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ERM Family Members As Molecular Linkers Between The Cell Surface Glycoprotein CD44 And Actin-based Cytoskeletons
S. Tsukita, K. Oishi, N. Sato, J. Sagara, A. Kawai, S. Tsukita
Published 1994 · Medicine, Biology
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The ERM family members, ezrin, radixin, and moesin, localizing just beneath the plasma membranes, are thought to be involved in the actin filament/plasma membrane association. To identify the integral membrane protein directly associated with ERM family members, we performed immunoprecipitation studies using antimoesin mAb and cultured baby hamster kidney (BHK) cells metabolically labeled with [35S]methionine or surface-labeled with biotin. The results indicated that moesin is directly associated with a 140-kD integral membrane protein. Using BHK cells as antigens, we obtained a mAb that recognized the 140-kD membrane protein. We next cloned a cDNA encoding the 140-kD membrane protein and identified it as CD44, a broadly distributed cell surface glycoprotein. Immunoprecipitation with various anti-CD44 mAbs showed that ezrin and radixin, as well as moesin, are associated with CD44, not only in BHK cells, but also in mouse L fibroblasts. Furthermore, immunofluorescence microscopy revealed that in both BHK and L cells, the Triton X-100-insoluble CD44 is precisely colocalized with ERM family members. We concluded that ERM family members work as molecular linkers between the cytoplasmic domain of CD44 and actin-based cytoskeletons.
This paper references
Association of various T cell-surface molecules with the cytoskeleton. Effect of cross-linking and activation.
T. Geppert (1991)
A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells
U. Guenthert (1991)
Characterization of the class III collagen receptor, a phosphorylated, transmembrane glycoprotein expressed in nucleated human cells.
W. Carter (1988)
Splicing choice from ten variant exons establishes CD44 variability.
C. Tölg (1993)
Biochemical characterization and cellular distribution of a polymorphic, murine cell-surface glycoprotein expressed on lymphoid tissues, lmmunogenetics
I S Trowbridge (1982)
Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44
G. Koopman (1993)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
Ezrin is concentrated in the apical microvilli of a wide variety of epithelial cells whereas moesin is found primarily in endothelial cells.
M. Berryman (1993)
A new 400-kD protein
Concentration of an integral membrane protein, CD43 (leukosialin, sialophorin), in the cleavage furrow through the interaction of its cytoplasmic domain with actin-based cytoskeletons
S. Yonemura (1993)
Purification of an 80,000-dalton protein that is a component of the isolated microvillus cytoskeleton, and its localization in nonmuscle cells
A. Bretscher (1983)
A novel moesin-, ezrin-, radixin-like gene is a candidate for the neurofibromatosis 2 tumor suppressor
J. Trofatter (1993)
The functional site of erythrocyte protein 4 . 1
L. Y. W. Bourguignon (1986)
A lymphoid cell surface glycoprotein involved in endothelial cell recognition and lymphocyte homing in man
S. Jalkanen (1986)
The protein-tyrosine kinase substrate, p81, is homologous to a chicken microvillar core protein
K. L. Gould (1986)
Role of tyrosine phosphorylation in malignant transformation by viruses and in cellular growth control.
T. Hunter (1983)
CD44 is the principal cell surface receptor for hyaluronate
A. Aruffo (1990)
Alteration in a new gene encoding a putative membrane-organizing protein causes neuro-fibromatosis type 2
G. Rouleau (1993)
A heparin-binding protein involved in inhibition of smooth-muscle cell proliferation.
W. Lankes (1988)
Identification and distribution of intracellular filaments
H. Ishikawa (1979)
Perturbation of cell adhesion
Association of ERM Family Members with CD44
Identification of heat shock protein 70 in the rabies virion.
J. Sagara (1992)
Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells
T. Hunter (1981)
A new 400-kD protein from isolated adherens junctions: its localization at the undercoat of adherens junctions and at microfilament bundles such as stress fibers and circumferential bundles
S. Tsukita (1989)
Redistribution of a major cell surface glycoprotein during cell movement
K. Jacobson (1984)
Molecular linkage between cadherins and actin filaments in cell-cell adherens junctions.
S. Tsukita (1992)
The cytoplasmic tail of CD44 is required for basolateral localization in epithelial MDCK cells but does not mediate association with the detergent-insoluble cytoskeleton of fibroblasts
S. Neame (1993)
teration in a new gene encoding a putative membrane - organizing protein causes neurofibromatosis type 2
J. Sagara (1993)
Regulation of the association of membrane skeletal protein 4.1 with glycophorin by a polyphosphoinositide
R. Anderson (1985)
Radixin is a novel member of the band 4.1 family
N. Funayama (1991)
The functional site of erythrocyte protein 4.1. In Membrane Skeleton and Cy~skeletal Membrane Associations
T L Leto (1986)
Molecular cloning of protein 4.1, a major structural element of the human erythrocyte membrane skeleton.
J. Conboy (1986)
A cell surface integral membrane glycoprotein of 85,000 mol wt (gp85) associated with triton X-100-insoluble cell skeleton
G. Tarone (1984)
Glycophorin is linked by band 4.1 protein to the human erythrocyte membrane skeleton
R. Anderson (1984)
A gene family consisting of ezrin, radixin and moesin. Its specific localization at actin filament/plasma membrane association sites.
N. Sato (1992)
Actin and myosin and cell movement.
T. Pollard (1974)
Isolation of cDNA clones and complete amino acid sequence of human erythrocyte glycophorin C.
Y. Colin (1986)
Sequence and domain structure of talin
D. G. Rees (1990)
The functional site of erythrocyte protein
T. L. Leto (1986)
Extraction, purification, and analysis of messenger RNA from eukaryotic cells
J. Sambrook (1989)
Identification, cloning, and expression of a cytosolic megakaryocyte protein-tyrosinephosphatase with sequence homology to cytoskeletal protein
M. Gu (1991)
CD44--a molecule involved in leukocyte adherence and T-cell activation.
B. Haynes (1989)
A 220-kD undercoat-constitutive protein: its specific localization at cadherin-based cell-cell adhesion sites
M. Itoh (1991)
CD44 and its interaction with extracellular matrix.
J. Lesley (1993)
Molecular cloning and expression of Pgp-1. The mouse homolog of the human H-CAM (Hermes) lymphocyte homing receptor.
D. Zhou (1989)
Microvillns-specitic Mr 75,000 plasma membrane protein of human choriocarcinoma cells
R. Pakkanen (1987)
Variations in the cytoskeletal interaction and posttranslational modification of the CD44 homing receptor in macrophages
R. L. Camp (1991)
Identification, cloning, and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to cytoskeletal protein 4.1.
M. Gu (1991)
Isolation of a eDNA clone encoding a human
Q. Yang (1991)
A lymphoma plasma membrane-associated protein with ankyrin-like properties
L. Bourguignon (1986)
FMC46, a cell protrusion-associated leukocyte adhesion molecule-1 epitope on human lymphocytes and thymocytes.
L. Pilarski (1991)
Participation in normal immune responses of a metastasis-inducing splice variant of CD44.
R. Arch (1992)
Isolation of a cDNA clone encoding a human protein-tyrosine phosphatase with homology to the cytoskeletal-associated proteins band 4.1, ezrin, and talin.
Q. Yang (1991)
The lymphoma transmembrane glycoprotein GP85 (CD44) is a novel guanine nucleotide-binding protein which regulates GP85 (CD44)-ankyrin interaction.
V. Lokeshwar (1992)
cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. EMBO (Eur
K L Gould (1989)
A human lymphocyte homing receptor, the Hermes antigen, is related to cartilage proteoglycan core and link proteins
L. Goldstein (1989)
A new 82-kD barbed end-capping protein (radixin) localized in the cell- to-cell adherens junction: purification and characterization
S. Tsukita (1989)
Moesin, like ezrin, colocalizes with actin in the cortical cytoskeleton in cultured cells, but its expression is more variable.
Z. Franck (1993)
Spontaneous redistribution of cell-surface glycoproteins in lymphoid cells during cytokinesis.
S. de Petris (1984)
Bourguignon. 1992. A CD44-1ike endothelial cell transmembrane glycoprotein (GPll6) interacts with extracellular matrix and ankyrin
L Y W Bourguignun
cDNA cloning and sequencing of the protein‐tyrosine kinase substrate, ezrin, reveals homology to band 4.1.
K. L. Gould (1989)
Rapid phosphorylation and reorganization of ezrin and spectrin accompany morphological changes induced in A-431 cells by epidermal growth factor
A. Bretscher (1989)
Moesin: a member of the protein 4.1-talin-ezrin family of proteins.
W. Lankes (1991)
Mouse T lymphoma cells contain a transmembrane glycoprotein (GP85) that binds ankyrin
E. Kalomiris (1988)
The hyaluronate receptor is associated with actin filaments
B. Lacy (1987)
The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa
E. Wayner (1988)
Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons.
G. Screaton (1992)
Molecular cloning and expression of Pgp-1
Phosphorylation of CD44 in vivo requires both Ser323 and Ser325, but does not regulate membrane localization or cytoskeletal interaction in epithelial cells.
S. Neame (1992)
cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4
K L Gould (1989)
Radixin, a barbed end-capping actin-modulating protein, is concentrated at the cleavage furrow during cytokinesis [published erratum appears in J Cell Biol 1991 Sep;114(5):1101-3]
N. Sato (1991)
The transmembrane hyaluronate receptor (CD44): multiple functions, multiple forms.
B. Haynes (1991)
Cytovillin, a microvillar Mr 75,000 protein. cDNA sequence, prokaryotic expression, and chromosomal localization.
O. Turunen (1989)
A CD44-like endothelial cell transmembrane glycoprotein (GP116) interacts with extracellular matrix and ankyrin.
L. Bourguignon (1992)
The Cell Surface
Albert Eugene Kahn (1974)
Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members
K. Takeuchi (1994)
The spectrin-actin junction of erythrocyte membrane skeletons.
V. Bennett (1989)
This paper is referenced by
Chapter 14 Interactions between the Membrane-Cytoskeleton and CD44 during Lymphocyte Signal Transduction and Cell Adhesion
L. Bourguignon (1996)
Age-Related Defects in Moesin/Ezrin Cytoskeletal Signals in Mouse CD4 T Cells1
G. G. García (2007)
CD44 Interaction with c-Src Kinase Promotes Cortactin-mediated Cytoskeleton Function and Hyaluronic Acid-dependent Ovarian Tumor Cell Migration*
L. Bourguignon (2001)
Expression of cell adhesion molecules in oesophageal carcinoma and its prognostic value
K. Nair (2005)
CD44 directs membrane‐type 1 matrix metalloproteinase to lamellipodia by associating with its hemopexin‐like domain
H. Mori (2002)
Activated radixin is essential for GABAA receptor α5 subunit anchoring at the actin cytoskeleton
Sven Loebrich (2006)
GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila.
K. Edwards (1997)
Involvement of CD44 in cytoskeleton rearrangement and raft reorganization in T cells.
N. Föger (2001)
A systematic screen for dominant second-site modifiers of Merlin/NF2 phenotypes reveals an interaction with blistered/DSRF and scribbler.
D. LaJeunesse (2001)
Prediction of the structure of human Janus kinase 2 (JAK2) comprising JAK homology domains 1 through 7.
Fabrizio Giordanetto (2002)
Expression of ezrin in prostatic intraepithelial neoplasia.
See-Tong Pang (2004)
Therapeutic Targeting of Hyaluronan in the Tumor Stroma
Anne Kultti (2012)
Fer tyrosine kinase oligomer mediates and amplifies Src-induced tumor progression
C. Oneyama (2016)
E-cadherin Negatively Regulates CD44-Hyaluronan Interaction and CD44-mediated Tumor Invasion and Branching Morphogenesis*
Y. Xu (2003)
A mechanism underlying the movement requirement for synovial joint cavitation.
G. Dowthwaite (2003)
The Number of Ezrin-Expressing Lymphocytes Correlating with Tumor Cell Apoptosis and Prognosis of Human Gastric Carcinoma
T. Matsubara (2014)
Assembly and composition of the cellular actin cortex
M. Bovellan (2012)
Chapter 13 – Signal Transduction to and from Adhesion Molecules
B. Gomperts (2009)
Neuroblastoma: Current Imaging and Therapeutics
A. Patel (2015)
CD44 is prognostic for overall survival in the NCI randomized trial on breast conservation with 25 year follow-up
T. Dan (2013)
Evidence of involvement of CD44 in endothelial cell proliferation, migration and angiogenesis in vitro
V. Trochon (1996)
Microtubule Function in Morphological Differentiation: Growth Zones and Growth Cones
Leticia R. Vega (1997)
A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells
B. Straub (2003)
Ezrin directly interacts with AQP2 and promotes its endocytosis
W. Li (2017)
Peripheral astrocyte processes: Monitoring by selective immunostaining for the actin‐binding ERM proteins
A. Derouiche (2001)
Ultrastructural Analysis of Human Epidermal CD44 Reveals Preferential Distribution on Plasma Membrane Domains Facing the Hyaluronan-rich Matrix Pouches
A. Tuhkanen (1998)
CD44 and adhesion of normal and leukemic CD34+ cells to bone marrow stroma.
L. Bendall (1999)
Protein Phosphatase 2C Inactivates F-actin Binding of Human Platelet Moesin*
A. Hishiya (1999)
Hierarchy of Merlin and Ezrin N- and C-terminal Domain Interactions in Homo- and Heterotypic Associations and Their Relationship to Binding of Scaffolding Proteins EBP50 and E3KARP*
R. Nguyen (2001)
Ezrin gene, coding for a membrane-cytoskeleton linker protein, is regionally expressed in the developing mouse neuroepithelium.
L. Gimeno (2004)
CD44v6 promotes proliferation by persisting activation of MAP kinases.
R. Marhaba (2005)
The actin-cytoskeleton linker protein ezrin is regulated during osteosarcoma metastasis by PKC
L. Ren (2009)See more