Online citations, reference lists, and bibliographies.
← Back to Search

Gamma Interferon-induced Nitric Oxide Production Reduces Chlamydia Trachomatis Infectivity In McCoy Cells

J. Mayer, M. Woods, Z. Vavr̆ín, J. Hibbs
Published 1993 · Medicine, Biology

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
McCoy cells, murine-derived cells commonly used for propagation of chlamydiae, were found to be efficient producers of nitric oxide (NO) when primed with murine gamma interferon (IFN-gamma) and then exposed to the second signals provided by Escherichia coli lipopolysaccharide, human interleukin-1 alpha, murine tumor necrosis factor alpha, or Chlamydia trachomatis type H. Murine recombinant IFN-gamma over a range of 0 to 50 U/ml inhibited infectivity of C. trachomatis type H in a dose-dependent fashion in McCoy cells while simultaneously inducing NO production. Quantitation of infectious chlamydia progeny remaining in McCoy cells 48 or 72 h postinfection revealed that IFN-gamma-primed McCoy cells reduced chlamydial inclusion-forming units (expressed as units per milliliter) by 4 log10 units at higher IFN-gamma concentrations (50 U/ml) compared with control values. The magnitude of this antichlamydial effect was directly related to increased synthesis of NO, the production of which was IFN-gamma dose dependent. The antichlamydial effects of IFN-gamma were blocked in a dose-dependent manner by the addition of N-guanidino-monomethyl L-arginine (MLA), an inhibitor of nitric oxide synthesis. These results suggest that although IFN-gamma priming of McCoy cells is required for antichlamydial activity, nitric oxide is a necessary effector molecule involved in the mechanism(s) of IFN-gamma-induced inhibition of chlamydial proliferation in this murine cell line. The ability to block the potent antichlamydial effects of IFN-gamma by inhibition of a specific enzyme, nitric oxide synthase, may give insights into mechanisms by which IFN-gamma and perhaps other cytokines are able to control proliferation of chlamydiae and other intracellular pathogens.
This paper references
The prevention of blindness from trachoma.
B. R. Jones (1975)
The prevention of blindness from trachoma.
J. Br (1975)
Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis
H. Caldwell (1981)
A rapid method for the assay of nitrate in urine using the nitrate reductase enzyme of Escherichia coli.
B. Bartholomew (1984)
Inhibition of growth of Chlamydia trachomatis by human gamma interferon
Y. Shemer (1985)
Synthesis of protein in host-free reticulate bodies of Chlamydia psittaci and Chlamydia trachomatis
T. Hatch (1985)
The anti-chlamydial and anti-proliferative activities of recombinant murine interferon-gamma are not dependent on tryptophan concentrations.
L. M. de la Maza (1985)
Interferon-induced inhibition of Chlamydia trachomatis: dissociation from antiviral and antiproliferative effects
L. M. de la Maza (1985)
Chlamydia trachomatis infections. Policy guidelines for prevention and control.
Murine cytotoxic activated macrophages inhibit aconitase in tumor cells. Inhibition involves the iron-sulfur prosthetic group and is reversible.
J. C. Drapier (1986)
Induction of tryptophan catabolism is the mechanism for gamma-interferon-mediated inhibition of intracellular Chlamydia psittaci replication in T24 cells
G. Byrne (1986)
Ultrastructural analysis of the anti-chlamydial activity of recombinant murine interferon-gamma.
L. M. de la Maza (1987)
Macrophage synthesis of nitrite, nitrate, and N-nitrosamines: precursors and role of the respiratory burst.
R. Iyengar (1987)
L-arginine is required for expression of the activated macrophage effector mechanism causing selective metabolic inhibition in target cells.
J. Hibbs (1987)
Macrophage cytotoxicity: role of L-arginine deiminase activity and imino nitrogen oxidation to nitrite
J. B. Hibbs (1987)
Differentiation of murine macrophages to express nonspecific cytotoxicity for tumor cells results in L-arginine-dependent inhibition of mitochondrial iron-sulfur enzymes in the macrophage effector cells.
J. C. Drapier (1988)
Interferon‐γ and tumor necrosis factor induce the L‐arginine‐dependent cytotoxic effector mechanism in murine macrophages*
J. C. Drapier (1988)
Nitric oxide: a cytotoxic activated macrophage effector molecule.
J. Hibbs (1988)
Role in vivo for gamma interferon in control of pneumonia caused by Chlamydia trachomatis in mice.
D. M. Williams (1988)
Mitochondrial iron loss from leukemia cells injured by macrophages. A possible mechanism for electron transport chain defects.
M. Wharton (1988)
In vivo-activated mononuclear phagocytes and protective immunity to chlamydiae in mice
R. Huebner (1988)
SHORT COMMUNICATION: Cytokines Induce an L‐Arginine‐Dependent Effector System in Nonmacrophage Cells
Ina J. Amber (1988)
Recombinant murine gamma interferon inhibits Chlamydia trachomatis serovar L1 in vivo
G. Zhong (1988)
Gamma interferon-mediated cytotoxicity related to murine Chlamydia trachomatis infection
G. Byrne (1988)
Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production.
A. Ding (1988)
Role of tryptophan degradation in respiratory burst-independent antimicrobial activity of gamma interferon-stimulated human macrophages
H. Murray (1989)
Macrophage cytotoxicity against schistosomula of Schistosoma mansoni involves arginine-dependent production of reactive nitrogen intermediates.
S. James (1989)
Antiproliferative activity of gamma-interferon combined with lipopolysaccharide on murine adenocarcinoma: dependence on an L-arginine metabolism with production of nitrite and citrulline.
M. Lepoivre (1989)
Characterization of gamma interferon-mediated cytotoxicity to chlamydia-infected fibroblasts
G. Byrne (1989)
Role of endogenous gamma interferon in host defense against Chlamydia trachomatis infections
G. Zhong (1989)
Reversion of the antichlamydial effect of tumor necrosis factor by tryptophan and antibodies to beta interferon
Y. Shemer-Avni (1989)
Regulation of murine lymphokine production in vivo. III. The lymphoid tissue microenvironment exerts regulatory influences over T helper cell function
R. Daynes (1990)
Leishmania major amastigotes initiate the L-arginine-dependent killing mechanism in IFN-gamma-stimulated macrophages by induction of tumor necrosis factor-alpha.
S. J. Green (1990)
EPR demonstration of iron-nitrosyl complex formation by cytotoxic activated macrophages.
J. Lancaster (1990)
Synthesis of nitric oxide from a terminal guanidino nitrogen atom of L-arginine: A molecular mechanism regulating cellular proliferation that targets intracellular iron
J. Hibbs (1990)
Alterations of ribonucleotide reductase activity following induction of the nitrite-generating pathway in adenocarcinoma cells.
M. Lepoivre (1990)
Macrophage killing of Leishmania parasite in vivo is mediated by nitric oxide from L-arginine.
F. Liew (1990)
Activated macrophages destroy intracellular Leishmania major amastigotes by an L-arginine-dependent killing mechanism.
S. J. Green (1990)
Metabolic fate of L-arginine in relation to microbiostatic capability of murine macrophages.
D. Granger (1990)
Laboratory diagnosis of Chlamydia trachomatis infection.
E. Caul (1990)
IFN--y activated macrophages: detection by electron paramagnetic resonance of complexes between L-arginine-derived nitric oxide and nonheme iron proteins
C. Pellat (1990)
Microbiostatic effect of murine macrophages for Toxoplasma gondii: role of synthesis of inorganic nitrogen oxides from L-arginine
L B Adams (1990)
L-arginine-dependent macrophage effector functions inhibit metabolic activity of Mycobacterium leprae.
L. Adams (1991)
Isolation and initial characterization of a series of Chlamydia trachomatis isolates selected for hydroxyurea resistance by a stepwise procedure
G. Tipples (1991)
Killing of Leishmania Parasites in Activated Murine Macrophages is Based on an L‐Arginine‐Dependent Process That Produces Nitrogen Derivatives
J. Mauel (1991)
Interferon-gamma-treated murine macrophages inhibit growth of tubercle bacilli via the generation of reactive nitrogen intermediates.
M. Denis (1991)
L‐Arginine‐dependent destruction of intrahepatic malaria parasites in response to tumor necrosis factor and/or interleukin 6 stimulation
A. Nüssler (1991)
Killing of virulent Mycobacterium tuberculosis by reactive nitrogen intermediates produced by activated murine macrophages
J. Chan (1992)

This paper is referenced by
Links between Infections, Lung Cancer, and the Immune System
Liviuta Budisan (2021)
Nitric oxide for the prevention and treatment of viral, bacterial, protozoal and fungal infections
P. Bath (2021)
Molecular Mechanisms Contributing Bacterial Infections to the Incidence of Various Types of Cancer
S. Sheweita (2020)
Cell Intrinsic Factors Modulate the Effects of IFNγ on the Development of Chlamydia trachomatis.
S. Sherchand (2016)
The role of integrins and human IDO in the immune response to Chlamydia trachomatis
Sergio J. Davila (2016)
Pathoadaptation of the Intracellular Bacteria Shigella and Chlamydia: Virulence, Antivirulence, and Tissue Tropism
K. Bliven (2015)
Integrin α4β1 Is Necessary for CD4+ T Cell–Mediated Protection against Genital Chlamydia trachomatis Infection
Sergio J. Davila (2014)
Immunity to Chlamydia trachomatis and Host-Pathogen Interactions During Infection
Andrew J. Olive (2014)
Immunology of Genital Tract Infections
E. Weissenbacher (2014)
Chlamydia trachomatis among women with normal and abnormal cervical smears in Lagos, Nigeria -
M. A. Adegbesan-Omilabu (2014)
pneumoniaeto Infection with Chlamydia in the Innate γ Regulation and Role of IFN
Hans Wigzell (2013)
Characterization of the activity and expression of arginine decarboxylase in human and animal Chlamydia pathogens.
Kimberly A. Bliven (2012)
Prolonging Organ Allograft Survival
G. Pieper (2012)
Cryo-electron tomography of Chlamydia trachomatis gives a clue to the mechanism of outer membrane changes.
Z. Huang (2010)
Association Between Chlamydia pneumoniae Antibodies and Lung Cancer: A Meta-Analysis
Zhuang Liu (2010)
The NOD/RIP2 Pathway Is Essential for Host Defenses Against Chlamydophila pneumoniae Lung Infection
K. Shimada (2009)
Resistance to peroxynitrite in Neisseria gonorrhoeae.
Kenneth R. Barth (2009)
Immunity Against Chlamydia trachomatis
E. Marks (2008)
Long-term effects of amino acids on chlamydia infections
Joscha Gussmann (2008)
Caractérisation des interactions glycosaminoglycannes/protéines dans le but de développer des molécules d'intérêt thérapeutique : exemples de l'Endocan et de l'interféron gamma
Stéphane Sarrazin (2007)
Immunology of the genital tract - a review
M. Wirth (2007)
Upregulation of IFN-gamma receptor expression by proinflammatory cytokines influences IDO activation in epithelial cells.
K. A. Shirey (2006)
Comparison of Gamma Interferon-Mediated Antichlamydial Defense Mechanisms in Human and Mouse Cells
C. Roshick (2006)
Chlamydia trachomatis modulates expression of tumor suppressor gene caveolin-1 and oncogene C-myc in the transformation zone of non-neoplastic cervical tissue.
T. Schlott (2005)
Human and Mouse Cells Antichlamydial Defense Mechanisms in Comparison of Gamma Interferon-Mediated
C. Roshick (2005)
Nitric Oxide and Chronic HCV and HIV Infections
G. Lake-Bakaar (2004)
Interferons in immunity to chlamydia pneumoniae
A. Rothfuchs (2004)
K. Kelly (2003)
Antimicrobial Properties of Nitric Oxide
M. Degroote (2002)
Production of tumour necrosis factor-alpha (TNF-alpha) and reactive nitrogen intermediates by ovine peripheral blood leucocytes stimulated by Ehrlichia (Cytoecetes) phagocytophila.
H. Gokce (2002)
A Ruthenium (III) Polyaminocarboxylate Complex, A Novel Nitric Oxide Scavenger, Enhances Graft Survival and Decreases Nitrosylated Heme Protein in Models of Acute and Delayed Cardiac Transplant Rejection
G. Pieper (2002)
Chlamydia trachomatis Persistence in the Female Mouse Genital Tract: Inducible Nitric Oxide Synthase and Infection Outcome
K. Ramsey (2001)
See more
Semantic Scholar Logo Some data provided by SemanticScholar