Online citations, reference lists, and bibliographies.
← Back to Search

Hydrophobic Interactions Of The Structural Protein GRP1.8 In The Cell Wall Of Protoxylem Elements.

C. Ringli, G. Hauf, B. Keller
Published 2001 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The glycine-rich structural protein GRP1.8 of French bean (Phaseolus vulgaris) is specifically localized in the modified primary cell walls of protoxylem elements. Continuous deposition of GRP1.8 into the cell walls during elongation growth of the plant suggests that GRP1.8 is part of a repair mechanism to strengthen the protoxylem. In this work, a reporter-protein system was developed to study the interaction of GRP1.8 with the extracellular matrix. Fusion proteins of a highly soluble chitinase with different domains of GRP1.8 were expressed in the vascular tissue of tobacco (Nicotiana tabacum), and the chemical nature of the interaction of these fusion proteins in the cell wall compartment was analyzed. In contrast with chitinase that required only low-salt conditions for complete extraction, the different chitinase/GRP1.8 fusion proteins were completely extracted only by a nonionic or ionic detergent, indicating hydrophobic interactions of GRP1.8. The same interactions were found with the endogenous GRP1.8 in bean hypocotyls. In addition, in vitro experiments indicate that oxidative cross-linking of tyrosines might account for the insolubilization of GRP1.8 observed in later stages of protoxylem development. Our data suggest that GRP1.8 forms a hydrophobic protein-layer in the cell wall of protoxylem vessels.
This paper references
10.1104/PP.91.1.39
Alteration of the physical and chemical structure of the primary cell wall of growth-limited plant cells adapted to osmotic stress.
N. Iraki (1989)
10.1146/ANNUREV.PP.30.060179.002233
Plant Cell Fractionation
P. Quail (1979)
10.1104/PP.94.4.1897
Isolation and characterization of a proline-rich cell wall protein from soybean seedlings.
S. K. Francisco (1990)
10.1007/978-94-010-9641-6
Physiology and Biochemistry of Plant Cell Walls
C. Brett (1990)
10.1016/0022-2836(87)90292-0
Further developments of protein secondary structure prediction using information theory. New parameters and consideration of residue pairs.
J. Gibrat (1987)
10.1046/J.1365-313X.1996.09040477.X
Isolation of pl 4.6 extensin peroxidase from tomato cell suspension cultures and identification of Val-Tyr-Lys as putative intermolecular cross-link site.
L. Schnabelrauch (1996)
10.1073/PNAS.88.22.10362
A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole.
J. M. Neuhaus (1991)
10.1016/0092-8674(92)90530-P
Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: A novel, rapid defense response
D. Bradley (1992)
Structural cellwall proteins
B Keller (1993)
10.1105/tpc.4.7.773
Ultrastructural Localization of a Bean Glycine-Rich Protein in Unlignified Primary Walls of Protoxylem Cells.
U. Ryser (1992)
10.1002/j.1460-2075.1989.tb03510.x
Vascular expression of a bean cell wall glycine‐rich protein–β‐glucuronidase gene fusion in transgenic tobacco
B. Keller (1989)
10.1104/pp.104.2.315
Covalent Cross-Links in the Cell Wall
K. Iiyama (1994)
10.1016/S0921-0423(96)80249-8
Plant cell wall architecture: the role of pectins
M. McCann (1996)
10.1038/323178A0
A gene encoding a novel glycine-rich structural protein of petunia
C. M. Condit (1986)
10.1042/BJ2040449
Isodityrosine, a new cross-linking amino acid from plant cell-wall glycoprotein.
S. Fry (1982)
Structural cellwall proteins in protoxylem development : evidence for a repair process mediated by a glycinerich protein
LS Schnabelrauch (1997)
Plant cell fractionation.
C. Price (1974)
10.1105/tpc.5.7.809
Isodityrosine cross-linking mediates insolubilization of cell walls in Chlamydomonas.
S. Waffenschmidt (1993)
10.1007/BF00043476
Production and harvesting of ionically wall-bound extensin from living cell suspension cultures
J. Miller (2004)
10.1105/tpc.6.12.1703
Function of Oxidative Cross-Linking of Cell Wall Structural Proteins in Plant Disease Resistance.
L. Brisson (1994)
10.1016/0885-5765(88)90039-2
Extracellular localization of chitinase in cucumber
T. Boller (1988)
10.1111/J.1365-313X.1993.TB00007.X
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth.
N. Carpita (1993)
10.1046/J.1365-313X.1997.12010097.X
Structural cell-wall proteins in protoxylem development: evidence for a repair process mediated by a glycine-rich protein.
U. Ryser (1997)
10.1271/BBB.59.2231
Purification and Characterization of a Glycine-rich Protein from the Aleurone Layer of Soybean Seeds
Miyoko Matsui (1995)
Structure and function of plant cellwall proteins
C Tiré (1993)
10.1016/S1360-1385(96)90005-9
Logjam at the Styx: Programmed cell death in plants
A. M. Jones (1996)
10.1093/OXFORDJOURNALS.AOB.A085501
On Vessel Member Differentiation in the Bean (Phaseolus vulgaris L.)
K. Esau (1978)
10.1002/j.1460-2075.1988.tb03243.x
Glycine‐rich cell wall proteins in bean: gene structure and association of the protein with the vascular system.
B. Keller (1988)
10.1104/pp.101.4.1127
Structural Cell Wall Proteins
B. Keller (1993)
10.1046/J.1365-313X.1994.05020157.X
Extensin: repetitive motifs, functional sites, post-translational codes, and phylogeny.
M. Kieliszewski (1994)
10.1086/297166
Redifferentiation of Single Mesophyll Cells into Tracheary Elements
H. Fukuda (1994)
10.1046/J.1365-313X.1999.00631.X
Identification of a novel peptide motif that mediates cross-linking of proteins to cell walls.
C. Domingo (1999)
10.1021/AR00021A002
SPIDER SILK : THE UNRAVELING OF A MYSTERY
R. Lewis (1992)
10.1105/tpc.3.10.1051
Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated.
B. Keller (1991)
10.1016/S1360-1385(97)84620-1
Tracheary element formation: building up to a dead end
M. McCann (1997)
10.1016/S0031-9422(00)80433-1
An intramolecular linkage involving isodityrosine in extensin
L. Epstein (1984)
10.1111/J.1432-1033.1991.TB16042.X
Metal affinity precipitation of proteins carrying genetically attached polyhistidine affinity tails.
G. Lilius (1991)
10.1016/0076-6879(74)31055-5
[52] Plant cell fractionation
C. Price (1974)
10.1146/ANNUREV.ARPLANT.49.1.281
PLANT CELL WALL PROTEINS.
G. Cassab (1998)
10.1105/tpc.5.1.9
Structure and function of plant cell wall proteins.
A. Showalter (1993)
10.1073/PNAS.86.3.896
Isolation of a complementary DNA encoding a chitinase with structural homology to a bifunctional lysozyme/chitinase.
J. P. Métraux (1989)
10.1073/PNAS.86.5.1529
Specific localization of a plant cell wall glycine-rich protein in protoxylem cells of the vascular system.
B. Keller (1989)



This paper is referenced by
10.3390/molecules15118641
Supramolecular self-assembled chaos: polyphenolic lignin's barrier to cost-effective lignocellulosic biofuels.
K. Achyuthan (2010)
10.1007/s00425-002-0938-7
Protoxylem: the deposition of a network containing glycine-rich cell wall proteins starts in the cell corners in close association with the pectins of the middle lamella
U. Ryser (2002)
10.1007/s00425-009-1089-x
A glycine-rich protein that facilitates exine formation during tomato pollen development
Kenneth J. McNeil (2009)
10.1111/j.1365-313X.2010.04270.x
The hydroxyproline-rich glycoprotein domain of the Arabidopsis LRX1 requires Tyr for function but not for insolubilization in the cell wall.
C. Ringli (2010)
10.1007/PL00000786
Glycine-rich proteins as structural components of plant cell walls
C. Ringli (2001)
10.1007/s10265-020-01223-x
Cell wall Glycine-rich Protein2 is involved in tapetal differentiation and pollen maturation.
Naomi Takebe (2020)
10.1360/02yc0172
Structure, expression pattern and chromosomal localization of the rice Osgrp-2 gene
Z. Liu (2008)
10.1007/s11105-019-01158-1
Identification of Candidate Genes Related to Stem Development in Brassica napus Using RNA-Seq
Rong Yuan (2019)
10.1111/J.1365-313X.2004.02208.X
Novel cell wall architecture of isoxaben-habituated Arabidopsis suspension-cultured cells: global transcript profiling and cellular analysis.
I. Manfield (2004)
10.1023/B:GROW.0000007507.98276.1d
Xylem-specific expression of a GRP1.8 promoter::4CL gene construct in transgenic tobacco
Hai Lu (2004)
10.1016/J.PLANTSCI.2004.03.017
A glycine-rich protein gene family predominantly expressed in tomato roots, but not in leaves and ripe fruit
Wan-Chi Lin (2005)
10.1007/s10265-006-0261-7
Identification and characterization of Arabidopsis thaliana genes involved in xylem secondary cell walls
R. Yokoyama (2006)
10.1016/S0168-9452(01)00585-4
Identification of novel genes differentially expressed in compatible and incompatible interactions between rice and Pseudomonas avenae
F. Che (2002)
10.1111/tpj.14479
Craterostigma plantagineum cell wall composition is remodelled during desiccation and the glycine-rich protein CpGRP1 interacts with pectins through clustered arginines.
Niklas Udo Jung (2019)
10.3929/ethz-a-004218037
LRX1, a chimeric LRR/extensin protein required for root hair morphogenesis in Arabidopsis thaliana, defines a new family of cell wall proteins
N. Baumberger (2001)
10.1371/journal.pone.0109735
Biochemical Aspects of the Soybean Response to Herbivory Injury by the Brown Stink Bug Euschistus heros (Hemiptera: Pentatomidae)
R. V. Timbó (2014)
10.1016/S1671-2927(09)60254-6
Characterization and Expression Analysis of Four Glycine-Rich RNA-Binding Proteins Involved in Osmotic Response in Tobacco (Nicotiana tabacum cv. Xanthi)
X. Chen (2010)
10.1590/S1415-47572007000500002
In silico analysis of phytohormone metabolism and communication pathways in citrus transcriptome
V. Quecini (2007)
10.1242/jcs.00966
A new structural element containing glycine-rich proteins and rhamnogalacturonan I in the protoxylem of seed plants
U. Ryser (2004)
10.3389/fpls.2017.00142
The Loricrin-Like Protein (LLP) of Phytophthora infestans Is Required for Oospore Formation and Plant Infection
T. Guo (2017)
Identification and characterization of a matrix metalloproteinase (Pta1-MMP) expressed during Loblolly pine (Pinus taeda) seed development and germination
Supriya M. Ratnaparkhe (2009)
10.1002/9781119312994.apr0070
Non-enzymic cell wall (glyco)proteins
K. Johnson (2003)
10.3390/proteomes2020224
Plant Cell Wall Proteins: A Large Body of Data, but What about Runaways?
Cécile Albenne (2014)
Genetic manipulation of auxin and ethylene production to alter the growth and development of Populus
Joo Young Kim (2007)
10.4161/psb.5.2.10336
Functional diversity of the plant glycine-rich proteins superfamily
Amanda Mangeon (2010)
10.1007/s00299-006-0128-3
Characterization of a novel glycine-rich protein from the cell wall of maize silk tissues
T. Tao (2006)
10.1186/1471-2229-12-207
ST proteins, a new family of plant tandem repeat proteins with a DUF2775 domain mainly found in Fabaceae and Asteraceae
L. Albornos (2012)
Semantic Scholar Logo Some data provided by SemanticScholar