Online citations, reference lists, and bibliographies.
← Back to Search

Harpin, Elicitor Of The Hypersensitive Response Produced By The Plant Pathogen Erwinia Amylovora.

Z. Wei, R. J. Laby, C. H. Zumoff, D. W. Bauer, S. He, A. Collmer, S. V. Beer
Published 1992 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
A proteinaceous elicitor of the plant defense reaction known as the hypersensitive response was isolated from Erwinia amylovora, the bacterium that causes fire blight of pear, apple, and other rosaceous plants. The elicitor, named harpin, is an acidic, heat-stable, cell-envelope-associated protein with an apparent molecular weight of 44 kilodaltons. Harpin caused tobacco leaf lamina to collapse and caused an increase in the pH of bathing solutions of suspension-cultured tobacco cells. The gene encoding harpin (hrpN) was located in the 40-kilobase hrp gene cluster of E. amylovora, sequenced, and mutated with Tn5tac1. The hrpN mutants were not pathogenic to pear, did not elicit the hypersensitive response, and did not produce harpin.
This paper references
Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity.
T. V. Huynh (1989)
Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations.
W. Y. Chow (1988)
Molecular Genetics of Pathogenicity in Phytopathogenic Bacteria
M. Daniels (1988)
A genetic approach to analyzing membrane protein topology.
C. Manoil (1986)
Molecular cloning of a Pseudomonas syringae pv. syringae gene cluster that enables Pseudomonas fluorescens to elicit the hypersensitive response in tobacco plants.
H. C. Huang (1988)
Expression of Erwinia amylovora hrp genes in response to environmental stimuli.
Z. Wei (1992)
Rapid Detection of the Pathogenicity of Phytopathogenic Pseudomonads
Z. Klement (1963)
The Quantitative Relation Between Plant and Bacterial Cells Involved in the Hypersensitive Reaction
J. G. Turner (1974)
Improved tools for biological sequence comparison.
W. Pearson (1988)
Thyroid Gland and Temperature Tolerance Relationships in Cold-blooded Vertebrates
J. Dodd (1963)
New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.
J. Way (1984)
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
S. Tabor (1985)
Concurrent loss in Tn5 mutants of Pseudomonas syringae pv. syringae of the ability to induce the hypersensitive response and host plasma membrane K+/H+ exchange in tobacco
C. J. Baker (1987)
The Hypersensitive Reaction of Tobacco to Pseudomonas syringae pv. pisi: Activation of a Plasmalemma K/H Exchange Mechanism.
M. Atkinson (1985)

This paper is referenced by
A pleiotropic reduced virulence (Rvi‐) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens
V. Mulholland (1993)
Proteinaceous Elicitors of Plant Defense Responses
P. Ricci (1993)
Similarity between the Rhizobium meliloti fliP gene and pathogenicity-associated genes from animal and plant pathogens.
T. Finan (1995)
Cloning of genes required for hypersensitivity and pathogenicity inPseudomonas syringae pv.aptata
P. Minardi (2004)
Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase‐like enzyme
R. Desikan (1996)
Biotechnology in Plant Protection
F. Niepold (2001)
Identification of regulators of hrp/hop genes of Erwinia carotovora ssp. carotovora and characterization of HrpL(Ecc) (SigmaL(EccM)), an alternative sigma factor.
A. Chatterjee (2002)
Growth promotion may compensate for losses due to moderate Aphanomyces root rot.
Michael S. Metzger (2008)
Nonidet p-40, a novel inducer, activates cucumber disease resistance against cucumber anthracnose disease
Ta Chen Lin (2014)
Exploitation of Induced Resistance: A Commercial Perspective
A. Leadbeater (2014)
Transcriptome analysis of Acidovorax avenae subsp. avenae cultivated in vivo and co-culture with Burkholderia seminalis
B. Li (2014)
Negative regulation of hrp genes in Pseudomonas syringae by HrpV.
G. Preston (1998)
Amplification, Sequence and Expression of the Harpin Encoding Gene of the Bacterial Canker Pathogen, Pseudomonas syringae pv. syringae Strain NV
M. Appel (1999)
Type III secretion machines: bacterial devices for protein delivery into host cells.
J. Galán (1999)
Signal Transduction in Host-Parasite Interactions
J. Huang (2001)
Characterization of the hrp pathogenicity cluster of Erwinia carotovora subsp. carotovora: high basal level expression in a mutant is associated with reduced virulence
S. Lehtimäki (2003)
Fold modulating function: bacterial toxins to functional amyloids
Adnan K. Syed (2014)
Genetic transfer of amylovoran and stewartan synthesis between Erwinia amylovora and Erwinia stewartii.
F. Bernhard (1996)
The pathosystem Erwinia amylovora – Pyrus communis: A multidisciplinary study of possible plant defence mechanisms with focus on the phenylpropanoid-flavonoid pathway
K. Vrancken (2013)
A pleiotropic reduced virulence (Rvi−) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens
V. Mulholland (1993)
The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69
S. J. Elliott (1998)
Metabolic engineering of bioactive compounds in berries
Ivayla Dincheva (2015)
Molecular signature of differential virulence in natural isolates of Erwinia amylovora.
Dongping Wang (2010)
COMMENTARY Why do pathogens carry avirulence genes
D. Gabriel (1999)
Prevention of Copper-Induced Calcium Influx and Cell Death by Prion-Derived Peptide in Suspension-Cultured Tobacco Cells
T. Kagenishi (2009)
Tissue Induced during Infection of Immature Pear Genes Erwinia amylovora Identification of
Youfu Zhao (2005)
Diversifying microbial natural products for drug discovery
V. Knight (2003)
Applicationof signature-taggedmutagenesis to the studyof virulenceofErwinia amylovora
Limei Wang (2006)
Indução de resistência em plantas de berinjela e tomate por Lentinula edodes e Agaricus blazei contra bactérias causadoras de murcha (Ralstonia solanacearum) e cancro (Clavibacter michiganensis subsp. michiganensis)
R. F. Silva (2007)
Evidence that the Pseudomonas syringae pv. syringae hrp-linked hrmA gene encodes an Avr-like protein that acts in an hrp-dependent manner within tobacco cells.
J. Alfano (1997)
Concepts actuels sur la pathogénie chez les bactéries phytopathogènes
C. Boucher (2001)
Purification and immunocytolocalization of a novel Phytophthora boehmeriae protein inducing the hypersensitive response and systemic acquired resistance in tobacco and Chinese cabbage
Y. Wang (2003)
See more
Semantic Scholar Logo Some data provided by SemanticScholar