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Chondrogenic Differentiation Of Human Embryonic Stem Cell-derived Cells In Arginine-glycine-aspartate-modified Hydrogels.

Nathaniel S Hwang, Shyni Varghese, Zijun Zhang, Jennifer H Elisseeff
Published 2006 · Biology, Medicine
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Human embryonic stem cells (hESCs) have the potential to self-renew and generate multiple cell types, producing critical building blocks for tissue engineering and regenerative medicine applications. Here, we describe the efficient derivation and chondrogenic differentiation of mesenchymal-like cells from hESCs. These cells exhibit mesenchymal stem cell (MSC) surface markers, including CD29, CD44, CD105, and platelet-derived growth factor receptor-alpha. Under appropriate growth conditions, the hESC-derived cells proliferated without phenotypic changes and maintained MSC surface markers. The chondrogenic capacity of the cells was studied in pellet culture and after encapsulation in poly(ethylene glycol)-diacrylate (PEGDA) hydrogels with exogenous extracellular proteins or arginineglycine- aspartate (RGD)-modified PEGDA hydrogels. The hESC-derived cells exhibited growth factor- dependent matrix production in pellet culture but did not produce tissue characteristic of cartilage morphology. In PEGDA hydrogels containing exogenous hyaluronic acid or type I collagen, no significant cell growth or matrix production was observed. In contrast, when these cells were encapsulated in RGDmodified poly(ethylene glycol)hydrogels, neocartilage with basophilic extracellular matrix deposition was observed within 3 weeks of culture, producing cartilage-specific gene up-regulation and extracellular matrix production. Our results indicate that precursor cells characteristic of a MSC population can be cultured from differentiating hESCs through embryoid bodies, thus holding great promise for a potentially unlimited source of cells for cartilage tissue engineering.



This paper is referenced by
10.1007/s10856-018-6203-9
Sustained release of TGF-β1 via genetically-modified cells induces the chondrogenic differentiation of mesenchymal stem cells encapsulated in alginate sulfate hydrogels
M. Askari (2018)
10.1016/j.actbio.2017.11.020
Electrospun thermosensitive hydrogel scaffold for enhanced chondrogenesis of human mesenchymal stem cells.
Alexander Robert Brunelle (2018)
10.1016/j.carbpol.2018.06.016
Hyaluronate-alginate hybrid hydrogels modified with biomimetic peptides for controlling the chondrocyte phenotype.
Hyoseok An (2018)
10.1002/term.1683
Chondrogenic potential of injectable κ-carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue-engineering applications.
Elena Geta Popa (2015)
10.1533/9780857099037.3.219
Cartilage grafts for bone repair and regeneration
Chelsea S. Bahney (2014)
10.1007/978-3-7091-0385-2_10
Cartilage Engineering: Current Status and Future Trends
Emily E. Coates (2011)
10.1016/j.biomaterials.2010.03.065
The enhancement of human embryonic stem cell osteogenic differentiation with nano-fibrous scaffolding.
Laura A. Smith (2010)
10.1073/pnas.0809680106
In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells
Nathaniel S Hwang (2008)
10.1016/j.biomaterials.2019.05.021
Collagen microencapsulation recapitulates mesenchymal condensation and potentiates chondrogenesis of human mesenchymal stem cells - A matrix-driven in vitro model of early skeletogenesis.
Yuk Yin Li (2019)
10.1002/jbm.a.36208
RGD-functionalized polyethylene glycol hydrogels support proliferation and in vitro chondrogenesis of human periosteum-derived cells.
Abhijith Kundadka Kudva (2018)
10.1016/J.POLYMER.2012.03.064
Synthetic substrates for long-term stem cell culture
Aftin M. Ross (2012)
10.1201/b11625-44
Matrices for zonal cartilage tissue engineering
Dino Irawan (2012)
10.1002/adma.200904179
Mechanical properties of cellularly responsive hydrogels and their experimental determination.
April M Kloxin (2010)
10.1016/j.biomaterials.2009.08.004
The effects of heparin releasing hydrogels on vascular smooth muscle cell phenotype.
Jeffrey A. Beamish (2009)
3 D PRINTING OF HYALURONIC ACID SCAFFOLDS FOR TISSUE ENGINEERING APPLICATIONS
(2013)
10.1155/2019/9671206
Immunomodulatory Functions of Mesenchymal Stem Cells in Tissue Engineering
Haojiang Li (2019)
10.1089/ten.tea.2008.0320
Development of serum-free, chemically defined conditions for human embryonic stem cell-derived fibrochondrogenesis.
Eugene J. Koay (2009)
Modulation of connective tissue stromal cell plasticity to generate cartilaginous phenotypes
Iris K Cheung (2010)
10.1002/marc.200900818
Injectable Biodegradable Poly(ethylene glycol)/RGD Peptide Hybrid Hydrogels for in vitro Chondrogenesis of Human Mesenchymal Stem Cells.
Shao Qiong Liu (2010)
Three dimensional glycosaminoglycan mimetic peptide amphiphile hydrogels for regenerative medicine applications
Yasin Tümtaş (2015)
The effects of applied hydrostatic pressure on osteogenic differentiation of mouse mesenchymal stem cells in an in vitro 3D culture
Brittany Ho McGowan (2014)
10.1242/jcs.006064
Extracellular matrix dynamics in development and regenerative medicine
William P. Daley (2008)
10.1007/5584_2018_175
Embryonic Stem Cells in Development and Regenerative Medicine.
Ayşegül Doğan (2018)
10.1089/ten.TEB.2012.0757
Generating cartilage repair from pluripotent stem cells.
Aixin Cheng (2014)
10.1089/ten.TEA.2010.0097
Response of human embryonic stem cell-derived mesenchymal stem cells to osteogenic factors and architectures of materials during in vitro osteogenesis.
Jiang Lei Hu (2010)
10.1016/j.biologicals.2017.04.003
Collagen-alginate microspheres as a 3D culture system for mouse embryonic stem cells differentiation to primordial germ cells.
Vahid Mansouri (2017)
10.1517/14712598.2016.1145651
Current clinical evidence for the use of mesenchymal stem cells in articular cartilage repair
Dimitris Reissis (2016)
10.1115/1.3127262
Mechanical characterization of differentiated human embryonic stem cells.
Gidon Ofek (2009)
10.1016/j.biomaterials.2009.04.038
The influence of RGD-bearing hydrogels on the re-expression of contractile vascular smooth muscle cell phenotype.
Jeffrey A. Beamish (2009)
10.1533/9780857098887.2.238
Biomimetic hydrogels as scaffolds for tissue-engineering applications
Jie-fang Zhu (2013)
10.1016/j.copbio.2009.10.005
Stem cells in musculoskeletal engineered tissue.
Tara L. Deans (2009)
10.4161/org.6.3.12419
Advances in musculoskeletal tissue engineering
Carlo Alberto Rossi (2010)
See more
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