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NONDROPLET ULTRASTRUCTURAL DEMONSTRATION OF CYTOCHROME OXIDASE ACTIVITY WITH A POLYMERIZING OSMIOPHILIC REAGENT, DIAMINOBENZIDINE (DAB)

A. Seligman, M. Karnovsky, H. Wasserkrug, J. Hanker
Published 1968 · Biology, Medicine

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A new method for demonstrating cytochrome oxidase activity, based upon the oxidative polymerization of 3,3'-diaminobenzidine (DAB) to an osmiophilic reaction product, has improved the localization of this enzyme over methods based upon the Nadi reaction, in both the light and electron microscopes. The reaction product occurs in nondroplet form, which more accurately delineates the localization of cytochrome oxidase in mitochondria of heart, liver, and kidney. In electron microscopic preparations the excess reaction product is found to overflow into the intracristate spaces and into the outer compartment between inner and outer limiting mitochondrial membranes. This finding suggests that the enzymatic activity of cytochrome c is located on the inner surface of the intracristate space which is the outer surface of the inner mitochondrial membrane. Succinic dehydrogenase activity has also been located at this site by using an osmiophilic ditetrazolium salt, TC-NBT. Considered together, the sites of reactivity of both parts of the respiratory chain have implications for the chemiosomotic hypothesis of Mitchell who suggests a mechanism of energy conservation during electron transport in the respiratory chain of the mitochondrion.
This paper references
10.1177/001452469000101110
"J."
G. G. Stokes (1890)
Ber. Deut. Chem. Ges
Willstx4tter (1906)
Ber. Deut. Chem. Ges
Willstx4tter (1906)
Ber. Deut. Chem. Ges. THE JOURNAL OF CELL BIOLOGY
Willstx4tter (1906)
Ber. Deut. Chem. Ges
Willstxtter (1909)
Biochem
R E Davies (1950)
10.1177/6.6.445
THE HISTOCHEMICAL DEMONSTRATION OF CYTOCHROME OXIDASE WITH A NEW REAGENT FOR THE NADI REACTION
M. Nachlas (1958)
J. Histochem. Cytochem
M M Nachlas (1958)
10.1177/7.2.112
NEW HISTOCHEMICAL TECHNIQUES FOR THE DEMONSTRATION OF TISSUE OXIDASE (CYTOCHROME OXIDASE)
M. S. Burstone (1959)
J. Histochem. Cytochem
M S Burstone (1959)
10.3109/10520296009114754
Embedding in epoxy resins for ultrathin sectioning in electron microscopy.
K. Richardson (1960)
10.1016/s0021-9258(19)76946-4
Sites of electron transfer to tetrazolium salts in the succinoxidase system.
M. Nachlas (1960)
J. Biol. Chem
M M Nachlas (1960)
J. Biol. Chem
M M Nachlas (1960)
Stain Technol
K C Richardson (1960)
10.1038/191144A0
Coupling of Phosphorylation to Electron and Hydrogen Transfer by a Chemi-Osmotic type of Mechanism
P. Mitchell (1961)
10.1126/science.146.3647.1039
Osmiophilic Reagents: New Cytochemical Principle for Light and Electron Microscopy
J. Hanker (1964)
10.1177/12.6.462
FACTORS AFFECTING THE CYTOCHEMICAL REACTION OF PEROXIDASE WITH BENZIDINE AND THE STABILITY OF THE BLUE REACTION PRODUCT
W. Straus (1964)
The Mitochondrion
Lehninger (1964)
J. Histochem. Cytochem
Straus (1964)
Histological Techniques for SELIGMAN, KARNOVSKY, WASSERKRUG, AND HANKER Cytochrome Oxidase Activity 13 Electron Microscopy
D. C. PEASE (1964)
10.1083/JCB.30.2.424
A NEW STAINING METHOD (OTO) FOR ENHANCING CONTRAST OF LIPID-CONTAINING MEMBRANES AND DROPLETS IN OSMIUM TETROXIDE-FIXED TISSUE WITH OSMIOPHILIC THIOCARBOHYDRAZIDE (TCH)
A. Seligman (1966)
10.1177/14.4.291
THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE
R. Graham (1966)
10.1083/JCB.30.2.269
ULTRASTRUCTURAL BASES FOR METABOLICALLY LINKED MECHANICAL ACTIVITY IN MITOCHONDRIA
C. R. Hackenbrock (1966)
J. Cell Biol
A M Seligman (1966)
J. Cell Biol
Hackenbrock (1966)
J. Cell Biol
Hackenbrock (1966)
10.1083/JCB.32.2.415
AN ELECTRON-TRANSPORT SYSTEM ASSOCIATED WITH THE OUTER MEMBRANE OF LIVER MITOCHONDRIA
G. Sottocasa (1967)
10.1083/JCB.34.3.787
ULTRASTRUCTURAL DEMONSTRATION OF CYTOCHROME OXIDASE ACTIVITY BY THE NADI REACTION WITH OSMIOPHILIC REAGENTS
A. Seligman (1967)
10.1056/nejm196705182762006
The mitochondrion.
D. Tapley (1967)
10.1177/15.12.752
OBSERVATIONS ON MITOCHONDRIA STAINING FOR SUCCINIC DEHYDHOGENASE
G. Haydon (1967)
10.1177/15.1.1
ELECTRON MICROSCOPIC DEMONSTRATION OF DEHYDROGENASE ACTIVITY WITH A NEW OSMIOPHILIC DITETRAZOLIUM SALT (TC-NBT)
A. Seligman (1967)
10.1083/JCB.32.3.719
The submitochondrial localization of monoamine oxidase. An enzymatic marker for the outer membrane of rat liver mitochondria.
C. Schnaitman (1967)
Proton-translocation phosphorylation in mitochondria, chloroplasts and bacteria: natural fuel cells and solar cells.
P. Mitchell (1967)
Energy coupling in electron transport.
Lehninger Al (1967)
Control and conservation of energy in the cytochrome chain.
B. Chance (1967)
Histochemie
S Kerpel-Fronius (1967)
J. Cell Biol
Sottocasa (1967)
J. Histochem. Cytochem. Histochemie
Haydon (1967)
Histochemie
S Kerpel-Fronius (1967)
J. Cell Biol
C Schnaitman (1967)
Federation Proc
Chance (1967)
J. Cell Biol
Schnaitman (1967)
J. Cell Biol
Sottocasa (1967)
J. Histochem. Cytochem
A M Seligman (1967)
J. Histochem. Cytochem
Haydon (1967)
J. Cell Biol. J. Histochem. Cytochem
A M Seligman (1967)
Federation Proc
Mitchell (1967)
Federation Proc
Lehninger (1967)
Histochemie
Plapinger (1968)
Histochemie
Plapinger (1968)
Cytochrome Oxidase Activity 13 on April 13, 2017 Downloaded from Published July 1, 1968 Electron Microscopy
Karnovsky Seligman
J. Cell Biol
J. Cell Biol
J. Histochem. Cytochem
Cytochrome Oxidase Activity Electron Microscopy
Karnovsky Seligman



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10.1007/978-1-0716-1433-4_6
Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections.
M. Wieckowski (2021)
10.1039/d0mh01579b
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Maren T. Kuchenbrod (2021)
10.1021/jacs.1c04236
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Seunghyeon Kim (2021)
10.1111/nph.16606
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Robin Nostadt (2020)
10.1002/ar.24409
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S. Haidarliu (2020)
10.1038/s41598-020-61381-9
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S. Dijk (2020)
10.1111/tpj.14762
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D. E. Gras (2020)
10.1007/s10811-019-01962-y
The role of catalase in assimilation of exogenous methanol by Chlamydomonas reinhardtii cells
S. Stepanov (2020)
10.1016/bs.mcb.2019.11.024
Histochemical and immunohistochemical staining methods to visualize mitochondrial proteins and activity.
Sandra Franco-Iborra (2020)
10.1111/jmi.12891
Mitochondrial morphology and function: two for the price of one!
J. Faitg (2020)
10.1016/j.cels.2019.12.002
Molecular and Functional Networks Linked to Sarcopenia Prevention by Caloric Restriction in Rhesus Monkeys.
T. Rhoads (2020)
10.1016/j.jtherbio.2019.102469
Thermal tolerance of dried shoots of the moss Bryum argenteum.
L. Zhuo (2020)
10.1007/s00436-020-06798-x
Electron microscopy cytochemistry and three-dimensional reconstruction of labeled structures in Trypanosoma cruzi
W. de Souza (2020)
10.1093/cercor/bhy072
Densities and Laminar Distributions of Kv3.1b-, PV-, GABA-, and SMI-32-Immunoreactive Neurons in Macaque Area V1.
J. Kelly (2019)
10.1007/s11103-019-00825-x
CYSTM3 negatively regulates salt stress tolerance in Arabidopsis
Yang Xu (2019)
10.1016/b978-0-7020-6864-5.00018-9
Neuropathology and muscle biopsy techniques
J. Highley (2019)
10.1038/s41598-019-42902-7
Cerebral mitochondrial electron transport chain dysfunction in multiple system atrophy and Parkinson’s disease
Sandrine C. Foti (2019)
10.1016/j.cub.2019.05.040
The Foveal Visual Representation of the Primate Superior Colliculus
Chih-Yang Chen (2019)
10.1007/978-1-4939-9072-6_19
A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodendrocytes.
G. Campbell (2019)
10.1016/j.jplph.2019.153089
Functional identification of apple MdMYB2 gene in phosphate-starvation response.
Yu-Ying Yang (2019)
10.1083/jcb.201901097
Coupling APEX labeling to imaging mass spectrometry of single organelles reveals heterogeneity in lysosomal protein turnover
D. Narendra (2019)
10.1101/554121
The foveal visual representation of the primate superior colliculus
Chih-Yang Chen (2019)
10.1091/mbc.E19-08-0450
Coordinated organization of mitochondrial lamellar cristae and gain of COX function during mitochondrial maturation in Drosophila
Yifan Jiang (2019)
10.1016/j.ajpath.2018.09.007
AP39, a Modulator of Mitochondrial Bioenergetics, Reduces Antiangiogenic Response and Oxidative Stress in Hypoxia-Exposed Trophoblasts: Relevance for Preeclampsia Pathogenesis.
Ambart E. Covarrubias (2019)
10.3389/fmicb.2018.00068
Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S
Satoshi Kawaichi (2018)
10.1038/s41598-018-19745-9
3,3′-Diaminobenzidine staining interferes with PCR-based DNA analysis
Christian Dölle (2018)
10.1101/503474
Mitochondrial cristae biogenesis coordinates with ETC complex IV assembly during Drosophila maturation
Yi-fan Jiang (2018)
10.3390/ijms19092813
Deletion of Bmal1 Prevents Diet-Induced Ectopic Fat Accumulation by Controlling Oxidative Capacity in the Skeletal Muscle
T. Wada (2018)
10.3390/jcm6070064
Myopathology of Adult and Paediatric Mitochondrial Diseases
R. Phadke (2017)
10.20944/PREPRINTS201706.0059.V1
Pathology of Adult and Paediatric Mitochondrial Myopathies
R. Phadke (2017)
10.1186/s13395-017-0139-5
Utrophin influences mitochondrial pathology and oxidative stress in dystrophic muscle
Tahnee L. Kennedy (2017)
10.3389/fcvm.2017.00043
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S. Delbosc (2017)
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