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Primary Structure Of Human Placental 5'-nucleotidase And Identification Of The Glycolipid Anchor In The Mature Form.
Y. Misumi, S. Ogata, K. Ohkubo, S. Hirose, Y. Ikehara
Published 1990 · Biology, Medicine
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A cDNA was cloned coding for human placental 5'-nucleotidase. The 3547-bp cDNA contains an open reading frame that encodes a 574-residue polypeptide with calculated size of 63 375 Da. The NH2-terminal 26 residues comprise a signal peptide, which is followed by the NH2-terminal sequence of the purified protein. four potential N-linked glycosylation sites are found in the molecule, accounting for a larger mass of the mature form (71 kDa). The predicted structure contains a hydrophobic amino acid sequence at the COOH terminus, a possible signal for the post-translational modification by glycophospholipid. To confirm this possibility, we tried to isolate and characterize the membrane-anchoring domain of 5'-nucleotidase. BrCN-cleaved fragments of the protein were extracted with hexane and subjected to HPLC, resulting in purification of a single component of 2.3 kDa. Chemical analyses revealed that the purified fragment contains the tetradecapeptide Lys-Val-Ile-Tyr-Pro-Ala-Val-Glu-Gly-Arg-Ile-Lys-Phe-Ser, ethanolamine, glucosamine, mannose, inositol, palmitic acid, and stearic acid. The peptide sequence determined is identified at positions 510-523 in the primary structure deduced from the cDNA sequence, which predicts a further extension to position 548, containing the hydrophobic amino acid sequence. Thus, it is concluded that the mature 5'-nucleotidase lacks the predicted COOH-terminal peptide extension (524-548), which has been replaced by the glycophospholipid functioning as the membrane anchor of 5'-nucleotidase.
This paper references
What do ectoenzymes do
K. K. Stanley (1982)
Aspartic acid-484 of nascent placental alkaline phosphatase condenses with a phosphatidylinositol glycan to become the carboxyl terminus of the mature enzyme.
R. Micanovic (1988)
GENAS: a database system for nucleic acid sequence analysis
S. Kuhara (1984)
Slab gel system for the resolution of oligopeptides below molecular weight of 10,000.
F. Burr (1983)
Phosphatidyl inositol-specific phospholipase C from Clostridium novyi type A.
R. Taguchi (1978)
Simplified preparation of unidirectional deletion clones.
J. Hoheisel (1986)
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
M. Kozak (1987)
The sucrase-isomaltase complex: Primary structure, membrane-orientation, and evolution of a stalked, intrinsic brush border protein
W. Hunziker (1986)
A simple and very efficient method for generating cDNA libraries.
U. Gubler (1983)
Gas Chromatographic Analysis of Aldoses
T. Imanari (1969)
Primary structure of rat liver dipeptidyl peptidase IV deduced from its cDNA and identification of the NH2-terminal signal sequence as the membrane-anchoring domain.
S. Ogata (1989)
The glycosyl-phosphatidylinositol anchor of membrane proteins.
M. Low (1989)
Identification of amine components in a glycolipid membrane-binding domain at the C-terminus of human erythrocyte acetylcholinesterase.
R. Haas (1986)
Multiple Forms of Rat‐Serum α1‐Protease Inhibitor
Y. Ikehara (1981)
The MRC OX‐45 antigen of rat leukocytes and endothelium is in a subset of the immunoglobulin superfamily with CD2, LFA‐3 and carcinoembryonic antigens.
N. Killeen (1988)
Transport and metabolism of 5'-nucleotidase in a rat hepatoma cell line.
R. A. van den Bosch (1986)
Amino acid sequence of rabbit kidney neutral endopeptidase 24.11 (enkephalinase) deduced from a complementary DNA.
A. Devault (1987)
A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides.
D. Perlman (1983)
Primary structure of rat liver alkaline phosphatase deduced from its cDNA.
Y. Misumi (1988)
Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures.
M. A. Ferguson (1988)
Chemical characterization of the membrane-anchoring domain of human placental alkaline phosphatase.
S. Ogata (1988)
Primary structure of rat liver 5'-nucleotidase deduced from the cDNA. Presence of the COOH-terminal hydrophobic domain for possible post-translational modification by glycophospholipid.
Y. Misumi (1990)
Anchoring and biosynthesis of stalked brush border membrane proteins: glycosidases and peptidases of enterocytes and renal tubuli.
G. Semenza (1986)
Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein.
M. Ferguson (1985)
Myristylation of the membrane form of a Trypanosoma brucei variant surface glycoprotein.
M. Ferguson (1984)
A glycophospholipid tail at the carboxyl terminus of the Thy-1 glycoprotein of neurons and thymocytes.
A. Tse (1985)
Efficient isolation of genes by using antibody probes.
R. Young (1983)
Chemical identification of lipid components in the membranous form of rat liver alkaline phosphatase.
S. Ogata (1987)
Patterns of Amino Acids near Signal‐Sequence Cleavage Sites
G. Heijne (1983)
Structural characterization of the glycoinositol phospholipid membrane anchor of human erythrocyte acetylcholinesterase by fast atom bombardment mass spectrometry.
W. Roberts (1988)
Oncodevelopmental expression and structure of alkaline phosphatase genes.
Millán Jl (1988)
A simple method for displaying the hydropathic character of a protein.
J. Kyte (1982)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
A biochemical dissection of the functional polarity of the plasma membrane of the hepatocyte
W. H. Evans (1980)
Molecular cloning and nucleotide sequence of rat kidney gamma-glutamyl transpeptidase cDNA.
Y. Laperche (1986)
Ecto-5'-nucleotidase does not catalyze vectorial production of adenosine in the perfused rat liver.
Terukatsu Sasaki (1983)
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
B. Oakley (1980)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
DNA sequencing with chain-terminating inhibitors.
F. Sanger (1977)
Specific release of plasma membrane enzymes by a phosphatidylinositol-specific phospholipase C.
M. Low (1978)
Purification of 5'-nucleotidase from human placenta after release from plasma membranes by phosphatidylinositol-specific phospholipase C.
L. Thompson (1987)
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Fasciola gigantica: purification and characterization of adenosine deaminase.
E. M. Ali (2008)
Characterization of an ecto-5'-nucleotidase (EC 22.214.171.124) activity in intact trophozoites of Trichomonas gallinae.
F. P. Borges (2007)
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E. Kaniewska (2014)
Contribution of ecto-5'-nucleotidase to the inhibition of platelet aggregation by human endothelial cells.
Y. Kawashima (2000)
The Role of Purinergic Signalling in Inflammatory Disorders
N. J. Geraghty (2019)
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N. Shimada (2001)
Adult Stem Cell Membrane Markers: Their Importance and Critical Role in Their Proliferation and Differentiation Potentials
M. Garza (2018)
Therapeutic Potentials of Ecto‐Nucleoside Triphosphate Diphosphohydrolase, Ecto‐Nucleotide Pyrophosphatase/Phosphodiesterase, Ecto‐5′‐Nucleotidase, and Alkaline Phosphatase Inhibitors
Mariya al-Rashida (2014)
THE MOLECULAR BIOLOGY OF GPI-ANCHORED BORDER HYDROLASES
A. Turner (1992)
Downstream Pathways of Adenosine
A. Sebastião (2013)
Regional localization of a trophoblast antigen-related sequence and 16 other sequences to human chromosomes 6q using somatic cell hybrids.
J. Boyle (1992)
A Deep Insight into the Sialotranscriptome of the Gulf Coast Tick, Amblyomma maculatum
S. Karim (2011)
CD38: a new paradigm in lymphocyte activation and signal transduction
F. Lund (1998)
Expression of high Km 5'-nucleotidase in leukemic blasts is an independent prognostic factor in adults with acute myeloid leukemia.
C. Galmarini (2001)
Identification of novel chromone based sulfonamides as highly potent and selective inhibitors of alkaline phosphatases.
Mariya al-Rashida (2013)
The dephosphorylation of AMP and IMP by a soluble low Km 5'nucleotidase from human seminal plasma: some regulatory aspects.
A. Minelli (1995)
Protein Anchoring to Membrane by Glycosylphosphatidylinositol. Determination of the COOH-Terminal Signal Peptide Sequence and GPI-Attachment Site in Bovine Liver 5′-Nucleotidase
H. Ikezawa (1993)
BIOCHEMISTRY, LOCALIZATION AND FUNCTIONAL ROLES OF ECTO-NUCLEOTIDASES IN THE NERVOUS SYSTEM
H. Zimmermann (1996)
The architecture and interactions of leucocyte surface molecules
A. Barclay (1997)
An annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*
J. Ribeiro (2007)
Prediction of potential GPI-modification sites in proprotein sequences.
B. Eisenhaber (1999)
The role of 5'-nucleotidases and Deoxynucleoside Kinases in Responses to Nucleoside Analogues
Saeedeh Mirzaee (2010)
5'nucleotidase is sorted to the apical domain of hepatocytes via an indirect route [published erratum appears in J Cell Biol 1993 Nov;123(3):following 767]
M. Schell (1992)
5'-nucleotidase from the electric ray electric lobe. Primary structure and relation to mammalian and procaryotic enzymes.
W. Volknandt (1991)
Hyperuricemia and Gout as Metabolic Disorders, and Purine Nucleotide and Uric Acid Metabolism
J. Oka (2005)
Mass spectrometry study of ecto-5'-nucleotidase from bull seminal plasma.
C. Fini (2000)
EFEITOS DE CRISES CRÔNICAS E AGUDAS INDUZIDAS POR PENTILENOTETRAZOL SOBRE A HIDRÓLISE DE NUCLEOTÍDEOS PÚRICOS EM SORO DE RATOS ADULTOS
A. Bruno (2002)
5'-nucleotidase--an overview of the last three years.
L. Thompson (1991)
T cell signalling through CD73.
R. Resta (1997)
Nucleotidases and Nucleoside Analog Cytotoxicity
S. Hunsucker (2006)
Correction: NT5E Mutations That Cause Human Disease Are Associated with Intracellular Mistrafficking of NT5E Protein
Michel Fausther (2015)
Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene.
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