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Inhibitory Activities Of Quinolones Against DNA Gyrase Of Chlamydia Pneumoniae

Satoshi Ameyama, Yuko Shinmura, Masahiro Takahata

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ABSTRACT The gyrA and gyrB genes of Chlamydia pneumoniae TW-183 were cloned, and their proteins were purified by use of a fusion system with a maltose-binding protein. The 50% inhibitory concentrations of garenoxacin, sparfloxacin, moxifloxacin, gatifloxacin, and levofloxacin were 10.1, 47.5, 39.6, 64.2, and 156.9 μg/ml, respectively, and the MICs against C. pneumoniae TW-183 were 0.008, 0.016, 0.063, 0.125, and 0.25 μg/ml, respectively.