Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Incorporation Of Axonally Transported Glycoproteins Into Axolemma During Nerve Regeneration

J. Griffin, D. Price, D. Drachman, J. Morris
Published 1981 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The insertion of axonally transported fucosyl glycoproteins into the axolemma of regenerating nerve sprouts was examined in rat sciatic motor axons at intervals after nerve crush. [(3)H]Fucose was injected into the lumbar ventral horns and the nerves were removed at intervals between 1 and 14 d after labeling. To follow the fate of the “pulse- labeled” glycoproteins, we examined the nerves by correlative radiometric and EM radioautographic approaches. The results showed, first, that rapidly transported [(3)H]fucosyl glycoproteins were inserted into the axolemma of regenerating sprouts as well as parent axons. At 1 d after delivery, in addition to the substantial mobile fraction of radioactivity still undergoing bidirectional transport within the axon, a fraction of label was already associated with the axolemma. Insertion of labeled glycoproteins into the sprout axolemma appeared to occur all along the length of the regenerating sprouts, not just in sprout terminals. Once inserted, labeled glycoproteins did not undergo extensive redistribution, nor did they appear in sprout regions that formed (as a result of continued outgrowth) after their insertion. The amount of radioactivity in the regenerating nerves decreased with time, in part as a result of removal of transported label by retrograde transport. By 7-14 d after labeling, radioautography showed that almost all the remaining radioactivity was associated with axolemma. The regenerating sprouts retained increased amounts of labeled glycoproteins; 7 or 14 d after labeling, the regenerating sprouts had over twice as much of radioactivity as comparable lengths of control nerves or parent axons. One role of fast axonal transport in nerve regeneration is the contribution to the regenerating sprout of glycoproteins inserted into the axolemma; these membrane elements are added both during longitudinal outgrowth and during lateral growth and maturation of the sprout.
This paper references
Stimulation of thymidine incorporation in glia by neuronal sonicates
J. E. Heuser (1973)
Smooth endoplasmic reticulum and fast axonal transport of glycoproteins . An electron microscope radioautographic study of thick sections after heavy metals impregnation
D Markov (1976)
10.1016/0014-4886(80)90006-0
Effects of aging on nerve sprouting and regeneration
A. Pestronk (1980)
Distribution of leucine-'H during axoplasmic transport within regenerating neurons as determined by electron microscopic autoradiography
T. Lentz (1972)
Stimulation of Schwann cell proliferation by neurite membrane fraction
J. L. Salzer (1977)
10.1083/JCB.16.1.73
ELECTRON MICROSCOPIC AUTORADIOGRAPHY An Improved Technique for Producing Thin Films and Its Application to H3-Thymidine-Labeled Maize Nuclei
J. Koehler (1963)
10.1007/978-1-4613-2925-1_12
Axonal Transport of Complex Carbohydrates
J. Elam (1979)
10.1016/0006-8993(72)90187-4
Rapid axonal transport of ( 3 H)fucosyl glycoproteins in the goldfish optic system.
D. Forman (1972)
10.1038/244093a0
Model for Membrane Movements in the Neural Growth Cone
D. Bray (1973)
10.1016/0006-8993(74)90941-X
The axonal transport of slowly migrating (3H)leucine labelled proteins and the regeneration rate in regenerating hypoglossal and vagus nerves of the rabbit.
M. Frizell (1974)
10.1016/0006-8993(71)90138-7
Reversed polarity of rapid axonal transport in chicken motoneurons.
J. Bray (1971)
10.1016/0006-8993(74)90405-3
Injection of radioactive materials into an identified axon of Aplysia.
S. Treistman (1974)
Smooth endoplasmic reticulum and fast axonal transport of glycoproteins . An electron microscope radioautographic study of thick sections after heavy metals impregnation
I. McQuarrie (1976)
Axonal transport of complex carbohydrates In Complex Carbohydrates of Nervous Tissue
Elam
10.1007/BF01178226
Initial endocytosis of peroxidase or ferritin by growth cones of cultured nerve cells
M. Bunge (1977)
10.1016/0013-4694(60)90053-5
Degeneration and regeneration of the nervous system
M. Scheibel (1960)
10.1016/0006-8993(74)90357-6
The axonal transport of (3H)fucose labelled glycoproteins in normal and regenerating peripheral nerves.
M. Frizell (1974)
10.1016/0006-8993(76)90283-3
A radioautographic analysis in the light and electron microscope of identified Aplysia neurons and their processes after intrasomatic injection of l-[3H]fucose
E. B. Thompson (1976)
10.1016/0006-8993(78)91116-2
The effect of a conditioning lesion on the regeneration of motor axons
I. Mcquarrie (1978)
Insertio n of radioactive materials into an identified axon of Aplysia. Brain Res
Triestman (1974)
10.1097/00005072-197705000-00148
THE DISTRIBUTION OF AXONALLY TRANSPORTED GLYCOPROTEINS IN REGENERATING AXONS: 132
A. Tessler (1977)
Evidenc e for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction
J E Heuser (1973)
Resolution in electron microscopic autoradiography
L. Glaser (1969)
10.1111/j.1471-4159.1974.tb04388.x
RETROGRADE AXONAL TRANSPORT OF RAPIDLY MIGRATING PROTEINS IN THE VAGUS AND HYPOGLOSSAL NERVES OF THE RABBIT
M. Frizell (1974)
10.1016/0014-4886(69)90093-4
Transport of protein in goldfish optic nerve during regeneration.
B. Grafstein (1969)
10.1016/0006-8993(78)90504-8
Regeneration of motor axons in the rat sciatic nerve studied by labeling with axonally transported radioactive proteins
D. Forman (1978)
The axonal transport of slowly migrating ('H)-leucine
Frizell (1974)
10.1111/j.1471-4159.1977.tb09624.x
REVERSAL OF AXONAL TRANSPORT AT A NERVE CRUSH 1
M. Bisby (1977)
10.1002/NEU.480070208
Membrane marker movement on sympathetic axons in tissue culture.
L. Koda (1976)
Resolution in electron microscopic autoradiography
M M Salpeter (1969)
10.1016/0006-8993(80)90144-4
Axonal transport of the cytoskeleton in regenerating motor neurons: constancy and change
P. Hoffman (1980)
10.1002/NEU.480070407
Fast axonal transport in motor nerve regeneration.
J. Griffin (1976)
10.1016/0006-8993(73)90852-4
Axonal migration of protein and glycoprotein to nerve endings. I. Radioautographic analysis of the renewal of protein in nerve endings of chicken ciliary ganglion after intracerebral injection of (3H)lysine.
B. Droz (1973)
10.1038/125230a0
Degeneration and Regeneration of the Nervous System
D. Db (1930)
Stimulation of thymidine incorporation in glia by neuronal sonicates
G Hanson (1977)
10.1016/0006-8993(75)90082-7
Axonal transport in embryonic neurons. The possibility of a proximo-distal axolemmal transfer of glycoproteins
P. Marchisio (1975)
Fast axonal transport in the fibers of chromatolysed neurons
S Ochs (1976)
10.1016/0006-8993(73)90854-8
Axonal migration of protein and glycoprotein to nerve endings. 3. Cell fraction analysis of chicken ciliary ganglion after intracerebral injection of labeled precursors of proteins and glycoproteins.
L. Di Giamberardino (1973)
10.1002/NEU.480080407
Impaired axonal regeneration in acrylamide intoxication.
J. Griffin (1977)
10.1016/0014-4886(78)90247-9
Fast axonal transport of labeled protein in sensory axons during regeneration
M. Bisby (1978)
10.1016/b978-1-4831-6739-8.50012-4
ELECTRON MICROSCOPIC AUTORADIOGRAPHY
H. Sheldon (1964)
10.1016/0006-8993(75)90282-6
The smooth endoplasmic reticulum: structure and role in the renewal of axonal membrane and synaptic vesicles by fast axonal tranport
B. Droz (1975)
10.1111/j.1471-4159.1971.tb05079.x
RAPID INTRACELLULAR TRANSPORT OF FUCOSE‐CONTAINING GLYCOPROTEINS IN RETINAL GANGLION CELLS
J. O. Karlsson (1971)
On the origin and fate of external acetylchohnesterase in peripheral nerve
S Brimijoin (1978)
Smooth endoplasmic reticulum and fast axonal transport of glycoproteins
D. Markov (1976)
10.1097/00005072-197703000-00002
THE PATHOGENESIS OF REACTIVE AXONAL SWELLINGS: ROLE OF AXONAL TRANSPORT
J. Griffin (1977)
10.1016/0006-8993(73)90853-6
Axonal migration of protein and glycoprotein to nerve endings. II. Radioautographic analysis of the renewal of glycoproteins in nerve endings of chicken ciliary ganglion after intracerebral injection of (3H)fucose and (3H)-glucosamine.
G. Bennett (1973)
Axonal transport of complex carbohydrates . In Complex Carbohydrates of Nervous Tissue . R . K . Margolis and R . U . Margolis
M. Estridge (1978)
10.1083/JCB.66.2.351
The slow component of axonal transport. Identification of major structural polypeptides of the axon and their generality among mammalian neurons
P. Hoffman (1975)
10.1083/JCB.79.1.138
Compositional analysis of growing axons from rat sympathetic neurons
M. Estridge (1978)
10.1083/JCB.63.1.180
FREEZE-FRACTURING OF NERVE GROWTH CONES AND YOUNG FIBERS
K. Pfenninger (1974)
Impaired axonal regeneration
Griffin (1977)



This paper is referenced by
10.1523/JNEUROSCI.06-05-01365.1986
Analysis of the carbohydrate composition of axonally transported glycoconjugates in sciatic nerve
C. Hart (1986)
10.1002/ANA.410230103
Axonal transport in neurological disease
J. Griffin (1988)
10.1002/NEU.480230916
Neuronal degeneration in human diseases and animal models.
D. Price (1992)
10.1523/JNEUROSCI.02-10-01412.1982
Axonal transport and transcellular transfer of nucleosides and polyamines in intact and regenerating optic nerves of goldfish: speculation on the axonal regulation of periaxonal cell metabolism
N. Ingoglia (1982)
10.1002/MUS.880110906
Conduction studies in peripheral cat nerve using implanted electrodes: II. The effects of prolonged constriction on regeneration of crushed nerve fibers
C. Krarup (1988)
10.1016/0006-8993(87)91325-4
A glial-neuronal-glial communication system in the mammalian central nervous system
K. Berkley (1987)
10.1083/JCB.108.2.613
Posttranslational membrane attachment and dynamic fatty acylation of a neuronal growth cone protein, GAP-43
J. Skene (1989)
10.1111/j.1471-4159.1983.tb08103.x
Glycosylation as a Criterion for Defining Subpopulations of Fast‐Transported Proteins
G. Stone (1983)
10.1016/0896-6273(89)90242-0
Growth of sympathetic nerve fibers in culture does not require extracellular calcium
R. Campenot (1989)
10.1111/j.1471-4159.1983.tb04796.x
Characterization of Axonally Transported Glycoproteins in Regenerating Garfish Olfactory Nerve
G. Cole (1983)
10.1016/S0896-6273(00)80083-5
Acceleration of Membrane Recycling by Axotomy of Cultured Aplysia Neurons
U. Ashery (1996)
10.1523/JNEUROSCI.09-02-00436.1989
Transport of cytoskeletal elements from parent axons into regenerating daughter axons
I. Mcquarrie (1989)
10.1016/0092-8674(95)90182-5
Axon membrane flows from the growth cone to the cell body
J. Dal (1995)
10.1111/j.1471-4159.1987.tb04115.x
Subsets of Axonally Transported and Periaxonal Polypeptides Are Released from Regenerating Nerve
B. Tedeschi (1987)
10.1007/978-3-7091-6595-9_20
Infantile neuroaxonal dystrophy associated with α-N-acetylgalactosaminidase deficiency: on relating axonal spheroids to a lysosomal enzyme deficiency
D. Wolfe (1995)
10.1007/978-3-642-68632-0
Repair and Regeneration of the Nervous System
J. Nicholls (1982)
10.1007/BF03159725
Intracellular transport and neuronal activation of phospholipid and glycoprotein synthesis during axonal regeneration of cranio-spinal nerves.
M. Alberghina (1989)
10.1038/336672A0
Development of neuronal polarity: GAP-43 distinguishes axonal from dendritic growth cones
K. Goslin (1988)
10.1016/0892-0354(88)90002-0
The ultrastructure of the neuronal growth cone: new insights from subcellular fractionation and rapid freezing studies.
P. R. Gordon-Weeks (1988)
10.1523/JNEUROSCI.05-07-01755.1985
Slow axonal transport in acrylamide neuropathy: different abnormalities produced by single-dose and continuous administration
B. Gold (1985)
10.1006/nbdi.1994.0002
Motor neurone disease and animal models
D. Price (1994)
10.1038/375592A0
Preferential addition of newly synthesized membrane protein at axonal growth cones
A. Craig (1995)
10.1007/BF01188405
Schwann cell proliferation following lysolecithin-induced demyelination
J. Griffin (1990)
10.3109/02713688908997567
Quantitative analysis of labelled inner retinal proteins in experimental optic neuritis.
J. Guy (1989)
10.1016/0006-8993(87)90643-3
Fast axonally transported proteins in regenerating goldfish optic nerve: effect of abolishing electrophysiological activity with TTX
E. Antonian (1987)
10.1002/CNE.902240103
Postnatal differentiation of rat optic nerve fibers: Electron microscopic observations on the development of nodes of Ranvier and axoglial relations
C. Hildebrand (1984)
10.1111/j.1471-4159.1985.tb10554.x
Retrograde Axonal Transport of Phospholipid in Rat Sciatic Nerve
M. Bisby (1985)
10.1007/978-1-4684-1197-3_1
Axoplasmic Transport in Relation to Nerve Fiber Regeneration
S. Ochs (1984)
10.1007/978-3-642-68632-0_19
Early Responses to Neural Injury
H. J. Anderson (1982)
10.1016/j.expneurol.2009.12.033
Measuring nerve regeneration in the mouse
J. Griffin (2010)
10.1016/0306-4522(95)00463-7
Ultrastructural localization of B-50/growth-associated protein-43 to anterogradely transported synaptophysin-positive and calcitonin gene-related peptide-negative vesicles in the regenerating rat sciatic nerve
P. Verkade (1996)
10.1523/JNEUROSCI.08-07-02596.1988
Local role of Ca2+ in formation of veils in growth cones
D. Goldberg (1988)
See more
Semantic Scholar Logo Some data provided by SemanticScholar