Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

The Biotin-(strept)avidin System: Principles And Applications In Biotechnology.

E. Diamandis, T. K. Christopoulos
Published 1991 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The biotin-(strept)avidin system has been used for many years in a variety of different applications. Here we present a general overview of the system, describe its components and advantages, and show how the system is used in various applications, with emphasis on immunological and nucleic acid hybridization assays. This system is now considered a versatile independent technology with broad applications in many branches of biotechnology. Clearly, its use will continue to grow in the years to come.
This paper references
10.1093/NAR/15.11.4513
Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA.
G. Gebeyehu (1987)
10.1016/0003-2697(90)90136-W
Direct quantitation of biotin-labeled nucleotide analogs in RNA transcripts.
B. Fenn (1990)
10.1080/07328318708056219
Synthesis Of Oligodeoxyribonucleotides Containing an Aliphatic Amino Linker Arm at Selected Adenine Bases and Derivatization with Biotin
J. Roduit (1987)
10.1016/0165-022X(86)90022-9
An improved method for the single-step purification of streptavidin.
E. Bayer (1986)
10.1042/BJ2480167
Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.
Y. Hiller (1987)
10.1016/0009-9120(88)90006-9
A new europium chelate for protein labelling and time-resolved fluorometric applications.
R. Evangelista (1988)
J Immunol Methods
(1989)
10.1038/328429A0
Host origin of marrow stromal cells following allogeneic bone marrow transplantation
P. Simmons (1987)
10.1016/0076-6879(90)84283-M
Purification of gonadotropin-releasing hormone receptors.
E. Hazum (1990)
10.1016/0009-8981(90)90302-9
Analytical methodology for immunoassays and DNA hybridization assays--current status and selected systems--critical review.
E. Diamandis (1990)
MethodsEnzymol
(1990)
10.1016/S0009-9120(88)80083-3
Time-resolved fluoroimmunoassay of cortisol in serum with a europium chelate as label.
E. Diamandis (1988)
Use of avidin-biotin complex in an ELISA system: a quantitative comparison with two other immunoperoxidase detection systems using keratin antisera.
J. Madri (1983)
10.1021/AC00216A021
Streptavidin-based macromolecular complex labeled with a europium chelator suitable for time-resolved fluorescence immunoassay applications
R. Morton (1990)
10.1073/PNAS.77.8.4666
Iminobiotin affinity columns and their application to retrieval of streptavidin.
K. Hofmann (1980)
Immunomagnetic removal of B-lymphoma cells using a novel mono-sized magnetizable polymer bead, M-280, in conjunction with primary IgM and IgG antibodies.
G. Kvalheim (1989)
10.1073/PNAS.81.7.1991
Genomic sequencing.
G. Church (1984)
10.1016/0076-6879(93)18038-E
Magnetic DNA affinity purification of yeast transcription factor.
O. Gabrielsen (1993)
10.1073/PNAS.82.9.2593
A chemically cleavable biotinylated nucleotide: usefulness in the recovery of protein-DNA complexes from avidin affinity columns.
M. Shimkus (1985)
10.1093/NAR/13.3.745
Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.
A. Forster (1985)
10.1016/0009-9120(90)90221-F
Detection techniques for immunoassay and DNA probing applications.
E. Diamandis (1990)
10.1080/10408348708542802
Time-Resolved Fluorescence of Lanthanide Probes and Applications in Biotechnology
E. Soini (1987)
10.1093/NAR/14.15.6115
Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.
E. Jablonski (1986)
10.1126/SCIENCE.3051381
DNA diagnostics--molecular techniques and automation.
U. Landegren (1988)
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures
S. Hus (1981)
Methodsof enzymology
M Wilchek (1990)
10.1038/338599A0
Chemiluminescence lights up
I. Bronstein (1989)
10.1042/BJ1010774
Thermodynamics of the binding of biotin and some analogues by avidin.
N. Green (1966)
10.1128/JCM.23.2.311-317.1986
Novel chemical method for the preparation of nucleic acids for nonisotopic hybridization.
R. Viscidi (1986)
10.1016/0009-9120(90)90238-P
Novel and sensitive noncompetitive (two-site) immunoassay for haptens with emphasis on peptides.
E. Ishikawa (1990)
10.1093/NAR/18.15.4632
An easy method to check the efficiency of biotin end-labelling of DNA-fragments.
K. Holmstrom (1990)
10.1093/NAR/7.6.1485
Specific chemical labeling of DNA fragments.
H. Eshaghpour (1979)
10.1177/36.6.3259249
Histological detection of messenger RNAs with biotinylated synthetic oligonucleotide probes.
A. F. Guitteny (1988)
10.1073/PNAS.68.10.2604
Irreversible inhibition of biotin transport in yeast by biotinyl-p-nitrophenyl ester.
J. Becker (1971)
10.1016/0006-291X(87)90305-6
Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA.
A. Reisfeld (1987)
10.1016/0076-6879(90)84260-N
[6] Nonglycosylated avidin
Y. Hiller (1990)
10.1073/PNAS.83.24.9591
Rapid plasmid library screening using RecA-coated biotinylated probes.
B. Rigas (1986)
10.1093/NAR/14.4.1871
Molecular cloning and nucleotide sequence of the streptavidin gene.
C. Argaraña (1986)
10.1021/AC00176A011
Multiple fluorescence labeling with europium chelators. Application to time-resolved fluoroimmunoassays.
E. Diamandis (1989)
10.1093/NAR/11.18.6167
Biotin and fluorescent labeling of RNA using T4 RNA ligase.
R. W. Richardson (1983)
10.1016/0076-6879(90)84326-C
Colorimetric-detected DNA sequencing.
S. Beck (1990)
10.1016/0022-1759(80)90337-3
Detection of attograms of antigen by a high-sensitivity enzyme-linked immunoabsorbent assay (HS-ELISA) using a fluorogenic substrate.
A. Shalev (1980)
10.1093/NAR/16.9.4077
Synthesis and hybridization of a series of biotinylated oligonucleotides.
A. Cook (1988)
Glycolipid tumor cell markers and their monoclonal antibodies: drug targeting and immunosuppression
Hakomori (1982)
10.1038/316542a0
Immunocytochemical localization in rat brain of a prolactin release-inhibiting sequence of gonadotropin-releasing hormone prohormone
H. Phillips (1985)
10.1177/34.5.2939131
Immunocytochemical binding of anti-opsin N-terminal-specific antibodies to the extracellular surface of rod outer segment plasma membranes. Fixation induces antibody binding.
A. Polans (1986)
10.1021/ja00479a048
Avidin-biotin affinity columns. General methods for attaching biotin to peptides and proteins
K. Hofmann (1978)
Improved enzyme immunoassays using biotin-avidin-enzyme complex.
A. Shamsuddin (1983)
10.1093/NAR/14.15.6227
Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.
S. Agrawal (1986)
10.1073/PNAS.83.9.2934
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
D. Pinkel (1986)
10.1093/NAR/18.15.4355
A protected biotin containing deoxycytidine building block for solid phase synthesis of biotinylated oligonucleotides.
U. Pieles (1990)
10.1093/NAR/15.12.4857
Preparation of base-modified nucleosides suitable for non-radioactive label attachment and their incorporation into synthetic oligodeoxyribonucleotides.
J. Haralambidis (1987)
Egg white avidin
RJ DeLange (1971)
10.1016/0022-1759(87)90120-7
Labeling of the oligosaccharide moieties of immunoglobulins.
D. O'shannessy (1987)
10.1016/0003-9861(82)90325-3
A reagent for covalently attaching biotin to proteins via a cleavable connector arm.
C. Mouton (1982)
10.1016/S0021-9673(01)93733-1
Application of avidin-biotin technology to affinity-based separations.
E. Bayer (1990)
10.1177/27.8.90074
The use of avidin-biotin interaction in immunoenzymatic techniques.
J. L. Guesdon (1979)
10.1016/0003-2697(87)90105-9
Photobiotin as a sensitive probe for protein labeling.
E. Lacey (1987)
Time-resolved fluorescence of lanthanide probesand applications in biotechnology [Review)
E Soini (1987)
10.1016/S0009-9120(89)80065-7
Sensitive time-resolved immunofluorometric assay of thyrotropin in serum.
E. Reichstein (1989)
Preparation of digoxigenin-labeled probes by the polymerase chain reaction.
J. Lanzillo (1990)
Protein biotinylation.
E. Bayer (1990)
The synthesis of oligonucleotides containing an aliphatic CLINICALCHEMISTRY
LM Smith (1991)
10.1016/0003-2697(81)90456-5
Purification of avidin and its derivatives on 2-iminobiotin-6-aminohexyl-Sepharose 4B.
G. Orr (1986)
10.1016/0022-1759(89)90104-X
Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.
M. Bobrow (1989)
10.1002/9780470110461.CH1
The use of the avidin-biotin complex as a tool in molecular biology.
E. Bayer (1980)
10.1093/CLINCHEM/35.3.491
Time-resolved fluorescence immunoassay system especially suited for research applications.
E. Diamandis (1989)
10.1038/310792A0
Monoclonal antibody production by receptor-mediated electrically induced cell fusion
M. M. Lo (1984)
10.1093/CLINCHEM/34.9.1790
Nonisotopic "sandwich" immunoassay of thyroglobulin in serum by the biotin-streptavidin technique: evaluation and comparison with an immunoradiometric assay.
C. M. Preissner (1988)
10.1093/CLINCHEM/33.11.1994
Immunofluorometry of choriogonadotropin by time-resolved fluorescence spectroscopy, with a new europium chelate as label.
M. Khosravi (1987)
New assay configurations for competitive-type time- resolvedfluoroimmunoassays of haptens [Abstract]
A Papanastasiou-Diamandi (1989)
10.1093/NAR/15.8.3595
Molecular cloning of the chicken avidin cDNA.
M. Gope (1987)
10.1016/0076-6879(90)84262-F
Isolation and properties of streptavidin.
E. Bayer (1990)
amino group at the 5 ’ terminus : synthesis of fluorescent DNA primers for use in DNA sequence analysis
AF Cook
Investigations of avidin and biotin for imaging applications.
D. Hnatowich (1987)
10.1038/340733A0
Magnetic separation of DNA
M. Uhlén (1989)
Reversible biotinylation of Clq with a cleavable biotinylated derivative. Application in Clq receptor (ClqR)purification
B Ghebrehiwet (1988)
10.1093/CLINCHEM/35.5.804
Immunoradiometric assay of corticotropin with use of avidin-biotin separation.
R. Zahradnik (1989)
10.1016/0076-6879(90)84271-H
2-Iminobiotin-containing reagent and affinity columns.
B. Fudem-Goldin (1990)
10.1016/0003-2697(87)90370-8
Detection of biotinylated DNA probes by using Eu-labeled streptavidin and time-resolved fluorometry.
P. Dahlén (1987)
10.1073/PNAS.78.11.6633
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
P. R. Langer (1981)
Chemiluminescence lights up.Nature (London) 1989;338:599-600
I Bronstein (1989)
Proc NatI Acad Sci
(1980)
10.1093/NAR/18.15.4345
Biotinyl and phosphotyrosinyl phosphoramidite derivatives useful in the incorporation of multiple reporter groups on synthetic oligonucleotides.
K. Misiura (1990)
10.1016/0022-1759(88)90031-2
Time-resolved fluorescence using a europium chelate of 4,7-bis-(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA). Labeling procedures and applications in immunoassays.
E. Diamandis (1988)
Aviclin [Review
MN Green (1975)
10.1016/0003-2697(87)90123-0
The use of biotinylated monoclonal antibodies and streptavidin affinity chromatography to isolate herpesvirus hydrophobic proteins or glycoproteins.
D. Gretch (1987)
Time - resolved fluorescence of lanthanide probes and applications in biotechnology [ Review )
E Soini (1989)
10.1093/NAR/5.2.363
Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels.
T. Broker (1978)
New assay configurations for competitive-type timeresolvedfluoroimmunoassays of haptens [Abstract
A Papanastasiou-Diamandi (1989)
10.1016/0378-1119(82)90032-4
Sensitive detection of RNA using strand-specific M13 probes.
D. Brown (1982)
10.1093/CLINCHEM/32.10.1873
Two-monoclonal-antibody sandwich-type assay for thyrotropin, with use of an avidin-biotin separation technique.
W. O'Dell (1986)
10.1016/s0075-7535(08)x7008-1
Practice and theory of enzyme immunoassays
P. Tijssen (1985)
10.1177/34.12.3023477
A single-step silver enhancement method permitting rapid diagnosis of cytomegalovirus infection in formalin-fixed, paraffin-embedded tissue sections by in situ hybridization and immunoperoxidase detection.
D. Przepiorka (1986)
10.1002/j.1460-2075.1984.tb02230.x
DNA sequencing with direct blotting electrophoresis.
S. Beck (1984)
General methods for attaching biotin to pepticdes and proteins
(1978)
10.1016/0165-2478(84)90008-7
A novel procedure for labeling immunoglobulins by conjugation to oligosaccharide moieties.
D. O'shannessy (1984)
10.1093/NAR/17.19.7643
Synthesis and use of labelled nucleoside phosphoramidite building blocks bearing a reporter group: biotinyl, dinitrophenyl, pyrenyl and dansyl.
A. Roget (1989)
10.1016/0076-6879(90)84268-L
[14] Protein biotinylation
E. Bayer (1990)
10.1016/0042-6822(83)90460-9
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.
D. Brigati (1983)
10.1016/0167-5699(84)90027-6
The avidin-biotin complex in immunology.
M. Wilchek (1984)
10.1002/JPS.2600780803
Digoxin immunoassay with monoclonal and polyclonal antibodies using time-resolved fluorometry.
A. Papanastasiou-Diamandi (1989)
10.1021/AC00161A024
Laser-excited time-resolved solid-phase fluoroimmunoassays with the new europium chelate 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid as label.
E. Reichstein (1988)
Ann Clin Lab Sci
(1985)
10.1016/0003-2697(83)90418-9
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Andrew P. Feinberg (1983)
10.1016/0003-2697(88)90120-0
The avidin-biotin complex in bioanalytical applications.
M. Wilchek (1988)
Improved enzyme immunoassays using biotin-avidin-enzyme complex.
Shamsuddin Am (1983)
10.1021/BI00625A005
Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA.
S. Isaacs (1977)
10.1093/CLINCHEM/35.7.1441
Chemiluminescent assay of alkaline phosphatase applied in an ultrasensitive enzyme immunoassay of thyrotropin.
I. Bronstein (1989)
10.1016/0076-6879(90)84267-K
Biotin-containing reagents.
M. Wilchek (1990)
10.1016/S0003-2670(00)80734-5
Homogeneous enzyme-linked competitive binding assay for biotin based on the avidin-biotin interaction
S. Daunert (1988)
10.1093/NAR/17.13.4937
Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support.
T. Hultman (1989)
DNA probes
GH Keller (1989)
peptide hormones: structural analysis and applications to flowcytometmy
W Newman (1991)
10.1073/PNAS.83.23.9085
Use of nonisotopic M13 probes for genetic analysis: application to HLA class II loci.
E. Sheldon (1986)
Measurement of serum chorionic gonadotropin by a biotin-avidin labeled enzyme immunoassay.
E. Fehskens (1985)
10.1073/PNAS.71.9.3537
Use of the avidin-biotin complex for specific staining of biological membranes in electron microscopy.
H. Heitzmann (1974)
Avidin-biotin affinity columns
K Hofinann



This paper is referenced by
10.1002/path.1525
Western blot analysis of a limited number of cells: a valuable adjunct to proteome analysis of paraffin wax‐embedded, alcohol‐fixed tissue after laser capture microdissection
W. Martinet (2004)
10.1080/15321819408009573
Sensitivity of immunoenzymometric assay and detection method of enzyme.
G. Kominami (1994)
10.1007/978-3-319-96172-9_3
Application of NOEs and 3 J HH -Couplings in 3D Structure Determination
C. Hoeck (2018)
10.1002/prca.200900217
Multiplex assays for biomarker research and clinical application: Translational science coming of age
Qin Fu (2010)
10.1201/9780203908976.PT2
Biomaterials Synthetic and Engineering Strategies
S. Cannizzaro (2002)
10.1111/febs.14685
Crystal structure of afifavidin reveals common features of molecular assemblage in the bacterial dimeric avidins
O. Avraham (2018)
10.1002/PSSA.201800030
A Novel N‐Substituted Pyrrole Based Surface Modification for Biosensing
Fabian Rüger (2018)
10.1210/jc.2016-1971
Factitious Graves' Disease Due to Biotin Immunoassay Interference-A Case and Review of the Literature.
M. Elston (2016)
10.1098/rstb.2018.0167
mRNAs biotinylated within the 5′ cap and protected against decapping: new tools to capture RNA–protein complexes
S. Bednarek (2018)
10.3390/molecules24112036
Biotin-Avidin-Mediated Capture of Microspheres on Polymer Fibers
L. Ahrens (2019)
10.1016/J.MENCOM.2015.11.020
High-resolution mass spectra of biotinylated, HEG-spacered molecular probes with oligosaccharide fragments of the capsular polysaccharides from Streptococcus pneumoniae
A. Chizhov (2015)
10.1006/ABIO.2002.5724
Bacterial expression of in vivo-biotinylated aequorin for direct application to bioluminometric hybridization assays.
M. Verhaegen (2002)
10.1007/978-0-387-98063-8_8
Label-Free Biochemical Sensors Based on Optical Microresonators
Chung-Yen Chao (2009)
10.1021/bc200276k
Detection of cellular sialic acid content using nitrobenzoxadiazole carbonyl-reactive chromophores.
Jessie A. Key (2012)
10.1039/C3SC51363G
Cu(I)-catalysed N–H insertion in water: a new tool for chemical biology
K. Tishinov (2013)
10.1007/978-1-59745-579-4_6
Functionality screen of streptavidin mutants by non-denaturing SDS-PAGE using biotin-4-fluorescein.
Nicolas Humbert (2008)
TWO-DIMENSIONAL FLUIDIZATION OF NANOMATERIALS VIA BIOMIMETIC MEMBRANES TOWARDS ASSISTED SELF-ASSEMBLY
K. Kelly (2017)
10.1039/c6an00348f
Approaches towards molecular amplification for sensing.
S. Goggins (2016)
10.1016/j.ecl.2017.04.002
Biochemical Testing in Thyroid Disorders.
N. Esfandiari (2017)
10.1021/acs.analchem.5b03999
Highly Sensitive Two-Dimensional Paper Network Incorporating Biotin-Streptavidin for the Detection of Malaria.
Benjamin D Grant (2016)
10.1093/CLINCHEM/41.4.564
6 alpha-biotinylated estrone: novel tracer in competitive chemiluminescence immunoassay of estrone in serum.
P. Luppa (1995)
10.1016/j.toxicon.2008.12.020
Synthesis of bifunctional saxitoxin analogues by biotinylation.
C. Robillot (2009)
10.1515/cclm-2017-0059
A simple method to detect biotin interference on immunoassays
L. Lam (2017)
10.1039/b922525k
A kit for the investigation of live Escherichia coli cell adhesion to glycosylated surfaces.
M. Hartmann (2010)
DEVELOPMENT OF FUNCTIONAL PROTEOMICS APPROACHES FOR STUDYING RETROGRADE TRANSPORT
Shi Getao (2011)
10.5772/INTECHOPEN.82072
Detection Systems in Immunohistochemistry
S. Shojaeian (2018)
10.1021/acsomega.8b02559
Star-like Supramolecular Complexes of Reducing-End-Functionalized Cellulose Nanocrystals
Ana Villares (2018)
10.1039/c3cc48640k
Streptavidin binding as a model to characterize thiol-ene chemistry-based polyamine surfaces for reversible photonic protein biosensing.
E. Melnik (2014)
10.1016/j.bbagen.2015.03.009
Streamlined method for parallel identification of single domain antibodies to membrane receptors on whole cells.
M. Rossotti (2015)
Survivin expression in childhood medulloblastomas: a possible diagnostic and prognostic marker.
B. Bodey (2004)
10.1016/S0022-1759(96)00173-1
Preferential labeling of alpha-amino N-terminal groups in peptides by biotin: application to the detection of specific anti-peptide antibodies by enzyme immunoassays.
I. Sélo (1996)
10.21608/ADJALEXU.2017.57929
EXPRESSION OF METASTASIS ASSOCIATED PROTEIN 1 AS A POSSIBLE PROGNOSTIC INDICATOR IN ORAL SQUAMOUS CELL CARCINOMA
W. M. Nawar (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar