Turnera aphrodisiacaWard (Turneraceae) has been traditionally used for the treatment of anxiety neurosis, and as an aphrodisiac. Mother tinctures (85% ethanol extracts) ofT. aphrodisiacahave also been used for the treatment of central nervous system disorders. In the present investigation,T. aphrodisiacamother tinctures formulated by three reputed manufacturers of homoeopathic medicines (NLK, DWSG and SBL) were evaluated for their anxiolytic activity. Dried mother tinctures ofT. aphrodisiacawere subjected to anxiolytic activity evaluation at various doses, i.e. 50, 75, 100, 125 or 150 mg/kg p.o. in mice using elevated plus maze apparatus. Dried mother tinctures exhibited significant anxiolytic activity at 50 mg/kg (NLK), 75 mg/kg (DWSG) and 125 mg/kg (SBL), respectively, with reference to control as well as standard (diazepam, 2 mg/kg p.o.). Mother tinctures ofT. aphrodisiacaavailable in the market, have significant anxiolytic activity. Amongst the three mother tinctures ofT. aphrodisiacaanalyzed, the dry residue of NLK possesses the highest amount of anxiolytic constituent(s). To ensure uniformity and consistency of biological effects in herbal formulations, these should be standardized on the basis of bioactive markers. The authors are actively involved in isolating the bioactive constituent(s) fromT. aphrodisiacaso that the plant can be standardized on the basis of biologically active constituent(s).