Online citations, reference lists, and bibliographies.

Isolation Of Campylobacter From Feral Swine (Sus Scrofa) On The Ranch Associated With The 2006 Escherichia Coli O157:H7 Spinach Outbreak Investigation In California.

Michele T Jay-Russell, A Bates, L Harden, William G. Miller, Robert E. Mandrell
Published 2012 · Medicine, Biology
Cite This
Download PDF
Analyze on Scholarcy
We report the isolation of Campylobacter species from the same population of feral swine that was investigated in San Benito County, California, during the 2006 spinach-related Escherichia coli O157:H7 outbreak. This is the first survey of Campylobacter in a free-ranging feral swine population in the United States. Campylobacter species were cultured from buccal and rectal-anal swabs, colonic faeces and tonsils using a combination of selective enrichment and antibiotic-free membrane filtration methods. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS, Bruker Daltonics, Inc., Billerica, MA, USA) was used to identify species followed by confirmatory multiplex PCR or 16S rRNA sequencing. Genetic relatedness of Campylobacter jejuni and Campylobacter coli strains was determined by multilocus sequence typing (MLST) and porA allele sequencing. Altogether, 12 (40%) of 30 feral swine gastrointestinal and oral cavity specimens were positive, and six species were isolated: Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalsis, Campylobacter jejuni, Campylobacter lanienae and Campylobacter sputorum. Campylobacter jejuni subtypes were closely related to MLST sequence type 21 (ST-21) and had identical porA sequences. Campylobacter coli subtypes were unrelated to isolates in the pubMLST/porA database. This feral swine population lived in close association with a 'grassfed' beef cattle herd adjacent to spinach and other leafy green row crop fields. The findings underscore the importance of protecting raw vegetable crops from faecal contamination by wild or feral animals. The study also illustrates a potential risk of Campylobacter exposure for hunters during handling and processing of wild swine meat.
This paper references
Evaluation of Preston medium for detection ofCampylobacter jejuni in vitroand in artificially and naturally contaminated poultry products
Mieke Uyttendaele (1996)
Brucella suis infection associated with feral swine hunting - three states, 2007-2008.
Dana Giurgiutiu (2009)
Survey of Campylobacter species, VTEC 01 57 and Salmonella species in Swedish wildlife
Helene Wahlstroem (2003)
Estimating sizes of wild pig populations in the north and central coast regions of California
Richard Alan Sweitzer (2000)
Campylobacter Species and Fresh Produce: Outbreaks, Incidence, and Biology
Robert E. Mandrell (2008)
Speciation of Campylobacter coli, C. jejuni, C. helveticus, C. lari, C. sputorum, and C. upsaliensis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Robert E. Mandrell (2005)
Campylobacter bacteremia: clinical features and factors associated with fatal outcome.
Jérôme Pacanowski (2008)
Campylobacter jejuni—An Emerging Foodborne Pathogen
Sean F Altekruse (1999)
Molecular characterization of Campylobacter lanienae strains isolated from food-producing animals.
Nóra Schweitzer (2011)
Survival of Campylobacter jejuni on various fresh produce.
Rauni Kärenlampi (2004)
Transmission of Campylobacter hyointestinalis from a Pig to a Human
Gregor Gorkiewicz (2002)
An epidemic of trichinosis after ingestion of wild pig in Hawaii.
Elizabeth Barrett-connor (1976)
Extended Multilocus Sequence Typing System for Campylobacter coli, C. lari, C. upsaliensis, and C. helveticus
William G. Miller (2005)
Feral hogs (Sus scrofa)
J. R. Sweeney (2003)
Trends of brucellosis in Florida. An epidemiologic review.
W. B. Bigler (1977)
Campylobacter lanienae sp. nov., a new species isolated from workers in an abattoir.
Julie M J Logan (2000)
Escherichia coli O157:H7 in Feral Swine near Spinach Fields and Cattle, Central California Coast1
Michele T. Jay (2007)
Genetic detection of Campylobacter lanienae in fecal matter and stored manure from swine and dairy cattle.
Evelyne Guévremont (2008)
The Cape Town protocol: how to isolate the most Campylobacter for your dollar, pound, franc
E. Le Roux (1998)
Colonization of Cattle Intestines by Campylobacter jejuni and Campylobacter lanienae
G. Douglas Inglis (2005)
Prevalence and diversity of Campylobacter jejuni in pig herds on farms with and without cattle or poultry.
Jaap Boes (2005)
Transmission and Infectious Dose of Escherichia coli O157:H7 in Swine
Nancy A. Cornick (2004)
Disease Risks Associated with Increasing Feral Swine Numbers and Distribution in the United States
Thomas Hutton (2006)
Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA
John David Klena (2004)
Campylobacter upsaliensis, C. sputorum sputorum and C. concisus as common causes of diarrhoea in Swedish children.
Gerhard Lindblom (1995)
Outbreak of campylobacteriosis associated with consumption of raw peas.
Tracie J. Gardner (2011)
Extended Sequence Typing of Campylobacter spp., United Kingdom
Kate E. Dingle (2008)
Comparison of Survival of Campylobacter jejuni in the Phyllosphere with That in the Rhizosphere of Spinach and Radish Plants
Maria Theresa Brandl (2004)
Prevalence of and associated risk factors for shedding Cryptosporidium parvum oocysts and Giardia cysts within feral pig populations in California.
Edward R Atwill (1997)
Food Safety Risks and Mitigation Strategies for Feral Swine (Sus scrofa) near Agriculture Fields
Michele T. Jay (2008)

This paper is referenced by
Natural Resources California Agriculture Title Wild pigs breach farm fence through harvest time in southern San Joaquin Valley Permalink
Michael D. White (2018)
Enteric Human Pathogens of Wild Boar, Feral Swine, and Javelina (Order: Artiodactyla)
Michele T Jay-Russell (2014)
There's a frog in my salad! A review of online media coverage for wild vertebrates found in prepackaged produce in the United States.
Daniel F. Hughes (2019)
Chapter 12 - Food Pathogens
Işın Akyar (2015)
Campylobacter Shared Between Free-Ranging Cattle and Sympatric Wild Ungulates in a Natural Environment (NE Spain)
Nora Navarro-Gonzalez (2014)
Comparative Genomic Analysis Identifies a Campylobacter Clade Deficient in Selenium Metabolism
William G. Miller (2017)
A Review of the Current Status of Relevant Zoonotic Pathogens in Wild Swine (Sus scrofa) Populations: Changes Modulating the Risk of Transmission to Humans
Francisco Ruiz-Fons (2017)
Campylobacter infection in wild artiodactyl species from southern Spain: occurrence, risk factors and antimicrobial susceptibility.
A. Carbonero (2014)
PorA specific primers for the identification of Campylobacter species in food and clinical samples
Marco Fontanot (2014)
Wild pigs breach farm fence through harvest time in southern San Joaquin Valley
Michael D. White (2018)
Food Safety Risks from Wildlife
Michele T Jay-Russell (2016)
Overview: Foodborne Pathogens in Wildlife Populations
Marilyn C Erickson (2016)
Reducing Foodborne Pathogen Persistence and Transmission in Animal Production Environments: Challenges and Opportunities.
Elaine D. Berry (2016)
Enteropathogenic Yersinia spp. and Salmonella spp. in Swedish wild boars
Axel Sannö (2018)
Pathogenomics of Emerging Campylobacter Species
Daniela Costa (2019)
Fecal shedding of Campylobacter jejuni and Campylobacter coli among feral pigs in Texas
Kevin J Cummings (2018)
Carriage and Subtypes of Foodborne Pathogens Identified in Wild Birds Residing near Agricultural Lands in California: a Repeated Cross-Sectional Study
Nora Navarro-Gonzalez (2019)
Governmental Provisions to Manage and Eradicate Feral Swine in Areas of the United States
Terence J. Centner (2014)
First reported case of Campylobacter lanienae enteritis in a human
Simon Lévesque (2016)
What is the risk from wild animals in food-borne pathogen contamination of plants?
Michele T Jay-Russell (2013)
Prevalence and risk factors associated with Campylobacter spp. and Salmonella enterica in livestock raised on diversified small-scale farms in California
Alda F A Pires (2019)
Cross-species transmission potential between wild pigs, livestock, poultry, wildlife, and humans: implications for disease risk management in North America
Ryan S. Miller (2017)
Potential role of wildlife in pathogenic contamination of fresh produce
Jeff Langholz (2013)
Prevalence and antimicrobial susceptibility of foodborne bacteria in wild boars (Sus scrofa) and wild deer (Cervus nippon) in Japan.
Yoshimasa Sasaki (2013)
Comparative Genomics of All Three Campylobacter sputorum Biovars and a Novel Cattle-Associated C. sputorum Clade
William G. Miller (2017)
Species distribution, genetic diversity and antimicrobial susceptibility of Campylobacter isolates recovered from the preputial cavity of healthy rams in Turkey.
Fuat Aydın (2020)
Molecular typing and source attribution of Finnish Campylobacter jejuni isolates
Astrid de Haan (2012)
Semantic Scholar Logo Some data provided by SemanticScholar