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Multifunctional Encoded Particles For High-Throughput Biomolecule Analysis

D. Pregibon, M. Toner, P. Doyle
Published 2007 · Medicine

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High-throughput screening for genetic analysis, combinatorial chemistry, and clinical diagnostics benefits from multiplexing, which allows for the simultaneous assay of several analytes but necessitates an encoding scheme for molecular identification. Current approaches for multiplexed analysis involve complicated or expensive processes for encoding, functionalizing, or decoding active substrates (particles or surfaces) and often yield a very limited number of analyte-specific codes. We present a method based on continuous-flow lithography that combines particle synthesis and encoding and probe incorporation into a single process to generate multifunctional particles bearing over a million unique codes. By using such particles, we demonstrate a multiplexed, single-fluorescence detection of DNA oligomers with encoded particle libraries that can be scanned rapidly in a flow-through microfluidic channel. Furthermore, we demonstrate with high specificity the same multiplexed detection using individual multiprobe particles.
This paper references
10.1038/90228
Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules
M. Han (2001)
Nano Lett
X Su (2005)
10.1021/JA00148A039
Radio Frequency Tag Encoded Combinatorial Library Method for the Discovery of Tripeptide-Substituted Cinnamic Acid Inhibitors of the Protein Tyrosine Phosphatase PTP1B
E. Moran (1995)
Angew. Chem. Int. Ed
R Otero (2004)
10.1021/LA0534625
Magnetically and biologically active bead-patterned hydrogels.
D. Pregibon (2006)
Prog. Surf. Sci
F Rosei (2003)
Langmuir
D C Pregibon (2006)
Anal. Chem
N H Finkel (2004)
Acc. Chem. Res
J S Moore (1997)
10.1038/364555A0
Multiplexed biochemical assays with biological chips
S. P. Fodor (1993)
J. Am. Chem. Soc
G S Mccarty (2004)
10.1021/JA035665Q
Preparation, physical properties, on-bead binding assay and spectroscopic reliability of 25 barcoded polystyrene-poly(ethylene glycol) graft copolymers.
H. Fenniri (2003)
10.1021/AC034165C
Micromachining microcarrier-based biomolecular encoding for miniaturized and multiplexed immunoassay.
Zheng-liang Zhi (2003)
Phys. Rev. Lett
L Gross (2004)
Phys. Rev. Lett
S Lukas (2002)
10.1089/154065803321537926
An encoded particle array tool for multiplex bioassays.
M. Evans (2003)
10.1093/NAR/27.2.649
Immobilization of acrylamide-modified oligonucleotides by co-polymerization.
F. Rehman (1999)
10.1038/NMAT1617
Continuous-flow lithography for high-throughput microparticle synthesis
D. Dendukuri (2006)
J. Am. Chem. Soc
B J Battersby (2000)
Nat. Mater
R Otero (2004)
Phys. Rev. B
J Perdew (1992)
10.1128/JCM.26.10.1957-1961.1988
Simultaneous detection of antibodies to cytomegalovirus and herpes simplex virus by using flow cytometry and a microsphere-based fluorescence immunoassay.
T. McHugh (1988)
Phys. Rev. B
D Vanderbilt (1990)
10.1016/S0167-7799(01)01844-3
Suspension array technology: evolution of the flat-array paradigm.
J. P. Nolan (2002)
A corresponding movie appears on Science Online
10.1038/416885A
Microarray technology: An array of opportunities
D. Gershon (2002)
10.1021/NL0484088
Composite organic-inorganic nanoparticles (COINs) with chemically encoded optical signatures.
Xing Su (2005)
Acc. Chem. Res
E C H Sykes (2003)
10.1038/NMAT702
Biomolecular screening with encoded porous-silicon photonic crystals
F. Cunin (2002)
10.1021/AC0008284
High-density fiber-optic DNA random microsphere array.
J. A. Ferguson (2000)
10.1021/CM060283F
Uniformly Colorized Beads for Multiplex Immunoassay
Xiangwei Zhao (2006)
Biotechniques
A Y Rubina (2003)
Biotechniques
A V Vasiliskov (1999)
Anal. Chem
J A Ferguson (2000)
Acc. Chem. Res
J P Collin (2001)
10.1093/NAR/GNG043
Multiplexed SNP genotyping using the Qbead system: a quantum dot-encoded microsphere-based assay.
H. Xu (2003)
10.1093/CLINCHEM/43.9.1749
Advanced multiplexed analysis with the FlowMetrix system.
R. J. Fulton (1997)
Nat. Rev. Drug Discov
K Braeckmans (2002)
Phys. Rev. Lett
J Repp (2000)
10.1109/20.718537
Physical limits of hyperthermia using magnetite fine particles
R. Hergt (1998)
J. Am. Chem. Soc
H Fenniri (2003)
10.1002/ANIE.199522891
Radiofrequency Encoded Combinatorial Chemistry
K. C. Nicolaou (1995)
10.1126/SCIENCE.294.5540.137
Submicrometer metallic barcodes.
S. R. Nicewarner-Peña (2001)
Rev. Mod. Phys
M Payne (1992)
10.1007/S00216-005-0225-0
Multiplexed SNP genotyping using nanobarcode particle technology
M. Sha (2006)
Assay Drug Dev. Technol
M Evans (2003)
10.1038/nrd817
Encoding microcarriers: present and future technologies
K. Braeckmans (2002)
Phys. Rev. B
G Kresse (1993)
Department of Energy joint grants DE-FGO2-03ER15464/5
Angew. Chem. Int. Ed
K C Nicolaou (1995)
Nat. Mater
D Dendukuri (2006)
10.2144/03345RR01
Hydrogel-based protein microchips: manufacturing, properties, and applications.
A. Rubina (2003)
Phys. Chem. Chem. Phys
K H Ernst (1999)
10.1021/JA993634I
Toward larger chemical libraries: Encoding with fluorescent colloids in combinatorial chemistry
B. Battersby (2000)
Nat. Mater
K Braeckmans (2003)
Phys. Rev. Lett
K Y Kwon (2005)
Chem. Mater
X W Zhao (2006)
Chem. Rev
G S Kottas (2005)
Anal. Chem
Z L Zhi (2003)
疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
宁北芳 (2005)
Nucleic Acids Res
F N Rehman (1999)
Nat. Biotechnol
M Han (2001)
Anal. Bioanal. Chem
M Y Sha (2006)
Nat. Rev. Genet
J B Fan (2006)
To whom correspondence should be addressed
J. Vivanco (2009)
10.1038/nrg1901
Highly parallel genomic assays
J. Fan (2006)
Angew. Chem. Int. Ed
V Balzani (2000)
We gratefully acknowledge support from NSF grant CTS-0304128 and the Dumbros Fellowship
10.2144/99273RR06
Fabrication of microarray of gel-immobilized compounds on a chip by copolymerization.
A. V. Vasiliskov (1999)
10.1021/AC0416463
Barcoding the microworld.
Nancy H. Finkel (2004)
Phys. Rev. Lett
T Mitsui (2005)
Department of Energy joint grants DE-FGO2-03ER15464/5. We thank the Academic Associates Program of the San Diego Supercomputer Center for computational resources
J. Clin. Microbiol
T M Mchugh (1988)
10.1073/PNAS.97.14.7692
Euclidean shape-encoded combinatorial chemical libraries.
A. R. Vaino (2000)
Trends Biotechnol
J P Nolan (2002)
J. R. Kettman Jr. Clin. Chem
R J Fulton (1997)
Nat. Mater
F Cunin (2002)
10.1038/NMAT828
Encoding microcarriers by spatial selective photobleaching
K. Braeckmans (2003)
Nucleic Acids Res
H Xu (2003)
J. Am. Chem. Soc
E J Moran (1995)
10.1126/SCIENCE.289.5485.1760
Printing proteins as microarrays for high-throughput function determination.
G. MacBeath (2000)
10.1016/j.otohns.2009.05.016
Nature
R. Rosenfeld (2009)
IEEE Trans. Magn
R Hergt (1998)
Materials and methods are availabe as supporting material on Science Online



This paper is referenced by
A UNIVERSAL PARTICLE ENCODING ARCHITECTURE
Jongbae Lee (2013)
Novel planar and particle-based microarrays for point-of-care diagnostics
Gustav Svedberg (2019)
Development of Microcontact Printing Techniques for Fabricating Functional Micropatterns and Microparticles for Biomedical Applications
Z. Wang (2015)
10.3929/ethz-a-007303687
Complex particle assemblies on surfaces
Cyrill Kümin (2011)
10.1039/C6TB00476H
C8-modified CeO2//SiO2 Janus fibers for selective capture and individual MS detection of low-abundance peptides and phosphopeptides.
N. Lv (2016)
10.1002/chem.201002585
Aptamer/nanoparticle-based sensitive, multiplexed electronic coding of proteins and small biomolecules through a backfilling strategy.
Xiaoqing Qian (2010)
10.1002/smll.201701625
Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning.
X. Qiu (2017)
10.1007/S10404-008-0303-7
Development of microfluidic devices incorporating non-spherical hydrogel microparticles for protein-based bioassay
D. Choi (2008)
10.1246/CL.130625
Fabrication of Cellulose-based Microparticles at Gas–Liquid Interfaces in a Microchannel
Na Rea Lee (2013)
10.1039/c5cc02048d
Shape-encoded silica microparticles for multiplexed bioassays.
Lily Nari Kim (2015)
10.1007/S10404-007-0240-X
Microfabricated micropallets for enhancement of biomolecular techniques
C. Jensen-McMullin (2008)
10.1002/ADMA.200900223
Production of Quantum Dot Barcodes Using Biological Self‐Assembly
Sakandar Rauf (2009)
10.1021/nn800332g
Smart nanomaterials.
M. Yoshida (2008)
10.1007/s00604-014-1217-6
Fluorescence energy transfer-based multiplexed hybridization assay using gold nanoparticles and quantum dot conjugates on photonic crystal beads
J. Li (2014)
unrepeatable and still better person Janus particles for ( bio ) sensing
P. Yánez-Sedeñoa (2017)
10.1038/srep22975
Extensible Multiplex Real-time PCR of MicroRNA Using Microparticles
S. Jung (2016)
10.1007/s10544-013-9752-1
Shadow masking for nanomaterial-based biosensors incorporated with a microfluidic device
J. Huang (2013)
10.1039/c0lc00342e
Multiplexed detection of nucleic acids in a combinatorial screening chip.
B. Schudel (2011)
10.1002/asia.201601259
Azo-Polymer Janus Particles Assembled by Solvent-Induced Microphase Separation and Their Photoresponsive Behavior.
Xinran Zhou (2016)
10.1016/j.talanta.2013.12.060
Microfluidic fabrication of multifunctional particles and their analytical applications.
Xiao-ting Sun (2014)
10.1002/elps.201900322
Complex three‐dimensional microparticles from microfluidic lithography
Ye Tian (2020)
10.1039/c6an00188b
Vertically encoded tetragonal hydrogel microparticles for multiplexed detection of miRNAs associated with Alzheimer's disease.
Y. H. Roh (2016)
Modeling particle suspensions using lattice Boltzmann method
W. Mao (2013)
10.1039/b821930c
Lock release lithography for 3D and composite microparticles.
Ki Wan Bong (2009)
10.1016/j.addr.2013.07.004
Microfluidics-assisted in vitro drug screening and carrier production.
J. Tsui (2013)
Programming DNA for molecular-scale temporal barcoding and enzymatic computation
Shalin Shah (2020)
10.1038/nrd3799
Revisiting lab-on-a-chip technology for drug discovery
P. Neužil (2012)
10.1002/ADMA.200702622
Microspheres with Tunable Refractive Index by Controlled Assembly of Nanoparticles
S. Kim (2008)
10.1002/9780470431818.CH2
High‐Throughput Protein‐Based Technologies and Computational Models for Drug Development, Efficacy, and Toxicity
L. Alexopoulos (2008)
10.1002/advs.201802342
Gas‐Shearing Fabrication of Multicompartmental Microspheres: A One‐Step and Oil‐Free Approach
Guosheng Tang (2019)
10.1021/acs.analchem.6b01475
Manipulation of a Single Circulating Tumor Cell Using Visualization of Hydrogel Encapsulation toward Single-Cell Whole-Genome Amplification.
T. Yoshino (2016)
10.1039/c4sc02696a
Multiplexed DNA detection based on positional encoding/decoding with self-assembled DNA nanostructures† †Electronic supplementary information (ESI) available: Experimental details, additional DNA detection figures, and DNA sequence information. See DOI: 10.1039/c4sc02696a Click here for additional d
Sha Sun (2015)
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