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Use Of Steady-state Fluorescence Anisotropy With Pebble Nanosensors For Chemical Analysis

T. Horvath, E. Monson, J. S. Sumner, H. Xu, R. Kopelman
Published 2002 · Chemistry, Engineering

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In this study we show that steady-state fluorescence anisotorpy within PEBBLEs can be used for the optochemical sensing of analytes such as Zn2+, O2, and Ca2+. Steady-state fluorescence anisotropy is a non- time resolved method that measures a combination of rotational and fluorescence lifetimes. This eliminates the need for reference dyes and ratiometic techniques to obtain quantitative results, even when using intensity-based sensor dyes. An advantage to working with PEBBLE nanosensors is that the encapsulated dye is localized in a constant rotational environment. This is in contrast to the use of free dyes, which can be affected by interferents such as protein binding.
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