← Back to Search
Ultrastructural Localization Of A Bean Glycine-Rich Protein In Unlignified Primary Walls Of Protoxylem Cells.
U. Ryser, B. Keller
Published 1992 · Biology, Medicine
Download PDFAnalyze on Scholarcy
A polyclonal antibody was used to localize a glycine-rich cell wall protein (GRP 1.8) in French bean hypocotyls with the indirect immunogold method. GRP 1.8 could be localized mainly in the unlignified primary cell walls of the oldest protoxylem elements and also in cell corners of both proto- and metaxylem elements. In addition, GRP 1.8 was detected in phloem using tissue printing. The labeled primary walls of dead protoxylem cells showed a characteristically dispersed ultrastructure, resulting from the action of hydrolases during the final steps of cell maturation and from mechanical stress due to hypocotyl growth. Primary walls of living protoxylem and adjacent parenchyma cells were only weakly labeled. This was true also for the secondary walls of proto- and metaxylem cells, which in addition showed high background labeling. Inhibition of lignification with a specific and potent inhibitor of phenylalanine ammonia-lyase did not lead to enhanced labeling of secondary walls, showing that lignin does not mask the presence of GRP 1.8 in these walls. Dictyosomes of living proto- and metaxylem cells were not labeled, but dictyosomes of xylem parenchyma cells without secondary walls, adjacent to strongly labeled protoxylem elements, were clearly labeled. These observations suggest that GRP 1.8 is not produced by xylem vessels but by xylem parenchyma cells that export the protein to the wall of protoxylem vessels.
This paper references
Cell Wall Proteins
A. Cassab (1988)
Antibody localization of extensin in cell walls of carrot storage roots
J. P. Stafstrom (2004)
Characterization of two soybean repetitive proline-rich proteins and a cognate cDNA from germinated axes.
K. Datta (1989)
Tissue-Specific Expression of Cell Wall Proteins in Developing Soybean Tissues.
Z. Ye (1991)
lnhibitors of phenylalanine ammonia - lyase : 2 - Aminoindan - 2 - phosphonic acid and related compounds
J. Zon (1992)
Developmentally regulated expression of soybean proline-rich cell wall protein genes.
J. C. Hong (1989)
Specific expression of a novel cell wall hydroxyproline-rich glycoprotein gene in lateral root initiation.
B. Keller (1989)
A Cytochemical Investigation of the Lateral Walls of Dianthus Vessels. Differentiation and Pit-Membrane Formation
A. M. Catesson (1983)
Plant Anatomy, 2nd ed
K. Esau (1965)
Specific localization of a plant cell wall glycine-rich protein in protoxylem cells of the vascular system.
B. Keller (1989)
lnhibitors of phenylalanine ammonialyase: 2-Aminoindan-2-phosphonic
J. Zon (1992)
A. Allsopp (1966)
On Vessel Member Differentiation in the Bean (Phaseolus vulgaris L.)
K. Esau (1978)
The glycine-rich cell wall proteins of higher plants.
C. M. Condit (1990)
Glycine‐rich cell wall proteins in bean: gene structure and association of the protein with the vascular system.
B. Keller (1988)
Stereological Methods. Practical methods for biological morphometry
E Weibel (1979)
Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper
G. Cassab (1987)
Deposition of glycine-rich structural protein in xylem cell walls of French bean seedlings is independent of lignification
B. Keller (1990)
Primary vascular tissues
T. P. OBrien (1974)
E R Weibel (1979)
Xylem Cell Development
J. Barnett (1981)
A gene encoding a novel glycine-rich protein of petunia
C. M. Condit (1986)
Stereological methods. Vol. 1: Practical methods for biological morphometry By . Academic Press, New York/London, 1979. xvi + 415 pp., $57.50
G. Hung (1984)
Comparative localization of three classes of cell wall proteins.
Z. Ye (1991)
lmmunocytolocalization of extensin in developing soybean seedcoat by immunogold-silver
A novel glycine-rich cell wall protein in rice
M. Lei (1991)
Differential expression of five Arabidopsis genes encoding glycine-rich proteins.
D. E. de Oliveira (1990)
Developmentally regulated expression of soybean proline-rich
J. C. Hong (1989)
Characterization of the expression of the petunia glycine-rich protein-1 gene product.
C. M. Condit (1990)
This paper is referenced by
A proline-, threonine-, and glycine-rich protein down-regulated by drought is localized in the cell wall of xylem elements.
H. Harrak (1999)
New microsome-associated HT-family proteins from Nicotiana respond to pollination and define an HT/NOD-24 protein family.
K. Kondo (2008)
PLANT CELL WALL PROTEINS.
G. Cassab (1998)
Isolation and characterization of a cDNA from Quercus robur differentially expressed in juvenile-like and mature shoots.
B. Gil (2003)
Vascular Cell Differentiation
H. Kuriyama (2007)
J E Varner (1994)
Common occurrence of homologues of petunia glycine-rich protein-1 among plants
Shu-Hua Cheng (2004)
Root adaptive responses of tall fescue (Festuca arundinacea) growing in sand treated with petroleum hydrocarbon contamination
A. Balasubramaniyam (2012)
Mitodepressive and clastogenic effects of some aminophosphonates, inhibitors of phenylalanine ammonia-lyase. I. 2-aminoindan-2-phosphonic acid
R. Osiecka (1998)
The Cell Wall: A Sensory Panel for Signal Transduction
Keiko Sugimoto-Shirasu (2018)
Root cap-specific genes in Zea mays
T. Matsuyama (1999)
Genome-wide identification and phylogenetic analysis of plant RNA binding proteins comprising both RNA recognition motifs and contiguous glycine residues
M. Lewinski (2015)
Structural Cell Wall Proteins
B. Keller (1993)
Tissue- and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants
C. Feuillet (2004)
Monoclonal antibodies to cell-specific cell surface carbohydrates in plant cell biology and development.
R. Pennell (1995)
Specific interaction of the tomato bZIP transcription factor VSF-1 with a non-palindromic DNA sequence that controls vascular gene expression
C. Ringli (2004)
Root-specific expression of a Zea mays gene encoding a novel glycine-rich protein, zmGRP3
M. Goddemeier (2004)
The nodule-specific VfENOD-GRP3 gene encoding a glycine-rich early nodulin is located on chromosome I of Vicia faba L. and is predominantly expressed in the interzone II-III of root nodules
H. Küster (2004)
Identification and characterization of Arabidopsis thaliana genes involved in xylem secondary cell walls
R. Yokoyama (2006)
Plant Cell Walls
N. Carpita (2001)
Structure and Expression of Genes Coding for Structural Proteins of the Plant Cell
Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation
D. Xu (2004)
Survey of glycine-rich proteins (GRPs) in the Eucalyptus expressed sequence tag database (ForEST)
S. Bocca (2005)
Methyl recycling activities are co-ordinately regulated during plant development.
L. A. Pereira (2007)
The use of versatile plant antimicrobial peptides in agribusiness and human health
E. S. Cândido (2014)
Functional diversity of the plant glycine-rich proteins superfamily
Amanda Mangeon (2010)
Differential expression of genes encoding cell wall proteins in vascular tissues from vertical and bent loblolly pine trees.
Y. Zhang (2000)
The Last Step of Syringyl Monolignol Biosynthesis in Angiosperms Is Regulated by a Novel Gene Encoding Sinapyl Alcohol Dehydrogenase
L. Li (2001)
Ovule Development : ldentification of Stage-Specific and Tissue-Specif ic cDNAs
J. Nadeau (2002)
LRX1, a chimeric LRR/extensin protein required for root hair morphogenesis in Arabidopsis thaliana, defines a new family of cell wall proteins
N. Baumberger (2001)
Hydrophobic interactions of the structural protein GRP1.8 in the cell wall of protoxylem elements.
C. Ringli (2001)
A novel extracellular matrix protein from tomato associated with lignified secondary cell walls.
C. Domingo (1994)See more