Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

The Interaction Of Phosphatidylcholine Bilayers With Triton X-100.

F. Goñi, M. Urbaneja, J. Arrondo, A. Alonso, A. Durrani, D. Chapman
Published 1986 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
The interaction of multilamellar phosphatidylcholine vesicles with the non-ionic detergent Triton X-100 has been studied under equilibrium conditions, specially in the sub-lytic range of surfactant concentrations. Equilibrium was achieved in less than 24 h. Estimations of detergent binding to bilayers, using [3H]Triton X-100, indicate that the amphiphile is incorporated even at very low concentrations (below its critical micellar concentration); a dramatic increase in the amount of bound Triton X-100 occurs at detergent concentrations just below those producing membrane solubilization. Solubilization occurs at phospholipid/detergent molar ratios near 0.65 irrespective of lipid concentration. The perturbation produced by the surfactant in the phospholipid bilayer has been studied by differential scanning calorimetry, NMR and Fourier-transform infrared spectroscopy. At low detergent concentration (lipid/detergent molar ratios above 3), a reduction in 2H-NMR quadrupolar splitting occurs, suggesting a decrease in the static order of the acyl chains; the same effect is detected by Fourier-transform infrared spectroscopy in the form of blue shifts of the methylene stretching vibration bands. Simultaneously, the enthalpy variation of the main phospholipid phase transition is decreased by about a third with respect to its value in the pure lipid/water system. For phospholipid/detergent molar ratios between 3 and 1, the decrease in lipid static order does not proceed any further; rather an increase in fluidity is observed, characterized by a marked decrease in the midpoint transition temperature of the gel-to-fluid phospholipid transition. At the same time an isotropic component is apparent in both 31P-NMR and 2H-NMR spectra, and a new low-temperature endotherm is detected in differential scanning calorimetric traces. When phospholipid and Triton X-100 are present at equimolar ratios some bilayer structure persists, as judged from calorimetric observations, but NMR reveals only one-component isotropic signals. At lipid/detergent molar ratios below unity, the NMR lines become narrower, the main (lamellar) calorimetric endotherm tends to vanish and solubilization occurs.
This paper references
Solubilization of phospholipids by detergents. Structural and kinetic aspects.
D. Lichtenberg (1983)
Lysis and reassembly of sonicated lecithin vesicles in the presence of triton X‐100
A. Alonso (1981)
The phase transition of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine as seen by Fourier transform infrared difference spectroscopy.
D. Cameron (1978)
Lipid polymorphism and the functional roles of lipids in biological membranes.
P. Cullis (1979)
Interactions between sarcoplasmic reticulum calcium adenosintriphosphatase and nonionic detergents.
W. L. Dean (1981)
Solubilization of phosphatidylcholine bilayers by octyl glucoside.
M. Jackson (1982)
Solubilization of the Semliki Forest virus membrane with sodium dodecyl sulfate.
R. Becker (1975)
Review LetterIntrinsic protein—lipid interactions: Physical and biochemical evidence
D. Chapman (1979)
Phosphorus NMR analysis of phospholipids in detergents.
E. London (1979)
Phase behavior of large unilamellar vesicles composed of synthetic phospholipids.
R. Parente (1984)
Characterization of the pretransition in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine by Fourier transform infrared spectroscopy.
D. Cameron (1980)
Nuclear magnetic resonance investigation of the cytochrome oxidase--phospholipid interaction: a new model for boundary lipid.
S. Kang (1979)
Phospholipid phase transitions. Effects of n-alcohols, n-monocarboxylic acids, phenylalkyl alcohols and quaternary ammonium compounds.
A. W. Eliasz (1976)
31P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes
M. Roux (1984)
Intrinsic protein-lipid interactions. Infrared spectroscopic studies of gramicidin A, bacteriorhodopsin and Ca2+-ATPase in biomembranes and reconstituted systems.
M. Cortijo (1982)
Difference infrared spectroscopy of aqueous model and biological membranes using an infrared data station.
D. Chapman (1980)
Intrinsic protein—lipid interactions
D. Chapman (1979)
Unusual enthalpy changes which accompany the titration of dimyristoylphosphatidylcholine vesicles with Triton X-100.
G. Kresheck (1983)
Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies.
B. D. Ladbrooke (1969)
Phase transitions and fluidity characteristics of lipids and cell membranes.
D. Chapman (1975)
Lipid conformation in model membranes and biological membranes.
J. Seelig (1980)
Formation and characterization of mixed micelles of the nonionic surfactant Triton X-100 with egg, dipalmitoyl, and dimyristoyl phosphatidylcholines.
E. Dennis (1974)
31P nuclear magnetic resonance and the head group structure of phospholipids in membranes.
J. Seelig (1978)
Incorporation of bile acid of low concentration into model and biological membranes studied by 2H and 31P NMR.
H. Saito (1983)
Solubilization of membranes by detergents.
A. Helenius (1975)
Structural and kinetic studies on the solubilization of lecithin by sodium deoxycholate.
D. Lichtenberg (1979)
Membrane-surfactant interactions. The effect of Triton X-100 on sarcoplasmic reticulum vesicles.
A. Prado (1983)
Phosphorus assay in column chromatography.
G. R. Bartlett (1959)
Phosphorus-31 nuclear magnetic resonance spectra characteristic of hexagonal and isotropic phospholipid phases generated from phosphatidylethanolamine in the bilayer phase.
A. Thayer (1981)
Deuterium nuclear magnetic resonance studies of bile salt/phosphatidylcholine mixed micelles.
R. Stark (1983)

This paper is referenced by
Interaction of N,N,N-trialkylammonioundecahydro-closo-dodecaborates with dipalmitoyl phosphatidylcholine liposomes.
Tanja Schaffran (2010)
Monitoring detergent-mediated solubilization and reconstitution of lipid membranes by isothermal titration calorimetry
H. Heerklotz (2009)
Solubilization of unilamellar liposomes by betaine-type zwitterionic/anionic surfactant systems
A. D. L. Maza (1995)
IR spectroscopy analysis of pancreatic lipase-related protein 2 interaction with phospholipids: 1. Discriminative recognition of mixed micelles versus liposomes.
E. Mateos-Díaz (2018)
Phospholipid vesicle solubilization and reconstitution by detergents. Symmetrical analysis of the two processes using octaethylene glycol mono-n-dodecyl ether.
D. Levy (1990)
Quantitative evaluation of human leukocyte interferon-α entrapped in liposomes
C. Karau (1996)
Membrane protein reconstitution into liposomes guided by dual-color fluorescence cross-correlation spectroscopy.
Peter I. Simeonov (2013)
Preparation of an electrochemical biosensor based on lipid membranes in nanoporous alumina.
Jean-Baptiste Largueze (2010)
Magnetically-orientable Tween-based model membranes for NMR studies of proteins.
Andrée E. Gravel (2020)
Unveiling the multi-step solubilization mechanism of sub-micron size vesicles by detergents
P. Dalgarno (2019)
Biosensors based on release of compounds upon disruption of lipid bilayers supported on porous microspheres
Menake E. Piyasena (2008)
Solubilization of phospholipid bilayers by C14-alkyl betaine/anionic mixed surfactant systems
A. Maza (1995)
Preparation and characterization of reactive and stable glucose oxidase-containing liposomes modulated with detergent.
M. Yoshimoto (2003)
Study of rabbit erythrocytes membrane solubilization by sucrose monomyristate using laurdan and phasor analysis.
G. Günther (2018)
Structural changes induced by Triton X-100 on sonicated phosphatidylcholine liposomes.
M. Urbaneja (1988)
Solubilizing effects caused by alkyl pyridinium surfactants in phosphatidylcholine liposomes.
A. de la Maza (1995)
Permeability changes in the phospholipid bilayer caused by nonionic surfactants
A. Maza (1992)
Interaction of Triton X-100 and octyl glucoside with liposomal membranes at sublytic and lytic concentrations. Spectroscopic studies.
J. Lasch (1990)
Detergent-resistant membranes should not be identified with membrane rafts.
D. Lichtenberg (2005)
Interactions of surfactants with lipid membranes.
H. Heerklotz (2008)
Biomembrane solubilization mechanism by Triton X-100: a computational study of the three stage model.
A. Pizzirusso (2017)
Interaction of membrane proteins and lipids with solubilizing detergents.
M. le Maire (2000)
Solubilization of phospholipid bilayer caused by surfactants
A. Maza (1993)
Sphingosine increases the permeability of model and cell membranes.
F. Contreras (2006)
Detergent solubilisation of phospholipid bilayers in the gel state: the role of polar and hydrophobic forces.
S. Patra (1998)
An assessment of the biochemical applications of the non-ionic surfactant Hecameg.
M. Begoña Ruiz (1994)
Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization.
P. Chae (2013)
Induction of conductance heterogeneity in gramicidin channels.
D. B. Sawyer (1989)
On the effect of lysophosphatidylcholine, platelet activating factor and other surfactants on calcium permeability in sarcoplasmic reticulum vesicles.
J. A. Teruel (1991)
IR spectroscopy analysis of pancreatic lipase-related protein 2 interaction with phospholipids: 2. Discriminative recognition of various micellar systems and characterization of PLRP2-DPPC-bile salt complexes.
E. Mateos-Díaz (2018)
Spectroscopic techniques in the study of membrane solubilization, reconstitution and permeabilization by detergents.
F. Goñi (2000)
Membrane proteins, lipids and detergents: not just a soap opera.
A. Seddon (2004)
See more
Semantic Scholar Logo Some data provided by SemanticScholar