Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN LUNG TISSUE USING A FIBER OPTIC BIOSENSOR

Kimberly A. Denton, M. F. Kramer, D. Lim
Published 2009 · Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
ABSTRACT There is no rapid diagnostic technique at medical examiners' offices to determine if a decedent is infected with Mycobacterium tuberculosis. Present diagnostic testing requires at least 1 month for results. The RAPTOR, a portable, automated fiber optic evanescent wave biosensor, was used as the platform to develop more rapid assays to detect M. tuberculosis from lung tissue. Positive biosensor detection was obtained 80% of the time at cell concentrations of 106 cells/mL, 96% of the time at 107 cells/mL, and 99% of the time at 108 cells/mL of live attenuated M. tuberculosis (ATCC 25177) suspended in phosphate-buffered saline with 0.1% Tween 20 (PBST). Live attenuated M. tuberculosis suspended in PBST and seeded into decedent lung tissue was tested using the RAPTOR. Positive detection was obtained 96% of the time at 107 cells/mL and 100% of the time at 108 cells/mL. Detection of M. tuberculosis in lung tissue homogenate was possible within 3 h. PRACTICAL APPLICATIONS Development of a rapid method to detect Mycobacterium tuberculosis from lung tissue at autopsy would be a significant benefit to medical examiners' offices and pathologists worldwide. Rapid diagnosis would enable those possibly exposed to an infected decedent to be informed of M. tuberculosis infection in a timely manner. Biosensor assays on seeded lung tissue show it is possible to detect M. tuberculosis in 3 h using the RAPTOR biosensor, instead of 1 month using current, conventional tests, when there are 107 or more cells in the assay sample. Tuberculous cavities may contain 107–109 activelymultiplying organisms. Sample preparation and assay protocols involve common laboratory practices and could easily and quickly be learned and implemented by laboratory staff. The RAPTOR is user friendly and could be used by medical examiner staff at time of need. The biosensor assay may also have potential applications for use on biopsy tissue from living individuals who have heavy pulmonary or disseminated infections by M. tuberculosis.
This paper references
10.1016/S0366-0850(39)80016-3
The evidence for the incidence of tuberculosis in ancient Egypt
A. Cave (1939)
10.1128/JCM.30.9.2476-2478.1992
Evaluation of nonradioactive DNA probes for identification of mycobacteria.
L. Lebrun (1992)
Genomic analysis reveals variation between Mycobacterium tuberculosis H37Rv and the attenuated M. tuberculosis H37Ra strain.
Roland Brosch (1999)
10.1016/J.HUMPATH.2005.04.010
Survival of Mycobacterium tuberculosis organisms for 8 days in fresh lung tissue from an exhumed body.
K. Nolte (2005)
10.17226/10045
Tuberculosis in the Workplace
M. J. Field (2001)
10.7326/0003-4819-119-5-199309010-00009
Tuberculosis in the 1990s
P. F. Barnes (1993)
10.1001/JAMA.282.7.677
Consensus statement. Global burden of tuberculosis: estimated incidence, prevalence, and mortality by country. WHO Global Surveillance and Monitoring Project.
C. Dye (1999)
10.1093/nar/18.1.188
IS6110, an IS-like element of Mycobacterium tuberculosis complex
D. Thierry (1990)
10.1213/00000539-195101000-00003
The Merck Manual of Diagnosis and Therapy
M. Beers (1982)
10.1172/JCI11426
The nature and consequence of genetic variability within Mycobacterium tuberculosis.
M. Kato-Maeda (2001)
10.1016/J.BIOS.2005.02.012
Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection.
Joyce M. Simpson (2005)
10.1056/NEJM199608293350918
Transmission of tuberculosis during a long airplane flight.
Tracy Mj (1996)
10.1109/JPROC.2003.813574
Detection of microorganisms and toxins with evanescent wave fiber-optic biosensors
D. Lim (2003)
Mycobacterium - Manual of Clinical Microbiology
G. Roberts (1991)
10.1111/J.1745-4581.2001.TB00250.X
DIRECT DETECTION OF ESCHERICHIA COLI 0157:H7 IN UNPASTEURIZED APPLE JUICE WITH AN EVANESCENT WAVE BIOSENSOR
D. R. Demarco (2001)
10.1128/JCM.40.5.1873-1874.2002
Tetrazolium Microplate Assay as a Rapid and Inexpensive Colorimetric Method for Determination of Antibiotic Susceptibility of Mycobacterium tuberculosis
L. Caviedes (2002)
10.1001/JAMA.1946.02870480024007
Treatment of tuberculosis with streptomycin; a summary of observations on one hundred cases.
H. C. Hinshaw (1946)
10.1007/3-540-57856-0_1
Personal Communications
D. Goodman (1994)
10.1016/S0167-7012(03)00133-7
Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection.
T. B. Tims (2003)
Todar's Online Textbook of Bacteriology
H. Darmani (2006)
La vaccination preventive contre la tuberculose par le "BCG"
A. Calmette (1927)
Identification of Mycobacterium and Mycobacterium tuberculosis
Sosnovskaia Av (1991)
Evaluation of FASTPlaqueTB-RIF, a rapid, manual test for the determination of rifampicin resistance from Mycobacterium tuberculosis cultures.
H. Albert (2001)
10.1007/BF02867359
Immunodiagnosis of tuberculosis: An update
A. S. Bhatia (2008)
Infectious diseases caused by mycobacteria
M. Beers (2001)
Medical Laboratory Scientist IV. Florida Department of Health, Bureau of Laboratories, Mycobacteriology Laboratory. Jacksonville, Florida
S. Dean (2005)
10.1201/b13378-9
Introduction to Microbiology
J. Ingraham (1994)
10.1128/JCM.28.6.1204-1213.1990
Specific detection of Mycobacterium tuberculosis complex strains by polymerase chain reaction.
P. Hermans (1990)
The combatting of tuberculosis in the light of experience that has been gained in the successful combatting of other infectious diseases
R. Koch (1901)
10.1080/027868299304750
Detection and Characterization of Airborne Mycobacterium tuberculosis H37Ra Particles, A Surrogate for Airborne Pathogenic M. tuberculosis
M. Schafer (1999)
10.3201/eid0811.020291
Molecular Differentiation of Mycobacterium tuberculosis Strains without IS6110 Insertions
K. Lok (2002)
10.1128/JCM.25.8.1551-1552.1987
Rapid identification using a specific DNA probe of Mycobacterium avium complex from patients with acquired immunodeficiency syndrome.
T. Kiehn (1987)
10.1056/nejm199902043400507
Tuberculosis in patients with human immunodeficiency virus infection.
D. Havlir (1999)
10.1038/NM0497-465
Evaluation of a new rapid bacteriophage-based method for the drug susceptibility testing of Mycobacterium tuberculosis
S. Wilson (1997)
10.1378/CHEST.119.2.640
Transmission of Mycobacterium tuberculosis to a funeral director during routine embalming.
M. Lauzardo (2001)
10.1128/JCM.32.1.262-266.1994
Stability of DNA fingerprint pattern produced with IS6110 in strains of Mycobacterium tuberculosis.
M. D. Cave (1994)
10.1128/JCM.25.8.1442-1445.1987
Rapid identification of Mycobacterium avium complex in culture using DNA probes.
T. Drake (1987)
Microbiology. 3 Ed
D. Lim (2003)
10.1111/J.1745-4581.2002.TB00015.X
RECOVERY OF ESCHERICHIA COLI O157:H7 FROM FIBER OPTIC WAVEGUIDES USED FOR RAPID BIOSENSOR DETECTION
M. F. Kramer (2002)
10.1007/978-1-349-09071-6_2
Introduction to Microbiology
K. Ellis (1989)
10.7326/0003-4819-122-12-199506150-00005
The Risk for Transmission of Mycobacterium tuberculosis at the Bedside and during Autopsy
G. Templeton (1995)
10.1111/J.1198-743X.2004.00825.X
Multicentre quality control study for detection of Mycobacterium tuberculosis in clinical samples by nucleic amplification methods.
G. T. Noordhoek (2004)
10.1128/JCM.39.7.2418-2424.2001
Combinatorial Use of Antibodies to Secreted Mycobacterial Proteins in a Host Immune System-Independent Test for Tuberculosis
C. P. Landowski (2001)
10.4315/0362-028X-65.4.596
Detection of Escherichia coli O157:H7 in 10- and 25-gram ground beef samples with an evanescent-wave biosensor with silica and polystyrene waveguides.
D. R. Demarco (2002)
10.1056/NEJM199604113341501
Transmission of multidrug-resistant Mycobacterium tuberculosis during a long airplane flight.
T. Kenyon (1996)
Bacteriologic basis of short-course chemotherapy for tuberculosis.
J. Grosset (1980)
10.1128/CMR.18.4.583-607.2005
Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare
D. Lim (2005)
10.1136/jcp.2005.032276
Mycobacterium tuberculosis at autopsy—exposure and protection: an old adversary revisited
R. Flavin (2007)
10.1128/JCM.32.12.3018-3025.1994
Restriction fragment length polymorphism Mycobacterium tuberculosis strains isolated from Greenland during 1992: evidence of tuberculosis transmission between Greenland and Denmark.
Z. H. Yang (1994)
Test on a new vascular radiology
M. Abreu (1926)
10.1128/9781555817657
Tuberculosis and the tubercle bacillus.
S. Cole (2005)
10.1128/JCM.13.5.899-907.1981
Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria.
G. O. Guerrant (1981)
10.1073/PNAS.0602606103
Toward the structural genomics of complexes: crystal structure of a PE/PPE protein complex from Mycobacterium tuberculosis.
Michael Strong (2006)
History of the Sanatorium Movement
A. Davis (1996)
10.1016/0890-8508(91)90040-Q
IS6110: conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting.
M. D. Cave (1991)
10.7326/0003-4819-101-2-288_2
Color atlas and textbook of diagnostic microbiology
E. Koneman (1983)
10.1001/JAMA.282.7.677
Global Burden of Tuberculosis: Estimated Incidence, Prevalence, and Mortality by Country
C. Dye (1999)
10.1128/JCM.35.1.121-124.1997
Clinical evaluation of difco ESP culture system II for growth and detection of mycobacteria.
G. Woods (1997)
10.1164/AJRCCM/141.2.347
Extrapulmonary tuberculosis in the United States.
H. Rieder (1990)
10.1056/NEJM199101313240503
Treatment of tuberculosis in patients with advanced human immunodeficiency virus infection.
P. Small (1991)
10.2741/A285
Mycobacterial pathogenesis: a historical perspective.
F. M. Collins (1998)
10.1128/JCM.31.7.1777-1782.1993
Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization.
F. Nolte (1993)
10.1164/ARRD.1982.125.3P2.8
Epidemiology of tuberculosis.
G. Comstock (1982)
10.1017/s0195941700007311
Transmission of multidrug-resistant Mycobacterium tuberculosis among persons exposed in a medical examiner's office, New York.
X. T. Ussery (1995)
10.1109/51.294007
Development of an evanescent wave fiber optic biosensor
G. Anderson (1994)
10.1084/JEM.57.2.239
BIOLOGICAL STUDIES OF THE TUBERCLE BACILLUS : II. A NEW CONCEPTION OF THE PATHOLOGY OF EXPERIMENTAL AVIAN TUBERCULOSIS WITH SPECIAL REFERENCE TO THE DISEASE PRODUCED BY DISSOCIATED VARIANTS.
W. A. Winn (1933)
10.1128/JCM.29.11.2473-2476.1991
Evaluation of acridinium-ester-labeled DNA probes for identification of Mycobacterium tuberculosis and Mycobacterium avium-Mycobacterium intracellulare complex in culture.
M. Goto (1991)
10.1093/OXFORDJOURNALS.AJE.A112675
Extrapulmonary tuberculosis in the United States.
L. Farer (1979)
10.1016/J.MIMET.2007.11.004
Monitoring biosensor capture efficiencies: development of a model using GFP-expressing Escherichia coli O157:H7.
Joyce M Simpson-Stroot (2008)
10.5588/09640569513048
Rapid indication of multidrug-resistant tuberculosis from liquid cultures using FASTPlaqueTB-RIF, a manual phage-based test.
H. Albert (2002)
10.1016/0022-1759(85)90354-0
An improved method of antigen detection on nitrocellulose: in situ staining of alkaline phosphatase conjugated antibody.
M. Dao (1985)
10.1128/JCM.18.3.689-696.1983
Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens.
G. Roberts (1983)
10.1099/JMM.0.05454-0
Preliminary study on rapid identification of Mycobacterium tuberculosis complex isolates by the BD ProbeTec ET system.
S. Wang (2004)
10.1038/2061272B0
Identification of Bacterial Strains by Pyrolysis Gas-liquid Chromatography
E. Reiner (1965)
Tuberculosis, Man and Society: The White Plague
R. Dubos (1952)
10.1016/J.HUMPATH.2004.01.009
Viability of mycobacteria in formalin-fixed lungs.
K. F. Gerston (2004)
10.1007/BF01765224
Die Aetiologie der Tuberkulose
R. Koch (2005)
Reported tuberculosis in the United States, 2010
L. Armstrong (2004)
10.1007/PL00015025
Simple Procedure for Drug Susceptibility Testing of Mycobacterium tuberculosis Using a Commercial Colorimetic Assay
J. C. Palomino (1999)
10.1097/00000433-200206000-00001
Biosafety Considerations for Autopsy
K. Nolte (2002)
Evaluation of FASTPlaqueTB-RIF for determination of rifampicin resistance in Mycobacterium tuberculosis complex isolates.
O. Kisa (2003)
10.1055/s-0029-1196567
Die Aetiologie der Tuberculose
H. Ziemssen (1887)
Genomic Analysis Reveals Variation between Mycobacterium tuberculosis H37Rv and the Attenuated M. tuberculosis H37Ra Strain
A. Ende (1999)
Zentralbl. Bakteriol. Parasitenkd
L. Lowenstein (1931)
10.1128/JCM.39.2.651-657.2001
Comparison of MB/BacT ALERT 3D System with Radiometric BACTEC System and Löwenstein-Jensen Medium for Recovery and Identification of Mycobacteria from Clinical Specimens: a Multicenter Study
C. Piersimoni (2001)
Lintradermo-reaction a la tuberculine et son interpretation clinique
C. Mantoux (1910)
10.1128/JCM.10.4.506-514.1979
Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone.
P. A. Tisdall (1979)
10.1128/JCM.40.1.150-154.2002
Multicenter Evaluation of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System for Susceptibility Testing of Mycobacterium tuberculosis
P. Bémer (2002)
10.1164/ARRD.1975.112.6.773
Laboratory services for mycobacterial diseases.
G. Kubica (1975)
10.1084/JEM.60.4.515
BIOLOGICAL STUDIES OF THE TUBERCLE BACILLUS : III. DISSOCIATION AND PATHOGENICITY OF THE R AND S VARIANTS OF THE HUMAN TUBERCLE BACILLUS (H37)
W. Steenken (1934)
Associate Medical Examiner
J. Lee (2005)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar