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Pulmonary Toxicity Of Single-wall Carbon Nanotubes In Mice 7 And 90 Days After Intratracheal Instillation.
C. Lam, J. James, R. McCluskey, R. Hunter
Published 2004 · Biology, Medicine
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Nanomaterials are part of an industrial revolution to develop lightweight but strong materials for a variety of purposes. Single-wall carbon nanotubes are an important member of this class of materials. They structurally resemble rolled-up graphite sheets, usually with one end capped; individually they are about 1 nm in diameter and several microns long, but they often pack tightly together to form rods or ropes of microscopic sizes. Carbon nanotubes possess unique electrical, mechanical, and thermal properties and have many potential applications in the electronics, computer, and aerospace industries. Unprocessed nanotubes are very light and could become airborne and potentially reach the lungs. Because the toxicity of nanotubes in the lung is not known, their pulmonary toxicity was investigated. The three products studied were made by different methods and contained different types and amounts of residual catalytic metals. Mice were intratracheally instilled with 0, 0.1, or 0.5 mg of carbon nanotubes, a carbon black negative control, or a quartz positive control and euthanized 7 d or 90 d after the single treatment for histopathological study of the lungs. All nanotube products induced dose-dependent epithelioid granulomas and, in some cases, interstitial inflammation in the animals of the 7-d groups. These lesions persisted and were more pronounced in the 90-d groups; the lungs of some animals also revealed peribronchial inflammation and necrosis that had extended into the alveolar septa. The lungs of mice treated with carbon black were normal, whereas those treated with high-dose quartz revealed mild to moderate inflammation. These results show that, for the test conditions described here and on an equal-weight basis, if carbon nanotubes reach the lungs, they are much more toxic than carbon black and can be more toxic than quartz, which is considered a serious occupational health hazard in chronic inhalation exposures.
This paper references
PULMONARY TOXICITY OF SIMULATED LUNAR AND MARTIAN DUSTS IN MICE: I. HISTOPATHOLOGY 7 AND 90 DAYS AFTER INTRATRACHEAL INSTILLATION
C. Lam (2002)
Roll up for the revolution
P. Ball (2001)
Taming high - tech particles . Cautious steps into the nanotech future
R. Hanoa (2002)
Experimental silicosis. II. Long-term effects of intratracheally instilled quartz on collagen metabolism and morphologic characteristics of rat lungs.
K. Reiser (1983)
Evaluation of aerosol release during the handling of unrefined single walled carbon nanotube material
P. A. Baron (2003)
Nature of exposure to chrysotile.
M. Lippman (1994)
Rodent health monitoring summary
Charles River (1998)
Determinants of the pathogenicity of man-made vitreous fibers (MMVF)
G. Oberdörster (2000)
Graphite pneumoconiosis of electrotypers.
E. Gaensler (1966)
J. Gilman (2001)
Nanometre-size tubes of carbon
P. Ajayan (1997)
Comparative inhalation toxicity of nickel subsulfide to F344/N rats and B6C3F1 mice exposed for 12 days.
J. Benson (1987)
Comparative inhalation toxicity of nickel sulfate to F344/N rats and B6C3F1 mice exposed for twelve days.
J. Benson (1988)
Carbon Nanotubes. From Macromolecules to Nanotechnology
P. Ajayan (2000)
Thirteen-week inhalation toxicity study with aerosol mixtures of fog oil and graphite particles in F344/N male rats. Prepared by IIT Research Institute
C Aranyi (1992)
Documentation of the Threshold Limit Values and Biological Exposure Indices
B. Derubska (1964)
PULMONARY TOXICITY OF SIMULATED LUNAR AND MARTIAN DUSTS IN MICE: II. BIOMARKERS OF ACUTE RESPONSES AFTER INTRATRACHEAL INSTILLATION
C. Lam (2002)
NTP Technical Report on Toxicity Studies of Cadmium Oxide (CAS No. 1306–19–0) Administered by Inhalation to F344/N Rats and B6C3F1 Mice
Atypical quartz dust-induced pneumoconiosis in SPF rats
K. Eden (2004)
Inhalation toxicity of single materials and mixtures. Phase 4. Thirteen-week inhalation toxicity study with aerosol mixtures of fog oil and graphite particles in f344/n male rates. Final report, September 1991-July 1992
C. Aranyi (1992)
Deposition and retention models for internal dosimetry of the human respiratory tract. Task group on lung dynamics.
Bates Dv (1966)
Buckytube MSDS. Carbon Nanotechnologies Incorporated
In-plane-aligned membranes of carbon nanotubes
D. Walters (2001)
Intratracheal instillation as an exposure technique for the evaluation of respiratory tract toxicity: uses and limitations.
K. Driscoll (2000)
Toxicity of Military Smokes and Obscurants. National Research Council Subcommittee on Military Smokes and Obscurants
Experimental silicosis. I. Acute effects of intratracheally instilled quartz on collagen metabolism and morphologic characteristics of rat lungs.
K. Reiser (1982)
NANOMATERIALS: SAFE OR UNSAFE?
Ron Dagani (2003)
Taming high-tech particles
J. Gorman (2002)
Large-scale purification of single-wall carbon nanotubes: process, product, and characterization
A. G. Rinzler (1998)
Comparative biology of the normal lung
R. A. Parent (1992)
Synthetic graphite. National Institute for Occupational Safety and Health
Niosh Osha (1988)
Nature of exposure to chrysotile.
M. Lippmann (1994)
NTP Technical Report on Toxicity Studies of Cadmium Oxide (CAS No
Focus on carbon nanotubes
P. Ball (1999)
Taming high-tech particles. Cautious steps into the nanotech future
J Gorman (200)
Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats.
D. Warheit (2004)
Exposure to Carbon Nanotube Material: Aerosol Release During the Handling of Unrefined Single-Walled Carbon Nanotube Material
A. Maynard (2004)
Production and measurements of individual single-wall nanotubes and small ropes of carbon
S. Arepalli (2001)
Graphite pneumoconiosis in carbon electrode makers
H. Okutani (1964)
Evaluation of aerosol release during the handling of unrefined single walled carbon nanotube material. NIOSH DART-02-191
P A Baron (2003)
NATIONAL NANOTECHNOLOGY INITIATIVE - LEADING TO THE NEXT INDUSTRIAL REVOLUTION
Duncan T. Moore (2000)
Gas-phase production of carbon single-walled nanotubes from carbon monoxide via the HiPco process: A parametric study
M. Bronikowski (2001)
Graphite pneumoconiosis. A review of etiologic and epidemiologic aspects.
R. Hanoa (1983)
Nanotechnology. An interview with Dr. Richard Smalley. ISI Essential Science Indicators Special Topics
Physiological and pathophysiological pulmonary responses to inhaled nuisance‐like or fibrogenic dusts
D. Warheit (1991)
Toxicity of Military Smokes and Obscurants
Associates carry research forward in form of nanotube. Rice News 6
L Unrau (1996)
Rodent health monitoring summary. Charles River Laboratories
Charles River (1998)
Comparative pathological aspects of chronic olivine and silica inhalation in mice.
T. Wilson (1986)
R. McClellan (1997)
Associates carry research forward in form of nanotube
L. Unrau (1996)
Deposition and retention models for internal dosimetry of the human respiratory tract. Health Phys
D V Bates (1966)
Quantitative morphometric analysis of pulmonary deposition of aerosol particles inhaled via intratracheal nebulization, intratracheal instillation or nose‐only inhalation in rats
B. K. Leong (1998)
This paper is referenced by
TB research at UT-Houston--a review of cord factor: new approaches to drugs, vaccines and the pathogenesis of tuberculosis.
R. Hunter (2009)
The Determination of the Potential Risks Associated with Engineered Nanoparticles in Aquatic Environments
K. Carey (2015)
Instructions for use Title Toxicity evaluations of various carbon nanomaterials
Uo Motohiro (2017)
Particle shape effects in vitro and in vivo.
Bradley J. Harris (2012)
Nanotechnology Stewardship : Executive Report Canadian Stewardship Practices for Environmental Nanotechnology March 2005
S. Bergeron (2005)
Adsorption of monoaromatic compounds and pharmaceutical antibiotics on carbon nanotubes activated by KOH etching.
L. Ji (2010)
Inhaled Carbon Nanotubes Reach the Sub-Pleural Tissue in Mice
Jessica P. Ryman-Rasmussen (2009)
Nanotoxicology—A Pathologist’s Perspective
Ann F. Hubbs (2011)
Effect of Fiber Length on Carbon Nanotube-Induced Fibrogenesis
A. Manke (2014)
Nanotechnology Startup Concerns, Information Needs, and Opportunities to Proactively Address Environmental, Health, and Social Issues Focus on firms in Connecticut and New York
Deanna N. Lekas (2006)
DAPI Method: A Novel Assay to Evaluate the In vitro Impact of Nanomaterials on Mammalian Cells
P. V. Raja (2006)
Nanotechnology and Nanotoxicology
J. Curtis (2006)
Identification of Systemic Markers from A Pulmonary Carbon Nanotube Exposure
A. Erdely (2011)
Simulation of the subsurface mobility of carbon nanoparticles at the field scale
E. Cullen (2010)
Genotoxicity, cytotoxicity, and reactive oxygen species induced by single‐walled carbon nanotubes and C60 fullerenes in the FE1‐Muta™Mouse lung epithelial cells
Nicklas Raun Jacobsen (2008)
Nanoparticle Interactions with Biological Systems and Subsequent Activation of Intracellular Signaling Mechanisms
V. Stone (2007)
Toxicity of Carbon Nanotubes and Its Implications for Occupational and Environmental Health
C. Lam (2007)
Sustainable governance of emerging technologies—Critical constellations in the agent network of nanotechnology
A. Wiek (2007)
Comparative study of pulmonary responses to nano- and submicron-sized ferric oxide in rats.
Mo-Tao Zhu (2008)
Studying the potential release of carbon nanotubes throughout the application life cycle
A. Köhler (2008)
Inhalation toxicity of multiwall carbon nanotubes in rats exposed for 3 months.
L. Ma-Hock (2009)
Nanotechnologie - eine Bestandsaufnahme
H. Krug (2007)
Potential Applications of Carbon Nanotubes
M. Endo (2007)
Impact of carbon nanotube exposure, dosage and aggregation on smooth muscle cells.
P. V. Raja (2007)
Study of morphology of aerosol aggregates formed during co-pyrolysis of C3H8 + Fe(CO)5
N. A. Ivanova (2007)
exchange of concepts, methods and know-how and, therefore, a close collaboration is required between those working in drug delivery and particle toxicology."
Sharif M.I. Hossain (2011)
Nanotechnology: Human Safety Issues, Research Gaps and Potential Beneficial Opportunities⋆
M. Gwinn (2011)
Kolnanorör -Exponering, toxikologi och skyddsåtgärder i arbetsmiljön
P. Gustavsson (2011)
Prospects and applications of nanobiotechnology: a medical perspective
M. Fakruddin (2012)
Nanotechnology and Regulation within the framework of the Precautionary Principle Final Report
U. Petschow (2004)
ESH & Nanotechnology: A Joint Study by the Chemical Industry Vision2020 and the Semiconductor Research Corporation
T. Brady (2006)
ptake , excretion and toxicity of nano-sized latex particles on medaka Oryzias latipes ) embryos and larvae
asahiko Manabea (2011)See more