Online citations, reference lists, and bibliographies.
← Back to Search

A Nanocarrier System That Potentiates The Effect Of Miconazole Within Different Interkingdom Biofilms

L. Arias, J. Brown, M. Butcher, C. Delaney, D. Monteiro, G. Ramage
Published 2020 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
ABSTRACT Background Novel and new therapeutic strategies capable of enhancing the efficacy of existing antimicrobials is an attractive proposition to meet the needs of society. Objective This study aimed to evaluate the potentiating effect of a miconazole (MCZ) nanocarrier system, incorporated with iron oxide nanoparticles (IONPs) and chitosan (CS) (IONPs-CS-MCZ). This was tested on three representative complex interkingdom oral biofilm models (caries, denture and gingivitis). Materials and methods The planktonic and sessile minimum inhibitory concentrations (MICs) of IONPs-CS-MCZ against different Candida albicans strains were determined, as well as against all represented bacterial species that formed within the three biofilm models. Biofilms were treated for 24 hours with the IONPs-CS nanocarrier system containing MCZ at 64 mg/L, and characterized using a range of bioassays for quantitative and qualitative assessment. Results MIC results generally showed that IONPs-CS-MCZ was more effective than MCZ alone. IONPs-CS-MCZ also promoted reductions in the number of CFUs, biomass and metabolic activity of the representative biofilms, as well as altering biofilm ultrastructure when compared to untreated biofilms. IONPs-CS-MCZ affected the composition and reduced the CFEs for most of the microorganisms present in the three evaluated biofilms. In particular, the proportion of streptococci in the biofilm composition were reduced in all three models, whilst Fusobacterium spp. percentage reduced in the gingivitis and caries models, respectively. Conclusion In conclusion, the IONPs-CS-MCZ nanocarrier was efficient against three in vitro models of pathogenic oral biofilms, showing potential to possibly interfere in the synergistic interactions among fungal and bacterial cells within polymicrobial consortia.
This paper references
Novel nanocarrier of miconazole based on chitosan-coated iron oxide nanoparticles as a nanotherapy to fight Candida biofilms.
L. S. Arias (2020)
Antibiofilm effect of chlorhexidine-carrier nanosystem based on iron oxide magnetic nanoparticles and chitosan.
Ana Paula Miranda Vieira (2019)
Candida-Bacteria Interactions: Their Impact on Human Disease.
Devon L Allison (2016)
Azole cross-resistance to ketoconazole, fluconazole, itraconazole and voriconazole in clinical Candida albicans isolates from HIV-infected children with oropharyngeal candidosis.
F. Müller (2000)
Evolution of high-level resistance during low-level antibiotic exposure
Erik Wistrand-Yuen (2018)
Antibacterial and antifungal activity of chitosan coated iron oxide nanoparticles
P. Nehra (2018)
Interkingdom networking within the oral microbiome.
A. Nobbs (2015)
Cellular Components Mediating Coadherence of Candida albicans and Fusobacterium nucleatum
T. Wu (2015)
Bacterial-derived exopolysaccharides enhance antifungal drug tolerance in a cross-kingdom oral biofilm
D. Kim (2018)
Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach
G. Ramage (2019)
Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis.
Katherine Miranda-Cadena (2018)
Candida–streptococcal interactions in biofilm-associated oral diseases
H. Koo (2018)
Global epidemiology of dental caries and severe periodontitis – a comprehensive review
J. Frencken (2017)
Oral Candida spp carriage and periodontal diseases in HIV-infected patients in Ribeirão Preto, Brazil
A. G. Lourenço (2017)
Chitosan as antimicrobial agent: applications and mode of action.
E. Rabea (2003)
Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis
L S Arias (2018)
Streptococcal co-infection augments Candida pathogenicity by amplifying the mucosal inflammatory response
H. Xu (2014)
Polymicrobial oral biofilm models: simplifying the complex.
J. L. Brown (2019)
Prophylactic treatment with miconazole in patients highly predisposed to fungal infection. A placebo-controlled double-blind study.
H. Brincker (1978)
Improved detection of infection in hip replacements. A currently underestimated problem.
M. Tunney (1998)
Candida albicans Mycofilms Support Staphylococcus aureus Colonization and Enhances Miconazole Resistance in Dual-Species Interactions
Ryan Kean (2017)
New antimicrobial therapies used against fungi present in subgingival sites--a brief review.
J. Sardi (2011)
Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells
J. L. Brown (2019)
Association between oral candidiasis and bacterial pneumonia: a retrospective study.
M. Nakajima (2019)
Evaluating Streptococcus mutans Strain Dependent Characteristics in a Polymicrobial Biofilm Community
Y. Zhou (2018)
Candida albicans Yeast, Pseudohyphal, and Hyphal Morphogenesis Differentially Affects Immune Recognition
Liliane Mukaremera (2017)
Cross-Resistance of clinical isolates of Candida albicans and Candida glabrata to over-the-counter azoles used in the treatment of vaginitis.
E. W. Cross (2000)
Innocent until proven guilty: mechanisms and roles of Streptococcus–Candida interactions in oral health and disease
H. Xu (2014)
Reactive oxygen species-inducing antifungal agents and their activity against fungal biofilms.
Nicolas Delattin (2014)
Fungal Biofilm Resistance
G. Ramage (2012)
Molecular identification and antifungal susceptibility testing of Candida species isolated from dental plaques.
N. Aslani (2018)
An In Vitro Model for Oral Mixed Biofilms of Candida albicans and Streptococcus gordonii in Synthetic Saliva
Daniel Montelongo-Jauregui (2016)
High-resolution Visualization of the Microbial Glycocalyx with Low-voltage Scanning Electron Microscopy: Dependence on Cationic Dyes
S. Erlandsen (2004)
Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates
J. Allkja (2020)
Viable Compositional Analysis of an Eleven Species Oral Polymicrobial Biofilm
L. Sherry (2016)
Antibacterial activity and mechanism of action of chitosan solutions against apricot fruit rot pathogen Burkholderia seminalis.
Miao-miao Lou (2011)
Multi-functional nanocarriers based on iron oxide nanoparticles conjugated with doxorubicin, poly(ethylene glycol) and folic acid as theranostics for cancer therapy.
S. Rajkumar (2018)
Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity
L. S. Arias (2018)
Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance
M. M. Harriott (2009)
Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?
Christopher Delaney (2018)
New insights on the antibacterial efficacy of miconazole in vitro
P. Nenoff (2017)
Current treatment of oral candidiasis: A literature review
Carla Garcia-Cuesta (2014)
Community Development between Porphyromonas gingivalis and Candida albicans Mediated by InlJ and Als3
M. Sztukowska (2018)
The estimation of the bactericidal power of the blood.
A. Miles (1938)
Nano based drug delivery systems: recent developments and future prospects
J. Patra (2018)

Semantic Scholar Logo Some data provided by SemanticScholar