← Back to Search
Polymorphisms In CD14, Mannose-binding Lectin, And Toll-like Receptor-2 Are Associated With Increased Prevalence Of Infection In Critically Ill Adults*
Ainsley M. Sutherland, K. Walley, J. Russell
Published 2005 · Medicine
Save to my Library
Download via 🐼 PaperPanda Download via oaDOI Download via OAB Download via LibKey Download via Google Google ScholarAnalyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Objective:To test for the association of single nucleotide polymorphisms of the innate immunity receptors cluster of differentiation (CD)-14, mannose-binding lectin, and Toll-like receptor-2 with clinical phenotype in critically ill patients with systemic inflammatory response syndrome. Design:Genetic association study. Setting:Tertiary care mixed medical-surgery intensive care unit at St. Paul's Hospital, Vancouver, BC, a teaching hospital associated with the University of British Columbia. Patients:A cohort of 252 critically ill Caucasians with systemic inflammatory response syndrome. Interventions:DNA was extracted from discarded blood. Clinical data were gathered by retrospective chart review. Measurements and Main Results:C-159T CD14, the X/Y and B, C, and D polymorphisms of mannose-binding lectin, and T-16933A Toll-like receptor-2 were genotyped using polymerase chain reaction-restriction fragment length polymorphism. We tested for association of genotype with prevalence of positive bacterial cultures, type of organism (Gram-positive, Gram-negative, other), sepsis and septic shock at admission to the intensive care unit, and 28-day survival. CD14 −159TT was associated with increased prevalence of positive bacterial cultures and with Gram-negative bacteria. Mannose-binding lectin haplotype pairs XO/O and O/O were also associated with increased prevalence of positive bacterial cultures but not with a specific organism class. Toll-like receptor-2 −16933AA was associated with increased prevalence of sepsis and with Gram-positive bacteria. In contrast, the polymorphisms were not associated with increased prevalence of septic shock or altered 28-day survival. Conclusions:Single nucleotide polymorphisms in CD14, mannose-binding lectin, and Toll-like receptor-2 are associated with increased prevalence of positive bacterial cultures and sepsis but not with altered prevalence of septic shock or decreased 28-day survival. Furthermore, CD14 single nucleotide polymorphisms were associated with Gram-negative bacteria and Toll-like receptor-2 with Gram-positive bacteria, whereas mannose-binding lectin was not associated with a particular organism class. Thus, single nucleotide polymorphisms in innate immunity receptors may alter recognition and clearance of bacteria without changing outcomes of critically ill adults with systemic inflammatory response syndrome.
This paper references
APACHE II: A severity of disease classification system
E. Draper (1985)
The human mannose-binding protein functions as an opsonin
M. Kuhlman (1989)
CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein.
S. Wright (1990)
Molecular basis of opsonic defect in immunodeficient children
M. Sumiya (1991)
The APACHE III prognostic system. Risk prediction of hospital mortality for critically ill hospitalized adults.
W. Knaus (1991)
High frequencies in African and non-African populations of independent mutations in the mannose binding protein gene.
R. Lipscombe (1992)
Performing the exact test of Hardy-Weinberg proportion for multiple alleles.
S. Guo (1992)
Release from a human monocyte‐like cell line of two different soluble forms of the lipopolysaccharide receptor, CD14
M. Labéta (1993)
Transgenic mice expressing human CD14 are hypersensitive to lipopolysaccharide.
E. Ferrero (1993)
Trimeric structure of a C-type mannose-binding protein.
W. Weis (1994)
Binding of mannan‐binding protein to various bacterial pathogens of meningitis
L. Emmerik (1994)
Interplay between promoter and structural gene variants control basal serum level of mannan-binding protein.
H. Madsen (1995)
Binding of host collectins to the pathogenic yeast Cryptococcus neoformans: human surfactant protein D acts as an agglutinin for acapsular yeast cells
S. Schelenz (1995)
Increased circulating soluble CD14 is associated with high mortality in gram-negative septic shock.
R. Landmann (1995)
Mannose binding protein is involved in first-line host defence: evidence from transgenic mice.
P. Tabona (1995)
The sepsis syndrome. Definition and general approach to management.
R. Bone (1996)
Increased serum concentration of soluble CD14 is a prognostic marker in gram-positive sepsis.
H. Burgmann (1996)
Genetic susceptibility to mycobacteria and other infectious pathogens in humans.
R. Bellamy (1998)
A Polymorphism* in the 5' flanking region of the CD14 gene is associated with circulating soluble CD14 levels and with total serum immunoglobulin E.
M. Baldini (1999)
Toll-like Receptor-4 Mediates Lipopolysaccharide-induced Signal Transduction*
J. Chow (1999)
Molecular defects in variant forms of mannose-binding protein associated with immunodeficiency.
R. Wallis (1999)
Association of TNF2, a TNF-alpha promoter polymorphism, with septic shock susceptibility and mortality: a multicenter study.
J. Mira (1999)
Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors.
H. Brightbill (1999)
C(-260)-->T polymorphism in the promoter of the CD14 monocyte receptor gene as a risk factor for myocardial infarction.
J. Hubáček (1999)
Toll-like Receptor 2 Functions as a Pattern Recognition Receptor for Diverse Bacterial Products*
Egil Lien (1999)
Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2.
A. Aliprantis (1999)
Detection of structural gene mutations and promoter polymorphisms in the mannan-binding lectin (MBL) gene by polymerase chain reaction with sequence-specific primers.
R. Steffensen (2000)
An interleukin-1 genotype is associated with fatal outcome of meningococcal disease.
R. Read (2000)
Effect of CD14 Blockade in Rabbits with Escherichia coli Pneumonia and Sepsis1
Charles W. Frevert (2000)
Mannose binding lectin (MBL) genotype distributions with relation to serum levels in UK Caucasoids.
D. Crosdale (2000)
A Novel Polymorphism in the Toll-Like Receptor 2 Gene and Its Potential Association with Staphylococcal Infection
E. Lorenz (2000)
tection of structural gene mutations and promoter polymorphisms in the mannan - binding lectin ( MBL ) gene by polymerase chain reaction with sequence - specific prim
DJ Crosdale (2000)
A new statistical method for haplotype reconstruction from population data.
M. Stephens (2001)
Genetic polymorphisms in molecules of innate immunity and susceptibility to infection with Wuchereria bancrofti in South India
E. Choi (2001)
MEGA2: molecular evolutionary genetics analysis software
S. Kumar (2001)
Haplotype tagging for the identification of common disease genes
Gillian C L Johnson (2001)
Association study designs for complex diseases
L. Cardon (2001)
Effect of CD14 promoter polymorphism and H. pylori infection and its clinical outcomes on circulating CD14
J. Karhukorpi (2002)
Tumor Necrosis Factor Gene Polymorphisms, Leukocyte Function, and Sepsis Susceptibility in Blunt Trauma Patients
M. Majetschak (2002)
Association between a genomic polymorphism within the CD14 locus and septic shock susceptibility and mortality rate*
S. Gibot (2002)
Synthetic Lipoteichoic Acid from Staphylococcus aureus Is a Potent Stimulus of Cytokine Release
S. Morath (2002)
The Structure of Haplotype Blocks in the Human Genome
S. Gabriel (2002)
Toll-like receptor 2-deficient mice are highly susceptible to Streptococcus pneumoniae meningitis because of reduced bacterial clearing and enhanced inflammation.
H. Echchannaoui (2002)
The –260 C→T promoter polymorphism of the lipopolysaccharide receptor CD14 and severe sepsis in trauma patients
M. Heesen (2002)
Lipopolysaccharide receptor CD14 polymorphism and risk of stroke in a South-German population
C. Lichy (2002)
A polymorphism in the toll-like receptor 2 is associated with IL-12 production from monocyte in lepromatous leprosy.
T. Kang (2002)
Induction of cytokine production in human T cells and monocytes by highly purified lipoteichoic acid: involvement of Toll-like receptors and CD14.
E. Ellingsen (2002)
Human toll-like receptor 4 mutations but not CD14 polymorphisms are associated with an increased risk of gram-negative infections.
D. Agnese (2002)
TLR 2 . Innate immunity PGA
RU Rats (2002)
Cutting Edge: A Toll-Like Receptor 2 Polymorphism That Is Associated with Lepromatous Leprosy Is Unable to Mediate Mycobacterial Signaling1
P. Bochud (2003)
Effect of anti-CD14 monoclonal antibody on clearance of Escherichia coli bacteremia and endotoxemia
S. Opal (2003)
Association of mannose-binding lectin polymorphisms with sepsis and fatal outcome, in patients with systemic inflammatory response syndrome.
P. Garred (2003)
Association of the CD14 gene –159C polymorphism with progression of IgA nephropathy
H. Yoon (2003)
Toll receptors and pathogen resistance
K. Takeda (2003)
Effect of CD14 blockade on endotoxin-induced acute lung injury in mice.
S. Tasaka (2003)
A new frequent allele is the missing link in the structural polymorphism of the human mannan-binding protein
H. Madsen (2004)
Toll-like receptor 2-deficient mice succumb to Mycobacterium tuberculosis infection.
M. Drennan (2004)
Toll‐like receptor 2‐dependent bacterial sensing does not occur via peptidoglycan recognition
Leonardo H Travassos (2004)
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2
Sonja Lotz (2004)
Increased incidence and severity of the systemic inflammatory response syndrome in patients deficient in mannose-binding lectin
K. J. Fidler (2004)
Genetic susceptibility to infectious diseases.
M. Clementi (2006)
This paper is referenced by
American Academy of Optometry Microbial Keratitis Think Tank
L. Szczotka-Flynn (2021)
Molecular Markers in Diagnosis and Prognosis of Sepsis, Severe Sepsis and Septic Shock
Saad khalaf alrikabi (2020)
Role of Toll-Like Receptors 2 and 4 Genes Polymorphisms in Neonatal Sepsis in a Developing Country: A Pilot Study
Bedewy M. Khaled (2020)
Variabilita v genech pro toll-like receptory a jejich vztah k výskytu parodontálních patogenů u chronické parodontitidy
sv. Anny (2020)
Variability in Toll-like Receptor Genes and Their Relation to Occurrence of Periodontal Pathogens in Chronic Periodontitis
S. Anny (2020)
Variabilita v genech pro toll-like receptory a jejich vztah k výskytu parodontálních patogenů u chronické parodontitidy
Původní práce (2020)
Cannabis Sativa Revisited—Crosstalk between microRNA Expression, Inflammation, Oxidative Stress, and Endocannabinoid Response System in Critically Ill Patients with Sepsis
A. Dinu (2020)
Association of TLR4 and TLR9 gene polymorphisms and haplotypes with cervicitis susceptibility
Alex Chauhan (2019)
Cannabis Sativa Revisited - Crosstalk between microRNAs Expression, Inflammation, Oxidative Stress and Endocannabinoid Response System in Critically Ill Patients with Sepsis
A. Rogobete (2019)
Ispitivanje učestalosti polimorfizama gena za faktor nekroze tumora α, interleuikin 6 i interleukin 1β kod prevremeno rođene dece kao faktora rizika za nastanak sepse
Tatjana J. Varljen (2019)
The Burden of Genetic and Epigenetic Traits in Prematurity
Bruna Ribeiro de Andrade Ramos (2018)
The Burden of Genetic and Epigenetic Traits in Prematurity.
B. Ramos (2018)
Toll‐like receptor 2, 4 and 9 polymorphisms and their association with ICU‐acquired infections in Central Greece
M. Chatzi (2018)
Sepsis Diagnostics in the Era of "Omics" Technologies.
M. Prucha (2018)
Genetics in the Prevention and Treatment of Sepsis
J. Reilly (2017)
Genome-wide association study of sepsis in extremely premature infants
L. Srinivasan (2017)
Melanocortin-1 Receptor Polymorphisms and the Risk of Complicated Sepsis After Trauma: A Candidate Gene Association Study
M. Seaton (2017)
Genetic risk factors for serious infections in inflammatory bowel diseases
Saranya Sasidharan (2017)
Environmental transmission factors and host genetic polymorphisms influencing NTM infection in patients with no overt immunodeficiency
Samuel Halstrom (2017)
Marcadores moleculares en el diagnóstico y pronóstico de sepsis, sepsis grave y choque séptico
Alfredo Prado-Díaz (2017)
Genetic Polymorphisms and Trauma Precision Medicine
W. Gu (2017)
Severe Trauma Complications Prediction by Biomarkers
L. Zeng (2017)
Epidemiology of Sepsis: Current Data and Predictions for the Future
B. Staitieh (2017)
Ancestry informative markers and selected single nucleotide polymorphisms in immunoregulatory genes on preterm labor and preterm premature rupture of membranes: a case control study
B. R. A. Ramos (2016)
Early Prediction of Sepsis Incidence in Critically Ill Patients Using Specific Genetic Polymorphisms
V. David (2016)
TLR2, TLR4 and TLR9 genotypes and haplotypes in the susceptibility to and clinical course of Chlamydia trachomatis infections in Dutch women.
S. Verweij (2016)
Emerging drugs for the treatment of sepsis
N. Heming (2016)
Genome-wide association study of survival from sepsis due to pneumonia: an observational cohort study
A. Rautanen (2015)
Association of TLR1, TLR2, TLR4, TLR6, and TIRAP polymorphisms with disease susceptibility
Mamoona Noreen (2015)
Pharmacological targeting of neutrophilic airway inflammation in COPD
Vandana Gupta (2015)
RevisiónCaracterísticas inmunológicas claves en la fisiopatología de la sepsisKey immunological characteristics in the pathophysiology of sepsis
H. G. Gomez (2015)
Características inmunológicas claves en la fisiopatología de la sepsis
H. G. Gomez (2015)See more