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Glutathione Causes A Massive And Selective Induction Of Plant Defense Genes.
V. Wingate, M. A. Lawton, C. Lamb
Published 1988 · Biology, Medicine
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The reduced form of glutathione (GSH), when supplied to suspension cultured cells of bean (Phaseolus vulgaris L.) at concentrations in the range 0.01 to 1.0 millimolar, stimulates transcription of defense genes including those that encode cell wall hydroxyproline-rich glycoproteins and the phenylpropanoid biosynthetic enzymes phenylalanine ammonialyase (PAL) and chalcone synthase (CHS) involved in lignin (PAL) and phytoalexin (PAL, CHS) production. Transcriptional activation of these genes leads to marked accumulation of the corresponding transcripts, contributing to a massive change in the overall pattern of protein synthesis which closely resembles that previously observed in response to fungal elicitor. GSH causes a marked increase in extractable PAL activity, whereas the oxidized form of glutathione, constituent amino acids, or other reducing agents are inactive. Possible roles of GSH in signaling biological stress are discussed.
This paper references
Effect of heat shock on the metabolism of glutathione in maize roots.
J. Nieto-Sotelo (1986)
Reduced and oxidised glutathione and glutathione reductase in tissues of Pisum sativum
W BIELAWSKI (1986)
Elicitor induction of mRNA activity. Rapid effects of elicitor on phenylalanine ammonia-lyase and chalcone synthase mRNA activities in bean cells.
M. A. Lawton (1983)
Elicitor rapidly induces chalcone synthase mRNA in Phaseolus vulgaris cells at the onset of the phytoalexin defense response.
T. Ryder (1984)
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
P. Thomas (1980)
Effects of biotic and abiotic elicitors on phytoalexin metabolism in soybean
P. Moesta (1980)
The regulation of the biosynthesis of glutathione in leaves of barley (Hordeum vulgare L.)
I. K. Smith (1985)
Transcriptional activation of plant defense genes by fungal elicitor, wounding, and infection.
M. A. Lawton (1987)
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
Gary Kent Mcmaster (1977)
Glutathione metabolism and possible biological roles in higher plants
H. Rennenberg (1980)
Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines.
J. I. Garrels (1979)
A New Thiol in Legumes
C. Price (1957)
EFFECTS OF FROST-HARDENING AND SALINITY ON GLUTATHIONE AND SULFHYDRYL LEVELS AND ON GLUTATHIONE-REDUCTASE ACTIVITY IN SPINACH LEAVES
L. D. Kok (1983)
Rapid transient induction of phenylalanine ammonia-lyase mRNA in elicitor-treated bean cells.
K. Edwards (1985)
Sulfate transport in cultured tobacco cells.
I. K. Smith (1975)
Intramolecular disulfide bridges: avenues to receptor activation?
C. Malbon (1987)
Superoxide anion generation: a response of potato leaves to infection with Phytophthora infestans
Hee Byung Chai (1987)
Induction of Medicarpin Biosynthesis in Ladino Clover Callus by p-Chloromercuribenzoic Acid Is Reversed by Dithiothreitol.
D. L. Gustine (1987)
Effect of heat shock on the metabolism of glutathione in maize
J NIETO-SOTELO (1986)
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
W. Bonner (1974)
Regulation by sulfhydryl groups of glyceollin accumulation in soybean hypocotyls
P. Stössel (2004)
Phytochelatins: The Principal Heavy-Metal Complexing Peptides of Higher Plants
E. Grill (1985)
Comparison of glutathione S-transferases of Zea mays responsible for herbicide detoxification in plants and suspension-cultured cells
R. Edwards (2004)
Formation and activity of phaseollin in the interaction between bean hypocotyls (Phaseolus vulgaris) and physiological races of Colletotrichum lindemuthianum
J. A. Bailey (1971)
Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants.
D. Corbin (1987)
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R. Scheibe (2005)
Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis–banana interaction
O. Portal (2011)
Molecular Biology of Plant Cell Wall Hydroxyproline-Rich Glycoproteins
A. Showalter (1990)
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Alteration of gene expression in Pisum sativum tissue cultures caused by the free radical‐generating agent 2,2′‐azobis (2‐amidinopropane) dihydrochloride
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L. Loyall (2000)
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C. Bowler (2000)
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Jiahong Shao (2004)
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Bao-yu Hu (2018)
Glutathione status and glutathione reductase activity in spruce needles of healthy and damaged trees at two mountain sites.
U. Schmieden (1993)
N-Acetylcysteine-dependent protection against UV-B damage in two photosynthetic organisms
G. Malanga (1999)
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Genotypic variation in phytoremediation potential of Brassica juncea cultivars exposed to Cd stress
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