Online citations, reference lists, and bibliographies.
← Back to Search

In Vivo Response Of GsdmA3Dfl/+ Mice To Topically Applied Anti‐psoriatic Agents: Effects On Epidermal Thickness, As Determined By Optical Coherence Tomography And H&E Staining

M. H. Zulfakar, A. Alex, B. Povazay, W. Drexler, C. P. Thomas, R. Porter, C. Heard
Published 2011 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Abstract:  This study evaluated in vivo the potential of optical coherence tomography (OCT) to determine changes in thickness of the epidermis in response to the topically applied anti‐psoriatics betamethasone dipropionate (BD), salicylic acid (SA) and also fish oil (FO). GsdmA3Dfl/+ mice have an inflammatory hair loss phenotype that includes hyperproliferation and epidermal thickening, hence a potential psoriasis model. Changes in epidermal thickness were evaluated over a period of 10 days, with the mice treated with combined BD + SA, FO + SA and BD + FO + SA. The data were validated with conventional measurement using H&E staining coupled with microscopy. Initial baseline measurement revealed an average epidermal thickness of 26.92 ± 1.17 μm. After 10 days of treatment with BD, the average epidermal thickness was reduced by 38.8% (P = 0.0001), and inversely, treatment with FO resulted in an unexpected 105% increase (P = 0.0001) in epidermal thickness. Combined BD + FO treatment did not cause any significant change (P = 0.3755) and may further indicate opposing effects on keratinocyte proliferation. The data obtained using OCT were statistically the same as those obtained by H&E/microscopy (P = 0.4325), supporting a greater role for OCT in dermatological studies, while also allowing a reduction in the number of animals used in such studies as sacrifice at individual timepoints is not necessary.
This paper references
10.1016/J.DDMEC.2004.11.005
Mechanisms of psoriasis
F. Nestle (2004)
10.1007/S10043-009-0074-8
The characteristics of three-dimensional skin imaging system by full-colored optical coherence tomography
B. Yang (2009)
10.1016/J.CLINDERMATOL.2007.08.014
Animal models of psoriasis
W. Boehncke (2007)
10.1007/s00011-009-0065-z
Enhanced topical delivery and ex vivo anti-inflammatory activity from a betamethasone dipropionate formulation containing fish oil
M. H. Zulfakar (2009)
10.1016/j.preteyeres.2007.07.005
State-of-the-art retinal optical coherence tomography
W. Drexler (2008)
10.1111/j.1524-4725.2009.01164.x
Assessment of Optical Coherence Tomography Imaging in the Diagnosis of Non‐Melanoma Skin Cancer and Benign Lesions Versus Normal Skin: Observer‐Blinded Evaluation by Dermatologists and Pathologists
M. Mogensen (2009)
10.1016/S0377-1237(08)80108-6
Fitzpatrick's Dermatology in General Medicine
S. Grover (2008)
10.1001/ARCHDERM.135.9.1104
The immunologic and genetic basis of psoriasis.
B. Nickoloff (1999)
10.1016/j.cca.2008.04.005
Cytokines and anticytokines in psoriasis.
A. Pietrzak (2008)
10.1046/j.1365-4362.1999.00500.x
The role of salicylic acid in the treatment of psoriasis
M. Lebwohl (1999)
Drug Discovery Today: Disease
F ONestle (2004)
10.1354/vp.45-4-563
Review Paper: Preclinical Models of Psoriasis
D. Danilenko (2008)
10.1002/JPS.2600761004
Delivery of theophylline into excised human skin from alkanoic acid solutions: a "push-pull" mechanism.
R. Kadir (1987)
10.1007/s00403-009-1000-4
Optical coherence tomography imaging of psoriasis vulgaris: correlation with histology and disease severity
H. Morsy (2009)
10.1046/J.1523-1747.2002.01806.X
Defolliculated (dfl): a dominant mouse mutation leading to poor sebaceous gland differentiation and total elimination of pelage follicles.
R. Porter (2002)
10.1124/jpet.107.130955
7-Chloro-5-(4-hydroxyphenyl)-1-methyl-3-(naphthalen-2-ylmethyl)-4,5-dihydro-1H-benzo[b][1,4]diazepin-2(3H)-one (Bz-423), a Benzodiazepine, Suppresses Keratinocyte Proliferation and Has Antipsoriatic Activity in the Human Skin-Severe, Combined Immunodeficient Mouse Transplant Model
N. Bhagavathula (2008)
10.1088/0031-9155/52/5/N01
Validation of optical coherence tomography in vivo using cryostat histology.
T. Gambichler (2007)
10.1046/j.1365-2230.2002.01145_2.x
Fast Facts: Psoriasis
M. Vital (2002)
10.1111/1523-1747.EP12874482
Induction of epidermal hyperproliferation by topical n-3 polyunsaturated fatty acids on guinea pig skin linked to decreased levels of 13-hydroxyoctadecadienoic acid (13-hode).
C. C. Miller (1990)
10.1159/000118508
Morphology and Epidermal Thickness of Normal Skin Imaged by Optical Coherence Tomography
M. Mogensen (2008)
10.2174/157488507779422428
In vivo effects of topical anti-psoriatic treatments on cutaneous inflammation, epidermal proliferation and keratinisation.
P. Kerkhof (2007)
10.1016/J.IJPHARM.2006.05.018
Skin penetration enhancement of mefenamic acid by ethanol and 1,8-cineole can be explained by the 'pull' effect.
C. Heard (2006)
10.1016/S0140-6736(88)91181-6
A DOUBLE-BLIND, RANDOMISED, PLACEBO-CONTROLLED TRIAL OF FISH OIL IN PSORIASIS
S. B. Bittiner (1988)
10.1046/J.1523-1747.1999.00480.X
Evidence of nuclear PKC/MAP-kinase cascade in guinea pig model of epidermal hyperproliferation.
I. Mani (1999)
10.1001/archfaci.10.4.286
Fitzpatrick's Dermatology in General Medicine
Tobias Pincock (2003)
10.1117/1.3122373
Contrasting properties of gold nanoshells and titanium dioxide nanoparticles for optical coherence tomography imaging of skin: Monte Carlo simulations and in vivo study.
M. Kirillin (2009)
10.1016/J.PLEFA.2007.05.002
Probing the skin permeation of fish oil/EPA and ketoprofen-3. Effects on epidermal COX-2 and LOX.
C. P. Thomas (2007)
Epub ahead of print
M Mogensen (2009)
10.1079/BJN2001459
n-3 fatty acids in psoriasis.
P. Mayser (2002)
10.1038/SJ.JID.5700807
Mouse models of psoriasis.
J. Gudjonsson (2007)
Optical Coherence Tomography of Ocular Disease
J Schuman (2004)
10.1016/J.PLEFA.2007.10.015
Immunomodulation by omega-3 fatty acids.
P. Calder (2007)
10.1038/nature03963
Psoriasis-like skin disease and arthritis caused by inducible epidermal deletion of Jun proteins
R. Zenz (2005)
10.1046/J.1523-1747.1999.00538.X
Animal models of psoriasis - what can we learn from them?
M. Schön (1999)
10.1016/J.IJPHARM.2005.06.013
Preferential π–π complexation between tamoxifen and borage oil/γ linolenic acid: transcutaneous delivery and NMR spectral modulation
C. Heard (2005)
10.1093/JN/117.8.1360
Dietary influences of evening primrose and fish oil on the skin of essential fatty acid-deficient guinea pigs.
R. Chapkin (1987)
10.1111/j.1365-2133.2006.07369.x
Evaluation of the atrophogenic potential of different glucocorticoids using optical coherence tomography, 20‐MHz ultrasound and profilometry; a double‐blind, placebo‐controlled trial
M. Cossmann (2006)
Optical Coherence Tomography of Ocular Diseases
J. Schuman (1995)
10.1111/J.0022-202X.2005.23623.X
Mutations in gasdermin 3 cause aberrant differentiation of the hair follicle and sebaceous gland.
D. Lunny (2005)
10.1088/0034-4885/66/2/204
Optical coherence tomography - principles and applications
A. Fercher (2003)



This paper is referenced by
10.1111/exd.12196
Optical coherence tomography for presurgical margin assessment of non‐melanoma skin cancer – a practical approach
Seyed Arash Alawi (2013)
10.1007/s00403-014-1498-y
Optical coherence tomography in the diagnosis of basal cell carcinoma
A. Hussain (2014)
10.1002/jbio.201200069
Evaluation of zebrafish brain development using optical coherence tomography.
Yu-Sheng Lin (2013)
10.1007/978-1-4614-5034-4_14
Diagnosis and the Skin Immune System
A. Nasir (2013)
10.1159/000366543
Optical coherence tomography and its role for delineating the thickness of keratinocyte dysplasia and neoplasia.
L. Themstrup (2015)
10.1007/s00418-017-1542-z
Gsdma3 is required for mammary gland development in mice
Haiying Guo (2017)
10.1111/jdv.12935
Assessment of a scoring system for Basal Cell Carcinoma with multi‐beam optical coherence tomography
C. Wahrlich (2015)
10.1002/2211-5463.12095
In vivo response of GsdmA3Dfl/+ mice to topically applied fish oil – effects on cellular markers and macrophages
M. H. Zulfakar (2016)
10.1042/BJ20150204
Loss of conserved Gsdma3 self-regulation causes autophagy and cell death.
P. Shi (2015)
Imaging elastographic contrast in optical coherence tomographyfor applications in dermatology and oncology
A. Grimwood (2012)
10.1111/j.1600-0625.2012.01569.x
High‐definition optical coherence tomography enables visualization of individual cells in healthy skin: comparison to reflectance confocal microscopy
M. Boone (2012)
10.1016/j.jdermsci.2012.05.001
Gsdma3(I359N) is a novel ENU-induced mutant mouse line for studying the function of Gasdermin A3 in the hair follicle and epidermis.
S. Kumar (2012)
10.1016/j.jprot.2013.09.010
A proteomics approach to the identification of biomarkers for psoriasis utilising keratome biopsy.
J. Williamson (2013)
10.1111/j.1600-0846.2011.00558.x
Using optical coherence tomography for the longitudinal non‐invasive evaluation of epidermal thickness in a murine model of chronic skin inflammation
Rachel J. Silver (2012)
Semantic Scholar Logo Some data provided by SemanticScholar