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Effect Of Thymus Vulgaris Essential Oil On Intestinal Bacterial Microbiota Of Rainbow Trout, Oncorhynchus Mykiss (Walbaum) And Bacterial Isolates

P. Navarrete, Isabela V. Toledo, P. Mardones, Rafael Opazo, R. Espejo, J. Romero
Published 2010 · Biology

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The application of natural and innocuous compounds has potential in aquaculture as an alternative to antibiotics. We evaluated the eiect of diet supplementation with Thymus vulgaris essential oil (TVEO) on the allochthonous microbial composition of rainbow trout. DNA was extracted directly from the intestinal contents, and the V3-V4 regions of the 16S rRNA genes were ampli¢ed by PCR. The bacterial composition was analysed using temporal temperature gradient electrophoresis (TTGE). No signi¢cant changes (P40.05) were detected in the TTGE pro¢les of TVEO-treated trout compared with the controls. The Dice similarity index revealed a high stability (Cs 470%) of the intestinal microbiota in both groups during the 5-week period. Sequence analyses of the TTGE bands revealed the same bacterial composition in both groups, with most bacteria belonging to the Proteobacteria and Firmicutes phyla. The in vitro antibacterial activity of TVEO was assessed using a range of normal intestinal isolates and ¢sh pathogens. The inhibitory concentrations for all the tested bacteria were higher than theTVEO levels used in trout, which may explain the in vivo results.
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