Online citations, reference lists, and bibliographies.
← Back to Search

Chlorophyll Breakdown In Senescent Leaves: Demonstration Of Mg‐dechelatase Activity

M. Langmeier, S. Ginsburg, P. Matile
Published 1993 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
The action of Mg-dechelatase was brought to light by incubating senescent rape cotyledons or chloroplasts under conditions which prevented the oxidative cleavage of chlorophyll-porphyrin. The accumulation of chlorophyllide and pheophorbide taking place under such conditions was considered as a measure of apparent activities of chlorophyllase and dechelatase, respectively. In excised cotyledons metal chelators such as 2,2′-dipyridyl and o-phenanthroline caused a marked accumulation of pheophorbide a, without affecting the apparent activity of chlorophyllase. Treatment of cotyledons with an inhibitor of cytoplasmic protein synthesis d-2-(4-methyl-2,6-dinitroanilino)-N-methyl-propionamide (d-MDMP) caused a reduced accumulation of pheophorbide a in the presence of dipyridyl, suggesting that the appearance and maintenance of Mg-dechelatase activity in senescent cotyledons requires continuous cytoplasmic protein synthesis. In isolated senescent chloroplasts (gerontoplasts) the cleavage of chlorophyll-porphyrin requires the supplementation with glucose-6-phosphate (Glc6P). Upon the incubation of gerontoplasts in the absence of Glc6P, a conspicuous accumulation of pheophorbide a occurred. Much smaller pools of pheophorbide a were produced when porphyrin cleavage was allowed in the presence of Glc6P. These phenomena were not observed in pre-senescent chloroplasts. In contrast to the apparent Mg-dechelatase activity, chlorophyllase activity did not change in a senescent-specific fashion. The lysis of gerontoplasts by freezing and thawing caused an enhancement of apparent chlorophyllase activity whereas the activity of Mg-dechelatase was lower than in the intact organelles. In the pre-senescent chloroplasts, lysis evoked a small apparent Mg-dechelatase activity, suggesting that in a latent form this enzyme may be present even before the onset of foliar senescence.
This paper references
10.1093/OXFORDJOURNALS.PCP.A076798
Temperature-Dependent Inhibitive Actions of α,α′-Dipyridyl and Cycloheximide on the Senescence of Maize Leaves
C. Pjon (1984)
10.1016/S0176-1617(11)81659-7
A Novel Type of Chlorophyll Catabolite in Senescent Barley Leaves
K. Bortlik (1990)
10.1016/S0044-328X(83)80181-0
Chlorophyll Degradation in Senescent Tobacco Cell Culture (Nicotiana tabacum var. «Samsun»)
S. Schoch (1983)
10.1016/0304-4211(76)90119-X
Delayed senescence in leaves treated with the protein synthesis inhibitor MDMP
H. Thomas (1976)
10.1007/BF02310413
Separation of chlorophylls and their degradation products in marine phytoplankton by reversed-phase high-performance liquid chromatography
M. Zapata (1987)
Breakdown of chlorophyll : constitution of a secoporphinoid chlorophyll catabolite isolated from senescent barley leaves
B. Kräutler (1992)
10.1104/PP.56.1.140
The Metabolism of Oat Leaves during Senescence: IV. The Effects of alphaalpha'-Dipyridyl and other Metal Chelators on Senescence.
R. Tetley (1975)
10.1093/OXFORDJOURNALS.PCP.A078056
ENZYMATIC DEGRADATION OF CHLOROPHYLL IN CHENOPODIUM ALBUM
Y. Shioi (1991)
10.1111/J.1469-8137.1989.TB04211.X
Catabolism of chlorophyll in vivo: significance of polar chlorophyll catabolites in a non-yellowing senescence mutant of Festuca pratensis Huds.
H. Thomas (1989)
10.1016/S0176-1617(11)80214-2
Breakdown of Chlorophyll in Chloroplasts of Senescent Barley Leaves Depends on ATP
M. Schellenberg (1990)
10.1016/S0015-3796(82)80057-7
Prevention by Kinetin of Ethylene-induced Chlorophyllase Activity in Senescing Detached Leaves of Helianthus annuus
S. S. Purohit (1982)
10.1016/S0176-1617(11)80211-7
Radiolabelling of Chlorophyll for Studies on Catabolism
Christian Peisker (1990)
10.1073/PNAS.84.7.1901
Chlorophyll catabolism in senescing plant tissues: In vivo breakdown intermediates suggest different degradative pathways for Citrus fruit and parsley leaves.
D. Amir-Shapira (1987)
10.1093/OXFORDJOURNALS.PCP.A076491
Effects of Metal Chelators on Leaf Senescence in Maize and Hydrangea
C. Pjon (1982)
10.1002/ANIE.199113151
On the enigma of chlorophyll degradation:the constitution of a secoporphinoid catabolite.
B. Kräutler (1991)
10.1093/OXFORDJOURNALS.PCP.A075344
A requirement for cytoplasmic protein synthesis during chloroplast senescence in the aquatic plant Anacharis canadensis
Sarah Makovetzki (1976)
10.1073/PNAS.85.24.9529
Catabolites of chlorophyll in senescing barley leaves are localized in the vacuoles of mesophyll cells.
P. Matile (1988)
10.1016/0031-9422(90)83014-R
Pyropheophorbide a, a catabolite of ethylene-induced chlorophyll a degradation.
K. Shimokawa (1990)
10.1111/J.1399-3054.1978.TB02577.X
Control of Chlorophyll Degradation in Detached Leaves of Barley and Oat through Effect of Kinetin on Chlorophyllase Levels
B. Sabater (1978)
10.1007/BF00390814
Chlorophyllase activity in developing leaves of Phaseolus vulgaris L.
W. Moll (2004)
10.1016/S0176-1617(88)80115-9
Enzymatic Formation of Pheophorbide and Pyropheophorbide during Chlorophyll Degradation in a Mutant of Chlorella fusca Shihira Ee Kraus
R. Ziegler (1988)
10.1104/pp.102.2.521
Identification of Catabolites of Chlorophyll-Porphyrin in Senescent Rape Cotyledons
S. Ginsburg (1993)
10.1016/S0176-1617(89)80099-9
Phytol and the Breakdown of Chlorophyll in Senescent Leaves
Christian Peisker (1989)
10.1016/S0176-1617(87)80094-9
Degradation of Chlorophyll and Chlorophyllase Activity in Senescing Barley Leaves
M. T. Rodríguez (1987)
10.1515/znc-1981-9-1022
Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of Euglena1
S. Schoch (1981)
10.1016/0076-6879(87)48036-1
CHLOROPHYLL AND CAROTENOIDS: PIGMENTS OF PHOTOSYNTHETIC BIOMEMBRANES
H. Lichtenthaler (1987)
10.1016/0014-5793(91)81168-8
Chlorophyll catabolism in Chlorella protothecoides Isolation and structure elucidation of a red bilin derivative
N. Engel (1991)



This paper is referenced by
10.1078/0176-1617-01210
Chlorophyll and carotenoid degradation mediated by thylakoid-associated peroxidative activity in olives (Olea europaea) cv. hojiblanca.
B. Gandul-Rojas (2004)
10.1007/s10457-017-0136-z
Morphological and physiological responses of Guazuma ulmifolia Lam. to different pruning dates
Eloísa Ortega-Vargas (2017)
10.17475/kastorman.498746
Effects of Iron-Steel Factory and Dense Traffic on Leaf Chemical Compounds of Six Tree Species in Turkey
Nezahat Turfan (2018)
10.1039/b9pp00018f
Demetalation kinetics of natural chlorophylls purified from oxygenic photosynthetic organisms: effect of the formyl groups conjugated directly to the chlorin pi-macrocycle.
Y. Hirai (2009)
10.1023/A:1006456310193
Chlorophyll breakdown in oilseed rape
S. Hörtensteiner (2004)
Long Term Preservation of the Pressed Leaves by Boiling and Inorganic Salts Treatment
Mi-Soon Byun (2003)
10.1016/j.bmc.2010.06.019
Comparison of demetalation properties between zinc chlorin and zinc porphyrin derivatives: effect of macrocyclic structures.
Y. Saga (2010)
10.1016/S0176-1617(98)80124-7
Chlorophyll breakdown in senescing barley leaves as correlated with phaeophorbidea oxygenase activity
S. Rodoni (1998)
10.1016/J.FOODCHEM.2009.10.056
Impact of UV-B irradiation on chlorophyll degradation and chlorophyll-degrading enzyme activities in stored broccoli (Brassica oleracea L. Italica Group) florets
Sukanya Aiamla-or (2010)
10.2503/JJSHS.67.651
A Possible Participation of Pyropheophorbide a in Chlorophyll Catabolism during Ripening of Citrus unshiu Fruits
H. Kurata (1998)
10.2503/JJSHS.68.817
A Novel Pathway of Chlorophyll Catabolism in Citrus unshiu Fruit : Enzymatic Conversion of Chlorophyllide α to Mg-C132-carboxyl-pyropheophorbide
H. Kurata (1999)
10.1016/J.POSTHARVBIO.2017.02.003
Role of UV-B irradiation dose and intensity on color retention and antioxidant elicitation in broccoli florets (Brassica oleracea var. Italica)
M. Darré (2017)
Lineare Tetrapyrrole in marinen Sedimenten - Untersuchungen zu Struktur und Vorkommen
Vom Fachbereich Chemie (2000)
10.1073/pnas.2036571100
Chlorophyll breakdown: Pheophorbide a oxygenase is a Rieske-type iron–sulfur protein, encoded by the accelerated cell death 1 gene
Adriana Pružinská (2003)
10.1002/cbdv.201100435
Demetalation of Chlorophyll Pigments
Y. Saga (2012)
10.1007/s00425-018-2946-2
Characterization of the pheophorbide a oxygenase/phyllobilin pathway of chlorophyll breakdown in grasses
A. Das (2018)
10.1071/PP00081
Chlorophyll degradation in heat-treated Chlorella pyrenoidosa. A flow cytometric study
Shainnfer Tzeng (2001)
10.1016/S0023-6438(03)00065-3
Effect of immersion solutions on shelf-life of minimally processed lettuce
M. Ihl (2003)
10.2503/JJSHS.67.261
In vitro studies on ethylene-enhanced chlorophyll degrading peroxidase and its reaction products in banana (Musa sapientum L.) fruits
X. Ma (1998)
10.2503/JJSHS.68.825
Ethylene-enhanced Chlorophyll Catabolism in Citrus unshiu Fruit : Effects of Inhibitors on the Activity of Enzymes Involved in Type I Reactions
H. Kurata (1999)
10.1104/pp.105.065870
Chlorophyll Breakdown in Senescent Arabidopsis Leaves. Characterization of Chlorophyll Catabolites and of Chlorophyll Catabolic Enzymes Involved in the Degreening Reaction1
Adriana Pružinská (2005)
10.1111/j.1751-1097.2009.00580.x
Physicochemical Studies of Demetalation of Light‐harvesting Bacteriochlorophyll Isomers Purified from Green Sulfur Photosynthetic Bacteria
Y. Hirai (2009)
10.1016/0031-9422(95)00708-3
Role of chlorophyllase in chlorophyll metabolism in olives cv. Gordal
M. I. Mínguez-Mosquera (1996)
10.1201/9780824746728.ch23
Photosynthetic Pigment Metabolism in Plants During Stress
M. Bertrand (2006)
Running title: ALA Synthesis in the Dark
Bernhard Grimm (2010)
10.1016/S0176-1617(11)81764-5
Breakdown of Chlorophylls by Soluble Proteins Extracted from Leaves of Chenopodium album
Y. Shioi (1995)
10.1034/J.1399-3054.1999.100105.X
Chlorophyll catabolism and gene expression in the peel of ripening banana fruits
R. Drury (1999)
10.1023/B:PHYT.0000047796.98784.06
Peroxidase-mediated chlorophyll degradation in horticultural crops
N. Yamauchi (2004)
Analysis of the AtMRS2 magnesium transporter family in Arabidopsis thaliana via gene-GFP fusions, heterologous complementations, and protein interaction studies
Universitäts und Landesbibliothek Bonn (2007)
10.1111/J.1469-8137.1995.TB04294.X
Chlorophyll breakdown in senescent leaves: identification of the biochemical lesion in a stay-green genotype of Festuca pratensis Huds.
F. Vicentini (1995)
10.1371/journal.pone.0056345
Nitric Oxide Deficiency Accelerates Chlorophyll Breakdown and Stability Loss of Thylakoid Membranes during Dark-Induced Leaf Senescence in Arabidopsis
Fang Liu (2013)
10.1016/1011-1344(95)07257-8
Chlorophyll catabolism — structures, mechanisms, conversions
A. Gossauer (1996)
See more
Semantic Scholar Logo Some data provided by SemanticScholar