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Lymphocyte Apoptosis: Mediation By Increased Type 3 Inositol 1,4,5-Trisphosphate Receptor

A. Khan, M. Soloski, A. Sharp, G. Schilling, D. Sabatini, S. Li, C. Ross, S. Snyder
Published 1996 · Biology, Medicine

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B and T lymphocytes undergoing apoptosis in response to anti-immunoglobulin M antibodies and dexamethasone, respectively, were found to have increased amounts of messenger RNA for the inositol 1,4,5-trisphosphate receptor (IP3R) and increased amounts of IP3R protein. Immunohistochemical analysis revealed that the augmented receptor population was localized to the plasma membrane. Type 3 IP3R (IP3R3) was selectively increased during apoptosis, with no enhancement of type 1 IP3R (IP3R1). Expression of IP3R3 antisense constructs in S49 T cells blocked dexamethasone-induced apoptosis, whereas IP3R3 sense, IP3R1 sense, or IP3R1 antisense control constructs did not block cell death. Thus, the increases in IP3R3 may be causally related to apoptosis.
This paper references
Characterization of apoptosis in thymocytes isolated from dexamethasone-treated rats.
X. Sun (1992)
Characterization of inositol 1,4,5-trisphosphate receptors and calcium mobilization in a hepatic plasma membrane fraction.
G. Guillemette (1988)
Similar actions of glucocorticoids and calcium on the regulation of apoptosis in S49 cells.
L. A. Caron-Leslie (1991)
Plasma membrane inositol 1,4,5-trisphosphate receptor of lymphocytes: selective enrichment in sialic acid and unique binding specificity.
A. Khan (1992)
Early signal transduction by the antigen receptor without commitment to T cell activation.
M. Goldsmith (1988)
DNA fragmentation in S49 lymphoma cells killed with glucocorticoids and other agents
W. Vedeckis (1983)
Programmed death of T cells in HIV-1 infection.
L. Meyaard (1992)
Localization of inositol 1,4,5-trisphosphate receptor-like protein in plasmalemmal caveolae
T. Fujimoto (1992)
Differentiated functions expressed by cultured mouse lymphoma cells. I. Specificity and kinetics of cell responses to corticosteroids.
A. W. Harris (1970)
The role of the T3/antigen receptor complex in T-cell activation.
A. Weiss (1986)
Neuronal inositol 1,4,5-trisphosphate receptor localized to the plasma membrane of olfactory cilia
A. Cunningham (1993)
Role of T3 surface molecules in human T-cell activation: T3-dependent activation results in an increase in cytoplasmic free calcium.
A. Weiss (1984)
IP3 receptor purified from liver plasma membrane is an (1,4,5)IP3 activated and (1,3,4,5)IP4 inhibited calcium permeable ion channel.
M. Mayrleitner (1995)
Different localization of inositol 1,4,5-trisphosphate and ryanodine binding sites in rat liver.
L. Feng (1992)
Glucocorticoids activate a suicide process in thymocytes through an elevation of cytosolic Ca2+ concentration.
D. McConkey (1989)
Inositol phosphates and cell signalling
M. Berridge (1989)
Plasma membrane inositol 1,4,5-Trisphosphate-Activated channels mediate signal transduction in lobster olfactory receptor neurons
D. A. Fadool (1992)
Features of apoptotic cells measured by flow cytometry.
Z. Darzynkiewicz (1992)
Inositol 1,4,5-trisphosphate receptors. Localization in epithelial tissue.
A. Sharp (1992)
Potassium and calcium channels in lymphocytes.
R. S. Lewis (1995)
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
Calcium-mediated mechanisms in chemically induced cell death.
P. Nicotera (1992)
Patch clamp studies of lymphocyte activation.
P. Gardner (1990)
Keck Foundation and by USPHS grants Al-20922
IP3 receptor: localization to plasma membrane of T cells and cocapping with the T cell receptor.
A. Khan (1992)
Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons
C. Ross (1989)
The regulation of growth and differentiation of a murine B cell lymphoma. II. The inhibition of WEHI 231 by anti-immunoglobulin antibodies.
A. Boyd (1981)
Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death.
J. Cohen (1984)
Antibodies to synthetic IP3R peptides
Harlow (1988)
The inositol 1,4,5-trisphosphate receptor is localized on specialized sub-regions of the endoplasmic reticulum in rat liver.
J. Lièvremont (1994)
The polymerase chain reaction (PCR) was used to create DNA fragments corresponding to positions -81 to +9 of IP3R1 and lP3R3 mRNA
Cellular signalling in programmed cell death (apoptosis).
D. McConkey (1990)
Regulation of calcium influx by second messengers in rat mast cells
R. Penner (1988)
Calcium dependence of glucocorticoid-induced lymphocytolysis.
N. Kaiser (1977)
T-cell apoptosis detected in situ during positive and negative selection in the thymus
C. Surh (1994)
Anti‐immunoglobulin antibodies induce apoptosis in immature B cell lymphomas
J. Hasbold (1990)
Total RNA was extracted from cells, and each lane contained -25 pg of total RNA. An 1 8S RNA probe was used to normalize the quantity of the RNA
Agtct-Tattgtcaaacttgtccctttccmgccgcaga-Tgaagca Lp3r2 Ip3r1
Immunological selection of variant mouse lymphoid cells with altered glucocorticoid responsiveness.
M. Danielsen (1983)
Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry.
C. Dive (1992)
Anti‐immunoglobulins induce death by apoptosis in WEHI‐231 B lymphoma cells
L. Benhamou (1990)
Mitogen-induced oscillations of cytosolic Ca2+ and transmembrane Ca2+ current in human leukemic T cells.
R. S. Lewis (1989)
Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma.
P. Ralph (1973)
Endogenous nuclease. Properties and effects on transcribed genes in chromatin.
J. Vanderbilt (1982)
Sequence and functional characterization of a third inositol trisphosphate receptor subtype, IP3R-3, expressed in pancreatic islets, kidney, gastrointestinal tract, and other tissues.
O. Blondel (1993)
Increased uptake of calcium by human lymphocytes treated with phytohaemagglutinin.
G. Asherson (1970)
myo-Inositol 1,4,5-trisphosphate mobilizes Ca2+ from isolated adipocyte endoplasmic reticulum but not from plasma membranes.
D. Delfert (1986)
Induction of apoptosis in uninfected lymphocytes by HIV-1 Tat protein.
C. Li (1995)
J. N. Vanderbilt
Immunogold localization of inositol 1, 4, 5-trisphosphate (InsP3) receptor in mouse cerebellar Purkinje cells using three monoclonal antibodies.
H. Otsu (1990)
Calcium-dependent killing of immature thymocytes by stimulation via the CD3/T cell receptor complex.
D. McConkey (1989)
Ion channels activated by inositol 1,4,5-trisphosphate in plasma membrane of human T-lymphocytes
M. Kuno (1987)
Ca2+]i measurement in cells with changes in light scatter reflects only those cells whose membrane integrity was still intact and excluded trypan blue
Structure of a novel InsP3 receptor.
T. Südhof (1991)
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
A. Wyllie (1980)
We thank G. Bell for lP3R3 cDNA and C. Williams for pathology photography
Three additional inositol 1,4,5-trisphosphate receptors: molecular cloning and differential localization in brain and peripheral tissues.
C. Ross (1992)
Lymphoma models for B cell activation and tolerance. VI. Reversal of anti-Ig-mediated negative signaling by T cell-derived lymphokines.
D. Scott (1987)
Glucocorticoid-induced apoptosis in the thymus.
Cohen Jj (1992)
Heterogeneity among T cells in intracellular free calcium responses after mitogen stimulation with PHA or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry.
P. Rabinovitch (1986)
Apoptosis in AIDS.
M. Gougeon (1993)
The effects of calcium, agmatine and phosphate on mitosis in normal and irradiated populations of rat thymocytes.
J. Whitfield (1966)
myo-Inositol 1,4,5-trisphosphate. A second messenger for the hormonal mobilization of intracellular Ca2+ in liver.
S. Joseph (1984)
Odor stimuli trigger influx of calcium into olfactory neurons of the channel catfish.
D Restrepo (1990)
Dexamethasone induces irreversible G1 arrest and death of a human lymphoid cell line
J. Harmon (1979)
Activation of a suicide process of thymocytes through DNA fragmentation by calcium ionophores and phorbol esters.
H. Kizaki (1989)
IP3-activated calcium-permeable channels in the inside-out patches of cultured cerebellar Purkinje cells.
M. Kuno (1994)
A model for receptor-regulated calcium entry.
J. Putney (1986)
in Membrane Receptors of Lymphocytes, M
N. L. Warner (1685)
Steroid sensitivity of thymocyte subpopulations during intrathymic differentiation. Effects of 17 beta-estradiol and dexamethasone on subsets expressing T cell antigen receptor or IL-2 receptor.
I. Screpanti (1989)

This paper is referenced by
Glucocorticoid-induced apoptosis.
C. Distelhorst (1997)
Involvement of Inositol 1,4,5‐Trisphosphate‐Regulated Stores of Intracellular Calcium in Calcium Dysregulation and Neuron Cell Death Caused by HIV‐1 Protein Tat
N. Haughey (1999)
Oncogenic K-Ras suppresses IP3-dependent Ca2+ release through remodelling of the isoform composition of IP3Rs and ER luminal Ca2+ levels in colorectal cancer cell lines
C. Pierro (2014)
The inositol 1,4,5-trisphosphate receptor is required to signal autophagic cell death.
D. Lam (2008)
Signaling pathways between the plasma membrane and endoplasmic reticulum calcium stores
J. Putney (2000)
Apical Vesicles Bearing Inositol 1,4,5-trisphosphate Receptors in the Ca2+Initiation Site of Ductal Epithelium of Submandibular Gland
M. Yamamoto-Hino (1998)
A functional SNP in the NKX2.5-binding site of ITPR3 promoter is associated with susceptibility to systemic lupus erythematosus in Japanese population
T. Oishi (2008)
Specific Gap Junctions Enhance the Neuronal Vulnerability to Brain Traumatic Injury
M. Frantseva (2002)
Altered Calcium Homeostasis in Cells Transformed by Mitochondria from Individuals with Parkinson's Disease
J. Sheehan (1997)
Calcium Homeostasis and Reactive Oxygen Species Production in Cells Transformed by Mitochondria from Individuals with Sporadic Alzheimer’s Disease
J. Sheehan (1997)
Putative mechanism through which N-cadherin-mediated cell contact maintains calcium homeostasis and thereby prevents ovarian cells from undergoing apoptosis.
J. J. Peluso (1997)
Thymocyte selection in Vav and IRF‐1 gene‐deficient mice
J. Penninger (1998)
Consensus report of the 8 and 9th Weinman Symposia on Gene x Environment Interaction in carcinogenesis: novel opportunities for precision medicine
M. Carbone (2018)
Selective down-regulation of IP(3)receptor subtypes by caspases and calpain during TNF alpha -induced apoptosis of human T-lymphoma cells.
F. Diaz (2000)
IP3 receptors in cell survival and apoptosis: Ca2+ release and beyond
S. Joseph (2007)
A critical appraisal of the role of intracellular Ca2+-signaling pathways in Kawasaki disease.
Jeroen Bijnens (2018)
The Ca 2 + signal as common target of three different proteins involved in the apoptotic process
S. D. Ferrara (2010)
Inositol trisphosphate receptor Ca2+ release channels.
J. Foskett (2007)
Organelle–Organelle Contacts
Uri Manor (2020)
Role of calcium in glucocorticosteroid-induced apoptosis of thymocytes and lymphoma cells: resurrection of old theories by new findings.
C. Distelhorst (1998)
Targeting Bcl-2-IP3 receptor interaction to treat cancer: A novel approach inspired by nearly a century treating cancer with adrenal corticosteroid hormones.
C. Distelhorst (2018)
Modulators of calcium signalling in neuronal physiology and disease
J. Grant (2008)
Discrete nondeterministic modeling of biochemical networks
J. Jack (2009)
Dementia Immunodeficiency Virus Type 1-Associated Apoptosis : Implications for Human Receptor-Mediated Neural Signaling and Lymphotropic Virions Affect Chemokine
J. Zheng (1998)
Requirement for cGMP in Nerve Cell Death Caused by Glutathione Depletion
Y. Li (1997)
Apoptosis regulation by Bcl-xL modulation of mammalian inositol 1,4,5-trisphosphate receptor channel isoform gating
Chi Li (2007)
Ion channels in the immune system as targets for immunosuppression.
M. Cahalan (1997)
InsP3Receptor Is Essential for Growth and Differentiation but Not for Vision in Drosophila
J. Acharya (1997)
The Endoplasmic Reticulum Pathway
M. Harr (2009)
Developmental expression of the calcium release channels during early neurogenesis of the mouse cerebral cortex
A. Faure (2001)
Calcium signaling in apoptotic mammalian cells
Y. Lao (2008)
Remodeling of Ca2+ signaling in cancer: Regulation of inositol 1,4,5-trisphosphate receptors through oncogenes and tumor suppressors.
H. Ando (2018)
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