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Programmed Cell Death In Terminally Differentiating Keratinocytes: Role Of Endogenous Endonuclease.
C. McCall, J. J. Cohen
Published 1991 · Biology, Medicine
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Mammalian epithelium is a tissue with a very high turnover rate. It consists of a rapidly proliferating compartment comprising basal and suprabasal keratinocytes, from which cells move upwards while differentiating into granular keratinocytes. The end product is shed as an enucleate corneocyte, which has a mechanically rigid, chemically resistant cross-linked keratinous envelope. The loss of the nucleus occurs specifically in the granular keratinocyte layer; here, cells with the classical apoptotic morphology of clumped and marginated condensed chromatin may be observed. This morphology is characteristic of "programmed" cell death in other systems, of which the lymphocyte has been most extensively studied, and is associated with the cleavage of nuclear DNA into nucleosome-sized fragments. In the present investigation we separated newborn mouse skin into basal and granular keratinocyte fractions and examined the state of the DNA in each fraction. Our results indicate that cells in the basal layer, while their DNA is perfectly intact, are preparing to die. DNA fragmentation is initiated in the granular keratinocyte layer and is identical in pattern to that seen in other examples of programmed cell death.
This paper references
Gene induction by gamma-irradiation leads to DNA fragmentation in lymphocytes.
K. Sellins (1987)
Internal Disintegration Model of Cytotoxic Lymphocyte‐Induced Target Damage
J. H. Russell (1983)
IL-2 addiction: withdrawal of growth factor activates a suicide program in dependent T cells.
R. C. Duke (1986)
Terminal differentiation of cultured human epidermal cells
H. Green (1977)
Cell death: a new classification separating apoptosis from necrosis
A. Wyllie (1981)
Separation of newborn rat epidermal cells on discontinuous isokinetic gradients of PERCOLL.
M. Brysk (1981)
Serum contains a platelet-derived transforming growth factor.
C. B. Childs (1982)
APOPTOSIS IN HUMAN EPIDERMIS: A POSTMORTEM STUDY BY LIGHT AND ELECTRON MICROSCOPY *
J. G. Lovas (1986)
Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
D. Hewish (1973)
Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death.
J. Cohen (1984)
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
DNA fragmentation in targets of CTL: an example of programmed cell death in the immune system.
J. J. Cohen (1985)
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
A. Wyllie (1980)
Type beta transforming growth factor is the primary differentiation-inducing serum factor for normal human bronchial epithelial cells.
T. Masui (1986)
Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.
R. C. Duke (1983)
Apoptosis: Its nature and implications for dermatopathology
D. Weedon (1979)
Cell death in biology and pathology
I. Bowen (1981)
Apoptotic bodies and abnormally dividing epithelial cells in squamous cell carcinoma
N. el-Labban (1986)
Identification of a glucocorticoid-induced nuclease in thymocytes. A potential "lysis gene" product.
M. M. Compton (1987)
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J. Winkler (1999)
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Basement membrane proteins promote progression of intraepithelial neoplasia in 3‐dimensional models of human stratified epithelium
F. Andriani (2004)
Human corneal epithelial cell viability and morphology after dilute alcohol exposure.
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A. Kuhn (2008)
Identification of genes involved in programmed cell death
G. Owens (2004)
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E. Coucouvanis (1995)
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T. Sato (1997)
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V. Chaturvedi (2006)
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B. Forslind (1999)
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I. Scordi (1999)
HPV16 E6 oncoprotein inhibits apoptosis induced during serum-calcium differentiation of foreskin human keratinocytes.
J. Alfandari (1999)
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G. Plewig (2003)
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C. C. Matthews (1997)
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