Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

Growth Factor Control Of Skeletal Muscle Differentiation: Commitment To Terminal Differentiation Occurs In G1 Phase And Is Repressed By Fibroblast Growth Factor

C. Clegg, T. Linkhart, B. Olwin, S. Hauschka
Published 1987 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Analysis of MM14 mouse myoblasts demonstrates that terminal differentiation is repressed by pure preparations of both acidic and basic fibroblast growth factor (FGF). Basic FGF is approximately 30- fold more potent than acidic FGF and it exhibits half maximal activity in clonal assays at 0.03 ng/ml (2 pM). FGF repression occurs only during the G1 phase of the cell cycle by a mechanism that appears to be independent of ongoing cell proliferation. When exponentially growing myoblasts are deprived of FGF, cells become postmitotic within 2-3 h, express muscle-specific proteins within 6-7 h, and commence fusion within 12-14 h. Although expression of these three terminal differentiation phenotypes occurs at different times, all are initiated by a single regulatory "commitment" event in G1. The entire population commits to terminal differentiation within 12.5 h of FGF removal as all cells complete the cell cycle and move into G1. Differentiation does not require a new round of DNA synthesis. Comparison of MM14 behavior with other myoblast types suggests a general model for skeletal muscle development in which specific growth factors serve the dual role of stimulating myoblast proliferation and directly repressing terminal differentiation.
This paper references
FGF Control of Skeletal Muscle Differentiation and which is distinct from transferrin
Commitment, fusion and biochemical differentiation of a myogenic cell line in the absence of DNA synthesis
B. Nadal-Ginard (1978)
Myogenic differentiation in permanent clonal mouse myoblast cell lines: regulation by macromolecular growth factors in the culture medium.
T. Linkhart (1981)
Clonal analysis of vertebrate myogenesis. I. Early developmental events in the chick limb.
P. H. Bonner (1974)
Cell cycle dependence of differentiation in synchronous skeletal muscle myocytes
P. A. Merrifield (1984)
Clonal analysis of vertebrate myogenesis. VI. Acetylcholinesterase and acetylcholine receptor in myogenic and nonmyogenic clones from chick embryo leg cells.
T. Linkhart (1979)
Induction of myogenic differentiation in serum-free medium does not require DNA synthesis.
C. Pinset (1985)
Developmental changes preceding cell fusion during muscle differentiation in vitro.
D. Yaffe (1971)
Clonal studies of human and mouse muscle
S. D. Hauschka (1979)
Clonal analysis of vertebrate myogenesis. II. Environmental influences upon human muscle differentiation.
S. Hauschka (1974)
Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro
D. Bader (1982)
Isolation and partial molecular characterization of pituitary fibroblast growth factor.
P. Böhlen (1984)
Isolation and characterization of terminally differentiated chicken and rat skeletal muscle myoblasts.
S. F. Konieczny (1982)
Identification of the fibroblast growth factor receptor of Swiss 3T3 cells and mouse skeletal muscle myoblasts.
B. Olwin (1986)
Growth of myoblasts in hormonesupplemented serum-free medium
I. Hayashi (1982)
Growth of Cells in Hormonally Defined Media
G. Sato (1982)
Regulation of skeletal muscle satellite cell proliferation by bovine pituitary fibroblast growth factor.
R. Allen (1984)
Regulation of creatine kinase induction in differentiating mouse myoblasts.
J. Chamberlain (1985)
Reversibility of muscle differentiation in the absence of commitment: Analysis of a myogenic cell line temperature-sensitive for commitment
H. T. Nguyen (1983)
Control of myogenesis in vitro by chick embryo extract.
C. Slater (1976)
Proc. Natl. Acad. Sci. USA
Control of myogenic differentiation by fibroblast growth factor is mediated by position in the G1 phase of the cell cycle
B. Lathrop (1985)
A rapid decrease in epidermal growth factor-binding capacity accompanies the terminal differentiation of mouse myoblasts in vitro
R. Lim (1984)
Generation of chick skeletal muscle cells in groups of 16 from stem cells
L. S. Quinn (1985)
Selected muscle and nerve extracts contain an activity which stimulates myoblast proliferation Clegg et al. FGF Control of Skeletal Muscle Differentiation 955 on O cber 19
E. Kardami (1985)
Proliferation and differentiation of chick skeletal muscle cells cultured in a chemically defined medium.
P. Dollenmeier (1981)
Biochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro
D. Bader (1982)
Biphasic concentration dependency of stimulation of myoblast differentiation by somatomedins.
J. Florini (1986)
Control by fibroblast growth factor of differentiation in the BC3H1 muscle cell line
B. Lathrop (1985)
Heterokaryon analysis of muscle differentiation: regulation of the postmitotic state
C. Clegg (1987)
Selected muscle and nerve extracts contain an activity which stimulates myoblast proliferation Clegg et al. FGF Control of Skeletal Muscle Differentiation 955 and which is distinct from transferrin
E. Kardami (1985)
Population modeling in muscle cell culture: comparisons with experiments.
M. C. O'Neill (1976)
DNA synthesis in individual L-strain mouse cells.
C. Stanners (1960)
Effect of fibroblast growth factor on the division and fusion of bovine myoblasts
D. Gospodarowicz (1976)
Regulation of myogenic differentiation by type beta transforming growth factor
E. Olson (1986)
Transcriptional and posttranscriptional control of c-myc during myogenesis: its mRNA remains inducible in differentiated cells and does not suppress the differentiated phenotype.
T. Endo (1986)
Control of mouse myoblast commitment to terminal differentiation by mitogens.
T. Linkhart (1980)
Peptide antibody specific for the amino terminus of skeletal muscle alpha-actin.
J. C. Bulinski (1983)
The in vivo fusion of embryonic chick muscle without DNA synthesis
J. L. Doering (1974)
Selected muscle and nerve extracts contain an activity which stimulates myoblast proliferation Clegg et al. FGF Control of Skeletal Muscle Differentiation 955 on F ebuary 21
E. Kardami (1985)
The in vitro cell fusion of embryonic chick muscle without DNA synthesis.
J. Doering (1974)
Myogenic growth factor present in skeletal muscle is purified by heparin-affinity chromatography.
E. Kardami (1985)
Influence of environmental factors on the accumulation and differentiation of prefusion G1 lizard myoblasts in vitro.
E. Bayne (1980)
Diffusion-mediated control of myoblast fusion.
I. Konigsberg (1971)
Selected muscle and nerve extracts contain an activity which stimulates myoblast proliferation and which is distinct from transferrin.
E. Kardami (1985)
Isolation and partial characterization of pituitary fibroblast growth factor
P. Bohlen (1984)
lation and partial characterization of pituitary fibroblast growth factor
P. Bohlen (1984)
Reentry into the cell cycle of differentiated skeletal myocytes.
B. Devlin (1983)
Pathogenesis of Human Muscular Dystrophies
D. Doyle (1978)
Clonal analysis of vertebrate myogenesis
T. A. Linkhart (1979)
Selected muscle and nerve extracts contain an activity which stimulates myoblast proliferation Clegg et al. FGF Control of Skeletal Muscle Differentiation
E. Kardami (1985)
Type beta transforming growth factor is an inhibitor of myogenic differentiation.
J. Massagué (1986)
Purification of two distinct growth factors from bovine neural tissue by heparin affinity chromatography.
R. Lobb (1984)
The role of the environment in the control of myogenesis in vitro
I. R. Konigsberg (1977)
Corrections - Purification of Two Growth Factors from Bovine Neural Tissue by Heparin Affinity Chromatography
R. Lobb (1985)
Control of myosin synthesis during myoblast differentiation
C. P. Emerson (1977)
The mitogenic signaling pathway of fibroblast growth factor is not mediated through polyphosphoinositide hydrolysis and protein kinase C activation in hamster fibroblasts.
I. Magnaldo (1986)

This paper is referenced by
Fibroblast growth factor 1 induced during myogenesis by a transcription–translation coupling mechanism
C. Conte (2009)
The cellularisation of mammalian myotubes by myoseverin.
Amy Duckmanton (2004)
Quiescence and cell fate regulation are essential for preserving adult stem cell number and function
Kieran M. Jones (2014)
Evidence for cell density affecting C2C12 myogenesis: possible regulation of myogenesis by cell–cell communication
Kanako Tanaka (2011)
Skeletal muscle satellite cell proliferation in response to members of the fibroblast growth factor family and hepatocyte growth factor
S. M. Sheehan (1999)
Method of myelinating isolated motoneurons
J. Hickman (2020)
Proliferative Dynamics and the Role of FGF2 During Myogenesis of Rat Satellite Cells on Isolated Fibers.
Z. Yablonka-Reuveni (1997)
Cell density and cell cycle effects on retinoic acid-induced embryonal carcinoma cell differentiation.
R. W. Berg (1990)
A Role for Fibroblast Growth Factor in Oligodendrocyte Development
R. McKinnon (1991)
Cell type and tissue distribution of the fibroblast growth factor receptor
B. Olwin (1989)
Preliminary Studies on the Effects of Glycosyl Triazoles on C2C12 stem cells
Traci M. Clymer (2010)
Megf10 regulates the progression of the satellite cell myogenic program
C. Holterman (2007)
lnterleukin la Mediated Inhibition of Myogenic Terminal Differentiation : Increased Sensitivity of Haras Transformed Cultures ’
Maureen (2005)
Control of differentiation in BC3H1 muscle cells.
L. Glaser (1989)
Integrin oL Subunit Ratios , Cytoplasmic Domains , and Growth Factor Synergy Regulate Muscle Proliferation and Differentiation
S. K. Sastry (1996)
Evaluating Properties of Dystrophin and Delivery Methods of rAAV Gene Therapy for Duchenne Muscular Dystrophy
Julian N. Ramos (2014)
Improved medium with EGF and BSA for differentiated human skeletal muscle cells
J. A. St Clair (1992)
Expression of a single transfected cDNA converts fibroblasts to myoblasts
R. Davis (1987)
Expression of C-MYC, C-FOS and C-JUN Proto-Oncogenes During Muscle Differentiation in Vitro
N. Ringertz (1991)
Modulation of adipocyte differentiation by tumor necrosis factor and transforming growth factor beta
F. Torti (1989)
Lack of miR-378 attenuates muscular dystrophy in mdx mice
Paulina Podkalicka (2020)
Chapter 12 The molecular and cellular biology of skeletal muscle myogenesis: Practical applications
C. Pagel (1998)
Tumor necrosis factor-alpha and basic fibroblast growth factor differentially inhibit the insulin-like growth factor-I induced expression of myogenin in C2C12 myoblasts.
M. Layne (1999)
Calcineurin enhances acetylcholinesterase mRNA stability during C2-C12 muscle cell differentiation.
Z. Luo (1999)
p27Kip1 is expressed transiently in developing myotomes and enhances myogenesis.
S. Zabludoff (1998)
Growth factor control of myoepithelial-cell differentiation in cultures of human mammary gland.
O. W. Petersen (1988)
The mRNAs encoding acidic FGF, basic FGF and FGF receptor are coordinately downregulated during myogenic differentiation.
J. Moore (1991)
Basic fibroblast growth factor in the chick embryo: immunolocalization to striated muscle cells and their precursors
J. Joseph-Silverstein (1989)
Acidic and basic fibroblast growth factors in adult rat heart myocytes. Localization, regulation in culture, and effects on DNA synthesis.
E. Speir (1992)
Systems biological analyses of intracellular signal transduction
S. Legewie (2009)
The ryanodine receptor/junctional channel complex is regulated by growth factors in a myogenic cell line
A. Marks (1991)
Arginine-vasopressin induces differentiation of skeletal myogenic cells and up-regulation of myogenin and Myf-5.
C. Nervi (1995)
See more
Semantic Scholar Logo Some data provided by SemanticScholar