Please confirm you are human (Sign Up for free to never see this)
← Back to Search
MerA Gene Expression In Aquatic Environments Measured By MRNA Production And Hg(II) Volatilization.
S. Nazaret, W. Jeffrey, E. Saouter, R. Von Haven, T. Barkay
Published 1994 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
The relationship of merA gene expression (specifying the enzyme mercuric reductase) to mercury volatilization in aquatic microbial communities was investigated with samples collected at a mercury-contaminated freshwater pond, Reality Lake, in Oak Ridge, Tenn. Levels of merA mRNA transcripts and the rate of inorganic mercury [Hg(II)] volatilization were related to the concentration of mercury in the water and to heterotrophic activity in field samples and laboratory incubations of pond water in which microbial heterotrophic activity and Hg(II) concentration were manipulated. Levels of merA-specific mRNA and Hg(II) volatilization were influenced more by microbial metabolic activity than by the concentration of mercury. merA-specific transcripts were detected in some samples which did not reduce Hg(II), suggesting that rates of mercury volatilization in environmental samples may not always be proportional to merA expression.
This paper references
Detection of polychlorinated biphenyl degradation genes in polluted sediments by direct DNA extraction and polymerase chain reaction.
R. W. Erb (1993)
Mercuric reductase: homology to glutathione reductase and lipoamide dehydrogenase. Iodoacetamide alkylation and sequence of the active site peptide.
B. Fox (1983)
Environmental significance of the potential for mer(Tn21)-mediated reduction of Hg2+ to Hg0 in natural waters.
T. Barkay (1989)
Distribution of DNA Sequences Encoding Narrow- and Broad-Spectrum Mercury Resistance.
P. Rochelle (1991)
A mer-lux transcriptional fusion for real-time examination of in vivo gene expression kinetics and promoter response to altered superhelicity.
C. W. Condee (1992)
Mercuric reductase from R-plasmid NR1: characterization and mechanistic study.
S. J. Rinderle (1983)
Ultrasensitivity and heavy-metal selectivity of the allosterically modulated MerR transcription complex.
D. M. Ralston (1990)
Bioluminescent sensors for detection of bioavailable Hg(II) in the environment.
O. Selifonova (1993)
Plasmid-borne mercury resistance in aquatic bacteria
M. Jobling (1988)
Whole-Cell Hybridization of Frankia Strains with Fluorescence- or Digoxigenin-Labeled, 16S rRNA-Targeted Oligonucleotide Probes.
D. Hahn (1993)
Picomolar mercury measurements in seawater and other materials using stannous chloride reduction and two-stage gold amalgamation with gas phase detection
G. Gill (1987)
Synthesis and degradation of the mRNA of the Tn21 mer operon.
B. Gambill (1992)
Regulation of the Staphylococcus aureus plasmid pI258 mercury resistance operon.
L. Chu (1992)
Analyses of organic and inorganic mercury by atomic fluorescence spectrometry using a semiautomatic analytical system
E. Saouter (1994)
Rapid technique for the breakdown of organic mercury compounds in natural waters and effluents
B. J. Farey (1978)
Detection of Gene Expression in Genetically Engineered Microorganisms and Natural Phytoplankton Populations in the Marine Environment by mRNA Analysis.
S. L. Pichard (1991)
The distribution and divergence of DNA sequences related to the Tn21 and Tn501 mer operons.
M. Gilbert (1988)
Mercuric reductase in environmental gram-positive bacteria sensitive to mercury.
E. Bogdanova (1992)
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.
D. Higgins (1988)
Improved Method for Recovery of mRNA from Aquatic Samples and Its Application to Detection of mer Expression.
W. Jeffrey (1994)
Natural transformation of a marine Vibrio by plasmid DNA
W. H. Jeffrey (1990)
The use of DAPI for identifying and counting aquatic microflora1
K. Porter (1980)
Organization, expression, and evolution of genes for mercury resistance.
A. Summers (1986)
Genes encoding mercuric reductases from selected gram-negative aquatic bacteria have a low degree of homology with merA of transposon Tn501.
T. Barkay (1990)
Gene expression per gene dose, a specific measure of gene expression in aquatic microorganisms.
S. L. Pichard (1993)
Estimating bacterial production in marine waters from the simultaneous incorporation of thymidine and leucine.
G. Chin-Leo (1988)
Mercuric reductase. Purification and characterization of a transposon-encoded flavoprotein containing an oxidation-reduction-active disulfide.
B. Fox (1982)
The Isolation of a Mercuric Ion-reducing Flavoprotein from Thiobacillus ferrooxidans
J. Booth (1984)
SYSTAT: statistic, version
The influence of age and multimorbidity on the pharmacokinetics and metabolism of spironolactone.
D. Platt (1984)
Multiple aligned sequence editor (MASE).
D. Faulkner (1988)
Molecular characterization of the gor gene encoding glutathione reductase from Pseudomonas aeruginosa: determinants of substrate specificity among pyridine nucleotide‐disulphide oxidoreductases
A. C. F. Perr (1991)
Rapid method for direct extraction of mRNA from seeded soils.
Y. L. Tsai (1991)
Improved method for recovery of mRNA from aquatic samples: application to detecting mer gene expression
W. H. Jeffrey (1994)
Transcriptional regulation of the mercury-resistance genes of transposon Tn501.
P. Lund (1986)
Bioluminescent Sensors forDetection ofBioavailable Hg(II) intheEnvironmentt
Olga V. Selifonova (1993)
Metabolic growth rate control in Escherichia coli may be a consequence of subsaturation of the macromolecular biosynthetic apparatus with substrates and catalytic components.
K. Jensen (1990)
Effects of Hg, CH(3)-Hg, and Temperature on the Expression of Mercury Resistance Genes in Environmental Bacteria.
Y. L. Tsai (1990)
Untwist and shout: a heavy metal-responsive transcriptional regulator.
A. Summers (1992)
Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants: mer genes and Hg2+ resistance.
T. Barkay (1989)
Comparison of methods to measure acute metal and organometal toxicity to natural aquatic microbial communities.
R. Jonas (1984)
Organomercurial-volatilizing bacteria in the mercury-polluted sediment of Minamata Bay, Japan.
K. Nakamura (1990)
This paper is referenced by
Interactions between metals and microbial communities in
J. Sorci (2006)
Omics in bioremediation: A consolidated overview
Ranjith N. Kumavath (2012)
Mercury in water and biomass of microbial communities in hot springs of Yellowstone National Park, USA
S. King (2006)
Environmental Conditions Constrain the Distribution and Diversity of Archaeal merA in Yellowstone National Park, Wyoming, U.S.A.
Yanping Wang (2011)
Bacterial mercury resistance from atoms to ecosystems.
T. Barkay (2003)
Use of Molecular Probing to Assess Microbial Activities in Natural Ecosystems
J. Gottschal (1997)
Molecular ecology of aquatic communities: reflections and future directions
J. Zehr (2004)
Molecular Approaches to Studies of the Activities of Marine Organisms
J. Zehr (1998)
Stable isotope fractionation analysis as a tool to monitor biodegradation in contaminated acquifers.
R. Meckenstock (2004)
Molecular markers of heavy metal toxicity--a new paradigm for health risk assessment.
S. Yoon (2008)
The use of reporter genes in the study of microbial ecology
S. Lindow (1995)
Microbial generation of elemental mercury from dissolved methylmercury in seawater.
Cheng-Shiuan Lee (2019)
Direct Correlation between Rates of Anaerobic Respiration and Levels of mRNA for Key Respiratory Genes in Geobacter sulfurreducens
Kuk-Jeong Chin (2004)
Role of organic amendments on enhanced bioremediation of heavy metal(loid) contaminated soils.
Jin Hee Park (2011)
Comparative effects of mercury contamination and wastewater effluent input on Gram-negative merA gene abundance in mudflats of an anthropized estuary (Seine, France): a microcosm approach.
J. Ramond (2009)
Characterization and potential application in mercury bioremediation of highly mercury-resistant marine bacterium Bacillus thuringiensis PW-05
H. R. Dash (2013)
Scientific Swift in Bioremediation: An Overview
R. Kumavath (2013)
Section 1 update: Extraction of microbial RNA from aquatic sources: Marine environments
S. L. Pichard (2004)
Assessing the correlation between anaerobic toluene degradation activity and bssA concentrations in hydrocarbon-contaminated aquifer material
Sufia K. Kazy (2010)
In Situ Expression of nifD in Geobacteraceae in Subsurface Sediments
D. Holmes (2004)
Role of the bacterial organomercury lyase (MerB) in controlling methylmercury accumulation in mercury-contaminated natural waters.
J. Schaefer (2004)
Quantifying Expression of a Dissimilatory (bi)Sulfite Reductase Gene in Petroleum-Contaminated Marine Harbor Sediments
Kuk-Jeong Chin (2007)
Detection and Diversity of Expressed Denitrification Genes in Estuarine Sediments after Reverse Transcription-PCR Amplification from mRNA
B. Nogales (2002)
Biological and photochemical production of dissolved gaseous mercury in a boreal lake
Alexandre J. Poulain (2004)
Reductive Dehalogenase Gene Expression as a Biomarker for Physiological Activity of Dehalococcoides spp
P. Lee (2006)
Identification and characterisation of bacterial genes associated with resistance to and/or degradation of environmental pollutants
Belinda L. Davis (2011)
Interactions between metals and microbial communities in New Bedford Harbor, Massachusetts.
T. Ford (1998)
Response of marine microbial communities to anthropogenic stress
T. E. Ford (2000)
Mercury induced community tolerance in microbial biofilms is related to pollution gradients in a long-term polluted river.
Manca Kovac Virsek (2013)
Analysis of mercuric reductase (merA) gene diversity in an anaerobic mercury-contaminated sediment enrichment.
S. M. Ni Chadhain (2006)
Molecular assays advance understanding of sulfate reduction despite cryptic cycles
B. Reese (2013)
Co-selection of metal and antibiotic resistance in the gut microbial community of the Mummichog fish (Fundulus heteroclitus)
Nicole A. Lloyd (2018)See more