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Genetically Modified Ruminal Bacteria Protect Sheep From Fluoroacetate Poisoning

Keith Gregg, Brenton Hamdorf, Kerrin Henderson, Jan Kopecny, Cheryl Wong

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ABSTRACT Four strains of Butyrivibrio fibrisolvens , transformed with a gene encoding fluoroacetate dehalogenase, maintained a combined population of 10 6 to 10 7 cells ml −1 in the rumens of test sheep. Five inoculated sheep showed markedly reduced toxicological symptoms after fluoroacetate poisoning when behavioral, physiological, and histological effects were compared with those of five uninoculated control sheep.