Online citations, reference lists, and bibliographies.
← Back to Search

Developments In The Formulation And Delivery Of Spray Dried Vaccines

Gaurav Kanojia, R. Have, P. Soema, H. Frijlink, Jean-Pierre Amorij, G. Kersten
Published 2017 · Materials Science, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
ABSTRACT Spray drying is a promising method for the stabilization of vaccines, which are usually formulated as liquids. Usually, vaccine stability is improved by spray drying in the presence of a range of excipients. Unlike freeze drying, there is no freezing step involved, thus the damage related to this step is avoided. The edge of spray drying resides in its ability for particles to be engineered to desired requirements, which can be used in various vaccine delivery methods and routes. Although several spray dried vaccines have shown encouraging preclinical results, the number of vaccines that have been tested in clinical trials is limited, indicating a relatively new area of vaccine stabilization and delivery. This article reviews the current status of spray dried vaccine formulations and delivery methods. In particular it discusses the impact of process stresses on vaccine integrity, the application of excipients in spray drying of vaccines, process and formulation optimization strategies based on Design of Experiment approaches as well as opportunities for future application of spray dried vaccine powders for vaccine delivery.
This paper references
10.1038/nbt0109-26
Quality by design for biopharmaceuticals
A. Rathore (2009)
10.1016/S1473-3099(10)70157-2
Needle-free influenza vaccination.
Jean-Pierre Amorij (2010)
10.3109/1061186X.2010.523790
Nasal vaccine innovation
I. Jabbal‐Gill (2010)
10.1016/J.VACCINE.2004.06.049
A novel dry powder influenza vaccine and intranasal delivery technology: induction of systemic and mucosal immune responses in rats.
Juan Huang (2004)
10.1006/ABBI.1998.0948
Effects of additives on the stability of recombinant human factor XIII during freeze-drying and storage in the dried solid.
L. Kreilgaard (1998)
FDA Guideline
10.1128/CVI.00447-08
Protective Immunity in Mice Achieved with Dry Powder Formulation and Alternative Delivery of Plague F1-V Vaccine
J. Huang (2009)
Implementation of Quality by Design in Vaccine development: BioProcess international
P. Calcott (2013)
10.1016/j.tibtech.2010.12.009
New developments in dry powder pulmonary vaccine delivery.
Tomás Sou (2011)
A novel dry powder influenza
J Huang
10.1002/JPS.10296
Thermal stability of vaccines.
D. Brandau (2003)
10.1208/pt0804081
Novel dry powder preparations of whole inactivated influenza virus for nasal vaccination
Robert J. Garmise (2008)
10.1089/jamp.2014.1176
Spray-Dried Influenza Antigen with Trehalose and Leucine Produces an Aerosolizable Powder Vaccine Formulation that Induces Strong Systemic and Mucosal Immunity after Pulmonary Administration.
Tomás Sou (2015)
10.1016/J.YMETH.2006.05.016
Pathogen recognition and development of particulate vaccines: does size matter?
S. Xiang (2006)
10.1080/07373938908916598
SPRAY DRYING HANDBOOK
E. J. Crosby (1989)
10.1016/J.IJPHARM.2005.04.008
Evaluation of Eudragit-coated chitosan microparticles as an oral immune delivery system.
Mika Hori (2005)
10.1016/j.ijpharm.2013.02.057
An approach to a cold chain free oral cholera vaccine: in vitro and in vivo characterization of Vibrio cholerae gastro-resistant microparticles.
M. Pastor (2013)
and a meningitis A polysaccharide conjugate vaccine produced by a spray drying method
C Anish (2010)
10.1016/j.bbapap.2013.01.020
Unraveling protein stabilization mechanisms: vitrification and water replacement in a glass transition temperature controlled system.
N. Grasmeijer (2013)
10.1016/j.tibtech.2009.06.006
Roadmap for implementation of quality by design (QbD) for biotechnology products.
A. Rathore (2009)
10.1016/j.vaccine.2016.12.009
Stability and pre-formulation development of a plant-produced anthrax vaccine candidate.
R. Jones (2017)
10.1002/jps.23998
Next generation drying technologies for pharmaceutical applications.
Robert Henry Walters (2014)
10.1016/j.pvr.2017.03.004
Characterization of a spray-dried candidate HPV L2-VLP vaccine stored for multiple years at room temperature
J. Peabody (2017)
Guidance for industry: Q10 pharmaceutical cGMP regulations, US Department of Health and Human Service
Administration. UFaD (2006)
10.1016/S0169-409X(97)00067-7
Nasal delivery systems and their effect on deposition and absorption.
Vidgren (1998)
10.1089/jamp.2007.0658
Stabilizing formulations for inhalable powders of live-attenuated measles virus vaccine.
J. Burger (2008)
10.1016/j.vaccine.2017.04.026
Spray dried human and chimpanzee adenoviral-vectored vaccines are thermally stable and immunogenic in vivo.
S. Afkhami (2017)
( AERAS - 402 )
AS Tyne (2010)
prepared by spray - freeze drying induces systemic , mucosal humoral as well as cell - mediated immune responses in BALB / c mice
YF Maa (2007)
10.4155/TDE.11.82
Formulation design considerations for oral vaccines.
Jitinder S. Wilkhu (2011)
dle - free in fl uenza vaccination
JP Amorij (2010)
10.1073/pnas.1017334108
Successful respiratory immunization with dry powder live-attenuated measles virus vaccine in rhesus macaques
W. Lin (2011)
10.1021/BI00435A044
An infrared spectroscopic study of the interactions of carbohydrates with dried proteins.
J. Carpenter (1989)
10.1111/j.1749-6632.1960.tb49987.x
PRINCIPLES OF FREEZE‐DRYING
H. Meryman (1960)
10.1586/14760584.6.2.213
Pulmonary vaccine delivery
D. Lu (2007)
10.1016/j.ejpb.2008.07.015
Quality by design - Spray drying of insulin intended for inhalation.
M. Maltesen (2008)
candidate
S Cape (2014)
cine.2014.01.077. PubMed PMID: 24631078
A. Siew (2014)
pharm.2004.05.009. PubMed PMID: 15265553
MT Vidgren (2004)
10.1016/j.vaccine.2014.02.028
Implementation of QbD for the development of a vaccine candidate.
J. Haas (2014)
10.1208/s12249-016-0566-3
Physicochemical and Preclinical Evaluation of a Novel Buccal Measles Vaccine
Rikhav P. Gala (2016)
produced by spray drying
K Stahl (2010)
10.1007/s11095-007-9302-8
Poly (Lactide-co-Glycolide) Microspheres in Respirable Sizes Enhance an In Vitro T Cell Response to Recombinant Mycobacterium tuberculosis Antigen 85B
Dongmei Lu (2007)
10.1073/pnas.0800043105
Immunization by a bacterial aerosol
L. Garcia-Contreras (2008)
10.4161/hv.4.5.5863
Stabilization of measles virus for vaccine formulation
Julian M Kissmann (2008)
Induction of protective immunity against H1N1 influenza A(H1N1)pdm09 with spray-dried and electron-beam sterilised vaccines in non-human primates. Vaccine
R Scherliess (2014)
10.1208/s12248-010-9229-6
Pulmonary Immunization of Guinea Pigs with Diphtheria CRM-197 Antigen as Nanoparticle Aggregate Dry Powders Enhance Local and Systemic Immune Responses
Pavan Muttil (2010)
Improving protein stabilization by spray drying
N. Grasmeijer (2016)
Pulmonary delivery of an inulin - stabilized in fl uenza sub - unit
JP Amorij
10.1007/s11095-010-0094-x
Particle Size Influences the Immune Response Produced by Hepatitis B Vaccine Formulated in Inhalable Particles
Chandan Thomas (2010)
10.1023/A:1024493719236
Optimization of an Alum-Adsorbed Vaccine Powder Formulation for Epidermal Powder Immunization
Y. Maa (2004)
10.1021/acs.molpharmaceut.6b00072
Optimized Formulation of a Thermostable Spray-Dried Virus-Like Particle Vaccine against Human Papillomavirus.
Sugandha Saboo (2016)
10.1517/17425247.2.2.227
Epidermal delivery of protein and DNA vaccines
H. Dean (2005)
10.1016/j.ijpharm.2014.03.025
Influences of process and formulation parameters on powder flow properties and immunogenicity of spray dried polymer particles entrapping recombinant pneumococcal surface protein A.
C. Anish (2014)
10.1615/CRITREVTHERDRUGCARRIERSYST.V21.I1.20
Drug delivery to the nasal cavity: in vitro and in vivo assessment.
S. Newman (2004)
10.1586/14760584.2016.1158650
Alternative administration routes and delivery technologies for polio vaccines
H. Kraan (2016)
10.1208/s12248-010-9179-z
Preservation of the Immunogenicity of Dry-powder Influenza H5N1 Whole Inactivated Virus Vaccine at Elevated Storage Temperatures
F. Geeraedts (2010)
10.1089/10430340050015815
Adeno-associated virus vectors show variable dependence on divalent cations for thermostability: implications for purification and handling.
A. E. Turnbull (2000)
10.1016/j.jconrel.2010.02.025
A comparison between spray drying and spray freeze drying to produce an influenza subunit vaccine powder for inhalation.
V. Saluja (2010)
Safety and immunogenicity of dry powder measles
S Cape (2014)
Spray drying of protein precipitates and Evaluation of the Nano Spray Dryer B-90
Katja Schmid (2011)
10.1007/s11095-008-9575-6
Preparation of Active Proteins, Vaccines and Pharmaceuticals as Fine Powders using Supercritical or Near-Critical Fluids
S. Cape (2008)
TLR 2 - targeted secreted proteins from Mycobacterium tuberculosis are protective as powdered pulmonary
D Lu (2013)
10.1016/S0264-410X(03)00175-0
Epidermal powder immunization of mice and monkeys with an influenza vaccine.
D. Chen (2003)
10.1111/j.1365-2567.2007.02641.x
The delivery site of a monovalent influenza vaccine within the respiratory tract impacts on the immune response
Antoine Minne (2007)
10.1016/J.VACCINE.2007.10.035
Pulmonary delivery of an inulin-stabilized influenza subunit vaccine prepared by spray-freeze drying induces systemic, mucosal humoral as well as cell-mediated immune responses in BALB/c mice.
J-P. Amorij (2007)
10.1016/j.vaccine.2014.01.077
Induction of protective immunity against H1N1 influenza A(H1N1)pdm09 with spray-dried and electron-beam sterilised vaccines in non-human primates.
R. Scherließ (2014)
10.1016/j.biologicals.2014.05.007
Vaccine instability in the cold chain: mechanisms, analysis and formulation strategies.
O. S. Kumru (2014)
10.3109/1061186X.2014.888071
Cellulose acetate phthalate microparticles containing Vibrio cholerae: steps toward an oral cholera vaccine
M. Pastor (2014)
10.1006/ABBI.1997.0111
Elucidating mechanisms of thermostabilization of poliovirus by D2O and MgCl2.
C. Chen (1997)
10.1002/JPS.10135
Surface adsorption of recombinant human interferon-gamma in lyophilized and spray-lyophilized formulations.
S. D. Webb (2002)
Induction of protective immunity against H 1 N 1 in fl uenza A ( H 1 N 1 ) pdm 09 with spray - dried and electron - beam sterilised
R Scherliess
10.1016/J.VACCINE.2004.06.041
Epidermal powder immunization against influenza.
H. Dean (2004)
10.1208/s12248-010-9192-2
Immunization of Guinea Pigs with Novel Hepatitis B Antigen as Nanoparticle Aggregate Powders Administered by the Pulmonary Route
Pavan Muttil (2010)
10.1023/A:1012144810067
Effect of Glass Transition Temperature on the Stability of Lyophilized Formulations Containing a Chimeric Therapeutic Monoclonal Antibody
S. Duddu (2004)
Epidermal powder immunization of mice and monkeys with an in fl uenza
D Chen (2005)
10.1055/S-0038-1665248
Inactivation of hepatitis B and non-A, non-B viruses by combined use of Tween 80, beta-propiolactone, and ultraviolet irradiation.
A. M. Prince (1983)
10.1016/J.JCONREL.2005.09.004
The influence of formulation components on the aerosolisation properties of spray-dried powders.
Naumana R Rabbani (2005)
10.1517/17425247.1.1.67
Dry powder inhalers for pulmonary drug delivery
H. Frijlink (2004)
10.1016/j.vaccine.2011.05.098
A new oral vaccine candidate based on the microencapsulation by spray-drying of inactivated Vibrio cholerae.
G. Año (2011)
10.1002/jps.22742
Stable dry powder formulation for nasal delivery of anthrax vaccine.
S. H. Wang (2012)
Vaccine transport and storage: environmental challenges.
M. Zaffran (1996)
10.1038/nri3251
Recent progress in mucosal vaccine development: potential and limitations
N. Lycke (2012)
10.4161/hv.3.3.4011
Intranasal Administration of Dry Powder Anthrax Vaccine Provides Protection Against Lethal Aerosol Spore Challenge
J. Huang (2007)
10.1016/J.ADDR.2005.07.009
Nasal mucoadhesive drug delivery: background, applications, trends and future perspectives.
M. I. Ugwoke (2005)
Feasibility study on spraydrying protein pharmaceuticals: Recombinant human growth hormone and tissue-type plasminogen activator
M Mumenthaler (1994)
10.1016/j.ejps.2014.01.005
Improved storage stability and immunogenicity of hepatitis B vaccine after spray-freeze drying in presence of sugars.
W. Tonnis (2014)
Exploring the use of aseptic spray drying in the manufacture of biopharmaceutical injectables
A. Siew (2016)
10.4000/BOOKS.EDITIONSMSH.1745
Non-human primates
F. Joulian (2009)
Is spray drying a viable alternative to lyophilization? Equipment and Processing Report
E Greb
10.1128/JVI.77.6.3595-3601.2003
A Zinc Ion Controls Assembly and Stability of the Major Capsid Protein of Rotavirus
I. Erk (2003)
10.1023/A:1018809632395
Investigation of Protein-Surfactant Interactions by Analytical Ultracentrifugation and Electron Paramagnetic Resonance: The Use of Recombinant Human Tissue Factor as an Example
L. Jones (2004)
Pulmonary immunization using antigen 85-B polymeric microparticles to boost tuberculosis HUMAN VACCINES & IMMUNOTHERAPEUTICS 2375 immunity
D Lu
10.1016/j.vaccine.2010.04.112
Thermostable formulations of a hepatitis B vaccine and a meningitis A polysaccharide conjugate vaccine produced by a spray drying method.
D. Chen (2010)
10.3109/02652040903191834
Targeted delivery of antigens to the gut-associated lymphoid tissues: 2. Ex vivo evaluation of lectin-labelled albumin microspheres for targeted delivery of antigens to the M-cells of the Peyer's patches
J. Akande (2010)
10.1007/s11947-013-1095-7
The Contribution of the Inherent Restricted Mobility of Glassy Sugar Matrices to the Overall Stability of Freeze-Dried Bacteria Determined by Low-Resolution Solid-State 1H-NMR
M. Aschenbrenner (2013)
inventor; The United States Of America, As Represented By The Secretary, Department Of Health And Human Services, Creare Llc
Mark J. Papania JJB (2011)
10.1126/science.223.4637.701
Preservation of Membranes in Anhydrobiotic Organisms: The Role of Trehalose
J. Crowe (1984)
Induction of protective immunity against H 1 N 1 influenza A ( H 1 N 1 ) pdm 09 with spray-dried and electron-beam sterilised vaccines in non-human primates
R. Scherließ (2014)
10.1016/j.ejpb.2008.02.010
Stabilization of IgG1 in spray-dried powders for inhalation.
S. Schuele (2008)
inventorSupercritical fluid-assisted nebulization and bubble drying
Sievers RESPS (2003)
10.1023/A:1018929224005
Feasibility Study on Spray-Drying Protein Pharmaceuticals: Recombinant Human Growth Hormone and Tissue-Type Plasminogen Activator
M. Mumenthaler (2004)
Guidance for industry: Q10 pharmaceutical cGMP regulations, US Department of Health and Human Service.: FDA, Rockville
Administration. UFaD (2006)
10.1371/journal.pone.0149768
Tolerability and Pharmacokinetic Evaluation of Inhaled Dry Powder Tobramycin Free Base in Non-Cystic Fibrosis Bronchiectasis Patients
M. Hoppentocht (2016)
Monitoring vaccine wastage at country level: Guidelines for programmme managers
Who Organization (2005)
10.1586/erv.09.20
Opportunities and challenges of developing thermostable vaccines
D. Chen (2009)
10.1016/j.jconrel.2014.05.060
Buccal and sublingual vaccine delivery
H. Kraan (2014)
10.1056/NEJMOA030595
Use of the inactivated intranasal influenza vaccine and the risk of Bell's palsy in Switzerland.
M. Mutsch (2004)
10.1016/j.ijpharm.2015.09.034
Pulmonary dry powder vaccine of pneumococcal antigen loaded nanoparticles.
N. Kunda (2015)
10.1002/JPS.1032
Dry powders of stable protein formulations from aqueous solutions prepared using supercritical CO(2)-assisted aerosolization.
S. Sellers (2001)
10.1016/j.ijpharm.2015.04.053
Pharmaceutical spray freeze drying.
S. Wanning (2015)
10.1016/j.jconrel.2011.01.027
Advances in oral transmucosal drug delivery.
V. F. Patel (2011)
10.1016/J.EJPS.2005.11.013
Design and in vitro performance testing of multiple air classifier technology in a new disposable inhaler concept (Twincer) for high powder doses.
A. D. de boer (2006)
10.1007/s11095-007-9475-1
Pharmaceutical Particle Engineering via Spray Drying
R. Vehring (2007)
10.1002/JPS.10294
Stabilization of alum-adjuvanted vaccine dry powder formulations: mechanism and application.
Y. Maa (2003)
10.1016/j.ijpharm.2016.04.067
Evaluation of excipients for enhanced thermal stabilization of a human type 5 adenoviral vector through spray drying.
Daniel A. LeClair (2016)
10.1016/j.vaccine.2010.04.059
Stabilizing formulations for inhalable powders of an adenovirus 35-vectored tuberculosis (TB) vaccine (AERAS-402).
T. H. Jin (2010)
10.1021/JS960217K
Effects of bovine somatotropin (rbSt) concentration at different moisture levels on the physical stability of sucrose in freeze-dried rbSt/sucrose mixtures.
J. M. Sarciaux (1997)
10.1016/J.VIRUSRES.2004.02.027
Epidermal powder immunization: cellular and molecular mechanisms for enhancing vaccine immunogenicity.
D. Chen (2004)
10.1038/nri1728
Immunization without needles
S. Mitragotri (2005)
10.1016/0264-410X(95)00070-H
Stabilising oral poliovaccine at high ambient temperatures.
J. Newman (1996)
10.3109/02652048.2012.692402
Formulation of meningococcal capsular polysaccharide vaccine-loaded microparticles with robust innate immune recognition
Ruhi V. Ubale (2013)
A novel dry powder in fl uenza
J Huang (2007)
Implementation of quality by design in vaccine development: BioProcess international; 2013 [cited 2013 February].Web Article
P. Calcott (2013)
10.1007/s11095-016-2003-4
Optimization of Spray Drying Conditions for Yield, Particle Size and Biological Activity of Thermally Stable Viral Vectors
Daniel A. LeClair (2016)
MUCOSAL IMMUNITY AND VACCINES
R. Seipp (2013)
Induction of protective immunity against H 1 N 1 in fl uenza A ( H 1 N 1 ) pdm 09 with spraydried and electronbeam sterilised vaccines in non - human primates
R Scherliess (2014)
10.2174/1389201003378898
Biopharmaceutical powders: particle formation and formulation considerations.
Y. Maa (2000)
10.1007/s11095-016-1860-1
Stabilization of Live Attenuated Influenza Vaccines by Freeze Drying, Spray Drying, and Foam Drying
Phillip M. Lovalenti (2016)
Is spray drying a viable alternative to lyophilization ? Equipment and Processing Report ; 2009
E Greb
10.1073/pnas.0611430104
Drying a tuberculosis vaccine without freezing
Yun-Ling Wong (2007)
10.1086/426865
Protective immunization against inhalational anthrax: a comparison of minimally invasive delivery platforms.
John A. Mikszta (2005)
10.1016/j.ijpharm.2016.08.022
A Design of Experiment approach to predict product and process parameters for a spray dried influenza vaccine.
Gaurav Kanojia (2016)
Is spray drying a viable alternative to lyophilization? Equipment and Processing
E. Greb (2009)
10.1016/S0378-5173(01)00945-0
The effect of process variables on the degradation and physical properties of spray dried insulin intended for inhalation.
K. Ståhl (2002)
10.1007/s11095-008-9559-6
Development of Stable Influenza Vaccine Powder Formulations: Challenges and Possibilities
J-P. Amorij (2008)
10.1007/s11095-014-1394-3
Stabilization of HAC1 Influenza Vaccine by Spray Drying: Formulation Development and Process Scale-Up
Changcheng Zhu (2014)
10.1021/JS980175V
Tween protects recombinant human growth hormone against agitation-induced damage via hydrophobic interactions.
N. Bam (1998)
Implementation of quality by design in vaccine development
P Calcott
10.1021/mp500423z
Size and molecular flexibility of sugars determine the storage stability of freeze-dried proteins.
W. Tonnis (2015)
10.1016/J.IJPHARM.2004.05.009
Evaluation of the clearance characteristics of various microspheres in the human nose by gamma-scintigraphy.
M. Tafaghodi (2004)
10.1016/j.vaccine.2014.09.071
Safety and immunogenicity of dry powder measles vaccine administered by inhalation: a randomized controlled Phase I clinical trial.
S. Cape (2014)
Alternative vaccine delivery methods A2
BG Weniger (2013)
10.1016/j.vaccine.2009.11.024
Heat-stable measles vaccine produced by spray drying.
Satoshi Ohtake (2010)
Knaus DA. inventor; The United States Of America, As Represented By The Secretary, Department Of Health And Human Services, Creare Llc, assignee. Nasal aerosol delivery system
Mjjjb Papania (2011)
10.1016/S1473-3099(14)70845-X
Safety and immunogenicity of a candidate tuberculosis vaccine MVA85A delivered by aerosol in BCG-vaccinated healthy adults: a phase 1, double-blind, randomised controlled trial
I. Satti (2014)
10.1002/jps.23220
Immunogenicity and protection of oral influenza vaccines formulated into microparticles.
P. N. Shastri (2012)
10.3109/02652048.2011.651503
Spray-dried microparticles: a potential vehicle for oral delivery of vaccines
L. Chablani (2012)
10.1016/j.ddtec.2008.11.001
Drying methods for protein pharmaceuticals.
M. Maltesen (2008)
Effect of sorbitan monolaurate polyoxyalkylene (Tween 20) on the ultrastructure of some bacteria.
N. Cherepova (1994)
10.1208/s12248-010-9193-1
Pulmonary Immunization Using Antigen 85-B Polymeric Microparticles to Boost Tuberculosis Immunity
Dongmei Lu (2010)
inventor; The United States Of America, As Represented By The Secretary, Department Of Health And Human Services, Creare Llc,, assignee. Nasal aerosol delivery system
Papania MJJJB (2011)
inventorSupercritical fluid-assisted nebulization and bubble drying2003 October 7
R. E. Sievers SPS (2003)
administered by inhalation : A randomized controlled Phase I clinical trial
BG Weniger (2014)
10.1042/BA20030133
Freeze‐drying of proteins: some emerging concerns
I. Roy (2004)
10.1016/j.vaccine.2013.07.022
TLR2-targeted secreted proteins from Mycobacterium tuberculosis are protective as powdered pulmonary vaccines.
Anneliese S Tyne (2013)
10.1023/B:PHAM.0000048185.09483.e7
Stabilization of Proteins in Dry Powder Formulations Using Supercritical Fluid Technology
N. Jovanović (2004)
10.1002/jps.23275
Formulation and evaluation of a particulate oral breast cancer vaccine.
L. Chablani (2012)
61 -Alternative vaccine delivery methods A2 -Plotkin, Stanley A
B G Weniger (2013)
10.1007/BF01314596
The influence of divalent cations on the stability of human rotavirus
J. Shirley (2005)
10.1016/j.jconrel.2016.05.058
Evaluation of microparticulate ovarian cancer vaccine via transdermal route of delivery.
Suprita A. Tawde (2016)
Inventor Methods for fine particle formation
RE Sievers (1997)
10.1016/j.ejpb.2016.03.027
A stable live bacterial vaccine.
N. Kunda (2016)



This paper is referenced by
10.1208/s12249-020-01673-5
The Stabilizing Excipients in Dry State Therapeutic Phage Formulations
Y. Zhang (2020)
Drying Made Easy : Spray drying a promising technology for the production of stable vaccine and therapeutic protein formulations
Gaurav Kanojia (2018)
10.1080/21645515.2019.1593727
Evaluation of the thermal stability and the protective efficacy of spray-dried HPV vaccine, Gardasil® 9
N. Kunda (2019)
10.1016/j.ijpharm.2019.118615
Stabilization of HSV-2 Viral Vaccine Candidate by Spray Drying.
Daniel A. LeClair (2019)
10.3168/jds.2019-17854
Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum.
M. Lomónaco (2020)
10.1080/17425247.2020.1702643
Physical stability of dry powder inhaler formulations
Nivedita Shetty (2019)
10.1080/07373937.2019.1565576
Optimization of nano spray drying parameters for production of α-amylase nanopowder for biotheraputic applications using factorial design
H. M. Abdel-Mageed (2019)
10.1080/03639045.2019.1583758
Subunit-based mucosal vaccine delivery systems for pulmonary delivery - Are they feasible?
Nirmal Marasini (2019)
10.1080/14760584.2018.1516552
Virus-like particle vaccines: immunology and formulation for clinical translation
Braeden Donaldson (2018)
10.3389/fimmu.2019.00022
Nanoparticle-Based Vaccines Against Respiratory Viruses
Soultan Al-Halifa (2019)
Advanced polymer-based drug delivery systems for chemotherapy
B. S. Vazquez (2019)
10.1016/j.ijpharm.2019.03.047
Scale-up of pharmaceutical spray drying using scale-up rules: A review.
Sadegh Poozesh (2019)
10.1080/07373937.2020.1825293
Drying of Vaccines and Biomolecules
B. N. Thorat (2020)
10.1016/j.ejpb.2018.05.021
The effect of formulation on spray dried Sabin inactivated polio vaccine
Gaurav Kanojia (2018)
10.1007/s40005-019-00446-y
Meeting the unmet: from traditional to cutting-edge techniques for poly lactide and poly lactide-co-glycolide microparticle manufacturing
A. Schoubben (2019)
10.1016/j.nantod.2020.100961
Nanomedicine strategies to target coronavirus
M. Heinrich (2020)
10.1016/j.jconrel.2018.07.035
Development of a thermostable spray dried outer membrane vesicle pertussis vaccine for pulmonary immunization
Gaurav Kanojia (2018)
Semantic Scholar Logo Some data provided by SemanticScholar