Online citations, reference lists, and bibliographies.
← Back to Search

Genetic And Molecular Characterization Of Tn21, A Multiple Resistance Transposon From R100.1.

F. de la Cruz, J. Grinsted
Published 1982 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Tge transposon Tn21 has been transposed from R100.1 to plasmid pACYC184 and, from the resulting recombinants, to plasmid R388. The sites of insertion and the orientation of the element in several pACYC184::Tn21 recombinants have been examined. Restriction enzyme analysis of these recombinants has resulted in a detailed map of Tn21; this is compared with the published maps of the relevant part of R100.1. Heteroduplex analysis has shown short inverted repeat sequences at the ends of the element. With various in vitro-generated deletion mutants of Tn21, the internal gene necessary for transposition (tnpA) was localized within the terminal 4.3 kilobases of the right-hand end of the element. Genetic analysis of transposition of Tn21 suggests that the process proceeds via cointegrates. Since the end products of transposition are simple recombinants of the element and the recipient replicon, Tn21 must contain a gene that codes for a resolvase type of activity (tnpR gene).



This paper is referenced by
10.1111/J.1574-6968.1987.TB02108.X
Mechanisms involved in the formation of plaque-forming derivatives from over-sized hybrid phages between bacteriophage P1 and the R plasmid NR1
S. Iida (1987)
10.1002/AOC.590030402
Mercury resistance and detoxification in bacteria
B. H. Belliveau (1989)
10.1128/AAC.00982-07
Different Pathways to Acquiring Resistance Genes Illustrated by the Recent Evolution of IncW Plasmids
C. Revilla (2008)
10.1007/BF00332761
Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase
P. Avila (2004)
10.1007/s004380050432
Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation
E. Cabezón (1997)
10.1128/AAC.45.4.1263-1270.2001
Transposons Tn1696 and Tn21and Their Integrons In4 and In2 Have Independent Origins
S. Partridge (2001)
10.1128/JB.185.21.6371-6384.2003
The IS1111 family members IS4321 and IS5075 have subterminal inverted repeats and target the terminal inverted repeats of Tn21 family transposons.
S. Partridge (2003)
10.1007/BF00333400
Identification and characterization of Tn4653, a transposon covering the toluene transposon Tn4651 on TOL plasmid pWW0
M. Tsuda (2004)
10.1016/B978-0-08-042000-4.50008-5
3 – Horizontal gene transfer in bacterial soil communities and its significance for risk assessment
F. R. Schmidt (1992)
10.1128/AAC.35.8.1576
Translocation of antibiotic resistance determinants including an extended-spectrum beta-lactamase between conjugative plasmids of Klebsiella pneumoniae and Escherichia coli.
D. Sirot (1991)
10.1101/726927
Comparative analysis of MOBQ4 plasmids demonstrates that MOBQ is a cis-acting-enriched relaxase protein family
M. P. Garcillán-Barcia (2019)
10.1016/0147-619X(88)90015-7
The distribution and divergence of DNA sequences related to the Tn21 and Tn501 mer operons.
M. Gilbert (1988)
10.1007/BF02464882
On the evolution of Tn21-like multiresistance transposons: Sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette
W. Wohlleben (2006)
10.1046/j.1365-2958.1997.6091980.x
Structure and function of 59‐base element recombination sites associated with mobile gene cassettes
H. W. Stokes (1997)
10.1002/biot.200800184
Changing the recognition site of a conjugative relaxase by rational design.
B. González-Pérez (2009)
10.1128/AAC.39.11.2499
Antimicrobial and mercury resistance in aerobic gram-negative bacilli in fecal flora among persons with and without dental amalgam fillings.
M. Osterblad (1995)
10.1016/j.femsec.2003.12.011
Dissemination of TnMERI1-like mercury resistance transposons among Bacillus isolated from worldwide environmental samples.
M. Narita (2004)
10.1089/mdr.2010.0042
Transposons related to Tn1696 in IncHI2 plasmids in multiply antibiotic resistant Salmonella enterica serovar Typhimurium from Australian animals.
Amy K. Cain (2010)
10.1007/978-94-011-6565-5_9
Transposition-Like Events Mediated by Single-Ended Derivatives of Transposon Tn21
F. Cruz (1985)
10.1007/BF00330610
Transposon Tn21 encodes a RecA-independent site-specific integration system
E. N. Martínez (2004)
10.1128/JB.180.22.6039-6042.1998
The carboxyl terminus of protein TraD adds specificity and efficiency to F-plasmid conjugative transfer.
J. I. Sastre (1998)
10.1093/nar/gku741
A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation
J. D. Carballeira (2014)
10.1016/0147-619X(88)90007-8
Factors that affect transposition mediated by the Tn21 transposase.
J. Grinsted (1988)
10.1128/AAC.36.5.1049
Nucleotide sequence of the ampC-ampR region from the chromosome of Yersinia enterocolitica.
A. Seoane (1992)
10.1128/AAC.43.10.2538
Reconstruction of an Active Integron Recombination Site after Integration of a Gene Cassette at a Secondary Site
H. Segal (1999)
10.1016/0378-1097(90)90045-R
Detection of small genotypic changes in Escherichia coli by pyrolysis mass spectroscopy
R. Goodacre (1990)
10.1016/B978-0-12-638680-6.50009-0
CHAPTER 5 – Tn3 and Its Relatives
F. Heffron (1983)
10.1111/j.1365-2958.1992.tb01467.x
Gene cassettes from the insert region of integrons are excised as covalently closed circles
C. M. Collis (1992)
10.1099/00221287-139-8-1761
The ancestral IncP replication system consisted of contiguous oriV and trfA segments as deduced from a comparison of the nucleotide sequences of diverse IncP plasmids.
C. Smith (1993)
10.1074/JBC.M207250200
Purification and Properties of TrwB, a Hexameric, ATP-binding Integral Membrane Protein Essential for R388 Plasmid Conjugation*
Itsaso Hormaeche (2002)
Estudio comparativo de sistemas de secreción tipo IV implicados en transferencia conjugativa de DNA y virulencia bacteriana
D. Paz (2010)
10.1038/s41598-019-46318-1
A ParDE-family toxin antitoxin system in major resistance plasmids of Enterobacteriaceae confers antibiotic and heat tolerance
M. Kamruzzaman (2019)
See more
Semantic Scholar Logo Some data provided by SemanticScholar