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Sequence Of Conjugative Plasmid PIP1206 Mediating Resistance To Aminoglycosides By 16S RRNA Methylation And To Hydrophilic Fluoroquinolones By Efflux
B. Périchon, P. Bogaerts, T. Lambert, L. Frangeul, P. Courvalin, M. Galimand
Published 2008 · Biology, Medicine
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ABSTRACT Self-transferable IncFI plasmid pIP1206, isolated from an Escherichia coli clinical isolate, carries two new resistance determinants: qepA, which confers resistance to hydrophylic fluoroquinolones by efflux, and rmtB, which specifies a 16S rRNA methylase conferring high-level aminoglycoside resistance. Analysis of the 168,113-bp sequence (51% G+C) revealed that pIP1206 was composed of several subregions separated by copies of insertion sequences. Of 151 open reading frames, 56 (37%) were also present in pRSB107, isolated from a bacterium in a sewage treatment plant. pIP1206 contained four replication regions (RepFIA, RepFIB, and two partial RepFII regions) and a transfer region 91% identical with that of pAPEC-O1-ColBM, a plasmid isolated from an avian pathogenic E. coli. A putative oriT region was found upstream from the transfer region. The antibiotic resistance genes tet(A), catA1, blaTEM-1, rmtB, and qepA were clustered in a 33.5-kb fragment delineated by two IS26 elements that also carried a class 1 integron, including the sulI, qacEΔ1, aad4, and dfrA17 genes and Tn10, Tn21, and Tn3-like transposons. The plasmid also possessed a raffinose operon, an arginine deiminase pathway, a putative iron acquisition gene cluster, an S-methylmethionine metabolism operon, two virulence-associated genes, and a type I DNA restriction-modification (R-M) system. Three toxin/antitoxin systems and the R-M system ensured stabilization of the plasmid in the host bacteria. These data suggest that the mosaic structure of pIP1206 could have resulted from recombination between pRSB107 and a pAPEC-O1-ColBM-like plasmid, combined with structural rearrangements associated with acquisition of additional DNA by recombination and of mobile genetic elements by transposition.
This paper references
Complete nucleotide sequence of Tn10.
R. Chalmers (2000)
Structure and mechanism of multifunctional restriction endonucleases.
R. Yuan (1981)
Regulation of DNA replication by iterons: an interaction between the ori2 and incC regions mediated by RepE‐bound iterons inhibits DNA replication of mini‐F plasmid in Escherichia coli
H. Uga (1999)
Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.
C. Aslanidis (1990)
Construction and analysis of F plasmid traR, trbJ, and trbH mutants.
K. Maneewannakul (1993)
Distribution and in-vitro transfer of tetracycline resistance determinants in clinical and aquatic Acinetobacter strains.
L. Guardabassi (2000)
Complete DNA sequence of a ColBM plasmid from avian pathogenic Escherichia coli suggests that it evolved from closely related ColV virulence plasmids.
T. Johnson (2006)
Y. Nishimura (1993)
CAAT-Box, contigs-Assembly and Annotation Tool-Box for genome sequencing projects
L. Frangeul (2004)
Characterization of the functional sites in the oriT region involved in DNA transfer promoted by sex factor plasmid R100.
T. Abo (1995)
Mutational analysis of the open reading frames in the transposable element IS1.
M. Jakowec (1988)
Effects of the ccd function of the F plasmid on bacterial growth.
A. Jaffe (1985)
A RAPID ALKALINE EXTRACTION PROCEDURE FOR SCREENING RECOMBINANT DNA
H. Birnboim (1979)
Evidence for a repeating domain in type I restriction enzymes.
P. Argos (1985)
Gene structure, organization, expression, and potential regulatory mechanisms of arginine catabolism in Enterococcus faecalis.
Belén Barcelona-Andrés (2002)
Pathways and regulation of bacterial arginine metabolism and perspectives for obtaining arginine overproducing strains
C. Lu (2006)
The porin RafY encoded by the raffinose plasmid pRSD2 of Escherichia coli forms a general diffusion pore and not a carbohydrate-specific porin.
C. Andersen (1998)
Incompatibility groups and the classification of fi - resistance factors.
Y. Chabbert (1972)
Insertion sequences. Microbiol
J Mahillon (1998)
Transposon Tn21, Flagship of the Floating Genome
C. Liebert (1999)
Modulation of DNA supercoiling activity of Escherichia coli DNA gyrase by F plasmid proteins. Antagonistic actions of LetA (CcdA) and LetD (CcdB) proteins.
S. Maki (1992)
Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli.
C. Aslanidis (1989)
A Family of S-Methylmethionine-dependent Thiol/Selenol Methyltransferases
B. Neuhierl (1999)
Analysis of the sequence and gene products of the transfer region of the F sex factor.
L. Frost (1994)
Genetic recombination can generate altered restriction specificity.
F. Fuller-Pace (1984)
Mosaic Structure of p1658/97, a 125-Kilobase Plasmid Harboring an Active Amplicon with the Extended-Spectrum β-Lactamase Gene blaSHV-5
M. Zienkiewicz (2007)
Restriction-modification systems determined by Pseudomonas plasmids.
G. Jacoby (1982)
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
H. Birnboim (1979)
arcD, the first gene of the arc operon for anaerobic arginine catabolism in Pseudomonas aeruginosa, encodes an arginine-ornithine exchanger.
H. Verhoogt (1992)
The 120 592 bp IncF plasmid pRSB107 isolated from a sewage-treatment plant encodes nine different antibiotic-resistance determinants, two iron-acquisition systems and other putative virulence-associated functions.
R. Szczepanowski (2005)
Compatibility Groups among fi− R Factors
N. Datta (1971)
Addiction modules and programmed cell death and antideath in bacterial cultures.
H. Engelberg-Kulka (1999)
Characterization of the arcD arginine:ornithine exchanger of Pseudomonas aeruginosa. Localization in the cytoplasmic membrane and a topological model.
J. P. Bourdineaud (1993)
Konings. 1992. arcD, the first gene of the arc operon for anaerobic arginine catabolism in Pseudomonas aeruginosa, encodes an arginineornithine exchanger
H J C Verhoogt
New Plasmid-Mediated Fluoroquinolone Efflux Pump, QepA, Found in an Escherichia coli Clinical Isolate
Kunikazu Yamane (2007)
Partition mechanism of F plasmid: Two plasmid gene-encoded products and a cis-acting region are involved in partition
T. Ogura (1983)
Transferable Resistance to Aminoglycosides by Methylation of G1405 in 16S rRNA and to Hydrophilic Fluoroquinolones by QepA-Mediated Efflux in Escherichia coli
B. Périchon (2007)
Nucleotide sequence analysis and expression of the minimum REPI replication region and incompatibility determinants of pColV-K30.
J. Perez-Casal (1989)
Two new gene cassettes, dfr17 (for trimethoprim resistance) and aadA4 (for spectinomycin/streptomycin resistance), inserted in an Escherichia coli class 1 integron.
C. Chang (2000)
S-methylmethionine metabolism in Escherichia coli.
M. Thanbichler (1999)
Reconstitution of F Factor DNA Replication in Vitro with Purified Proteins*
S. Zzaman (2004)
Plasmid addiction genes of bacteriophage P1: doc, which causes cell death on curing of prophage, and phd, which prevents host death when prophage is retained.
H. Lehnherr (1993)
Basis for changes in DNA recognition by the EcoR124 and EcoR124/3 type I DNA restriction and modification enzymes.
C. Price (1989)
Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control.
H. Tsutsui (1983)
Nucleotide sequence of the F plasmid leading region.
N. Manwaring (1999)
DNA restriction-modification systems mediate plasmid maintenance.
S. Kulakauskas (1995)
Two DNA recognition domains of the specificity polypeptides of a family of type I restriction enzymes.
F. Fuller-Pace (1986)
Mini-F plasmid genes that couple host cell division to plasmid proliferation.
T. Ogura (1983)
chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100.
Y. Masuda (1993)
CAAT-Box, Contigs-Assembly and Annotation ToolBox for genome sequencing projects. Bioinformatics 20:790–797
L. Frangeul (2004)
Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium.
P. Bogaerts (2007)
Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli.
C. Ulmke (1997)
Factors determining frequency of plasmid cointegration mediated by insertion sequence IS1.
Y. Machida (1982)
A Salmonella dublin virulence plasmid locus that affects bacterial growth under nutrient‐limited conditions
G. Pullinger (1992)
Mosaic structure of plasmids from natural populations of Escherichia coli.
E. F. Boyd (1996)
The arcABDC gene cluster, encoding the arginine deiminase pathway of Bacillus licheniformis, and its activation by the arginine repressor argR.
A. Maghnouj (1998)
J. Mahillon (1998)
The arginine repressor of Escherichia coli.
W. Maas (1994)
Shaping the genome--restriction-modification systems as mobile genetic elements.
I. Kobayashi (1999)
Selfishness and death: raison d'être of restriction, recombination and mitochondria.
I. Kobayashi (1998)
Novel Streptomycin and Spectinomycin Resistance Gene as a Gene Cassette within a Class 1 Integron Isolated from Escherichia coli
D. Sandvang (2000)
The type IC hsd loci of the enterobacteria are flanked by DNA with high homology to the phage P1 genome: implications for the evolution and spread of DNA restriction systems
C. Tyndall (1997)
This paper is referenced by
Status of qnr and aac(6’)-lb-cr Genes in Quinolone-Resistant Non-Fermenters
Apurba Sarkar (2017)
Treatment of Acinetobacter infections
A. Michalopoulos (2010)
Expression of Multidrug Efflux Pump Genes acrAB-tolC, mdfA, and norE in Escherichia coli Clinical Isolates as a Function of Fluoroquinolone and Multidrug Resistance ‡ ‡ Supplemental material for this article may be found at http://dx.doi.org/10.1128/AAC.00996-10.
Michelle C Swick (2010)
Caracterização da resistência de isolados clínicos de Acinetobacter baumannii a antimicrobianos e desinfetante hospitalar
Daniella Cristina Rodrigues Pereira (2013)
Transferable mechanisms of quinolone resistance.
J. Ruiz (2012)
High prevalence of plasmid-mediated 16S rRNA methylase gene rmtB among Escherichia coli clinical isolates from a Chinese teaching hospital
F. Yu (2010)
EnvironmentCattle, Farm Workers, and the Farm Systems in Escherichia coli Circulating -Lactamase and Associated Addiction β Molecular Characterization of CTX-M
Suk-Kyung Lim (2013)
Resistance Plasmid Families in Enterobacteriaceae
A. Carattoli (2009)
Prolonged febrile illness due to CTX-M-15 extended-spectrum β-lactamase-producing Klebsiella pneumoniae infection in Nigeria
O. A. Aboderin (2012)
The Arginine Deiminase Operon Is Responsible for a Fitness Trade-Off in Extended-Spectrum-β-Lactamase-Producing Strains of Escherichia coli
T. Billard-Pomares (2019)
Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures.
A. Fernández-Castané (2012)
F33:A−:B− and F2:A−:B− Plasmids Mediate Dissemination of rmtB-blaCTX-M-9 Group Genes and rmtB-qepA in Enterobacteriaceae Isolates from Pets in China
Y. Deng (2011)
Insights into the evolution of gene organization and multidrug resistance from Klebsiella pneumoniae plasmid pKF3-140.
J. Bai (2013)
Prevalence of 16S rRNA methylase genes in Klebsiella pneumoniae isolates from a Chinese teaching hospital: coexistence of rmtB and armA genes in the same isolate.
Fangyou Yu (2009)
Plasmid-mediated quinolone resistance genes in Enterobacteriaceae isolates associated with community and nosocomial urinary tract infection in East London, UK.
A. Amin (2009)
Complete Nucleotide Sequences of blaCTX-M-Harboring IncF Plasmids from Community-Associated Escherichia coli Strains in the United States
J. Li (2015)
Evolution and dynamics of megaplasmids with genome sizes larger than 100 kb in the Bacillus cereus group
J. Zheng (2013)
Understanding the Shared Bacterial Genome
Jonathan R. Iredell (2010)
Determinants of Listeria monocytogenes stress responses
Anna Pöntinen (2019)
The role of natural environments in the evolution of resistance traits in pathogenic bacteria
J. Martínez (2009)
Dissemination of IncFII plasmids carrying rmtB and qepA in Escherichia coli from pigs, farm workers and the environment.
Y. Deng (2011)
Bacterial toxin-antitoxin systems targeting translation
R. Díaz-Orejas (2010)
Transfer of quinolone resistance gene qnrA1 to Escherichia coli through a 50 kb conjugative plasmid resulting from the splitting of a 300 kb plasmid.
U. Garza-Ramos (2012)
Antimicrobial Drug Efflux Pumps in Escherichia coli
X. Li (2016)
Complete sequence of pEC14_114, a highly conserved IncFIB/FIIA plasmid associated with uropathogenic Escherichia coli cystitis strains.
C. DebRoy (2010)
The IncF plasmid pRSB225 isolated from a municipal wastewater treatment plant's on-site preflooder combining antibiotic resistance and putative virulence functions is highly related to virulence plasmids identified in pathogenic E. coli isolates.
D. Wibberg (2013)
Epigenetic Modifications in Gram-Negative Bacteria and the Induction of Patho-Epigenetic Modifications in Host Tissue
A. Zautner (2013)
Plasmid copy number and qnr gene expression in selection of fluoroquinolone-resistant Escherichia coli.
Dániel Gulyás (2019)
Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants.
L. Villa (2010)
Antibiotic resistance in pulmonary infections: mechanisms and epidemiology
O. Makarewicz (2017)
Transferable Mechanisms of Quinolone Resistance from 1998 Onward
J. Ruiz (2019)
Mechanisms of Resistance to Aminoglycoside Antibiotics: Overview and Perspectives.
Sylvie Garneau-Tsodikova (2016)See more