport macromolecules with diameters of about 39 nm.
N. Pante (2002)
Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique.
R. Murdock (2008)
Interference by toxic metal compounds with isolated zinc finger DNA repair proteins.
M. Asmuss (2000)
Cytokinesis-block micronucleus cytome assay
M. Fenech (2007)
Oops they did it again! Carbon nanotubes hoax scientists in viability assays.
J. M. Wörle-Knirsch (2006)
Impact of Types of Lymphocyte Chromosomal Aberrations on Human Cancer Risk
L. Hagmar (2004)
Particulate-state carcinogenesis: a survey of recent studies on the mechanisms of action of fibres.
M. Jaurand (1989)
Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing
R. Tice (2000)
DNA damage induced by multiwalled carbon nanotubes in mouse embryonic stem cells.
Lin Zhu (2007)
Chronic inorganic arsenic exposure induces hepatic global and individual gene hypomethylation: implications for arsenic hepatocarcinogenesis.
H. Chen (2004)
Genetic susceptibility of newborn daughters to oxidative stress.
I. Decordier (2007)
Cytotoxicity and genotoxicity of ultrafine crystalline SiO2 particulate in cultured human lymphoblastoid cells
J. Wang (2007)
Free radicals, metals and antioxidants in oxidative stress-induced cancer.
M. Valko (2006)
HIF-1, An Oxygen and Metal Responsive Transcription Factor
P. Maxwell (2004)
MAGNETOLIPOSOME EVALUATION USING CYTOMETRY AND MICRONUCLEUS TEST
L. Lacava (2002)
In vivo genotoxicity of hard metal dust: induction of micronuclei in rat type II epithelial lung cells.
M. De Boeck (2003)
Evaluation of the in vitro direct and indirect genotoxic effects of cobalt compounds using the alkaline comet assay. Influence of interdonor and interexperimental variability.
M. De Boeck (1998)
Increased inflammation and intracellular calcium caused by ultrafine carbon black is independent of transition metals or other soluble components
D. Brown (2000)
Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells.
J. Wang (2007)
Direct interaction between crystalline silica and DNA - a proposed model for silica carcinogenesis.
L. Daniel (1995)
Mutagenicity of water-soluble ZnO nanoparticles in Ames test.
R. Yoshida (2009)
Effects of particle exposure and particle-elicited inflammatory cells on mutation in rat alveolar epithelial cells.
K. Driscoll (1997)
Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis.
B. Fubini (2003)
Soluble metals as well as the insoluble particle fraction are involved in cellular DNA damage induced by particulate matter
A. Knaapen (2004)
In vitro mutagenicity and genotoxicity study of a number of short-chain chlorinated hydrocarbons using the micronucleus test and the alkaline single cell gel electrophoresis technique (Comet assay) in human lymphocytes: a structure-activity relationship (QSAR) analysis of the genotoxic and cytotoxic
M. Tafazoli (1998)
Reactivity of carbon nanotubes: free radical generation or scavenging activity?
I. Fenoglio (2006)
The murine DNA glycosylase NEIL2 (mNEIL2) and human DNA polymerase beta bind microtubules in situ and in vitro.
K. Conlon (2005)
Dynamics of Endocytosis and Exocytosis of Poly(D,L-Lactide-co-Glycolide) Nanoparticles in Vascular Smooth Muscle Cells
J. Panyam (2004)
Mitotic checkpoints and the maintenance of the chromosome karyotype.
I. Decordier (2008)
Rapid endo‐lysosomal escape of poly(DL‐lactide‐coglycolide) nanoparticles: implications for drug and gene delivery
J. Panyam (2002)
Size-dependent proinflammatory effects of ultrafine polystyrene particles: a role for surface area and oxidative stress in the enhanced activity of ultrafines.
D. Brown (2001)
Challenges for assessing carbon nanomaterial toxicity to the skin
N. Monteiro-Riviere (2006)
Formation of nucleoplasmic protein aggregates impairs nuclear function in response to SiO2 nanoparticles.
Min Chen (2005)
Mutagenicity and repair of oxidative DNA damage: insights from studies using defined lesions.
D. Wang (1998)
Inclusion of micronuclei in non-divided mononuclear lymphocytes and necrosis/apoptosis may provide a more comprehensive cytokinesis block micronucleus assay for biomonitoring purposes.
M. Kirsch-Volders (2001)
Inhaled particles and lung cancer. Part A: Mechanisms
A. Knaapen (2004)
Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy
G. Oberdörster (2005)
Role of oxygen radicals in DNA damage and cancer incidence
M. Valko (2004)
IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety.
R. Albertini (2000)
Metallothionein: the multipurpose protein
P. Coyle (2002)
Mutagenicity of water-soluble FePt nanoparticles in Ames test.
S. Maenosono (2007)
Ultrafine Particles Cross Cellular Membranes by Nonphagocytic Mechanisms in Lungs and in Cultured Cells
M. Geiser (2005)
Cobalt(II) inhibits the incision and the polymerization step of nucleotide excision repair in human fibroblasts.
U. Kasten (1997)
Indications for a threshold of chemically‐induced aneuploidy in vitro in human lymphocytes
A. Elhajouji (1995)
Comparison of the peripheral blood micronucleus test using flow cytometry in rat and mouse exposed to aneugens after single-dose applications.
Zoryana Cammerer (2007)
In vitro mutagenicity and genotoxicity study of 1,2-dichloroethylene, 1,1,2-trichloroethane, 1,3-dichloropropane, 1,2,3-trichloropropane and 1,1,3-trichloropropene, using the micronucleus test and the alkaline single cell gel electrophoresis technique (comet assay) in human lymphocytes.
M. Tafazoli (1996)
Forum series: research strategies for safety evaluation of nanomaterials.
M. Holsapple (2005)
Arsenite inhibits mitotic division and perturbs spindle dynamics in HeLa S3 cells.
S. Huang (1998)
How meaningful are the results of nanotoxicity studies in the absence of adequate material characterization?
D. Warheit (2008)
DNA hypermethylation of promoter of gene p53 and p16 in arsenic-exposed people with and without malignancy.
Sarmishtha Chanda (2006)
Ultrafine particles cause cytoskeletal dysfunctions in macrophages: role of intracellular calcium
W. Moeller (2005)
Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of muta™mouse lung epithelial cells
N. R. Jacobsen (2007)
Nanoparticle-driven DNA damage mimics irradiation-related carcinogenesis pathways
R. Mróz (2008)
IPCS guidelines for the monitoring
Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm.
T. Xia (2006)
Uptake of FITC-Chitosan Nanoparticles by A549 Cells
M. Huang (2004)
Centrosome amplification and multinuclear phenotypes are Induced by hydrogen peroxide
Sunyoung Chae (2005)
In vivo micronucleus test with flow cytometry after acute and chronic exposures of rats to chemicals.
Zoryana Cammerer (2007)
Cytotoxicity of nanoparticles.
Nastassja Lewinski (2008)
Genotoxicity and inflammatory investigation in mice treated with magnetite nanoparticles surface coated with polyaspartic acid
N. Sadeghiani (2005)
Exposure of engineered nanoparticles to human lung epithelial cells: influence of chemical composition and catalytic activity on oxidative stress.
Ludwig K. Limbach (2007)
In vitro genotoxicity of PAH mixtures and organic extract from urban air particles part II: human cell lines.
O. Sevastyanova (2007)
Spectroscopic analysis confirms the interactions between single walled carbon nanotubes and various dyes commonly used to assess cytotoxicity
A. Casey (2007)
Toxicity and tissue distribution of magnetic nanoparticles in mice.
J. Kim (2006)
Initial in vitro screening approach to investigate the potential health and environmental hazards of Envirox™ – a nanoparticulate cerium oxide diesel fuel additive
B. Park (2007)
Genotoxicity in cell lines induced by chronic exposure to water-soluble fullerenes using micronucleus test
Y. Niwa (2006)
In vitro interactions between DMSA-coated maghemite nanoparticles and human fibroblasts: A physicochemical and cytogenotoxical study
M Auffan (2006)
Cell cycle regulation of the murine 8-oxoguanine DNA glycosylase (mOGG1): mOGG1 associates with microtubules during interphase and mitosis.
K. Conlon (2004)
Indirect mechanisms of genotoxicity.
M. Kirsch-Volders (2003)
Differential effects of toxic metal compounds on the activities of Fpg and XPA, two zinc finger proteins involved in DNA repair.
M. Asmuss (2000)
Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells
J. Kim (2006)
Genotoxicity of Poorly Soluble Particles
R. Schins (2007)
The effect of nano- and micron-sized particles of cobalt-chromium alloy on human fibroblasts in vitro.
I. Papageorgiou (2007)
The in vitro micronucleus technique.
M. Fenech (2000)
Hydroxyl radicals (*OH) are associated with titanium dioxide (TiO(2)) nanoparticle-induced cytotoxicity and oxidative DNA damage in fish cells.
J. F. Reeves (2008)
Importance of detecting numerical versus structural chromosome aberrations.
M. Kirsch-Volders (2002)
Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts.
Q. Rahman (2002)
Investigations on the inflammatory and genotoxic lung effects of two types of titanium dioxide: untreated and surface treated.
B. Rehn (2003)
The reliability and limits of the MTT reduction assay for carbon nanotubes-cell interaction
L. Belyanskaya (2007)
Concepts of threshold in mutagenesis and carcinogenesis.
M. Kirsch-Volders (2000)
Very low concentrations of arsenite suppress poly(ADP‐ribosyl)ation in mammalian cells
A. Hartwig (2003)
Development of a base set of toxicity tests using ultrafine TiO2 particles as a component of nanoparticle risk management.
D. Warheit (2007)
In vitro interactions between DMSA-coated maghemite nanoparticles and human fibroblasts: A physicochemical and cyto-genotoxical study.
M. Auffan (2006)
Chemical characteristics and mutagenic activity of PM10 in Torino, a northern Italian city.
G. Gilli (2007)
Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles
G. Oberdörster (2005)
Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants.
K. Pulskamp (2007)
Nonfunctionalized nanocrystals can exploit a cell's active transport machinery delivering them to specific nuclear and cytoplasmic compartments.
I. Nabiev (2007)
MECHANISMS OF GENOTOXICITY OF PARTICLES AND FIBERS
R. Schins (2002)
Targeting of nanoparticles to the clathrin-mediated endocytic pathway.
Oshrat Harush-Frenkel (2007)
Inhaled particles and lung cancer
AM Knaapen (2004)
Role of the alveolar macrophage in lung injury: studies with ultrafine particles.
G. Oberdörster (1992)
Clastogenic and aneugenic effects of multi-wall carbon nanotubes in epithelial cells.
J. Muller (2008)
This paper is referenced by
Interactions with the Human Body
R. Colognato (2012)
Enzyme-Like Activity of Nanomaterials
W. He (2014)
Caenorhabditis elegans: Evaluation of Nanoparticle Toxicity
S. Kumar (2020)
Nanotechnology for Agriculture: Crop Production & Protection
Deepak G. Panpatte (2019)
Effets des nanoparticules de dioxyde de titane sur les bactéries : de la cellule à la communauté
Stéphane Jomini (2014)
In vitro genotoxicity assessment of nickel(II) oxide nanoparticles on lymphocytes of human peripheral blood
Naresh Dumala (2019)
Toxicity of TiO2, in nanoparticle or bulk form to freshwater and marine microalgae under visible light and UV-A radiation.
M. Sendra (2017)
Focal amplification of HOXD-harboring chromosome region is implicated in multiple-walled carbon nanotubes-induced carcinogenicity.
Ping Wu (2013)
Effects of TiO2 nanoparticles and sunscreens on coastal marine microalgae: Ultraviolet radiation is key variable for toxicity assessment.
M. Sendra (2017)
Genotoxicity of tungsten carbide-cobalt (WC-Co) nanoparticles in vitro: mechanisms-of-action studies.
H. Moche (2015)
Proposed minimum characterization parameters for studies on food and food-related nanomaterials.
J. W. Card (2009)
A Method to Assess the Quality of Studies That Examine the Toxicity of Engineered Nanomaterials
J. W. Card (2010)
Silver nanoparticles biosynthesized by Anabaena flos-aquae enhance the apoptosis in breast cancer cell line
Z. Ebrahimzadeh (2020)
Sunscreens containing zinc oxide nanoparticles can trigger oxidative stress and toxicity to the marine copepod Tigriopus japonicus.
Stella W Y Wong (2020)
Carbon nanotubes: an insight into the mechanisms of their potential genotoxicity.
D. van Berlo (2012)
An appraisal of the published literature on the safety and toxicity of food-related nanomaterials
J. W. Card (2011)
Raman Spectroscopy and Hyperspectral Analysis of Living Cells Exposed to Nanoparticles
Linnéa Ahlinder (2015)
A novel CuFe2O4@Ag nanocomposite biosynthesized by Spirulina platensis exhibits an anticancer effect on human gastric adenocarcinoma and Michigan Cancer Foundation‐7 breast cancer cell lines
Mohammad Garfami (2020)
Progressive stress response of the anaerobic granular sludge to nickel nanoparticles: experimental investigations and mathematic modelling
Chuan-Shu He (2019)
Irradiation-Enhanced Cytotoxicity of Zinc Oxide Nanoparticles
Qingbo Yang (2014)
Nanoparticle Toxicity and Environmental Impact
Y. Mo (2016)
Polymorph- and size-dependent uptake and toxicity of TiO₂ nanoparticles in living lung epithelial cells.
P. Andersson (2011)
Nanoparticles effects on growth and differentiation in cell culture of carrot (Daucus carota L.) (Conference Paper)
L. Giorgetti (2011)
FDA’s Regulation of Nanotechnology in Food Ingredients
T. Croce (2014)
The nanosilica hazard: another variable entity
D. Napierska (2010)
European Centre for Ecotoxicology and Toxicology of Chemicals
N. Carmichael (2014)
Investigating the hazards posed by pristine and modified copper nanomaterials using in vitro screens combined with in vivo models
D. Pantano (2018)
Current investigations into the genotoxicity of zinc oxide and silica nanoparticles in mammalian models in vitro and in vivo: carcinogenic/genotoxic potential, relevant mechanisms and biomarkers, artifacts, and limitations
J. Y. Kwon (2014)
Common Gene Expression Patterns in Environmental Model Organisms Exposed to Engineered Nanomaterials: A Meta-Analysis
M. Burkard (2019)
Mechanism of Action of Nanopesticide Derived from Microorganism for the Alleviation of Abiotic and Biotic Stress Affecting Crop Productivity
Charles Oluwaseun Adetunji (2019)
Advances in the Analysis of Challenging Food Contaminants: Nanoparticles, Bisphenols, Mycotoxins, and Brominated Flame Retardants
Lubinda Mbundi (2014)
Nanoparticles and Human Health: A Review of Epidemiological Studies
V. Sutariya (2014)See more