Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Ligand Assays: From Electrophoresis To Miniaturized Microarrays.

R. Ekins
Published 1998 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
The main developments in the "ligand assay" field in which I have been involved are traced. These include the original development of "first generation" competitive assays relying on radiolabeled analyte markers; the development of the first "second generation", noncompetitive (ultrasensitive) methods, which rely on the use of labeled (monoclonal) antibodies and high specific activity nonisotopic labels (leading to the transformation of the immunodiagnostic field in the 1980s); and the development of the first "third generation" miniaturized, chip-based, microarray methods, which permit the simultaneous ultrasensitive measurement of many analytes in the same small sample. The latter--applicable both to immunoassay and to DNA/RNA analysis--are likely to revolutionize the diagnostic and pharmaceutical fields in the next decade.
This paper references
High-performance assays of small molecules: enhanced sensitivity, rapidity, and convenience demonstrated with a noncompetitive immunometric anti-immune complex assay system for digoxin.
C. Self (1994)
Competitive enzyme-liked immunoassay with use of soluble enzyme/antibody immune complexes for labeling. I. Measurement of human choriogonadotropin.
D. E. Yorde (1976)
inventor. Measurement of analyte concentration
RP Ekins (1983)
Saturation analysis; a microanalytical technique for assaying some compounds of biological importance
RP Ekins (1963)
Mathematical theory of immunoradiometric (labeled antibody) assays.
D. Rodbard (1973)
Measurement of hormones–radioimmunoassay
SA Berson (1973)
Time-resolved fluorometer for lanthanide chelates--a new generation of nonisotopic immunoassays.
E. Soini (1983)
Continuous cultures of fused cells secreting antibody of predefined specificity
G. Köhler (1975)
Light-directed, spatially addressable parallel chemical synthesis.
S. P. Fodor (1991)
General principles of radioimmunoassay Radioisotopes in medicine: in vitro studies. Oak Ridge
Rs Yalow (1968)
Solid-phase antigen-antibody systems
L. Wide (1971)
Assay of vitamin B12 in blood. A simple method.
R. M. Barakat (1961)
Detection of viral antigens by time-resolved fluoroimmunoassay.
P. Halonen (1983)
Theoretical aspects of “saturation” and radioimmunoassay
RP Ekins (1968)
Measurement of free hormones in blood.
R. Ekins (1990)
Clinical Chemistry
D. Seligson (1953)
Physico-chemical methods of detection of a previously unidentified adrenal hormone
SA Simpson (1953)
Labelled Antibodies and Immunological Assay Systems
L. Miles (1968)
The international vocabulary of basic and general terms in metrology
P. Clifford (1985)
Detection of hepatitis B surface antigen using time - resolved fluoroimmunoas
H Siitari (1983)
An immunoradiometric assay of insulin Protein and polypeptide hormones. Part 1
Leh Miles (1968)
Europium as a label in time-resolved immunofluorometric assays.
I. Hemmilä (1984)
An immunoradiometric assay of insulin
Miles LEH (1968)
Measurement of analyte concentration
RP Ekins (1983)
Pulsed light, time resolved fluoroimmunoassay
NJ Marshall (1998)
Point On the meaning of “sensitivity”
R. Ekins (1997)
Infant feeding with SMA. Further observations.
Todd Rm (1965)
Diagnosis of allergy by an in-vitro test for allergen antibodies.
L. Wide (1967)
Time-resolved fluoroimmunoassay: a new test for rubella antibodies.
O. Meurman (1982)
The estimation of thyroxine in human plasma by an electrophoretic technique
Ekins Rp (1960)
Oxford English dictionary
R. Burchfield (1982)
A shadow over immunoassay
R. Ekins (1989)
Monoclonal antibodies and developments in immunoassay Proceedings of the 14th International Leukocyte Culture Conference edited by Alberto Albertini and Roger Ekins, Elsevier/North-Holland, 1981. $03.00/Dfl.148.00 (ix + 402 pages) ISBN 0 444 80373 4.
A. Voller (1982)
Saturation analysis; a microanalytical technique for assaying some compounds of biological importance Radioaktive isotope in klinik und forschung
Rp Ekins (1963)
Multianalyte microspot immunoassay--microanalytical "compact disk" of the future.
R. Ekins (1991)
Immunoassay using antigen—enzyme conjugates
B. van Weemen (1971)
[Principles of nuclear medicine].
D. Emrich (1974)
On the meaning of "sensitivity": a rejoinder.
R. Ekins (1998)
The use of 3 H and 14 C labeled acetic anhydride as analytical reagents in microbiochemistry
P Avivi (1954)
Immunoassay of endogenous plasma insulin in man.
R. Yalow (1960)
Serum TATI levels and clinical correlation in tumors of the head and neck.
J. Rayo (1991)
Immunoassay standardization.
R. Ekins (1991)
On the meaning of "sensitivity".
R. Ekins (1997)
Theoretical aspects of saturation analysis.
R. Ekins (1970)
Extensive polymorphisms observed in HIV–1 clade B protease gene using high–density oligonucleotide arrays
M. Kozal (1996)
New Developments in Diagnostic Virology
P. Bachmann (1983)
Pulsed light, time resolved fluoroimmunoassay Monoclonal antibodies and developments in immunoassay
Nj Marshall (1981)
General principles of radioimmunoassay.
S. Berson (1968)
Radioisotopes in Medicine: In Vitro Studies
M. Loken (1969)
The estimation of thyroxine in human plasma by an electrophoretic technique.
R. Ekins (1960)
Ultrasensitive ligand assays
RP Ekins (1995)
Development of microspot multi-analyte ratiometric immunoassay using dual fluorescent-labelled antibodies
R. Ekins (1989)
Immunoassay using antigenenzyme conjugates
BK VanWeeman (1971)
Continuous culture of fused cells secreting specific antibody of predefined specificity
G Köhler (1975)
Trends in immunoassay technologies
LJ Kricka (1993)
Competitive enzyme-linked immunoassay with the use of soluble enzyme antibody immune complexes for labelling. I. Measurement of human choriogonadotrophin
DE Yorde (1976)
Dr. Pardue responds
H. Pardue (1975)
The use of 3H and 14C labeled acetic anhydride as analytical reagents in microbiochemistry
P Avivi (1954)
Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay
H. Siitari (1983)
Measurement of analyte concentration. British patent 8224600
Rp Ekins (1983)
Ultrasensitive ligand assays Principles of nuclear medicine
Rp Ekins (1995)
Immunoassay using antigenenzyme conjugates
Bk Van Weeman (1971)
Mathematical theory of immunometric (labeled antibody) assay
D Rodbard (1973)
Mono - clonal antibodies and developments in immunoassay
A Albertini (1981)
The inseparable triad: analytical sensitivity, measurement uncertainty, and quantitative resolution.
H. Pardue (1997)
"Homogeneous" enzyme immunoassay. A new immunochemical technique.
K. Rubenstein (1972)
Theoretical aspects of saturation analysis Karolinska symposia on research methods in reproductive endocrinology. 2nd symposium: steroid assay by protein binding
R Ekins (1970)
Time-Resolved Fluorescence of Lanthanide Probes and Applications in Biotechnology
E. Soini (1987)

This paper is referenced by
Bioadhesive nanoareas in antifouling matrix for highly efficient affinity sensors
I. Mannelli (2008)
Development of a biosensor based on linear dichroism spectroscopy
Sandeep Kaur Sandhu (2015)
Microarrays: the reincarnation of multiplexing in laboratory medicine, but now more relevant?
C. P. Price (2001)
Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells
Xingyue An (2017)
Integrated Microfluidic Device for Functional Secretory Immunophenotyping of Immune Cells.
Roberto Rodriguez-Moncayo (2020)
Optimal Design of Microarray Immunoassays to Compensate for Kinetic Limitations
W. Kusnezow (2006)
Competitive Assays of Label-Free DNA Hybridization with Single-Molecule Fluorescence Imaging Detection.
E. Peterson (2016)
Kinetics of protein binding in solid-phase immunoassays: theory.
K. Klenin (2005)
Continuing evolution of in-vitro diagnostic instrumentation
G. Cohn (2000)
OM-CVD on Patterned SAMs
Wolfgang Knoll (2013)
Fast Detection of Low Volumes of Mouse Antibodies Using a Nanofluidic Bioarray Chip
Jonathan S. C. Lee (2013)
Microarray Technology: Transcending Towards New Era of Gene Expression and Its Potential Applications
Virendra Kumar Vishwakarma (2019)
Effects of monosodium-L-glutamate administration on serum levels of reproductive hormones and cholesterol, epididymal sperm reserves and testicular histomorphology of male albino rats.
I. S. Ochiogu (2015)
Clinical measurement of steroid metabolism.
R. Andrew (2001)
Biomarkers quantification with antibody arrays in cancer early detection.
A. Gallotta (2012)
Protein microarrays for antibody profiling: Specificity and affinity determination on a chip
O. Poetz (2005)
Fundamental Investigation of Inkjet Deposition and Physical Immobilization of Horseradish Peroxidase on Cellulosic Substrates
S. D. Risio (2011)
Microarray data analysis: a practical approach for selecting differentially expressed genes
D. M. Mutch (2001)
Development of a receptor-based microplate assay for the detection of beta-lactam antibiotics in different food matrices.
Janine Lamar (2007)
Nanomechanical protein detectors based on the mechanical property measured by nano-gap actuators
W. Lee (2005)
PHOSPHOR TECHNOLOGY : Exceptional Photoluminescent Properties Light Up Homogeneous Bioanalytical Assays by Terhi Riuttamäki ( née Rantanen )
Terhi Rantanen (2011)
Electrochemical Immunoassay with Microscopic Immunomagnetic Bead Domains and Scanning Electrochemical Microscopy
C. Wijayawardhana (2000)
Biochips beyond DNA: technologies and applications.
J. Ng (2003)
MicroRNA profiling using µParaflo microfluidic array technology.
Xiaochuan Zhou (2012)
Review : Advancements and application of immunosensors in the analysis of food contaminants
M. S. Azam (2016)
Biosensing enhancement using passive mixing structures for microarray-based sensors.
N. Lynn (2014)
Dentine extracellular matrix components liberated by calcium silicate cements and their effects on dental pulp cells
P. Tomson (2013)
Polypyrrole based DNA hybridization assays: study of label free detection processes versus fluorescence on microchips.
T. Livache (2003)
Inner ear drug delivery via a reciprocating perfusion system in the guinea pig.
Zhiqiang Chen (2005)
MS‐Binding Assays: Kinetic, Saturation, and Competitive Experiments Based on Quantitation of Bound Marker as Exemplified by the GABA Transporter mGAT1
Christine Zepperitz (2006)
revisã o Fatores Interferentes na Interpretação d e Dosagens Laboratoriais no Diagnóstico d e Hiper e Hipotireoidism o
H. Gra (2002)
Functionalized Polymer Microgel Particles Enable Customizable Production of Label-Free Sensor Arrays.
Mark A. Lifson (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar