Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

In Vivo Evaluation Of Whey Protein-based Biofilms As Scaffolds For Cutaneous Cell Cultures And Biomedical Applications.

M. Rouabhia, V. Gilbert, Hongxum Wang, M. Subirade
Published 2007 · Materials Science, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
This study evaluated the toxicity, biodegradability and immunogenicity of newly developed whey protein-based biofilms for possible use as biomaterials for medical applications. Biofilms were prepared using (A) a whey protein isolate plasticized with either diethylene glycol (DEG) or glycerol (GLY), and (B) beta-lactoglobulin (betaLGA) plasticized with DEG. The biofilms were implanted subcutaneously into Balb/c mice. Analyses were performed at various time points. At 15, 30 and 60 days post-implantation, no necrotic zones or exudates were present at the recipient sites. The biofilms began to degrade as early as 15 days post-implantation, as evidenced by erosion and crumbling. The macroscopic observations were supported by tissue analyses revealing no tissue necrosis or degradation and confirming that the biodegradation of the biofilms began as early as 15 days post-implantation and was almost complete after 60 days. The biodegradation was accompanied by significant leukocyte infiltration at 15 days which significantly decreased at 60 days. The absence of splenomagaly in the implanted mice confirms that these biofilms were not immunogenic. Whey protein-based biofilms are biocompatible and biodegradable and may be of interest for medical applications such as scaffolds for cutaneous cell cultures and skin recovery in burn patients.
This paper references
10.1016/J.BIOMATERIALS.2005.05.085
Characterization and evaluation of whey protein-based biofilms as substrates for in vitro cell cultures.
V. Gilbert (2005)
10.1016/J.BURNS.2004.11.008
The evolving characteristics and care of necrotizing soft-tissue infections.
Frederick W. Endorf (2005)
10.1016/S0142-9612(00)00078-8
A comparison of the inflammatory response to a polydimethylsiloxane implant in male and female Balb/c mice.
A. Dalu (2000)
10.1016/S1386-6346(03)00301-2
Erratum to “Progression of autoimmune-mediated hepatic lesions in a murine graft-versus-host reaction by neutralizing IL-10” [Hepatology Research 25 (2003) 423–434]
R. Unno (2003)
In vitro production and transplantation of immunologically active skin equivalents.
M. Rouabhia (1996)
Use of a composite skin graft composed of cultured human keratinocytes and fibroblasts and a collagen-GAG matrix to cover full-thickness wounds on athymic mice.
Cooper Ml (1991)
10.1016/S1386-6346(02)00308-X
Progression of autoimmune-mediated hepatic lesions in a murine graft-versus-host reaction by neutralizing IL-10.
R. Unno (2003)
Update on dermal substitutes.
M. Ehrenreich (2006)
10.1016/S0142-9612(03)00161-3
Biocompatibility of implantable synthetic polymeric drug carriers: focus on brain biocompatibility.
E. Fournier (2003)
10.1016/S0021-9797(03)00252-2
Formation of intermolecular beta-sheet structures: a phenomenon relevant to protein film structure at oil-water interfaces of emulsions.
T. Lefèvre (2003)
10.1111/j.1600-065X.1974.tb01547.x
The Subunit Structure of Transplantation Antigens
L. Rask (1974)
10.1046/J.1523-1755.1999.00491.X
Hypothesis: is renal allograft rejection initiated by the response to injury sustained during the transplant process?
C. Lu (1999)
Biocompatibility and tissue regenerating capacity of crosslinked dermal sheep collagen
B. P. (2003)
10.1021/BM010082Z
Elaboration and characterization of whey protein beads by an emulsification/cold gelation process: application for the protection of retinol.
L. Beaulieu (2002)
10.1055/S-2004-821215
Use of an artificial dermis (Integra) for the reconstruction of extensive burn scars in children. About 22 grafts.
N. Groos (2005)
10.1097/00007890-199608150-00003
Grafting on nude mice of living skin equivalents produced using human collagens.
C. A. López Valle (1996)
10.1016/S0305-4179(03)00013-5
Skin replacement with a collagen based dermal substitute, autologous keratinocytes and fibroblasts in burn trauma.
D. Wisser (2003)
Biologic attachment, growth, and differentiation of cultured human epidermal keratinocytes on a graftable collagen and chondroitin-6-sulfate substrate.
S. Boyce (1988)
10.1097/00007890-199505000-00001
ALLOGENEIC‐SYNGENEIC CULTURED EPITHELIA: A SUCCESSFUL THERAPEUTIC OPTION FOR SKIN REGENERATION
M. Rouabhia (1995)
10.1002/BIP.10423
Molecular mechanisms of Fe2+‐induced β‐lactoglobulin cold gelation
G. Remondetto (2003)
Molecular mechanisms of Fe 2 + - induced β - lactoglobulin cold gelation : an interaction story
G Remondetto (2003)
10.1016/J.CCELL.2003.09.003
Burn wound assessment and surgical management.
J. F. Bishop (2004)
Characterization and evaluation of whey protein-based biofilms as substrates for in vitro cell cultures Biomaterials
V Gilbert (2005)
10.1016/S0094-1298(03)00075-0
Skin tissue engineering.
H. Bannasch (2003)
10.1002/1097-0282(200012)54:7<578::AID-BIP100>3.0.CO;2-2
Molecular differences in the formation and structure of fine-stranded and particulate beta-lactoglobulin gels.
T. Lefèvre (2000)
10.1021/JF00040A001
Sorbitol- vs Glycerol-Plasticized Whey Protein Edible Films: Integrated Oxygen Permeability and Tensile Property Evaluation
T. Mchugh (1994)
10.1002/(SICI)1097-4636(19980905)41:3<443::AID-JBM14>3.0.CO;2-J
Comparative histological evaluation of new tyrosine-derived polymers and poly (L-lactic acid) as a function of polymer degradation.
K. A. Hooper (1998)
Elaboration and characterization of whey protein beads by an emulsification/cold gelation process: application for the protection of retinol Biomacromolecules
L Beaulieu (2002)
10.1002/(SICI)1097-4636(199707)36:1<65::AID-JBM8>3.0.CO;2-J
Development of degradable polyesterurethanes for medical applications: in vitro and in vivo evaluations.
B. Saad (1997)
10.1016/S0305-4179(00)00045-0
Artificial skin, split-thickness autograft and cultured autologous keratinocytes combined to treat a severe burn injury of 93% of TBSA.
M. Loss (2000)
Whey protein biofilms: new non-toxic substrates for biomedical S43
M Subirade (2001)
10.1023/B:CATB.0000034082.29214.3d
Applied tissue engineering in the closure of severe burns and chronic wounds using cultured human autologous keratinocytes in a natural fibrin matrix
J. Kopp (2004)
Use of a composite skin graft composed of cultured human keratinocytes and fibroblasts and a collagen-GAG matrix to cover full-thickness wounds on athymic mice.
M. L. Cooper (1991)
10.1021/JF040286H
Iron availability from whey protein hydrogels: an in vitro study.
G. Remondetto (2004)
Permanent skin replacement using engineered epidermis containing fewer than 5% syngeneic keratinocytes.
F. Larochelle (1998)
10.1097/00007890-199605150-00002
Permanent skin replacement using chimeric epithelial cultured sheets comprising xenogeneic and syngeneic keratinocytes.
M. Rouabhia (1996)
Molecular differences in the formation and structure of fine-stranded and particulate β-lactoglobulin gels Biopolymers
T Lefèvre (2000)
10.1007/s004230100227
Organotypical engineering of differentiated composite-skin equivalents of human keratinocytes in a collagen-GAG matrix (INTEGRA Artificial Skin) in a perfusion culture system
M. Kremer (2001)
10.1097/00129334-200101000-00016
Using skin replacement products to treat burns and wounds.
S. L. Hansen (2001)
10.1111/J.1365-2621.1994.TB06980.X
Plasticized Whey Protein Edible Films: Water Vapor Permeability Properties
T. Mchugh (1994)
10.1016/J.BURNS.2005.08.015
The use of artificial dermis for reconstruction of full thickness scalp burn involving the calvaria.
E. Yeong (2006)
Bioartificial skin Cells Tissues Organs
H GMachens (2000)
10.1002/(SICI)1097-4636(20000605)50:3<397::AID-JBM14>3.0.CO;2-A
In vivo biocompatibility of dextran-based hydrogels.
J. Cadée (2000)
10.1097/01.BCR.0000124749.85552.CD
A review of keratinocyte delivery to the wound bed.
D. Chester (2004)
10.1097/00007890-199505000-00002
MIXED CULTURES COMPRISING SYNGENEIC AND ALLOGENEIC MOUSE KERATINOCYTES AS A GRAFTABLE SKIN SUBSTITUTE
T. Suzuki (1995)
Leukocytosis: basics of clinical assessment.
N. Abramson (2000)
10.1001/JAMA.1989.03430150093032
Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate.
J. Hansbrough (1989)
10.1016/0168-3659(94)00109-8
Drug-polymer interaction affecting the mechanical properties, adhesion strength and release kinetics of piroxicam-loaded Eudragit E films plasticized with different plasticizers
S. Lin (1995)
10.1023/A:1015850301214
The Effect of Plasticizers on Compatibility, Mechanical Properties, and Adhesion Strength of Drug-Free Eudragit E Films
S. Lin (2004)



This paper is referenced by
10.1007/978-1-4939-6595-3_7
β-Lactoglobulin-Based Nano and Microparticulate Systems for the Protection and Delivery of Bioactives
Fatoumata Diarrassouba (2017)
10.1016/j.biomaterials.2009.04.003
Biocompatibility of injectable chitosan-phospholipid implant systems.
Raquel de Souza (2009)
10.1002/jbm.a.36040
Zein-based composites in biomedical applications.
Merve Demir (2017)
Designing a Whey Protein Based Material as a Scaffold for Bone Regeneration
Mia Dvora (2010)
10.1039/C5RA25506F
Single step synthesized sulfur and nitrogen doped carbon nanodots from whey protein: nanoprobes for longterm cell tracking crossing the barrier of photo-toxicity
Bodhisatwa Das (2016)
Impact of Chemotherapy Dosing Schedule on Ovarian Cancer Tumor Responsiveness
R. D. Souza (2012)
10.1021/bm2000378
Biobased poly(propylene sebacate) as shape memory polymer with tunable switching temperature for potential biomedical applications.
B. Guo (2011)
Preparation and Partial Characterization of Sago Starch Based Graft Co-Polymers
Santhanam Sekar (2015)
10.1002/jbm.a.36754
Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering.
Michal Dziadek (2019)
10.1016/j.ijbiomac.2010.02.011
Structure-function characteristics of the biomaterials based on milk-derived proteins.
A. Ghosh (2010)
10.7282/T30C4VZ9
Design, fabrication and molecular modeling of protein subunits for use in a novel hydrogel
C. Gaughan (2009)
Interactions between ß-lactoglobulin and nutraceutical ligands riboflavin, vitamin D₃ and lysozyme : formation, physico-chemical and biological characterization of functional delivery scaffolds
Fatoumata Diarrassouba (2013)
Semantic Scholar Logo Some data provided by SemanticScholar