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Calretinin And Other Mesothelioma Markers In Synovial Sarcoma: Analysis Of Antigenic Similarities And Differences With Malignant Mesothelioma

M. Miettinen, J. Limon, A. Niezabitowski, J. Lasota
Published 2001 · Medicine, Biology

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Synovial sarcoma (SS) is a mesenchymal neoplasm that typically shows epithelial differentiation. SS commonly metastasizes to lung and pleura, and has also been reported as the primary in these locations. The histologic distinction of SS from mesothelioma may be difficult because of the combination of epithelioid and spindle cells, potentially shared locations, and antigenic expression. In this study the authors examined 103 well-documented SSs including 41 biphasic, 44 monophasic, and 18 poorly differentiated SSs in comparison with 23 epithelioid and seven sarcomatous mesotheliomas. Most biphasic SSs (29 of 41, 71%) had fields or foci of calretinin-positive tumor cells. The spindle cell components were more often positive (55%), whereas 14% of tumors had positive epithelial cells. The monophasic and poorly differentiated SSs commonly had foci of calretinin-positive cells (in 52% and 56% of cases respectively). In comparison, all 23 epithelioid mesotheliomas (EM) were extensively calretinin positive and seven sarcomatoid mesotheliomas were variably calretinin positive. HBME-1 positivity was similarly detected in biphasic SS and EM (100% and 87% respectively). Among the other sarcomas, two of 15 malignant peripheral nerve sheath tumors were focally calretinin positive, whereas 16 epithelioid sarcomas, 20 leiomyosarcomas, 20 gastrointestinal stromal tumors, and 20 angiosarcomas were negative. Biphasic SSs differed from mesotheliomas by their more common BerEp4 positivity (90%) whereas EMs showed focal reactivity in 13% cases. Marked CD15 reactivity was rare in both. Wilms tumor protein-1 (WT1) was not detected in SS, but was present in 12 of 17 EMs. CD141 was rare in SS, limited to spindle cell components, whereas EMs typically showed prominent membrane staining in epithelial cells. Simple epithelial keratins were present in all epithelial cells of biphasic SS and mesothelioma (keratin 7[K7], K19), but were only focal in monophasic and poorly differentiated SS. Biphasic SSs were extensively K14 positive (89% of cases), whereas epithelial and sarcomatoid mesotheliomas typically showed only scattered positive cells. The potentially shared calretinin patterns in SS and mesothelioma require the use of other markers. The discriminating features include extensive BerEp4 positivity, rarity of CD141, and lack of WT1 in SS. Global expression of K7 and K19 in mesotheliomas versus focal expression in monophasic and poorly differentiated SSs, and differential patterns of K14 expression may also be helpful.
This paper references
10.1097/00000478-199906000-00010
Selective distribution of calretinin in adenocarcinomas of the human colon and adjacent tissues.
V. Gotzos (1999)
10.1097/00000478-199609000-00001
Calretinin: a novel immunocytochemical marker for mesothelioma.
C. Doglioni (1996)
Detection of the SYT-SSX fusion transcripts in formaldehyde-fixed, paraffin-embedded tissue: a reverse transcription polymerase chain reaction amplification assay useful in the diagnosis of synovial sarcoma.
J. Lasota (1998)
10.1007/s004280000238
Patterns of keratin polypeptides in 110 biphasic, monophasic, and poorly differentiated synovial sarcomas
M. Miettinen (2000)
Value of calretinin immunostaining in differentiating epithelial mesothelioma from lung adenocarcinoma.
N. Ordóñez (1998)
10.1097/00000478-199810000-00006
Value of cytokeratin 5/6 immunostaining in distinguishing epithelial mesothelioma of the pleura from lung adenocarcinoma.
N. Ordóñez (1998)
10.1046/j.1365-2559.1999.00630.x
Primary synovial sarcoma of the lung: report of two cases confirmed by molecular detection of SYT‐SSX fusion gene transcripts
Hisaoka (1999)
10.1016/0092-8674(82)90400-7
The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells
R. Moll (1982)
Wilms' tumor 1 susceptibility (WT1) gene products are selectively expressed in malignant mesothelioma.
K. Amin (1995)
Molecular diagnosis of synovial sarcoma and characterization of a variant SYT-SSX2 fusion transcript.
I. Fligman (1995)
10.1097/00000478-199108000-00008
Ber‐EP4 Antibody as a Discriminant in the Differential Diagnosis of Malignant Mesothelioma Versus Adenocarcinoma
K. Sheibani (1991)
10.1016/0046-8177(95)90242-2
Primary pulmonary sarcomas with features of monophasic synovial sarcoma: a clinicopathological, immunohistochemical, and ultrastructural study of 25 cases.
H. Zeren (1995)
10.1097/00000478-199712000-00001
Immunophenotyping of malignant mesothelioma.
M. Wick (1997)
Synovial sarcoma: a new approach to the histological classification and comparison with malignant mesothelioma
J Rys (1992)
10.1002/GCC.2870030504
Cytogenetics of Synovial Sarcoma: Presentation of Ten New Cases and Review of the Literature
J. Limon (1991)
10.1097/00125480-199801000-00052
Calretinin: a novel tool for diagnostic immunohistochemistry.
A. D. Dei Tos (1998)
Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: a unifying model
T-T Sun (1985)
10.1046/j.1365-2559.2000.00770.x
HBME‐1, MOC‐31, WT1 and calretinin: an assessment of recently described markers for mesothelioma and adenocarcinoma
Oates (2000)
10.1097/00000478-199712000-00002
The value of antibodies 44-3A6, SM3, HBME-1, and thrombomodulin in differentiating epithelial pleural mesothelioma from lung adenocarcinoma: a comparative study with other commonly used antibodies.
N. Ordóñez (1997)
The intermediate filament cytoskeleton of malignant mesotheliomas and its diagnostic significance.
G. Blobel (1985)
10.1002/1097-0142(19940101)73:1<74::AID-CNCR2820730114>3.0.CO;2-I
Cardiac synovial sarcoma with translocation (X; 18) associated with asbestos exposure
C. M. Karn (1994)
10.1097/00000478-199712000-00003
The immunohistochemical diagnostic panel for epithelial mesothelioma: a reevaluation after heat-induced epitope retrieval.
J. R. Riera (1997)
10.1093/AJCP/105.2.195
Primary pulmonary sarcoma with morphologic features of monophasic synovial sarcoma and chromosome translocation t(X; 18).
M. Kaplan (1996)
10.1016/S0344-0338(11)80708-9
Immunohistochemical profile of synovial sarcoma with emphasis on the epithelial-type differentiation. A study of 49 primary tumours, recurrences and metastases.
J. Lopes (1994)
10.1007/BF02890064
Expression of keratin 5 as a distinctive feature of epithelial and biphasic mesotheliomas
R. Moll (1989)
The calcium - binding protein calretinin 22 k , an alternative splicing product of the calretinin gene is expressed in several colon adenocarcinoma cell lines
V Gotzos (1996)
Calretinin--sensor or buffer--function still unclear.
K. Billing-Marczak (1999)
Cytogenetics of synovial M. MIETTINEN ET AL
J Limon (2001)
Thrombomodulin expression in malignant pleural mesothelioma and pulmonary adenocarcinoma.
C. L. Collins (1992)
10.1002/1097-0142(19940801)74:3<986::aid-cncr2820740332>3.0.co;2-a
Cardiac synovial sarcoma with translocation (X; 18) associated with asbestos exposure
F. Le Marc'Hadour (1994)
10.1016/S0046-8177(84)80284-1
Keratin proteins and carcinoembryonic antigen in synovial sarcomas: an immunohistochemical study of 24 cases.
J. Corson (1984)
10.1016/0046-8177(90)90036-5
Malignant epithelioid pleural mesothelioma versus peripheral pulmonary adenocarcinoma: a histochemical, ultrastructural, and immunohistologic study of 103 cases.
M. Wick (1990)
10.1046/j.1365-2559.2000.00940.x
Calretinin expression in ameloblastomas
M. Altini (2000)
10.1097/00000478-198906000-00005
A Biphasic Tumor of the Mediastinum with Features of Synovial Sarcoma: A Report of Four Cases
G. Witkin (1989)
10.1111/j.1365-2559.1996.tb01393.x
Mesothelioma‐binding antibodies: thrombomodulin, OV 632 and HBME‐1 and their use in the diagnosis of malignant mesothelioma
R. Attanoos (1996)
Primary synovial sarcoma of the lung: report of two cases confirmed by molecular detection of SYT-SSX fusion gene transcript
M Hisaoka (1999)
The calciumbinding protein calretinin 22k, an alternative splicing product of the calretinin gene is expressed in several colon adenocarcinoma cell lines. Cell Calcium 1996;20:63–72
JC Gander (1996)
10.1038/modpathol.3880018
Value of the Mesothelium-Associated Antibodies Thrombomodulin, Cytokeratin 5/6, Calretinin, and CD44H in Distinguishing Epithelioid Pleural Mesothelioma from Adenocarcinoma Metastatic to the Pleura
P. Cury (2000)
10.1016/S0143-4160(96)90051-2
The calcium-binding protein calretinin-22k, an alternative splicing product of the calretinin gene is expressed in several colon adeno carcinoma cell lines.
J. Gander (1996)
10.1046/j.1365-2559.1998.00565.x
Synovial sarcoma of the pleura and its differentiation from other primary pleural tumours: a clinicopathological and immunohistochemical review of three cases
Nicholson (1998)



This paper is referenced by
10.1078/0344-0338-00503
Critical commentary to: Primary poorly differentiated monophasic synovial sarcoma of the lung.
L. Bégin (2003)
10.1007/978-3-030-36438-0_31
Mesenchymal Tumors of the Mediastinum
A. Weissferdt (2020)
10.1097/PAI.0b013e318187dd26
Comparison of Different Clones (WT49 Versus 6F-H2) of WT-1 Antibodies for Immunohistochemical Diagnosis of Malignant Pleural Mesothelioma
K. Tsuta (2009)
10.1078/0344-0338-00502
Primary poorly differentiated monophasic synovial sarcoma of the lung. A case report with immunohistochemical and genetic studies.
Y. Mikami (2003)
10.1136/bcr-2020-236255
Pancreatic mesothelial cyst
Charbel Chater (2020)
10.1016/J.HUMPATH.2003.11.013
The diagnostic utility of immunohistochemistry in distinguishing between mesothelioma and renal cell carcinoma: a comparative study.
N. Ordóñez (2004)
10.7556/JAOA.2006.106.12.699
Malignant mesothelioma: a case presentation and review.
Timothy J. Barreiro (2006)
10.1016/J.ANNDIAGPATH.2005.11.007
Mesothelial cyst of the pancreas.
M. Nicolas (2006)
10.1038/modpathol.2009.180
Sarcomatoid mesothelioma: a clinical–pathologic correlation of 326 cases
S. Klebe (2010)
10.1007/s007950200013
Synovial sarcoma arising from the pleura: a case report with ultrastructural and immunohistological studies
H. Hirano (2002)
10.1111/j.1440-1827.2010.02631.x
Primary cardiac synovial sarcoma: A case report and literature review
Y. Yokouchi (2011)
10.1053/J.HUMPATH.2003.07.002
Expression of calretinin and other mesothelioma-related markers in thymic carcinoma and thymoma.
Chin-Chen Pan (2003)
10.1016/J.HUMPATH.2006.08.010
What are the current best immunohistochemical markers for the diagnosis of epithelioid mesothelioma? A review and update.
N. Ordóñez (2007)
10.1292/jvms.14-0053
Cystic Rete Ovarii and Uterine Tube Adenoma in a Rabbit
J. Chambers (2014)
10.1017/cbo9781316167366
Modern Immunohistochemistry by Peiguo Chu
P. Chu (2000)
10.1016/J.CDIP.2006.07.001
Pulmonary and thoracic sarcomas
D. Salter (2006)
10.1038/modpathol.3800543
Mesothelioma with rhabdoid features: an ultrastructural and immunohistochemical study of 10 cases
N. Ordóñez (2006)
10.1016/B978-0-323-44284-8.00021-1
Malignant and Borderline Mesothelial Tumors of the Pleura
M. R. Wick (2011)
10.21037/jtd.2017.10.125
The pathological and molecular diagnosis of malignant pleural mesothelioma: a literature review.
G. Alí (2018)
10.1080/00313020802579250
Diagnosis of epithelial mesothelioma using tree‐based regression analysis and a minimal panel of antibodies
S. Klebe (2009)
10.4103/0971-5916.200903
Morphological spectrum of mediastinal lesions with special emphasis on evaluation of needle biopsy: An experience from a tertiary care hospital
Riti Aggarwal (2016)
10.1043/0003-9985(2003)127<85:PPS>2.0.CO;2
Primary pleural synovial sarcoma. A case report and review of the literature.
S. B. Ng (2003)
10.5858/arpa.2010-0604-RA
Immunohistochemistry and molecular diagnostics of pleural malignant mesothelioma.
P. Betta (2012)
10.1007/978-3-540-30760-0_12
Pathologic characterization and differential diagnosis of malignant peritoneal mesothelioma.
N. Ordóñez (2007)
10.1136/jcp.2005.029652
My approach to the diagnosis of mesothelial lesions
K. Butnor (2006)
10.1136/jcp.56.3.221
Inhibin is more specific than calretinin as an immunohistochemical marker for differentiating sarcomatoid granulosa cell tumour of the ovary from other spindle cell neoplasms
V. Shah (2003)
10.1016/j.pathol.2018.06.001
Pleural malignant mesothelioma versus pleuropulmonary synovial sarcoma: a clinicopathological study of 22 cases with molecular analysis and survival data.
S. Klebe (2018)
10.1007/s00428-009-0776-0
Soft tissue sarcomas with non-EWS translocations: molecular genetic features and pathologic and clinical correlations
C. Fisher (2009)
10.1016/J.HUMPATH.2005.11.006
Calretinin expression in tumors of adipose tissue.
J. Cates (2006)
10.1007/0-387-28274-2_37
Pitfalls in the Diagnosis of Malignant Mesothelioma
D. Guinee (2005)
10.1016/j.humpath.2011.07.012
Inhibin-α and synaptophysin immunoreactivity in synovial sarcoma with granular cell features.
N. Setsu (2012)
10.1186/2162-3619-3-12
Expression and diagnostic values of calretinin and CK5/6 in cholangiocarcinoma
L. Zhang (2014)
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