Online citations, reference lists, and bibliographies.
← Back to Search

Interaction Of Cadmium With Glutathione And Photosynthesis In Developing Leaves And Chloroplasts Of Phragmites Australis (Cav.) Trin. Ex Steudel1

F. Pietrini, M. Iannelli, S. Pasqualini, A. Massacci
Published 2003 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
We investigated how the presence of cadmium (Cd) at the emergence of Phragmites australis Trin. (Cav.) ex Steudel plants from rhizomes interacted with leaf and chloroplast physiological and biochemical processes. About 8.5 nmol Cd mg–1 chlorophyll was found in leaves, and 0.83 nmol Cd mg–1 chlorophyll was found in chloroplasts of plants treated with 50 μm Cd. As a result, a 30% loss of chlorophyll was measured concomitantly with a comparable percentage reduction in light-saturated photosynthesis. Rubisco content and activity were lowered by 10% and 60%, respectively. Antioxidant activity was stimulated by Cd treatment and was associated with an increase in the glutathione and pyridine pools, and with a larger pool of reduced glutathione. It is suggested that the glutathione pool and its predominance in the reduced state protected the activity of many key photosynthetic enzymes against the thiophilic binding of Cd. Chloroplast ultrastructure was not significantly altered with 50 μm treatment and the efficiency of photosystem II, measured as the fluorescence ratio Fv/Fm, remained high because F0 and Fm were proportionally decreased. In plants treated with 100 μm Cd, all effects were exacerbated, but Fv/Fm remained close to that of control leaves and the glutathione and pyridine nucleotides pools were lowered. The results suggest that glutathione exerted a direct important protective role on photosynthesis in the presence of Cd.
This paper references
Determination of glutathione disulphide using glutathione reductase and 2vinylpyridine
RS Hutchison (1980)
Determination of glutathione disulphide using glutathione reductase and 2 - vinylpyridine
OW Griffith (1980)
Ascorbate peroxidasehydrogen peroxide scavenging enzyme in plants
N Atal (1992)
10.1093/JEXBOT/49.321.649
Review article. Glutathione homeostasis in plants: implications for environmental sensing and plant development
M. May (1998)
Glutathione transferase (human placenta).
B. Mannervik (1981)
10.1104/PP.126.3.993
Ozone quenching properties of isoprene and its antioxidant role in leaves.
F. Loreto (2001)
Oxidation reduction states of pyridine nucleotides measured by an adapted enzymatic cycling method in maize zea mays leaves submitted to anoxia
J. M. Carrier (1979)
10.1016/S0076-6879(80)69045-4
[43] Cycling assay for nicotinamide adenine dinucleotides
H. Matsumura (1980)
Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).
J. McCord (1969)
10.1104/PP.120.3.637
Sulfate transport and assimilation in plants
Leustek (1999)
10.1016/0076-6879(87)48036-1
CHLOROPHYLL AND CAROTENOIDS: PIGMENTS OF PHOTOSYNTHETIC BIOMEMBRANES
H. Lichtenthaler (1987)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding
JM Carrier (1976)
10.1016/S0269-7491(97)00110-3
Studies on cadmium toxicity in plants: a review.
P. Das (1997)
10.1093/JXB/49.321.649
Glutathione homeostasis in plants: implications for environmental sensing and plant development
M. J. May (1998)
10.1016/S0076-6879(81)77030-7
[28] Glutathione transferase (human placenta)
B. Mannervik (1981)
10.1016/S0098-8472(01)00106-X
A comparison of physiological indicators of sublethal cadmium stress in wetland plants
I. Mendelssohn (2001)
10.1146/ANNUREV.ARPLANT.51.1.371
PLANT THIOREDOXIN SYSTEMS REVISITED.
P. Schurmann (2000)
10.1007/BF00027175
Photometric method for routine determination of kcat and carbamylation of rubisco
T. Sharkey (2004)
10.1016/0003-2697(80)90139-6
Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine.
O. Griffith (1980)
10.1093/JXB/47.2.259
Environmental relevance of heavy metal-substituted chlorophylls using the example of water plants
H. Küpper (1996)
10.1093/OXFORDJOURNALS.PCP.A078181
Inhibition of the Chloroplast Photochemical Reactions by Treatment of Wheat Seedlings with Low Concentrations of Cadmium: Analysis of Electron Transport Activities and Changes in Fluorescence Yield
N. Atal (1991)
10.1007/978-94-009-0511-5
Current Research in Photosynthesis
M. Baltscheffsky (1990)
10.1023/A:1022458108678
Rubisco activase – Rubisco's catalytic chaperone
A. Portis (2004)
10.1093/JXB/49.321.623
Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants
G. Noctor (1998)
10.1016/S0981-9428(02)01455-9
Antioxidant response to cadmium in Phragmites australis plants
M. Iannelli (2002)
10.1111/J.1365-3040.1994.TB00144.X
Does Rubisco control the rate of photosynthesis and plant growth? An exercise in molecular ecophysiology
M. Stitt (1994)
Heavy metal tolerance genes : prospective tools for phytoremediation
AR Portis (1996)
10.1007/s004250000439
Leaf chlorosis in oilseed rape plants (Brassica napus) grown on cadmium-polluted soil: causes and consequences for photosynthesis and growth
A. Baryla (2001)
Carbon metabolism in leaves of cadmium treated wheat seedlings
Darshan Malik (1992)
ASSAY OF CATALASES AND PEROXIDASES, IN METHODS IN ENZYMOLOGY
B. Chance (1995)
10.1023/A:1006132608181
In situ detection of heavy metal substituted chlorophylls in water plants
H. Küpper (2004)
10.1104/pp.002659
Cadmium-Induced Sulfate Uptake in Maize Roots1
F. Nocito (2002)
Relationship between the quantum yield of photosynthetic electron transport and the quenching of chlorophyll fluorescence
OW Griffith (1989)
10.1046/J.1469-8137.2000.00560.X
Cadmium accumulation in populations of Thlaspi caerulescens and Thlaspi goesingense
E. Lombi (2000)
Phytoxicity of cadmium ions on germinating seedlings of mung bean ( Phaseolus vulgaris ) : involvement of lipid peroxides in chlorophyll degradation
M Stitt (1992)
10.1046/j.1529-8817.2002.01148.x
HEAVY METAL‐INDUCED INHIBITION OF PHOTOSYNTHESIS: TARGETS OF IN VIVO HEAVY METAL CHLOROPHYLL FORMATION1
H. Küpper (2002)
10.1006/ABBI.1996.0344
Protein S-thiolation and regulation of microsomal glutathione transferase activity by the glutathione redox couple.
A. L. Dafré (1996)
Phytoremediation of metals . Tolerance mechanisms against oxidative stress
F Navari-Izzo (2001)
10.1038/227680A0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
10.1046/J.1365-3040.2002.00850.X
Cadmium causes the oxidative modification of proteins in pea plants
M. C. Romero-Puertas (2002)
10.1021/BI0001978
Differential effects of chilling-induced photooxidation on the redox regulation of photosynthetic enzymes.
R. S. Hutchison (2000)
10.1104/PP.010318
Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots.
A. Schützendübel (2001)
Regulation of glutathione synthesis and its role in abiotic and biotic stress
LJ De Kok (2000)
10.1006/ABIO.1997.2283
Cycling assay for nicotinamide adenine dinucleotides: NaCl precipitation and ethanol solubilization of the reduced tetrazolium.
Y. Gibon (1997)
Feedback limitation of photosynthesis and the physiological role of ribulose bisphosphate carboxylase carbamylation
T. Sharkey (1990)
10.1016/S0168-9452(98)00159-9
The stage of leaf maturity implicates the response of the photosynthetic apparatus to cadmium toxicity
Z. Krupa (1998)
Sulfur Nutrition and Sulfur Assimilation in Higher Plants: Molecular, Biochemical and Physiological Aspects
C. Brunold (1990)
Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts.
K. Cline (1985)
10.1046/J.1365-313X.1996.10061017.X
Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings: changes in antioxidant system, oxidation of proteins and lipids, and protease activities
T. K. Prasad (1996)
10.1111/J.1399-3054.1992.TB04728.X
Ascorbate peroxidase – a hydrogen peroxide‐scavenging enzyme in plants
K. Asada (1992)
10.1093/OXFORDJOURNALS.PCP.A077996
Scavenging of Hydrogen Peroxide in the Endosperm of Ricinus communis by Ascorbate Peroxidase
S. Klapheck (1990)
10.1016/S0304-4165(89)80016-9
The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence
B. Genty (1989)
Antioxidant response to cadmium in Phragmites australis plants
S Klapheck (2002)
10.1111/J.1399-3054.1992.TB05267.X
Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorphyll degradation
B. V. Somashekaraiah (1992)
10.1016/S0168-9452(02)00017-1
Cadmium uptake and subcellular distribution in plants of Lactuca sp. Cd-Mn interaction
I. Ramos (2002)
10.1007/BF00384257
Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves
S. V. Caemmerer (2004)
Regulation of glutathione synthesis and its role in abiotic and biotic stress
H Rennenberg (2000)
10.1016/S0168-9452(96)04483-4
Mechanisms of salt tolerance in a cell line of Pisum sativum: biochemical and physiological aspects
E. Olmos (1996)
10.1078/0176-1617-00639
Physiological and biochemical aspects of cadmium toxicity and protective mechanisms induced in Phragmites australis and Typha latifolia
Erika Fediuc (2002)
10.1093/JXB/50.338.1533
Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.)
Y. Fracheboud (1999)
10.1016/0003-2697(88)90564-7
Assay of glutathione reductase in crude tissue homogenates using 5,5'-dithiobis(2-nitrobenzoic acid).
I. K. Smith (1988)
10.1093/JEXBOT/49.321.623
Review article. Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants
G. Noctor (1998)
10.1016/S0921-3449(96)01174-3
Heavy metal tolerance genes: prospective tools for bioremediation
D. Ow (1996)
Mechanisms of chillinginduced oxidative stress injury and tolerance in developing maize seedlings : changes in antioxidant system , oxidation of proteins and lipids , and proteases activities
I Ramos (1996)
10.1007/s004250000458
Molecular mechanisms of plant metal tolerance and homeostasis
S. Clemens (2001)
10.1016/S0098-8472(98)00058-6
Response to cadmium in higher plants
L. S. D. Toppi (1999)
10.1023/A:1007097518297
Cd/Fe Interaction in Higher Plants - Its Consequences for the Photosynthetic Apparatus
A. Siedlecka (2004)
Heavy metal tolerance genes : prospective tools for phytoremediation
AR Portis (1996)
10.1002/9780470110171.CH14
The assay of catalases and peroxidases.
A. Maehly (1954)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)



This paper is referenced by
10.1007/s11120-013-9861-y
Variation in Rubisco content and activity under variable climatic factors
J. Galmés (2013)
10.1007/s10725-014-9973-1
Differences in photosynthesis, yield and grain cadmium accumulation as affected by exogenous cadmium and glutathione in the two rice genotypes
Fangbin Cao (2014)
10.1016/B978-0-12-813066-7.00010-3
Targeting the Redox Regulatory Mechanisms for Abiotic Stress Tolerance in Crops
Punam Kundu (2018)
10.1007/978-90-481-9370-7
Plant Adaptation and Phytoremediation
M. Ashraf (2010)
10.1016/j.plaphy.2008.06.012
Ultrastructural changes, zinc hyperaccumulation and its relation with antioxidants in two ecotypes of Sedum alfredii Hance.
Xiao Fen Jin (2008)
10.1016/j.jhazmat.2011.09.085
Lead tolerance and cellular distribution in Elsholtzia splendens using synchrotron radiation micro-X-ray fluorescence.
Jie Zhang (2011)
10.1007/978-94-007-5179-8_13
Cadmium toxicity in plants.
E. Andresen (2013)
10.1007/s10661-013-3295-z
Small effects of a large sediment contamination with heavy metals on aquatic organisms in the vicinity of an abandoned lead and zinc mine
D. Ciszewski (2013)
10.1016/j.jplph.2013.06.009
Detoxification of cadmium (Cd) by a novel Cd-associated and Cd-induced molecule in the stem of common reed.
Kyoko Higuchi (2013)
10.1016/j.ecoenv.2014.06.027
Antioxidant response of Phragmites australis to Cu and Cd contamination.
A. C. Rocha (2014)
Potential use of pineapple (Ananas comosus L.) and cadmium tolerant bacteria to reduce cadmium toxicity in soil
Phatthanawan Promnim (2012)
10.1016/J.PLANTSCI.2005.05.030
Effects of cadmium hyperaccumulation on physiological characteristics of Sedum alfredii Hance (Crassulaceae)
W. Zhou (2005)
10.11403/JSET.11.1
Glutathione-Ascorbate Cycle for Phytoremediation of Mercury by Eichhornia crassipes (Mart.) Solms
Upma Narang (2008)
10.1186/1471-2229-11-75
Arbuscular mycorrhizal symbiosis elicits shoot proteome changes that are modified during cadmium stress alleviation in Medicago truncatula
Achref Aloui (2010)
10.1007/s10534-013-9608-4
Nitric oxide (NO) counteracts cadmium induced cytotoxic processes mediated by reactive oxygen species (ROS) in Brassica juncea: cross-talk between ROS, NO and antioxidant responses
K. Verma (2013)
10.17957/IJAB/15.0141
Morpho-physiological and Biochemical Responses of Camelina (Camelina sativa crantz) Genotypes under Drought Stress
Zeeshan Adib Ahmed (2017)
10.1007/s10457-017-0141-2
Phytoremediation of cadmium-contaminated soil through multipurpose tree species
B. Kaur (2017)
10.1590/1519-6984.171961
Cadmium tolerance of Typha domingensis Pers. (Typhaceae) as related to growth and leaf morphophysiology.
J. V. Oliveira (2018)
10.1007/s10535-008-0140-2
Antioxidative response to cadmium in roots and leaves of tomato plants
W. B. Ammar (2008)
10.1007/s10646-010-0543-7
Perspectives for genetic engineering of poplars for enhanced phytoremediation abilities
R. Yadav (2010)
10.1155/2012/872875
Molecular Mechanism of Heavy Metal Toxicity and Tolerance in Plants: Central Role of Glutathione in Detoxification of Reactive Oxygen Species and Methylglyoxal and in Heavy Metal Chelation
M. Hossain (2012)
10.1007/s11356-015-5959-4
Alleviation of lead-induced physiological, metabolic, and ultramorphological changes in leaves of upland cotton through glutathione
M. Khan (2016)
10.1007/s00128-017-2143-1
The Difference of Photosynthetic Responses to the Cadmium Stress Between a Wild Soybean (Glycine soja Sieb. et Zucc.) and a Cultivated Soybean
Zhongcai Xue (2017)
10.4236/AS.2016.82010
Ameliorative Effect of Chelating Agents on Photosynthetic Attributes of Cd Stressed Sunflower
Rumana Sadiq (2017)
10.1556/AAGR.57.2009.3.7
Role of salicylic acid in regulation of cadmium toxicity in wheat ( Triticum aestivum L.)
H. Moussa (2009)
10.1111/pce.12234
Differential cadmium and zinc distribution in relation to their physiological impact in the leaves of the accumulating Zygophyllum fabago L.
Isabelle S. Lefèvre (2014)
10.5352/JLS.2010.20.11.1667
Influence of Nitrate on Growth, Chlorophyll Content, Content and Activity of Rubisco and Rubisco Activase of Tobacco Plant Treated with Cadmium in vitro
Kwang Soo Roh (2010)
Evaluation of Australian Native Wetland Plants for Phytoremediation of Saline Mine-Leachate
Allan Adams (2014)
Impact of Soil Cadmium Contamination on Accumulation of Cadmium and Proline Content of Pinus sylvestris L. Seedling
Sakineh Modirroosta (2014)
10.1016/B978-0-12-803158-2.00011-4
Glutathione and Phytochelatins Mediated Redox Homeostasis and Stress Signal Transduction in Plants: An Integrated Overview
Shweta Singh (2016)
10.1007/s10750-018-3629-5
Strategies for lead distribution in organs of Phragmites australis (Cav.) Trin. ex Steud. (Common reed) subjected to Pb pollution in flood and drought environments
N. Zhang (2018)
10.1007/S10863-007-9098-6
Enhanced alternative oxidase and antioxidant enzymes under Cd2+ stress in Euglena
Norma A. Castro-Guerrero (2008)
See more
Semantic Scholar Logo Some data provided by SemanticScholar