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Oral Creatine Supplementation Facilitates The Rehabilitation Of Disuse Atrophy And Alters The Expression Of Muscle Myogenic Factors In Humans

P. Hespel, B. Op't Eijnde, Marc Van Leemputte, Birgitte Ursø, P. Greenhaff, V. Labarque, S. Dymarkowski, P. Hecke, Erik A. Richter
Published 2001 · Biology, Medicine

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1 We investigated the effect of oral creatine supplementation during leg immobilization and rehabilitation on muscle volume and function, and on myogenic transcription factor expression in human subjects. 2 A double‐blind trial was performed in young healthy volunteers (n=22). A cast was used to immobilize the right leg for 2 weeks. Thereafter the subjects participated in a knee‐extension rehabilitation programme (3 sessions week−1, 10 weeks). Half of the subjects received creatine monohydrate (CR; from 20 g down to 5 g daily), whilst the others ingested placebo (P; maltodextrin). 3 Before and after immobilization, and after 3 and 10 weeks of rehabilitation training, the cross‐sectional area (CSA) of the quadriceps muscle was assessed by NMR imaging. In addition, an isokinetic dynamometer was used to measure maximal knee‐extension power (Wmax), and needle biopsy samples taken from the vastus lateralis muscle were examined to asses expression of the myogenic transcription factors MyoD, myogenin, Myf5, and MRF4, and muscle fibre diameters. 4 Immobilization decreased quadriceps muscle CSA (∼10 %) and Wmax (∼25 %) by the same magnitude in both groups. During rehabilitation, CSA and Wmax recovered at a faster rate in CR than in P (P < 0.05 for both parameters). Immobilization changed myogenic factor protein expression in neither P nor CR. However, after rehabilitation myogenin protein expression was increased in P but not in CR (P < 0.05), whilst MRF4 protein expression was increased in CR but not in P (P < 0.05). In addition, the change in MRF4 expression was correlated with the change in mean muscle fibre diameter (r=0.73, P < 0.05). 5 It is concluded that oral creatine supplementation stimulates muscle hypertrophy during rehabilitative strength training. This effect may be mediated by a creatine‐induced change in MRF4 and myogenin expression.
This paper references
10.1097/00005768-199908000-00011
Performance and muscle fiber adaptations to creatine supplementation and heavy resistance training.
J. Volek (1999)
10.1002/AJA.1001980307
Differential expression of muscle regulatory factor genes in normal and denervated adult rat hindlimb muscles
S. Voytik (1993)
10.1212/WNL.54.9.1848
Creatine monohydrate in muscular dystrophies: A double-blind, placebo-controlled clinical study
M. Walter (2000)
10.1152/AJPENDO.1994.266.5.E725
Effect of oral creatine supplementation on skeletal muscle phosphocreatine resynthesis.
P. Greenhaff (1994)
10.1073/PNAS.88.4.1349
Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity.
R. Eftimie (1991)
10.1080/00365517409082477
Glycogen, glycolytic intermediates and high-energy phosphates determined in biopsy samples of musculus quadriceps femoris of man at rest. Methods and variance of values.
R. Harris (1974)
10.1152/JAPPL.1999.87.5.1705
Time course of changes in markers of myogenesis in overloaded rat skeletal muscles.
G. Adams (1999)
Selective accumulation of MyoD and myogenin mRNAs in fast and slow adult skeletal muscle is controlled by innervation and hormones.
S. Hughes (1993)
10.1097/00005768-200003000-00024
American College of Sports Medicine roundtable. The physiological and health effects of oral creatine supplementation.
R. Terjung (2000)
10.1007/978-1-4615-5653-4_30
Creatine supplementation in health and disease. Effects of chronic creatine ingestion in vivo: down-regulation of the expression of creatine transporter isoforms in skeletal muscle
Maria Lourdes Guerrero-Ontiveros (1998)
10.1001/ARCHNEUR.57.7.956
Creatine therapy in myophosphorylase deficiency (McArdle disease): a placebo-controlled crossover trial.
M. Vorgerd (2000)
10.1212/WNL.55.11.1748
A placebo-controlled crossover trial of creatine in mitochondrial diseases
T. Klopstock (2000)
10.1177/18.9.670
THREE "MYOSIN ADENOSINE TRIPHOSPHATASE" SYSTEMS: THE NATURE OF THEIR pH LABILITY AND SULFHYDRYL DEPENDENCE
M. Brooke (1970)
Selective accumulation of MyoD and myogenin in fast and slow skeletal muscle is controlled by innervation and hormones
E. HULTMAN (1993)
10.1042/CS0830367
Elevation of creatine in resting and exercised muscle of normal subjects by creatine supplementation.
R. Harris (1992)
10.1139/O95-080
Determination versus differentiation and the MyoD family of transcription factors.
L. Megeney (1995)
10.1083/JCB.145.3.633
Myogenin Induces a Shift of Enzyme Activity from Glycolytic to Oxidative Metabolism in Muscles of Transgenic Mice
S. Hughes (1999)
10.1042/CS0840565
Influence of oral creatine supplementation of muscle torque during repeated bouts of maximal voluntary exercise in man.
P. Greenhaff (1993)
10.1152/JAPPL.1998.84.4.1359
Myogenin, MyoD, and myosin expression after pharmacologically and surgically induced hypertrophy.
P. Mozdziak (1998)
10.1055/S-2000-8848
Dietary creatine monohydrate supplementation increases satellite cell mitotic activity during compensatory hypertrophy.
B. Dangott (2000)
10.1152/JAPPL.1996.80.2.452
Caffeine counteracts the ergogenic action of muscle creatine loading.
K. Vandenberghe (1996)
10.1152/JAPPL.1997.83.6.2055
Long-term creatine intake is beneficial to muscle performance during resistance training.
K. Vandenberghe (1997)
10.2165/00007256-199010010-00005
Muscular Atrophy Following Immobilisation
H. J. Appell (1990)
10.1016/0014-5793(96)00681-3
Two myogenic regulatory factor transcripts exhibit muscle‐specific responses to disuse and passive stretch in adult rats
P. Loughna (1996)
10.1152/JAPPL.1996.81.1.232
Muscle creatine loading in men.
E. Hultman (1996)
10.1111/j.1600-0838.1993.tb00378.x
Creatine supplementation and dynamic high‐intensity intermittent exercise
P. Balsom (1993)
Muscular atrophy following immobilisation . A review
P. D. BALSOM (1990)
10.1152/JAPPL.1997.83.4.1270
Myogenic regulatory factors during regeneration of skeletal muscle in young, adult, and old rats.
D. Marsh (1997)
10.1097/00005768-199801000-00011
Effects of creatine supplementation on body composition, strength, and sprint performance.
R. Kreider (1998)
10.1161/01.CIR.100.18.1847
Downregulation of the Na(+)-creatine cotransporter in failing human myocardium and in experimental heart failure.
S. Neubauer (1999)
10.1212/WNL.52.4.854
Creatine monohydrate increases strength in patients with neuromuscular disease
M. Tarnopolsky (1999)



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10.1519/JSC.0000000000001191
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K. Hackney (2015)
10.1007/s40279-015-0398-4
Nutritional Support for Exercise-Induced Injuries
K. Tipton (2015)
The Relationship Between Endogenous Creatine Levels and Maximal Upper Body Strength, Short Term Muscle Recovery and Body Fat in Males
V. Dalbo (2007)
Acute skeletal muscle wasting in the critically ill
Z. Puthucheary (2014)
10.1007/978-1-59745-573-2_5
Creatine Consumption in Health
J. Poortmans (2008)
10.1152/AJPCELL.00162.2007
Creatine enhances differentiation of myogenic C2C12 cells by activating both p38 and Akt/PKB pathways.
L. Deldicque (2007)
10.1152/PHYSIOLGENOMICS.00060.2002
Effects of creatine supplementation on housekeeping genes in human skeletal muscle using real-time RT-PCR.
R. Murphy (2003)
10.1042/CS20020159
Effects of creatine loading and prolonged creatine supplementation on body composition, fuel selection, sprint and endurance performance in humans.
L. V. van Loon (2003)
10.1249/JSR.0b013e31829cdff2
Creatine Supplementation
M. Hall (2013)
Journal of the International Society of Sports Nutrition International Society of Sports Nutrition Position Stand: Creatine Supplementation and Exercise a Position Statement and Review of the Literature Creatine Supplementation and Exercise: a Review of the Literature
T. Buford ()
10.1007/s00424-007-0423-z
Cellular and molecular events controlling skeletal muscle mass in response to altered use
F. Favier (2007)
10.1007/978-1-59745-573-2_2
Creatine Supplementation in Strength-Power Sports
D. Willoughby (2008)
10.1152/JAPPLPHYSIOL.00014.2003
Acute molecular responses of skeletal muscle to resistance exercise in able-bodied and spinal cord-injured subjects.
C. Bickel (2003)
10.1007/s00421-010-1556-x
Disuse of the musculo-skeletal system in space and on earth
M. Narici (2010)
10.1186/1471-2474-15-155
An integrated approach in a case of facioscapulohumeral dystrophy
S. Pasotti (2014)
Satellite cells in skeletal muscle atrophy and hypertrophy
T. Snijders (2014)
Effect of mechanical loading and ageing on myosin heavy chain turnover rate in fast-twitch skeletal muscle
A. Pehme (2004)
10.1201/B11928-10
Nitrogenous Compounds and Supplements
Jamie Landis (2012)
10.11606/T.42.2013.TDE-20092013-103029
EFEITO DA SUPLEMENTAÇÃO A CURTO PRAZO DE CREATINA SOBRE O EIXO GH-IGF-I, FUNÇÃO NEUROMUSCULAR E VIAS DE SÍNTESE E DEGRADAÇÃO PROTÉICA MUSCULAR EM RATOS
Lucas Guimarães Ferreira (2013)
immobilization and retraining Effects of aging on human skeletal muscle after
S. P. Magnusson (2015)
10.1152/JAPPLPHYSIOL.00167.2004
Body-weight-support treadmill training improves blood glucose regulation in persons with incomplete spinal cord injury.
Stuart M Phillips (2004)
10.1016/j.jnutbio.2009.07.007
Dietary fish oil inhibits the early stage of recovery of atrophied soleus muscle in rats via Akt-p70s6k signaling and PGF2α.
Jae-Sung You (2010)
10.1007/s12011-015-0253-3
Dose-dependent Effect of Boric Acid on Myogenic Differentiation of Human Adipose-derived Stem Cells (hADSCs)
Hüseyin Apdik (2015)
10.1007/978-981-13-1435-3_23
Nutritional Considerations in Preventing Muscle Atrophy.
S. Crețoiu (2018)
10.1016/J.LFS.2003.11.036
The effect of creatine supplementation upon inflammatory and muscle soreness markers after a 30km race.
R. T. Santos (2004)
Effects of chronic AICAR administration on fiber composition, glycolytic and oxidative enzyme activities and UCP3 and PGC-1 protein content in rat muscles
Masataka Suwa (2003)
LJMU Research Online Nutrition for the Prevention and Treatment of Injuries in Track and Field Athletes
Graeme L Close (2020)
10.1080/02640414.2011.587446
Dietary supplements for athletes: Emerging trends and recurring themes
R. Maughan (2011)
10.1016/J.CLNU.2004.03.004
Deleteriuos effects of immobilization upon rat skeletal muscle: role of creatine supplementation.
M. S. Aoki (2004)
10.1007/s00726-015-2161-4
New insights into the trophic and cytoprotective effects of creatine in in vitro and in vivo models of cell maturation
P. Sestili (2015)
10.1186/s12970-017-0173-z
International Society of Sports Nutrition position stand: safety and efficacy of creatine supplementation in exercise, sport, and medicine
R. Kreider (2017)
10.1016/j.arthro.2020.04.047
Supplement Use in Patients Undergoing Anterior Cruciate Ligament Reconstruction: A Systematic Review.
Dylan N. Greif (2020)
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