Online citations, reference lists, and bibliographies.
← Back to Search

Experimental Chlamydia Trachomatis Infection Causes Apoptosis In Human Sperm.

A. Satta, A. Stivala, A. Garozzo, A. Morello, A. Perdichizzi, E. Vicari, M. Salmeri, A. Calogero
Published 2006 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
BACKGROUND Chlamydia trachomatis is responsible for a widespread sexually transmitted infection. In men, it is associated with a wide clinical spectrum causing infertility. Furthermore, C. trachomatis serovar E infection decreases motility and increases the number of non-viable sperm. No other effects of C. trachomatis have been reported on sperm despite the crucial role of DNA integrity for sperm function. The aim of this study was to investigate the effects of C. trachomatis on sperm apoptosis. METHODS Sperm from eight normozoospermic men were incubated with increasing concentrations of C. trachomatis serovar E elementary bodies (EB) for 6 and 24 h. Sperm were then collected to evaluate phosphatidylserine (PS) membrane translocation and DNA fragmentation by Annexin V-propidium iodide staining, TUNEL assay and flow cytometry. RESULTS After 6 h of incubation, C. trachomatis had no effect on the percentage of sperm showing PS externalization. However, a significant effect on this parameter was observed after 24 h. C. trachomatis also significantly increased the number of sperm with DNA fragmentation both after 6 and 24 h of incubation. CONCLUSIONS C. trachomatis causes sperm PS externalization and DNA fragmentation. These effects may explain the negative direct impact of C. trachomatis infection on sperm fertilizing ability.
This paper references
10.1128/jcm.10.3.351-356.1979
Reticulate bodies as single antigen in Chlamydia trachomatis serology with microimmunofluorescence
E. C. Yong (1979)
10.1128/jcm.10.2.259-261.1979
Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test
S. Wang (1979)
10.1016/0163-4453(92)91944-7
Male genital Chlamydia trachomatis infections.
J. Oriel (1992)
10.1128/jcm.33.12.3186-3190.1995
Sensitivities of PCR, MicroTrak, ChlamydiaEIA, IDEIA, and PACE 2 for purified Chlamydia trachomatis elementary bodies in urine, peripheral blood, peripheral blood leukocytes, and synovial fluid
J. Kuipers (1995)
10.1128/iai.65.11.4539-4547.1997
Response of Chlamydia trachomatis serovar E to iron restriction in vitro and evidence for iron-regulated chlamydial proteins
J. Raulston (1997)
10.1128/CMR.10.1.160
Current methods of laboratory diagnosis of Chlamydia trachomatis infections
C. Black (1997)
10.1093/HUMUPD/5.5.433
Chlamydia trachomatis: impact on human reproduction.
J. Paavonen (1999)
10.1093/HUMREP/14.4.1039
Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic.
D. Evenson (1999)
10.1530/JRF.0.1150001
The Amoroso Lecture. The human spermatozoon--a cell in crisis?
R. Aitken (1999)
10.1128/IAI.68.9.4872-4876.2000
Coincubation of Human Spermatozoa with Chlamydia trachomatis In Vitro Causes Increased Tyrosine Phosphorylation of Sperm Proteins
S. Hosseinzadeh (2000)
10.1002/J.1939-4640.2000.TB03272.X
World Health Organization Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 4th ed.
D. Lamb (2000)
10.1016/S0015-0282(99)00462-8
Sperm chromatin damage impairs human fertility
M. Spanô (2000)
Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team.
M. Spanô (2000)
10.1530/REP.0.1220497
Oxidative stress, DNA damage and the Y chromosome.
R. Aitken (2001)
10.1016/S0090-4295(01)01180-3
Biologic variability of sperm DNA denaturation in infertile men.
A. Zini (2001)
10.1093/HUMREP/16.2.293
Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death.
S. Hosseinzadeh (2001)
Globozoospermia is associated with chromatin structure abnormalities
E. Vicari (2002)
10.1099/JMM.0.04836-0
Chlamydia trachomatis-induced death of human spermatozoa is caused primarily by lipopolysaccharide.
S. Hosseinzadeh (2003)
Chlamydia trachomatis and sperm lipid peroxidation in infertile men.
A. Segnini (2003)
10.1002/J.1939-4640.2004.TB02764.X
Semen quality of men with asymptomatic chlamydial infection.
Saeid Hosseinzadeh (2004)
10.1111/j.0001-6349.2004.00542.x
Chlamydiae in the ejaculate: their influence on the quality and morphology of sperm
Z. Veznik (2004)
Sperm DNA damage and its clinical relevance in assessing reproductive outcome.
R. Sharma (2004)
10.1016/S1472-6483(10)61104-2
Inhibition of oocyte fertilization by assisted reproductive techniques and increased sperm DNA fragmentation in the presence of Candida albicans: a case report.
N. Burrello (2004)
10.1007/s10495-005-6065-8
Bacteria induce expression of apoptosis in human spermatozoa
J. Villegas (2005)
10.1016/S1473-3099(04)01254-X
Can Chlamydia trachomatis directly damage your sperm?
A. Eley (2005)



This paper is referenced by
10.1016/j.theriogenology.2021.03.016
The effect of Chlamydia infection on koala (Phascolarctos cinereus) semen quality.
L. Hulse (2021)
10.1016/j.theriogenology.2020.05.033
The occurrence and pathology of chlamydiosis in the male reproductive tract of non-human mammals: A review.
S. Pagliarani (2020)
10.1016/j.lfs.2020.117891
Effects of bacteria on male fertility: Spermatogenesis and sperm function.
H. Oghbaei (2020)
10.7589/2019-03-062
EPIDEMIOLOGY OF CHLAMYDIA-INDUCED REPRODUCTIVE DISEASE IN MALE KOALAS (PHASCOLARCTOS CINEREUS) FROM SOUTHEAST QUEENSLAND, AUSTRALIA AS ASSESSED FROM PENILE URETHRAL SWABS AND SEMEN
L. Hulse (2020)
10.1016/j.jri.2020.103183
Bacterial infection of the male reproductive system causing infertility.
M. Farsimadan (2020)
10.3390/microorganisms8010136
Waddlia chondrophila and Male Infertility
D. Baud (2020)
10.1016/j.anireprosci.2020.106683
Dehydration of llama sperm using different osmolarity media and temperatures for preservation.
M. Carretero (2020)
10.1111/andr.12625
Semen quality is affected by HLA class I alleles together with sexually transmitted diseases
P. I. Marques (2019)
10.4081/gh.2019.812
Residential mobility impacts relative risk estimates of space-time clusters of chlamydia in Kalamazoo County, Michigan.
Claudio Owusu (2019)
10.5812/JJM.84954
Investigating Chlamydia trachomatis and Genital Mycoplasma Prevalence and Apoptosis Markers in Infertile and Fertile Couples
M. Moosavian (2019)
10.1186/s12866-019-1392-z
Interaction of different Chlamydiae species with bovine spermatozoa
T. Eckert (2019)
10.1016/j.purol.2019.08.261
[Prevalence and role of IgG anti-Chlamydia trachomatis in a population of infertile men in Morocco].
M. Frikh (2019)
10.1038/s41598-019-42396-3
Growth kinetics of Chlamydia trachomatis in primary human Sertoli cells
S. Filardo (2019)
10.1007/s00105-018-4300-9
Sexuell übertragbare Infektionen und männliche Fertilität
P. Spornraft-Ragaller (2018)
10.1177/0956462417735245
The impact of Chlamydia trachomatis infection on sperm parameters and male fertility: A comprehensive study
M. Moazenchi (2018)
Clinical manifestation of chlamydial urethritis , epididymitis , prostatitis
Young-Suk Lee (2018)
10.1016/j.nmni.2018.08.012
Male infertility: the intracellular bacterial hypothesis
M. Stojanov (2018)
10.1038/s41598-017-12316-4
Erratum: Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
Jenniffer Puerta Suárez (2017)
10.1007/978-981-10-4017-7_12
Sexually Transmitted Infections and Male Infertility: Old Enigma, New Insights
B. Kushwaha (2017)
10.1111/and.12774
The presence of human papillomavirus in semen does not affect the integrity of sperm DNA
E. I. Cortés-Gutiérrez (2017)
10.1038/s41598-017-01262-w
Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
Jenniffer Puerta Suárez (2017)
Gamete proteomic profile of male patients suffering from sexually transmitted infections
M. Flint (2016)
10.1016/J.JEVS.2015.01.014
Control Methods and Evaluation of Bacterial Growth on Fresh and Cooled Stallion Semen
C. R. Neto (2015)
10.1016/J.MEFS.2014.04.003
Transient exposure to Chlamydia trachomatis can induce alteration of sperm function which cannot be stopped by sperm washing
Najwa Al-Mously (2015)
10.5603/fhc.a2015.0019
Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen.
M. Fraczek (2015)
10.1007/s10815-015-0462-x
Can apoptosis and necrosis coexist in ejaculated human spermatozoa during in vitro semen bacterial infection?
M. Fraczek (2015)
10.1016/j.fertnstert.2014.06.002
Fertilizing potential of ejaculated human spermatozoa during in vitro semen bacterial infection.
M. Fraczek (2014)
Study the relation between Chlamydia trachomatis infection and poor semen quality that cause infertility in Iraqi male
Farah Th. Abdullah (2014)
10.3389/fimmu.2014.00534
The Role of the Immune Response in Chlamydia trachomatis Infection of the Male Genital Tract: A Double-Edged Sword
K. Redgrove (2014)
10.1038/nrurol.2014.285
Male infertility: a public health issue caused by sexually transmitted pathogens
F. Gimenes (2014)
10.1155/2014/307950
Chlamydial Infection and Its Role in Male Infertility
M. Samplaski (2014)
10.1371/journal.pone.0098903
Molecular Detection of Chlamydia trachomatis and Other Sexually Transmitted Bacteria in Semen of Male Partners of Infertile Couples in Tunisia: The Effect on Semen Parameters and Spermatozoa Apoptosis Markers
Hanen Sellami (2014)
See more
Semantic Scholar Logo Some data provided by SemanticScholar